DNASU Plasmid Repository • 480.965.5697 | Email

ACTR6 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  ACTR6
Gene Name:  ARP6 actin-related protein 6 homolog (yeast)
Sequence             
Original Clone ID: ccsbBroadEn_03952
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC015107.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00511153

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1189nts        
Open reading frame : 1 to 1189
Reference Sequence Alignment

ATGACGACCTTAGTGCTGGATAATGGAGCTTACAACGCCAAAATCGGTTACAGCCATGAA
AATGTGTCGGTTATTCCTAATTGTCAGTTCCGGTCAAAAACAGCACGTCTTAAAACTTTT
ACTGCCAACCAGATAGATGAAATAAAAGACCCTTCTGGACTCTTTTACATCCTCCCTTTT
CAAAAGGGCTACTTGGTGAATTGGGATGTTCAGAGACAAGTTTGGGATTACCTTTTTGGA
AAAGAAATGTATCAGGTTGATTTTTTAGATACTAATATTATTATCACTGAACCATACTTT
AACTTCACTTCAATTCAAGAATCAATGAATGAAATTCTATTTGAAGAATACCAGTTTCAA
GCAGTATTAAGAGTAAATGCTGGGGCTCTCAGTGCACATAGGTATTTCCGAGATAATCCT
TCCGAATTATGCTGTATCATTGTTGATAGTGGATATTCCTTTACACATATAGTTCCTTAT
TGTAGAAGTAAAAAGAAAAAAGAAGCAATTATTCGGATAAATGTGGGAGGAAAACTCTTA
ACCAATCATCTAAAGGAGATCATATCTTACAGGCAGCTACATGTTATGGATGAAACACAT
GTGATTAATCAAGTGAAAGAAGATGTATGCTATGTGTCTCAGGATTTTTATAGAGACATG
GATATTGCAAAGTTGAAAGGAGAAGAAAATACAGTAATGATAGACTATGTCTTGCCTGAC
TTCAGTACAATTAAAAAGGGCTTTTGTAAGCCAAGGGAAGAGATGGTGTTGAGTGGAAAA
TACAAATCTGGGGAACAAATTCTTCGTTTGGCCAATGAGAGATTTGCTGTTCCGGAAATA
CTCTTTAATCCTTCTGATATAGGCATTCAAGAAATGGGAATTCCAGAAGCTATTGTCTAT
TCAATTCAAAATCTACCTGAAGAAATGCAGCCGCATTTTTTTAAGAACATTGTCTTGACA
GGAGGAAATTCCCTTTTCCCAGGATTTAGGGATCGGGTTTACTCAGAAGTTCGATGTCTT
ACTCCAACAGATTATGATGTTTCTGTTGTGCTGCCTGAAAACCCTATTACTTATGCCTGG
GAAGGTGGAAAATTGATATCAGAGAATGATGATTTTGAAGATATGGTGGTAACAAGAGAA
GATTACGAAGAAAATGGACATAGCGTCTGTGAAGAGAAATTTGATATT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTR6
Symbol Nomenclature : ACTR6
SYNONYM : ARP6; HSPC281; MSTP136; hARP6; hARPX
Designation : actin-related protein 6
Full Nomenclature : ARP6 actin-related protein 6 homolog (yeast)
GENEID : 64431
Locus Tag : CDA12
GenBank Accession : HQ447318
HGNC : 24025
Vega : OTTHUMG00000170245
Target GenBank: HQ447318

 

Reference Sequence Alignment

  

HsCD00511153      ATGACGACCTTAGTGCTGGATAATGGAGCTTACAACGCCAAAATCGGTTACAGCCATGAA
NM_022496.4       ATGACGACCTTAGTGCTGGATAATGGAGCTTACAACGCCAAAATCGGTTACAGCCATGAA
                  ************************************************************

HsCD00511153      AATGTGTCGGTTATTCCTAATTGTCAGTTCCGGTCAAAAACAGCACGTCTTAAAACTTTT
NM_022496.4       AATGTGTCGGTTATTCCTAATTGTCAGTTCCGGTCAAAAACAGCACGTCTTAAAACTTTT
                  ************************************************************

HsCD00511153      ACTGCCAACCAGATAGATGAAATAAAAGACCCTTCTGGACTCTTTTACATCCTCCCTTTT
NM_022496.4       ACTGCCAACCAGATAGATGAAATAAAAGACCCTTCTGGACTCTTTTACATCCTCCCTTTT
                  ************************************************************

HsCD00511153      CAAAAGGGCTACTTGGTGAATTGGGATGTTCAGAGACAAGTTTGGGATTACCTTTTTGGA
NM_022496.4       CAAAAGGGCTACTTGGTGAATTGGGATGTTCAGAGACAAGTTTGGGATTACCTTTTTGGA
                  ************************************************************

HsCD00511153      AAAGAAATGTATCAGGTTGATTTTTTAGATACTAATATTATTATCACTGAACCATACTTT
NM_022496.4       AAAGAAATGTATCAGGTTGATTTTTTAGATACTAATATTATTATCACTGAACCATACTTT
                  ************************************************************

