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OPN5 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  OPN5
Gene Name:  opsin 5
Sequence             
Original Clone ID: ccsbBroadEn_05255
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC126194.1,BC126198.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00510710

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1063nts         Open reading frame : 1 to 1063
Reference Sequence Alignment

ATGGCGTTAAATCACACTGCCCTGCCTCAGGACGAGCGCCTGCCCCATTACCTTCGAGAT
GGGGATCCTTTTGCTTCCAAACTTTCTTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTA
ACAATAATTGGGATTCTGTCCACATTTGGAAATGGATATGTCCTTTACATGTCTTCTAGA
CGAAAGAAGAAGCTGAGACCCGCTGAAATAATGACTATCAATTTAGCAGTCTGTGATCTG
GGGATTTCAGTTGTAGGCAAGCCGTTCACCATCATCTCTTGCTTTTGTCACCGCTGGGTG
TTTGGCTGGATCGGCTGCCGCTGGTATGGATGGGCTGGATTTTTCTTTGGCTGTGGAAGC
CTTATCACCATGACTGCTGTCAGCCTGGATCGATATTTGAAAATCTGCTATTTATCTTAT
GGGGTTTGGCTGAAAAGAAAGCACGCCTACATCTGCCTGGCAGCCATCTGGGCCTATGCT
TCCTTCTGGACCACCATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGA
ACCTCGTGCACCCTGGACTGGTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTTCATC
CTGAACATCCTCTTCTTCTGCCTCTTGCTCCCAACGGCTGTGATCGTGTTCTCCTACGTA
AAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGAAGTAGCTCATTTCGACAGTCGGATC
CATAGCAGCCATGTGCTGGAAATGAAACTGACAAAGGTAGCGATGTTGATTTGTGCTGGA
TTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCA
GACTCCATTCCCATACAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATG
TACAATCCCATCATTTACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGT
TTGAAAGCAACCAAGAAGAAGTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTC
AGGAAGTCTTCTGCTGTGCTGGAAATTCATGAAGAGTGGGAA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : OPN5
Symbol Nomenclature : OPN5
SYNONYM : GPR136; PGR12; TMEM13
Designation : G-protein coupled receptor 136|neuropsin|opsin-5|transmembrane protein 13
Full Nomenclature : opsin 5
GENEID : 221391
GenBank Accession : HQ258199
HGNC : 19992
MIM : 609042
Target GenBank: HQ258199

 

Reference Sequence Alignment

  

HsCD00510710      ATGGCGTTAAATCACACTGCCCTGCCTCAGGACGAGCGCCTGCCCCATTACCTTCGAGAT
NM_181744.3       ATGGCGTTAAATCACACTGCCCTGCCTCAGGACGAGCGCCTGCCCCATTACCTTCGAGAT
                  ************************************************************

HsCD00510710      GGGGATCCTTTTGCTTCCAAACTTTCTTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTA
NM_181744.3       GGGGATCCTTTTGCTTCCAAACTTTCTTGGGAAGCGGATTTAGTGGCTGGCTTTTACCTA
                  ************************************************************

HsCD00510710      ACAATAATTGGGATTCTGTCCACATTTGGAAATGGATATGTCCTTTACATGTCTTCTAGA
NM_181744.3       ACAATAATTGGGATTCTGTCCACATTTGGAAATGGATATGTCCTTTACATGTCTTCTAGA
                  ************************************************************

HsCD00510710      CGAAAGAAGAAGCTGAGACCCGCTGAAATAATGACTATCAATTTAGCAGTCTGTGATCTG
NM_181744.3       CGAAAGAAGAAGCTGAGACCCGCTGAAATAATGACTATCAATTTAGCAGTCTGTGATCTG
                  ************************************************************

HsCD00510710      GGGATTTCAGTTGTAGGCAAGCCGTTCACCATCATCTCTTGCTTTTGTCACCGCTGGGTG
NM_181744.3       GGGATTTCAGTTGTAGGCAAGCCGTTCACCATCATCTCTTGCTTTTGTCACCGCTGGGTG
                  ************************************************************

