DNASU Plasmid Repository • 480.965.5697 | Email

ACTR3B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  ACTR3
Gene Name:  ARP3 actin-related protein 3 homolog (yeast)
Sequence             
Original Clone ID: ccsbBroadEn_03803
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC008682.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00511366

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1255nts        
Open reading frame : 1 to 1255
Reference Sequence Alignment

ATGGCAGGCTCCCTGCCTCCCTGCGTGGTGGACTGTGGCACCGGGTATACCAAGCTTGGC
TACGCAGGCAACACTGAGCCCCAGTTCATTATTCCTTCATGTATTGCCATCAGAGAGTCA
GCAAAGGTAGTTGACCAAGCTCAAAGGAGAGTGTTGAGGGGAGTTGATGACCTTGACTTT
TTCATAGGAGATGAAGCCATCGATAAACCTACATATGCTACAAAGTGGCCGATACGACAT
GGAATCATTGAAGACTGGGATCTTATGGAAAGGTTCATGGAGCAAGTGGTTTTTAAATAT
CTTCGAGCTGAACCTGAGGACCATTATTTTTTAATGACAGAACCTCCACTCAATACACCA
GAAAACAGAGAGTATCTTGCAGAAATTATGTTTGAATCATTTAACGTACCAGGACTCTAC
ATTGCAGTTCAGGCAGTGCTGGCCTTGGCGGCATCTTGGACATCTCGACAAGTGGGTGAA
CGTACGTTAACGGGGATAGTCATTGACAGCGGAGATGGAGTCACCCATGTTATCCCAGTG
GCAGAAGGTTATGTAATTGGAAGCTGCATCAAACACATCCCGATTGCAGGTAGAGATATT
ACGTATTTCATTCAACAGCTGCTAAGGGAGAGGGAGGTGGGAATCCCTCCTGAGCAGTCA
CTGGAGACCGCAAAAGCCATTAAGGAGAAATACTGTTACATTTGCCCCGATATAGTCAAG
GAATTTGCCAAGTATGATGTGGATCCCCGGAAGTGGATCAAACAGTACACGGGTATCAAT
GCGATCAACCAGAAGAAGTTTGTTATAGACGTTGGTTACGAAAGATTCCTGGGACCTGAA
ATATTCTTTCACCCGGAGTTTGCCAACCCAGACTTTATGGAGTCCATCTCAGATGTTGTT
GATGAAGTAATACAGAACTGCCCCATCGATGTGCGGCGCCCGCTGTATAAGAATGTCGTA
CTCTCAGGAGGCTCCACCATGTTCAGGGATTTCGGACGCCGACTGCAGAGGGATTTGAAG
AGAGTGGTGGATGCTAGGCTGAGGCTCAGCGAGGAGCTCAGCGGCGGGAGGATCAAGCCG
AAGCCTGTGGAGGTCCAGGTGGTCACGCATCACATGCAGCGCTACGCCGTGTGGTTCGGA
GGCTCCATGCTGGCCTCGACTCCCGAGTTCTTTCAGGTCTGCCACACCAAGAAGGACTAT
GAAGAGTACGGGCCCAGCATCTGCCGCCACAACCCCGTCTTTGGAGTCATGTCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTR3
Symbol Nomenclature : ACTR3
SYNONYM : ARP3
Designation : actin-like protein 3|actin-related protein 3
Full Nomenclature : ARP3 actin-related protein 3 homolog (yeast)
GENEID : 10096
GenBank Accession : EU176295
HGNC : 170
MIM : 604222
Vega : OTTHUMG00000153497
Target GenBank: EU176295

 

Reference Sequence Alignment

  

HsCD00511366      ATGGCAGGCTCCCTGCCTCCCTGCGTGGTGGACTGTGGCACCGGGTATACCAAGCTTGGC
NM_005721.4       ATGGCGGGACGGCTGCCGGCCTGTGTGGTGGACTGTGGCACGGGGTATACAAAACTAGGA
NM_020445.5       ATGGCAGGCTCCCTGCCTCCCTGCGTGGTGGACTGTGGCACCGGGTATACCAAGCTTGGC
                  *****.**.   *****  **** ***************** ********.**.**:**.

HsCD00511366      TACGCAGGCAACACTGAGCCCCAGTTCATTATTCCTTCATGTATTGCCATCAGAGAGTCA
NM_005721.4       TATGCTGGAAATACAGAACCACAGTTTATCATCCCTTCCTGTATTGCTATTAAGGAGTCA
NM_020445.5       TACGCAGGCAACACTGAGCCCCAGTTCATTATTCCTTCATGTATTGCCATCAGAGAGTCA
                  ** **:**.** **:**.**.***** ** ** *****.******** ** *..******

HsCD00511366      GCAAAGGTAGTTGACCAAGCTCAAAGGAGAGTGTTGAGGGGAGTTGATGACCTTGACTTT
NM_005721.4       GCAAAAGTGGGTGATCAAGCTCAAAGGAGGGTGATGAAAGGTGTTGATGACCTAGACTTC
NM_020445.5       GCAAAGGTAGTTGACCAAGCTCAAAGGAGAGTGTTGAGGGGAGTTGATGACCTTGACTTT
                  *****.**.* *** **************.***:***..**:***********:***** 

