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OPN3 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  OPN3
Gene Name:  opsin 3
Sequence             
Original Clone ID: ccsbBroadEn_02806
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC036773.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00511145

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1207nts         Open reading frame : 1 to 1207
Reference Sequence Alignment

ATGTACTCGGGGAACCGCAGCGGCGGCCACGGCTACTGGGACGGCGGCGGGGCCGCGGGC
GCTGAGGGGCCGGCGCCGGCGGGGACACTGAGCCCCGCGCCCCTCTTCAGCCCCGGCACC
TACGAGCGCCTGGCGCTGCTGCTGGGCTCCATTGGGCTGCTGGGCGTCGGCAACAACCTG
CTGGTGCTCGTCCTCTACTACAAGTTCCAGCGGCTCCGCACTCCCACTCACCTCCTCCTG
GTCAACATCAGCCTCAGCGACCTGCTGGTGTCCCTCTTCGGGGTCACCTTTACCTTCGTG
TCCTGCCTGAGGAACGGCTGGGTGTGGGACACCGTGGGCTGCGTGTGGGACGGGTTTAGC
GGCAGCCTCTTCGGGATTGTTTCCATTGCCACCCTAACCGTGCTGGCCTATGAACGTTAC
ATTCGCGTGGTCCATGCCAGAGTGATCAATTTTTCCTGGGCCTGGAGGGCCATTACCTAC
ATCTGGCTCTACTCACTGGCGTGGGCAGGAGCACCTCTCCTGGGATGGAACAGGTACATC
CTGGACGTACACGGACTAGGCTGCACTGTGGACTGGAAATCCAAGGATGCCAACGATTCC
TCCTTTGTGCTTTTCTTATTTCTTGGCTGCCTGGTGGTGCCCCTGGGTGTCATAGCCCAT
TGCTATGGCCATATTCTATATTCCATTCGAATGCTTCGTTGTGTGGAAGATCTTCAGACA
ATTCAAGTGATCAAGATTTTAAAATATGAAAAGAAACTGGCCAAAATGTGCTTTTTAATG
ATATTCACCTTCCTGGTCTGTTGGATGCCTTATATCGTGATCTGCTTCTTGGTGGTTAAT
GGTCATGGTCACCTGGTCACTCCAACAATATCTATTGTTTCGTACCTCTTTGCTAAATCG
AACACTGTATACAATCCAGTGATTTATGTCTTCATGATCAGAAAGTTTCGAAGATCCCTT
TTGCAGCTTCTGTGCCTCCGACTGCTGAGGTGCCAGAGGCCTGCTAAAGACCTACCAGCA
GCTGGAAGTGAAATGCAGATCAGACCCATTGTGATGTCACAGAAAGATGGGGACAGGCCA
AAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTATCATCACCAGTGATGAATCA
CTGTCAGTTGACGACAGCGACAAAACCAATGGGTCCAAAGTTGATGTAATCCAAGTTCGT
CCTTTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : OPN3
Symbol Nomenclature : OPN3
SYNONYM : ECPN
Designation : encephalopsin|opsin 3 (encephalopsin, panopsin)|opsin-3
Full Nomenclature : opsin 3
GENEID : 23596
HGNC : 14007
MIM : 606695
Vega : OTTHUMG00000039691
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00511145      ATGTACTCGGGGAACCGCAGCGGCGGCCACGGCTACTGGGACGGCGGCGGGGCCGCGGGC
NM_014322.2       ATGTACTCGGGGAACCGCAGCGGCGGCCACGGCTACTGGGACGGCGGCGGGGCCGCGGGC
                  ************************************************************

HsCD00511145      GCTGAGGGGCCGGCGCCGGCGGGGACACTGAGCCCCGCGCCCCTCTTCAGCCCCGGCACC
NM_014322.2       GCTGAGGGGCCGGCGCCGGCGGGGACACTGAGCCCCGCGCCCCTCTTCAGCCCCGGCACC
                  ************************************************************

HsCD00511145      TACGAGCGCCTGGCGCTGCTGCTGGGCTCCATTGGGCTGCTGGGCGTCGGCAACAACCTG
NM_014322.2       TACGAGCGCCTGGCGCTGCTGCTGGGCTCCATTGGGCTGCTGGGCGTCGGCAACAACCTG
                  ************************************************************

HsCD00511145      CTGGTGCTCGTCCTCTACTACAAGTTCCAGCGGCTCCGCACTCCCACTCACCTCCTCCTG
NM_014322.2       CTGGTGCTCGTCCTCTACTACAAGTTCCAGCGGCTCCGCACTCCCACTCACCTCCTCCTG
                  ************************************************************

HsCD00511145      GTCAACATCAGCCTCAGCGACCTGCTGGTGTCCCTCTTCGGGGTCACCTTTACCTTCGTG
NM_014322.2       GTCAACATCAGCCTCAGCGACCTGCTGGTGTCCCTCTTCGGGGTCACCTTTACCTTCGTG
                  ************************************************************

HsCD00511145      TCCTGCCTGAGGAACGGCTGGGTGTGGGACACCGTGGGCTGCGTGTGGGACGGGTTTAGC
NM_014322.2       TCCTGCCTGAGGAACGGCTGGGTGTGGGACACCGTGGGCTGCGTGTGGGACGGGTTTAGC
                  ************************************************************

