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MTRF1L (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  MTRF1L
Gene Name:  mitochondrial translational release factor 1-like
Sequence             
Original Clone ID: ccsbBroadEn_08390
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC011873.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00510958

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1141nts         Open reading frame : 1 to 1141
Reference Sequence Alignment

ATGCGGTCCCGGGTTCTGTGGGGCGCTGCCCGGTGGCTCTGGCCCCGCCGGGCCGTTGGC
CCAGCCCGCCGGCCCCTGAGCTCCGGTAGCCCGCCGCTGGAGGAGCTGTTCGCCCGGGGC
GGGCCCTTGCGGACCTTCCTCGAGCGCCAGGCGGGGTCTGAAGCCCATTTGAAGGTCAGG
AGGCCCGAGTTGCTGGCGGTGATCAAACTGCTGAACGAGAAGGAGCAGGAGCTGCGGGAG
ACTGAGCACTTGCTGCACGATGAGAATGAAGATTTAAGGAAACTTGCAGAGAATGAAATC
ACTTTGTGTCAAAAAGAAATAACTCAGCTGAAGCATCAGATTATCTTACTTTTGGTTCCC
TCAGAAGAAACAGATGAAAATGATTTGATCCTGGAAGTAACTGCAGGAGTTGGAGGTCAG
GAGGCAATGTTGTTTACATCAGAGATATTTGATATGTATCAGCAATATGCTGCATTTAAA
AGATGGCATTTTGAAACCCTGGAATATTTTCCAAGTGAACTAGGTGGCCTTAGACATGCA
TCTGCCAGCATTGGGGGTTCAGAAGCCTATAGGCACATGAAATTTGAAGGAGGTGTTCAC
AGAGTACAAAGAGTGCCAAAGACAGAAAAGCAAGGCCGCGTCCATACTAGCACCATGACT
GTAGCAATATTACCCCAGCCTACTGAGATTAATCTGGTGATTAATCCGAAAGATTTGAGA
ATTGACACTAAGCGAGCCAGTGGAGCTGGGGGGCAGCATGTAAATACCACGGACAGTGCT
GTCCGGATAGTTCATCTTCCAACAGGTGTTGTTTCTGAATGTCAACAAGAGAGATCTCAG
CTGAAAAATAAAGAGCTGGCTATGACAAAGTTACGTGCAAAACTGTACAGCATGCATCTA
GAAGAAGAAATAAATAAAAGACAGAATGCTAGAAAAATTCAGATTGGAAGTAAAGGAAGA
TCAGAGAAAATAAGAACATATAATTTTCCACAGAACCGGGTCACAGATCACAGAATAAAC
AAGACGCTGCATGATCTTGAAACTTTTATGCAAGGAGATTATCTACTGGATGAACTTGTA
CAGTCATTGAAGGAATACGCCGATTATGAATCTTTAGTAGAAATTATTTCCCAAAAAGTT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MTRF1L
Symbol Nomenclature : MTRF1L
SYNONYM : HMRF1L; MRF1L; mtRF1a
Designation : mitochondrial release factor 1 like|peptide chain release factor 1-like, mitochondrial
Full Nomenclature : mitochondrial translational release factor 1-like
GENEID : 54516
Locus Tag : RP1-101K10.1
GenBank Accession : HQ447880
HGNC : 21051
MIM : 613542
Vega : OTTHUMG00000015857
Target GenBank: HQ447880

 

Reference Sequence Alignment

  

HsCD00510958      ATGCGGTCCCGGGTTCTGTGGGGCGCTGCCCGGTGGCTCTGGCCCCGCCGGGCCGTTGGC
NM_019041.5       ATGCGGTCCCGGGTTCTGTGGGGCGCTGCCCGGTGGCTCTGGCCCCGCCGGGCCGTTGGC
                  ************************************************************

HsCD00510958      CCAGCCCGCCGGCCCCTGAGCTCCGGTAGCCCGCCGCTGGAGGAGCTGTTCGCCCGGGGC
NM_019041.5       CCAGCCCGCCGGCCCCTGAGCTCCGGTAGCCCGCCGCTGGAGGAGCTGTTCACCCGGGGC
                  ***************************************************.********

HsCD00510958      GGGCCCTTGCGGACCTTCCTCGAGCGCCAGGCGGGGTCTGAAGCCCATTTGAAGGTCAGG
NM_019041.5       GGGCCCTTGCGGACCTTCCTCGAGCGCCAGGCGGGGTCTGAAGCCCATTTGAAGGTCAGG
                  ************************************************************

HsCD00510958      AGGCCCGAGTTGCTGGCGGTGATCAAACTGCTGAACGAGAAGGAGCAGGAGCTGCGGGAG
NM_019041.5       AGGCCCGAGTTGCTGGCGGTGATCAAACTGCTGAACGAGAAGGAGCGGGAGCTGCGGGAG
                  **********************************************.*************

HsCD00510958      ACTGAGCACTTGCTGCACGATGAGAATGAAGATTTAAGGAAACTTGCAGAGAATGAAATC
NM_019041.5       ACTGAGCACTTGCTGCACGATGAGAATGAAGATTTAAGGAAACTTGCAGAGAATGAAATC
                  ************************************************************