HsCD00510710      TTTGGCTGGATCGGCTGCCGCTGGTATGGATGGGCTGGATTTTTCTTTGGCTGTGGAAGC
NM_181744.3       TTTGGCTGGATCGGCTGCCGCTGGTATGGATGGGCTGGATTTTTCTTTGGCTGTGGAAGC
                  ************************************************************

HsCD00510710      CTTATCACCATGACTGCTGTCAGCCTGGATCGATATTTGAAAATCTGCTATTTATCTTAT
NM_181744.3       CTTATCACCATGACTGCTGTCAGCCTGGATCGATATTTGAAAATCTGCTATTTATCTTAT
                  ************************************************************

HsCD00510710      GGGGTTTGGCTGAAAAGAAAGCACGCCTACATCTGCCTGGCAGCCATCTGGGCCTATGCT
NM_181744.3       GGGGTTTGGCTGAAAAGAAAGCACGCCTACATCTGCCTGGCAGCCATCTGGGCCTATGCT
                  ************************************************************

HsCD00510710      TCCTTCTGGACCACCATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGA
NM_181744.3       TCCTTCTGGACCACCATGCCCTTGGTAGGTCTGGGGGACTACGTACCTGAGCCCTTCGGA
                  ************************************************************

HsCD00510710      ACCTCGTGCACCCTGGACTGGTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTTCATC
NM_181744.3       ACCTCGTGCACCCTGGACTGGTGGCTGGCCCAGGCCTCGGTAGGGGGCCAGGTTTTCATC
                  ************************************************************

HsCD00510710      CTGAACATCCTCTTCTTCTGCCTCTTGCTCCCAACGGCTGTGATCGTGTTCTCCTACGTA
NM_181744.3       CTGAACATCCTCTTCTTCTGCCTCTTGCTCCCAACGGCTGTGATCGTGTTCTCCTACGTA
                  ************************************************************

HsCD00510710      AAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGAAGTAGCTCATTTCGACAGTCGGATC
NM_181744.3       AAGATCATTGCCAAGGTTAAGTCCTCTTCCAAAGAAGTAGCTCATTTCGACAGTCGGATC
                  ************************************************************

HsCD00510710      CATAGCAGCCATGTGCTGGAAATGAAACTGACAAAGGTAGCGATGTTGATTTGTGCTGGA
NM_181744.3       CATAGCAGCCATGTGCTGGAAATGAAACTGACAAAGGTAGCGATGTTGATTTGTGCTGGA
                  ************************************************************

HsCD00510710      TTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCA
NM_181744.3       TTCCTGATTGCCTGGATTCCTTATGCAGTGGTGTCTGTGTGGTCAGCTTTTGGAAGGCCA
                  ************************************************************

HsCD00510710      GACTCCATTCCCATACAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATG
NM_181744.3       GACTCCATTCCCATACAGCTCTCTGTGGTGCCAACCCTACTTGCAAAATCTGCAGCGATG
                  ************************************************************

HsCD00510710      TACAATCCCATCATTTACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGT
NM_181744.3       TACAATCCCATCATTTACCAAGTTATTGATTACAAATTTGCCTGTTGCCAAACTGGTGGT
                  ************************************************************

HsCD00510710      TTGAAAGCAACCAAGAAGAAGTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTC
NM_181744.3       TTGAAAGCAACCAAGAAGAAGTCTCTGGAAGGCTTCAGGCTGCACACCGTAACCACAGTC
                  ************************************************************

HsCD00510710      AGGAAGTCTTCTGCTGTGCTGGAAATTCATGAAGAGTGGGAA---
NM_181744.3       AGGAAGTCTTCTGCTGTGCTGGAAATTCATGAAGAGTGGGAATAA
                  ******************************************   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Opsins
Reactome : Signal Transduction
Reactome : Signaling by GPCR

Protein

HPRD : 12358

Clone

Cloning Information : 611982
Original Clone ID : ccsbBroadEn_05255

Organism


TAX_ID : 9606
Species Specific ID: 221391

Genome

Chromosome : 6
Map Location : 6p12.3

Reagents

Labome : OPN5-antibody