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ACTR1A (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTR1B
Gene Name:  ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
Sequence             
Original Clone ID: ccsbBroadEn_02320
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC000693.2,BC026016.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 26322679
Title: Proteomic mapping of ER_PM junctions identifies STIMATE as a regulator of Ca2+ influx
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00510884

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1129nts        
Open reading frame : 1 to 1129
Reference Sequence Alignment

ATGGAGTCCTACGATGTGATCGCCAACCAGCCTGTCGTGATCGACAACGGATCCGGTGTG
ATTAAAGCTGGTTTTGCTGGTGATCAGATCCCCAAATACTGCTTTCCAAACTATGTGGGC
CGACCCAAGCACGTTCGTGTCATGGCAGGAGCCCTTGAAGGCGACATCTTCATTGGCCCC
AAAGCTGAGGAGCACCGAGGGCTGCTTTCAATCCGCTATCCCATGGAGCATGGCATCGTC
AAGGATTGGAACGACATGGAACGCATTTGGCAATATGTCTATTCTAAGGACCAGCTGCAG
ACTTTCTCAGAGGAGCATCCTGTGCTCCTGACTGAGGCGCCTTTAAACCCACGAAAAAAC
CGGGAACGAGCTGCCGAAGTTTTCTTCGAGACCTTCAATGTGCCCGCTCTTTTCATCTCC
ATGCAAGCTGTACTCAGCCTTTACGCTACAGGCAGGACCACAGGGGTGGTGCTGGATTCT
GGGGATGGAGTCACCCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCCCACTCCATC
ATGCGCATCGACATCGCGGGCCGGGACGTCTCTCGCTTCCTGCGCCTCTACCTGCGTAAG
GAGGGCTACGACTTCCACTCATCCTCTGAGTTTGAGATTGTCAAGGCCATAAAAGAAAGA
GCCTGTTACCTATCCATAAACCCCCAAAAGGATGAGACGCTAGAGACAGAGAAAGCTCAG
TACTACCTGCCTGATGGCAGCACCATTGAGATTGGTCCTTCCCGATTCCGGGCCCCTGAG
TTGCTCTTCAGGCCAGATTTGATTGGAGAGGAGAGTGAAGGCATCCACGAGGTCCTGGTG
TTCGCCATTCAGAAGTCAGACATGGACCTGCGGCGCACGCTTTTCTCTAACATTGTCCTC
TCAGGAGGCTCTACCCTGTTCAAAGGTTTTGGTGACAGGCTCCTGAGTGAAGTGAAGAAA
CTAGCTCCAAAAGATGTGAAGATCAGGATATCTGCACCTCAGGAGAGACTGTATTCCACG
TGGATTGGGGGCTCCATCCTTGCCTCCCTGGACACCTTTAAGAAGATGTGGGTCTCCAAA
AAGGAATATGAGGAAGACGGTGCCCGATCCATCCACAGAAAAACCTTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTR1B
Symbol Nomenclature : ACTR1B
SYNONYM : ARP1B; CTRN2; PC3
Designation : actin-related protein 1B|beta-centractin|centractin beta
Full Nomenclature : ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
GENEID : 10120
GenBank Accession : DQ895934
HGNC : 168
MIM : 605144
Vega : OTTHUMG00000130549
Target GenBank: DQ895934

 

Reference Sequence Alignment

  

HsCD00510884      ATGGAGTCCTACGATGTGATCGCCAACCAGCCTGTCGTGATCGACAACGGATCCGGTGTG
NM_005735.3       ATGGAGTCCTACGACATCATCGCCAACCAGCCTGTGGTCATCGACAACGGTTCGGGGGTG
NM_005736.3       ATGGAGTCCTACGATGTGATCGCCAACCAGCCTGTCGTGATCGACAACGGATCCGGTGTG
                  ************** .* ***************** ** ***********:** ** ***

HsCD00510884      ATTAAAGCTGGTTTTGCTGGTGATCAGATCCCCAAATACTGCTTTCCAAACTATGTGGGC
NM_005735.3       ATTAAAGCTGGCTTTGCAGGAGACCAGATTCCCAAATACTGTTTCCCAAACTATGTCGGG
NM_005736.3       ATTAAAGCTGGTTTTGCTGGTGATCAGATCCCCAAATACTGCTTTCCAAACTATGTGGGC
                  *********** *****:**:** ***** *********** ** *********** ** 

HsCD00510884      CGACCCAAGCACGTTCGTGTCATGGCAGGAGCCCTTGAAGGCGACATCTTCATTGGCCCC
NM_005735.3       CGGCCGAAGCACATGCGGGTGATGGCTGGAGCCCTGGAGGGGGACCTCTTCATCGGACCA
NM_005736.3       CGACCCAAGCACGTTCGTGTCATGGCAGGAGCCCTTGAAGGCGACATCTTCATTGGCCCC
                  **.** ******.* ** ** *****:******** **.** ***.******* **.**.