HsCD00511145      GGCAGCCTCTTCGGGATTGTTTCCATTGCCACCCTAACCGTGCTGGCCTATGAACGTTAC
NM_014322.2       GGCAGCCTCTTCGGGATTGTTTCCATTGCCACCCTAACCGTGCTGGCCTATGAACGTTAC
                  ************************************************************

HsCD00511145      ATTCGCGTGGTCCATGCCAGAGTGATCAATTTTTCCTGGGCCTGGAGGGCCATTACCTAC
NM_014322.2       ATTCGCGTGGTCCATGCCAGAGTGATCAATTTTTCCTGGGCCTGGAGGGCCATTACCTAC
                  ************************************************************

HsCD00511145      ATCTGGCTCTACTCACTGGCGTGGGCAGGAGCACCTCTCCTGGGATGGAACAGGTACATC
NM_014322.2       ATCTGGCTCTACTCACTGGCGTGGGCAGGAGCACCTCTCCTGGGATGGAACAGGTACATC
                  ************************************************************

HsCD00511145      CTGGACGTACACGGACTAGGCTGCACTGTGGACTGGAAATCCAAGGATGCCAACGATTCC
NM_014322.2       CTGGACGTACACGGACTAGGCTGCACTGTGGACTGGAAATCCAAGGATGCCAACGATTCC
                  ************************************************************

HsCD00511145      TCCTTTGTGCTTTTCTTATTTCTTGGCTGCCTGGTGGTGCCCCTGGGTGTCATAGCCCAT
NM_014322.2       TCCTTTGTGCTTTTCTTATTTCTTGGCTGCCTGGTGGTGCCCCTGGGTGTCATAGCCCAT
                  ************************************************************

HsCD00511145      TGCTATGGCCATATTCTATATTCCATTCGAATGCTTCGTTGTGTGGAAGATCTTCAGACA
NM_014322.2       TGCTATGGCCATATTCTATATTCCATTCGAATGCTTCGTTGTGTGGAAGATCTTCAGACA
                  ************************************************************

HsCD00511145      ATTCAAGTGATCAAGATTTTAAAATATGAAAAGAAACTGGCCAAAATGTGCTTTTTAATG
NM_014322.2       ATTCAAGTGATCAAGATTTTAAAATATGAAAAGAAACTGGCCAAAATGTGCTTTTTAATG
                  ************************************************************

HsCD00511145      ATATTCACCTTCCTGGTCTGTTGGATGCCTTATATCGTGATCTGCTTCTTGGTGGTTAAT
NM_014322.2       ATATTCACCTTCCTGGTCTGTTGGATGCCTTATATCGTGATCTGCTTCTTGGTGGTTAAT
                  ************************************************************

HsCD00511145      GGTCATGGTCACCTGGTCACTCCAACAATATCTATTGTTTCGTACCTCTTTGCTAAATCG
NM_014322.2       GGTCATGGTCACCTGGTCACTCCAACAATATCTATTGTTTCGTACCTCTTTGCTAAATCG
                  ************************************************************

HsCD00511145      AACACTGTATACAATCCAGTGATTTATGTCTTCATGATCAGAAAGTTTCGAAGATCCCTT
NM_014322.2       AACACTGTATACAATCCAGTGATTTATGTCTTCATGATCAGAAAGTTTCGAAGATCCCTT
                  ************************************************************

HsCD00511145      TTGCAGCTTCTGTGCCTCCGACTGCTGAGGTGCCAGAGGCCTGCTAAAGACCTACCAGCA
NM_014322.2       TTGCAGCTTCTGTGCCTCCGACTGCTGAGGTGCCAGAGGCCTGCTAAAGACCTACCAGCA
                  ************************************************************

HsCD00511145      GCTGGAAGTGAAATGCAGATCAGACCCATTGTGATGTCACAGAAAGATGGGGACAGGCCA
NM_014322.2       GCTGGAAGTGAAATGCAGATCAGACCCATTGTGATGTCACAGAAAGATGGGGACAGGCCA
                  ************************************************************

HsCD00511145      AAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTATCATCACCAGTGATGAATCA
NM_014322.2       AAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTATCATCACCAGTGATGAATCA
                  ************************************************************

HsCD00511145      CTGTCAGTTGACGACAGCGACAAAACCAATGGGTCCAAAGTTGATGTAATCCAAGTTCGT
NM_014322.2       CTGTCAGTTGACGACAGCGACAAAACCAATGGGTCCAAAGTTGATGTAATCCAAGTTCGT
                  ************************************************************

HsCD00511145      CCTTTG---
NM_014322.2       CCTTTGTAG
                  ******   

Pathways

Reactome : Class A/1 (Rhodopsin-like receptors)
Reactome : G alpha (i) signalling events
Reactome : GPCR downstream signaling
Reactome : GPCR ligand binding
Reactome : Opsins
Reactome : Signal Transduction
Reactome : Signaling by GPCR
WikiPathway : GPCRs, Class A Rhodopsin-like

Protein

HPRD : 05985

Clone

Cloning Information : 611111
Original Clone ID : ccsbBroadEn_02806

Organism


TAX_ID : 9606
Species Specific ID: 23596

Genome

Chromosome : 1
Map Location : 1q43
Ensembl : ENSG00000054277

Reagents

Labome : OPN3-antibody