HsCD00510958      ACTTTGTGTCAAAAAGAAATAACTCAGCTGAAGCATCAGATTATCTTACTTTTGGTTCCC
NM_019041.5       ACTTTGTGTCAAAAAGAAATAACTCAGCTGAAGCATCAGATTATCTTACTTTTGGTTCCC
                  ************************************************************

HsCD00510958      TCAGAAGAAACAGATGAAAATGATTTGATCCTGGAAGTAACTGCAGGAGTTGGAGGTCAG
NM_019041.5       TCAGAAGAAACAGATGAAAATGATTTGATCCTGGAAGTAACTGCAGGAGTTGGAGGTCAG
                  ************************************************************

HsCD00510958      GAGGCAATGTTGTTTACATCAGAGATATTTGATATGTATCAGCAATATGCTGCATTTAAA
NM_019041.5       GAGGCAATGTTGTTTACATCAGAGATATTTGATATGTATCAGCAATATGCTGCATTTAAA
                  ************************************************************

HsCD00510958      AGATGGCATTTTGAAACCCTGGAATATTTTCCAAGTGAACTAGGTGGCCTTAGACATGCA
NM_019041.5       AGATGGCATTTTGAAACCCTGGAATATTTTCCAAGTGAACTAGGTGGCCTTAGACATGCA
                  ************************************************************

HsCD00510958      TCTGCCAGCATTGGGGGTTCAGAAGCCTATAGGCACATGAAATTTGAAGGAGGTGTTCAC
NM_019041.5       TCTGCCAGCATTGGGGGTTCAGAAGCCTATAGGCACATGAAATTTGAAGGAGGTGTTCAC
                  ************************************************************

HsCD00510958      AGAGTACAAAGAGTGCCAAAGACAGAAAAGCAAGGCCGCGTCCATACTAGCACCATGACT
NM_019041.5       AGAGTACAAAGAGTGCCAAAGACAGAAAAGCAAGGCCGCGTCCATACTAGCACCATGACT
                  ************************************************************

HsCD00510958      GTAGCAATATTACCCCAGCCTACTGAGATTAATCTGGTGATTAATCCGAAAGATTTGAGA
NM_019041.5       GTAGCAATATTACCCCAGCCTACTGAGATTAATCTGGTGATTAATCCGAAAGATTTGAGA
                  ************************************************************

HsCD00510958      ATTGACACTAAGCGAGCCAGTGGAGCTGGGGGGCAGCATGTAAATACCACGGACAGTGCT
NM_019041.5       ATTGACACTAAGCGAGCCAGTGGAGCTGGGGGGCAGCATGTAAATACCACGGACAGTGCT
                  ************************************************************

HsCD00510958      GTCCGGATAGTTCATCTTCCAACAGGTGTTGTTTCTGAATGTCAACAAGAGAGATCTCAG
NM_019041.5       GTCCGGATAGTTCATCTTCCAACAGGTGTTGTTTCTGAATGTCAACAAGAGAGATCTCAG
                  ************************************************************

HsCD00510958      CTGAAAAATAAAGAGCTGGCTATGACAAAGTTACGTGCAAAACTGTACAGCATGCATCTA
NM_019041.5       CTGAAAAATAAAGAGCTGGCTATGACAAAGTTACGTGCAAAACTGTACAGCATGCATCTA
                  ************************************************************

HsCD00510958      GAAGAAGAAATAAATAAAAGACAGAATGCTAGAAAAATTCAGATTGGAAGTAAAGGAAGA
NM_019041.5       GAAGAAGAAATAAATAAAAGACAGAATGCTAGAAAAATTCAGATTGGAAGTAAAGGAAGA
                  ************************************************************

HsCD00510958      TCAGAGAAAATAAGAACATATAATTTTCCACAGAACCGGGTCACAGATCACAGAATAAAC
NM_019041.5       TCAGAGAAAATAAGAACATATAATTTTCCACAGAACCGGGTCACAGATCACAGAATAAAC
                  ************************************************************

HsCD00510958      AAGACGCTGCATGATCTTGAAACTTTTATGCAAGGAGATTATCTACTGGATGAACTTGTA
NM_019041.5       AAGACGCTGCATGATCTTGAAACTTTTATGCAAGGAGATTATCTACTGGATGAACTTGTA
                  ************************************************************

HsCD00510958      CAGTCATTGAAGGAATACGCCGATTATGAATCTTTAGTAGAAATTATTTCCCAAAAAGTT
NM_019041.5       CAGTCATTGAAGGAATACGCCGATTATGAATCTTTAGTAGAAATTATTTCCCAAAAAGTT
                  ************************************************************

HsCD00510958      ---
NM_019041.5       TAA
                     

Protein

SMART domain : PCRF :
HPRD : 17613

Clone

Cloning Information : 610760
Original Clone ID : ccsbBroadEn_08390

Organism


TAX_ID : 9606
Species Specific ID: 54516

Genome

Chromosome : 6
Map Location : 6q25-q26
Ensembl : ENSG00000112031

Reagents

Labome : MTRF1L-antibody