HsCD00510884      AAAGCTGAGGAGCACCGAGGGCTGCTTTCAATCCGCTATCCCATGGAGCATGGCATCGTC
NM_005735.3       AAAGCAGAGGAGCACCGGGGGCTGCTGACCATCCGCTACCCCATGGAGCACGGCGTGGTG
NM_005736.3       AAAGCTGAGGAGCACCGAGGGCTGCTTTCAATCCGCTATCCCATGGAGCATGGCATCGTC
                  *****:***********.******** :*.******** *********** ***.* ** 

HsCD00510884      AAGGATTGGAACGACATGGAACGCATTTGGCAATATGTCTATTCTAAGGACCAGCTGCAG
NM_005735.3       CGAGACTGGAACGACATGGAACGCATCTGGCAGTACGTCTACTCCAAGGATCAGCTGCAG
NM_005736.3       AAGGATTGGAACGACATGGAACGCATTTGGCAATATGTCTATTCTAAGGACCAGCTGCAG
                  ...** ******************** *****.** ***** ** ***** *********

HsCD00510884      ACTTTCTCAGAGGAGCATCCTGTGCTCCTGACTGAGGCGCCTTTAAACCCACGAAAAAAC
NM_005735.3       ACCTTCTCGGAGGAGCATCCTGTGCTCCTCACGGAGGCCCCGCTCAACCCGAGTAAGAAC
NM_005736.3       ACTTTCTCAGAGGAGCATCCTGTGCTCCTGACTGAGGCGCCTTTAAACCCACGAAAAAAC
                  ** *****.******************** ** ***** **  *.*****..*:**.***

HsCD00510884      CGGGAACGAGCTGCCGAAGTTTTCTTCGAGACCTTCAATGTGCCCGCTCTTTTCATCTCC
NM_005735.3       CGGGAGAAGGCGGCAGAGGTGTTCTTTGAGACCTTCAACGTGCCGGCCCTGTTCATCTCC
NM_005736.3       CGGGAACGAGCTGCCGAAGTTTTCTTCGAGACCTTCAATGTGCCCGCTCTTTTCATCTCC
                  *****....** **.**.** ***** *********** ***** ** ** *********

HsCD00510884      ATGCAAGCTGTACTCAGCCTTTACGCTACAGGCAGGACCACAGGGGTGGTGCTGGATTCT
NM_005735.3       ATGCAGGCTGTGCTCAGTCTGTACGCAACAGGACGCACGACAGGAGTGGTTCTAGACTCA
NM_005736.3       ATGCAAGCTGTACTCAGCCTTTACGCTACAGGCAGGACCACAGGGGTGGTGCTGGATTCT
                  *****.*****.***** ** *****:*****..* ** *****.***** **.** **:

HsCD00510884      GGGGATGGAGTCACCCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCCCACTCCATC
NM_005735.3       GGGGACGGGGTCACTCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCTCACTCCATC
NM_005736.3       GGGGATGGAGTCACCCATGCTGTGCCCATCTATGAGGGCTTTGCCATGCCCCACTCCATC
                  ***** **.***** *********************************** *********

HsCD00510884      ATGCGCATCGACATCGCGGGCCGGGACGTCTCTCGCTTCCTGCGCCTCTACCTGCGTAAG
NM_005735.3       ATGCGGGTGGACATTGCCGGCCGCGACGTCTCCCGCTACCTCCGACTCCTGCTGCGCAAG
NM_005736.3       ATGCGCATCGACATCGCGGGCCGGGACGTCTCTCGCTTCCTGCGCCTCTACCTGCGTAAG
                  ***** .* ***** ** ***** ******** ****:*** **.*** : ***** ***

HsCD00510884      GAGGGCTACGACTTCCACTCATCCTCTGAGTTTGAGATTGTCAAGGCCATAAAAGAAAGA
NM_005735.3       GAAGGGGTTGACTTCCATACCTCGGCTGAGTTTGAGGTTGTCCGGACAATCAAAGAGCGA
NM_005736.3       GAGGGCTACGACTTCCACTCATCCTCTGAGTTTGAGATTGTCAAGGCCATAAAAGAAAGA
                  **.**  : ******** :*.**  ***********.*****..*.*.**.*****..**

HsCD00510884      GCCTGTTACCTATCCATAAACCCCCAAAAGGATGAGACGCTAGAGACAGAGAAAGCTCAG
NM_005735.3       GCGTGCTACCTGTCCATCAACCCACAGAAGGATGAGGCTCTGGAGACGGAGAAGGTGCAG
NM_005736.3       GCCTGTTACCTATCCATAAACCCCCAAAAGGATGAGACGCTAGAGACAGAGAAAGCTCAG
                  ** ** *****.*****.*****.**.*********.* **.*****.*****.*  ***

