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ACTL8 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTL8
Gene Name:  actin-like 8
Sequence             
Original Clone ID: ccsbBroadEn_09073
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC028909.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00510798

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1099nts        
Open reading frame : 1 to 1099
Reference Sequence Alignment

ATGGCTTCAAGAACCGTTATCATTGACCACGGGTCTGGCTTTTTGAAGGCTGGCACGGCC
GGATGGAATGAGCCTCAGATGGTCTTCCCGAACATCGTGAACTACCTACCGTGCAAGGAG
AACCCTGGCCCCAGCTATGCCCGTAGGCGTGTGAGCCTGGGCATCGACATTTGCCATCCT
GACACCTTTAGCTACCCCATCGAGCGGGGCCGCATCCTCAACTGGGAGGGTGTGCAGTAC
CTCTGGTCATTTGTGTTGGAGAACCACAGACGGGAGCAAGAGGTCCCCCCTGTGATCATC
ACGGAGACACCCTTGAGGGAGCCTGCGGACCGAAAGAAGATGCTGGAGATCCTGTTTGAG
TTGCTGCATGTCCCATCGGTCCTCCTGGCCGACCAGCTGCAGATGTCCCTGTATGCCTCT
GGCCTCCTGACCGGAGTGGTGGTTGATTCTGGCTATGGCCTGACCCGCGTGCAGCCTTTC
CACCAGGGCCGCCCCTTGCCCGCCAGCGGCAAGACGCTGGAGTTCGCCGGCCAGGATCTC
TCCGCCTATCTCCTCAAGAGTCTCTTTAAGGAAGATTGCGATAGACGCTGCCTGTTTCAG
CTGGAGACAGTCGCCGTGACTCAGATGAACAAGTGCTACGTGCCGCAGAATCTGGGGGAG
GCCCTGGACTTTCGTGAGAGGCAGCAGAGTGCCTTGGATGAGAGCAACACCTATCAGCTC
CCAGACGGCTCCCGCGTGGAGCTGACCCCCATGCAGCGGGTGGCTCCTGAGATGTTCTTT
AGCCCGCAGGTGTTCGAGCAGCCGGGGCCCAGCATCCCACGGGCCATTGTGGAATCCGTG
GAGTCCTGCGAGATCTCCCTGCGCCCCCTGCTGGTCTCCCACGTGATGGCCTGCGGGGGC
AACACCCTCTATCCCGGGTTCACAAAGCGCCTGTTCAGGGAGCTGATGGGGGATCACGTC
TCCTCCACCAAGGCCACAGTCTGGGAGGGTTCCAATAGAAACTTTAGTGTCTGGCTAGGA
GCGTCCGTGGTGGCTCACCTTTCTACCTACCAGTCTGAGTGGATGTCCCGAGAGGAGTAT
GGTGAGCATATGAGGATG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTL8
Symbol Nomenclature : ACTL8
SYNONYM : CT57
Designation : actin like protein|actin-like protein 8|cancer/testis antigen 57
Full Nomenclature : actin-like 8
GENEID : 81569
GenBank Accession : JF432692
HGNC : 24018
Vega : OTTHUMG00000002512
Target GenBank: JF432692

 

Reference Sequence Alignment

  

HsCD00510798      ATGGCTTCAAGAACCGTTATCATTGACCACGGGTCTGGCTTTTTGAAGGCTGGCACGGCC
NM_030812.2       ATGGCTGCAAGAACCGTTATCATTGACCACGGGTCTGGCTTTTTGAAGGCTGGCACGGCC
                  ****** *****************************************************

HsCD00510798      GGATGGAATGAGCCTCAGATGGTCTTCCCGAACATCGTGAACTACCTACCGTGCAAGGAG
NM_030812.2       GGCTGGAATGAGCCTCAGATGGTCTTCCCGAACATCGTGAACTACCTACCGTGCAAGGAG
                  **.*********************************************************

HsCD00510798      AACCCTGGCCCCAGCTATGCCCGTAGGCGTGTGAGCCTGGGCATCGACATTTGCCATCCT
NM_030812.2       AACCCTGGCCCCAGCTATGCCCGTAGGCGTGTGAGCCTGGGCATCGACATTTGCCATCCT
                  ************************************************************

HsCD00510798      GACACCTTTAGCTACCCCATCGAGCGGGGCCGCATCCTCAACTGGGAGGGTGTGCAGTAC
NM_030812.2       GACACCTTTAGCTACCCCATCGAGCGGGGCCGCATCCTCAACTGGGAGGGTGTGCAGTAC
                  ************************************************************

HsCD00510798      CTCTGGTCATTTGTGTTGGAGAACCACAGACGGGAGCAAGAGGTCCCCCCTGTGATCATC
NM_030812.2       CTCTGGTCATTTGTGTTGGAGAACCACAGACGGGAGCAAGAGGTCCCCCCTGTGATCATC
                  ************************************************************