HsCD00511153      AACTTCACTTCAATTCAAGAATCAATGAATGAAATTCTATTTGAAGAATACCAGTTTCAA
NM_022496.4       AACTTCACTTCAATTCAAGAATCAATGAATGAAATTCTATTTGAAGAATACCAGTTTCAA
                  ************************************************************

HsCD00511153      GCAGTATTAAGAGTAAATGCTGGGGCTCTCAGTGCACATAGGTATTTCCGAGATAATCCT
NM_022496.4       GCAGTATTAAGAGTAAATGCTGGGGCTCTCAGTGCACATAGGTATTTCCGAGATAATCCT
                  ************************************************************

HsCD00511153      TCCGAATTATGCTGTATCATTGTTGATAGTGGATATTCCTTTACACATATAGTTCCTTAT
NM_022496.4       TCCGAATTATGCTGTATCATTGTTGATAGTGGATATTCCTTTACACATATAGTTCCTTAT
                  ************************************************************

HsCD00511153      TGTAGAAGTAAAAAGAAAAAAGAAGCAATTATTCGGATAAATGTGGGAGGAAAACTCTTA
NM_022496.4       TGTAGAAGTAAAAAGAAAAAAGAAGCAATTATTCGGATAAATGTGGGAGGAAAACTCTTA
                  ************************************************************

HsCD00511153      ACCAATCATCTAAAGGAGATCATATCTTACAGGCAGCTACATGTTATGGATGAAACACAT
NM_022496.4       ACCAATCATCTAAAGGAGATCATATCTTACAGGCAGCTACATGTTATGGATGAAACACAT
                  ************************************************************

HsCD00511153      GTGATTAATCAAGTGAAAGAAGATGTATGCTATGTGTCTCAGGATTTTTATAGAGACATG
NM_022496.4       GTGATTAATCAAGTGAAAGAAGATGTATGCTATGTGTCTCAGGATTTTTATAGAGACATG
                  ************************************************************

HsCD00511153      GATATTGCAAAGTTGAAAGGAGAAGAAAATACAGTAATGATAGACTATGTCTTGCCTGAC
NM_022496.4       GATATTGCAAAGTTGAAAGGAGAAGAAAATACAGTAATGATAGACTATGTCTTGCCTGAC
                  ************************************************************

HsCD00511153      TTCAGTACAATTAAAAAGGGCTTTTGTAAGCCAAGGGAAGAGATGGTGTTGAGTGGAAAA
NM_022496.4       TTCAGTACAATTAAAAAGGGCTTTTGTAAGCCAAGGGAAGAGATGGTGTTGAGTGGAAAA
                  ************************************************************

HsCD00511153      TACAAATCTGGGGAACAAATTCTTCGTTTGGCCAATGAGAGATTTGCTGTTCCGGAAATA
NM_022496.4       TACAAATCTGGGGAACAAATTCTTCGTTTGGCCAATGAGAGATTTGCTGTTCCGGAAATA
                  ************************************************************

HsCD00511153      CTCTTTAATCCTTCTGATATAGGCATTCAAGAAATGGGAATTCCAGAAGCTATTGTCTAT
NM_022496.4       CTCTTTAATCCTTCTGATATAGGCATTCAAGAAATGGGAATTCCAGAAGCTATTGTCTAT
                  ************************************************************

HsCD00511153      TCAATTCAAAATCTACCTGAAGAAATGCAGCCGCATTTTTTTAAGAACATTGTCTTGACA
NM_022496.4       TCAATTCAAAATCTACCTGAAGAAATGCAGCCGCATTTTTTTAAGAACATTGTCTTGACA
                  ************************************************************

HsCD00511153      GGAGGAAATTCCCTTTTCCCAGGATTTAGGGATCGGGTTTACTCAGAAGTTCGATGTCTT
NM_022496.4       GGAGGAAATTCCCTTTTCCCAGGATTTAGGGATCGGGTTTACTCAGAAGTTCGATGTCTT
                  ************************************************************

HsCD00511153      ACTCCAACAGATTATGATGTTTCTGTTGTGCTGCCTGAAAACCCTATTACTTATGCCTGG
NM_022496.4       ACTCCAACAGATTATGATGTTTCTGTTGTGCTGCCTGAAAACCCTATTACTTATGCCTGG
                  ************************************************************

HsCD00511153      GAAGGTGGAAAATTGATATCAGAGAATGATGATTTTGAAGATATGGTGGTAACAAGAGAA
NM_022496.4       GAAGGTGGAAAATTGATATCAGAGAATGATGATTTTGAAGATATGGTGGTAACAAGAGAA
                  ************************************************************

HsCD00511153      GATTACGAAGAAAATGGACATAGCGTCTGTGAAGAGAAATTTGATATT---
NM_022496.4       GATTACGAAGAAAATGGACATAGCGTCTGTGAAGAGAAATTTGATATTTAA
                  ************************************************   

Protein

SMART domain : ACTIN : Actin
HPRD : 12421

Clone

Cloning Information : 611119
Original Clone ID : ccsbBroadEn_03952

Organism


TAX_ID : 9606
Species Specific ID: 64431

Genome

Chromosome : 12
Map Location : 12q23.1
Ensembl : ENSG00000075089

Reagents

Labome : ACTR6-antibody