HsCD00510884      TACTACCTGCCTGATGGCAGCACCATTGAGATTGGTCCTTCCCGATTCCGGGCCCCTGAG
NM_005735.3       TACACGTTGCCAGACGGCAGCACGCTTGATGTGGGGCCTGCACGATTCCGGGCCCCCGAG
NM_005736.3       TACTACCTGCCTGATGGCAGCACCATTGAGATTGGTCCTTCCCGATTCCGGGCCCCTGAG
                  ***:.  ****:** ******** .**** .* ** *** *.************** ***

HsCD00510884      TTGCTCTTCAGGCCAGATTTGATTGGAGAGGAGAGTGAAGGCATCCACGAGGTCCTGGTG
NM_005735.3       CTGCTGTTCCAGCCGGACCTTGTCGGGGATGAGAGTGAGGGGCTCCATGAGGTGGTGGCC
NM_005736.3       TTGCTCTTCAGGCCAGATTTGATTGGAGAGGAGAGTGAAGGCATCCACGAGGTCCTGGTG
                   **** ***..***.**  * .* **.** ********.** .**** *****  ***  

HsCD00510884      TTCGCCATTCAGAAGTCAGACATGGACCTGCGGCGCACGCTTTTCTCTAACATTGTCCTC
NM_005735.3       TTCGCCATACACAAGTCCGACATGGACCTGCGCCGGACGCTGTTCGCCAACATCGTGCTC
NM_005736.3       TTCGCCATTCAGAAGTCAGACATGGACCTGCGGCGCACGCTTTTCTCTAACATTGTCCTC
                  ********:** *****.************** ** ***** *** * ***** ** ***

HsCD00510884      TCAGGAGGCTCTACCCTGTTCAAAGGTTTTGGTGACAGGCTCCTGAGTGAAGTGAAGAAA
NM_005735.3       TCAGGTGGCTCAACGCTTTTCAAAGGCTTCGGAGACCGATTACTCAGTGAAGTGAAGAAG
NM_005736.3       TCAGGAGGCTCTACCCTGTTCAAAGGTTTTGGTGACAGGCTCCTGAGTGAAGTGAAGAAA
                  *****:*****:** ** ******** ** **:***.*. *.** **************.

HsCD00510884      CTAGCTCCAAAAGATGTGAAGATCAGGATATCTGCACCTCAGGAGAGACTGTATTCCACG
NM_005735.3       CTTGCCCCAAAGGATATCAAAATCAAGATCTCAGCCCCGCAGGAACGGCTGTACTCCACA
NM_005736.3       CTAGCTCCAAAAGATGTGAAGATCAGGATATCTGCACCTCAGGAGAGACTGTATTCCACG
                  **:** *****.***.* **.****.***.**:**.** *****..*.***** *****.

HsCD00510884      TGGATTGGGGGCTCCATCCTTGCCTCCCTGGACACCTTTAAGAAGATGTGGGTCTCCAAA
NM_005735.3       TGGATTGGCGGCTCCATCCTGGCCTCGCTGGACACTTTTAAGAAGATGTGGGTGTCCAAA
NM_005736.3       TGGATTGGGGGCTCCATCCTTGCCTCCCTGGACACCTTTAAGAAGATGTGGGTCTCCAAA
                  ******** *********** ***** ******** ***************** ******

HsCD00510884      AAGGAATATGAGGAAGACGGTGCCCGATCCATCCACAGAAAAACCTTC---
NM_005735.3       AAGGAGTATGAAGAGGATGGCTCCCGTGCTATTCATCGCAAAACTTTCTAG
NM_005736.3       AAGGAATATGAGGAAGACGGTGCCCGATCCATCCACAGAAAAACCTTCTAA
                  *****.*****.**.** **  ****: * ** ** .*.***** ***   

Pathways

Panther : Nicotinic acetylcholine receptor signaling pathway
Reactome : Adaptive Immune System
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Centrosome maturation
Reactome : G2/M Transition
Reactome : Immune System
Reactome : Loss of Nlp from mitotic centrosomes
Reactome : Loss of proteins required for interphase microtubule organizationÿ from the centrosome
Reactome : MHC class II antigen presentation
Reactome : Mitotic G2-G2/M phases
Reactome : Recruitment of mitotic centrosome proteins and complexes
Reactome : Regulation of PLK1 Activity at G2/M Transition

Protein

SMART domain : ACTIN : Actin
HPRD : 05508

Clone

Cloning Information : 610686
Original Clone ID : ccsbBroadEn_02320

Organism


TAX_ID : 9606
Species Specific ID: 10121

Genome

Chromosome : 2
Map Location : 2q11.1-q11.2
Ensembl : ENSG00000115073

Reagents

Labome : ACTR1A-antibody