HsCD00510798      ACGGAGACACCCTTGAGGGAGCCTGCGGACCGAAAGAAGATGCTGGAGATCCTGTTTGAG
NM_030812.2       ACGGAGACACCCTTGAGGGAGCCTGCGGACCGAAAGAAGATGCTGGAGATCCTGTTTGAG
                  ************************************************************

HsCD00510798      TTGCTGCATGTCCCATCGGTCCTCCTGGCCGACCAGCTGCAGATGTCCCTGTATGCCTCT
NM_030812.2       TTGCTGCATGTCCCATCGGTCCTCCTGGCCGACCAGCTGCAGATGTCCCTGTATGCCTCT
                  ************************************************************

HsCD00510798      GGCCTCCTGACCGGAGTGGTGGTTGATTCTGGCTATGGCCTGACCCGCGTGCAGCCTTTC
NM_030812.2       GGCCTCCTGACCGGAGTGGTGGTTGATTCTGGCTATGGCCTGACCCGCGTGCAGCCTTTC
                  ************************************************************

HsCD00510798      CACCAGGGCCGCCCCTTGCCCGCCAGCGGCAAGACGCTGGAGTTCGCCGGCCAGGATCTC
NM_030812.2       CACCAGGGCCGCCCCTTGCCCGCCAGCGGCAAGACGCTGGAGTTCGCCGGCCAGGATCTC
                  ************************************************************

HsCD00510798      TCCGCCTATCTCCTCAAGAGTCTCTTTAAGGAAGATTGCGATAGACGCTGCCTGTTTCAG
NM_030812.2       TCCGCCTATCTCCTCAAGAGTCTCTTTAAGGAAGATTGCGATAGACGCTGCCTGTTTCAG
                  ************************************************************

HsCD00510798      CTGGAGACAGTCGCCGTGACTCAGATGAACAAGTGCTACGTGCCGCAGAATCTGGGGGAG
NM_030812.2       CTGGAGACAGTCGCCGTGACTCAGATGAACAAGTGCTACGTGCCGCAGAATCTGGGGGAG
                  ************************************************************

HsCD00510798      GCCCTGGACTTTCGTGAGAGGCAGCAGAGTGCCTTGGATGAGAGCAACACCTATCAGCTC
NM_030812.2       GCCCTGGACTTTCGTGAGAGGCAGCAGAGTGCCTTGGATGAGAGCAACACCTATCAGCTC
                  ************************************************************

HsCD00510798      CCAGACGGCTCCCGCGTGGAGCTGACCCCCATGCAGCGGGTGGCTCCTGAGATGTTCTTT
NM_030812.2       CCAGACGGCTCCCGCGTGGAGCTGACCCCCATGCAGCGGGTGGCTCCTGAGATGTTCTTT
                  ************************************************************

HsCD00510798      AGCCCGCAGGTGTTCGAGCAGCCGGGGCCCAGCATCCCACGGGCCATTGTGGAATCCGTG
NM_030812.2       AGCCCGCAGGTGTTCGAGCAGCCGGGGCCCAGCATCCCACGGGCCATTGTGGAATCCGTG
                  ************************************************************

HsCD00510798      GAGTCCTGCGAGATCTCCCTGCGCCCCCTGCTGGTCTCCCACGTGATGGCCTGCGGGGGC
NM_030812.2       GAGTCCTGCGAGATCTCCCTGCGCCCCCTGCTGGTCTCCCACGTGATGGCCTGCGGGGGC
                  ************************************************************

HsCD00510798      AACACCCTCTATCCCGGGTTCACAAAGCGCCTGTTCAGGGAGCTGATGGGGGATCACGTC
NM_030812.2       AACACCCTCTATCCCGGGTTCACAAAGCGCCTGTTCAGGGAGCTGATGGGGGATCACGTC
                  ************************************************************

HsCD00510798      TCCTCCACCAAGGCCACAGTCTGGGAGGGTTCCAATAGAAACTTTAGTGTCTGGCTAGGA
NM_030812.2       TCCTCCACCAAGGCCACAGTCTGGGAGGGTTCCAATAGAAACTTTAGTGTCTGGCTAGGA
                  ************************************************************

HsCD00510798      GCGTCCGTGGTGGCTCACCTTTCTACCTACCAGTCTGAGTGGATGTCCCGAGAGGAGTAT
NM_030812.2       GCGTCCGTGGTGGCTCACCTTTCTACCTACCAGTCTGAGTGGATGTCCCGAGAGGAGTAT
                  ************************************************************

HsCD00510798      GGTGAGCATATGAGGATG---
NM_030812.2       GGTGAGCATATGAGGATGTGA
                  ******************   

Protein

SMART domain : ACTIN : Actin
HPRD : 11274

Clone

Cloning Information : 612070
Original Clone ID : ccsbBroadEn_09073

Organism


TAX_ID : 9606
Species Specific ID: 81569

Genome

Chromosome : 1
Map Location : 1p36.2-p35
Ensembl : ENSG00000117148

Reagents

Labome : ACTL8-antibody