HsCD00511366      TTCATAGGAGATGAAGCCATCGATAAACCTACATATGCTACAAAGTGGCCGATACGACAT
NM_005721.4       TTCATTGGTGATGAAGCAATAGAAAAACCTACATATGCAACAAAGTGGCCAATCCGCCAT
NM_020445.5       TTCATAGGAGATGAAGCCATCGATAAACCTACATATGCTACAAAGTGGCCGATACGACAT
                  *****:**:********.**.**:**************:***********.**.**.***

HsCD00511366      GGAATCATTGAAGACTGGGATCTTATGGAAAGGTTCATGGAGCAAGTGGTTTTTAAATAT
NM_005721.4       GGTATAGTTGAAGATTGGGACTTAATGGAAAGGTTTATGGAGCAAGTGATCTTTAAATAT
NM_020445.5       GGAATCATTGAAGACTGGGATCTTATGGAAAGGTTCATGGAGCAAGTGGTTTTTAAATAT
                  **:**..******* *****  *:*********** ************.* *********

HsCD00511366      CTTCGAGCTGAACCTGAGGACCATTATTTTTTAATGACAGAACCTCCACTCAATACACCA
NM_005721.4       TTAAGGGCAGAACCTGAAGACCATTATTTTCTTTTGACTGAACCTCCATTGAATACTCCA
NM_020445.5       CTTCGAGCTGAACCTGAGGACCATTATTTTTTAATGACAGAACCTCCACTCAATACACCA
                   *:.*.**:********.************ *::****:********* * *****:***

HsCD00511366      GAAAACAGAGAGTATCTTGCAGAAATTATGTTTGAATCATTTAACGTACCAGGACTCTAC
NM_005721.4       GAAAACAGGGAATATACTGCTGAAATAATGTTTGAGTCCTTCAATGTTCCAGGCTTGTAC
NM_020445.5       GAAAACAGAGAGTATCTTGCAGAAATTATGTTTGAATCATTTAACGTACCAGGACTCTAC
                  ********.**.***. ***:*****:********.**.** ** **:*****. * ***

HsCD00511366      ATTGCAGTTCAGGCAGTGCTGGCCTTGGCGGCATCTTGGACATCTCGACAAGTGGGTGAA
NM_005721.4       ATTGCTGTGCAGGCTGTTCTTGCCTTAGCTGCATCTTGGACCTCAAGACAAGTAGGAGAA
NM_020445.5       ATTGCAGTTCAGGCAGTGCTGGCCTTGGCGGCATCTTGGACATCTCGACAAGTGGGTGAA
                  *****:** *****:** ** *****.** ***********.**:.*******.**:***

HsCD00511366      CGTACGTTAACGGGGATAGTCATTGACAGCGGAGATGGAGTCACCCATGTTATCCCAGTG
NM_005721.4       CGGACGTTGACCGGTACGGTAATAGACAGTGGAGATGGTGTCACTCATGTCATTCCTGTG
NM_020445.5       CGTACGTTAACGGGGATAGTCATTGACAGCGGAGATGGAGTCACCCATGTTATCCCAGTG
                  ** *****.** ** * .**.**:***** ********:***** ***** ** **:***

HsCD00511366      GCAGAAGGTTATGTAATTGGAAGCTGCATCAAACACATCCCGATTGCAGGTAGAGATATT
NM_005721.4       GCTGAAGGGTATGTGATTGGCAGCTGTATTAAACACATTCCAATCGCAGGACGAGATATA
NM_020445.5       GCAGAAGGTTATGTAATTGGAAGCTGCATCAAACACATCCCGATTGCAGGTAGAGATATT
                  **:***** *****.*****.***** ** ******** **.** *****:.*******:

HsCD00511366      ACGTATTTCATTCAACAGCTGCTAAGGGAGAGGGAGGTGGGAATCCCTCCTGAGCAGTCA
NM_005721.4       ACATATTTTATTCAGCAACTGCTGAGAGACCGAGAAGTAGGAATCCCTCCAGAACAATCC
NM_020445.5       ACGTATTTCATTCAACAGCTGCTAAGGGAGAGGGAGGTGGGAATCCCTCCTGAGCAGTCA
                  **.***** *****.**.*****.**.** .*.**.**.***********:**.**.**.

HsCD00511366      CTGGAGACCGCAAAAGCCATTAAGGAGAAATACTGTTACATTTGCCCCGATATAGTCAAG
NM_005721.4       TTGGAAACTGCTAAGGCAGTAAAGGAGCGCTATAGTTATGTCTGCCCAGATTTAGTAAAA
NM_020445.5       CTGGAGACCGCAAAAGCCATTAAGGAGAAATACTGTTACATTTGCCCCGATATAGTCAAG
                   ****.** **:**.**..*:******...** :**** .* *****.***:****.**.

HsCD00511366      GAATTTGCCAAGTATGATGTGGATCCCCGGAAGTGGATCAAACAGTACACGGGTATCAAT
NM_005721.4       GAATTTAACAAGTATGATACAGATGGGTCAAAATGGATTAAACAGTATACTGGAATCAAT
NM_020445.5       GAATTTGCCAAGTATGATGTGGATCCCCGGAAGTGGATCAAACAGTACACGGGTATCAAT
                  ******..**********. .***     .**.***** ******** ** **:******

HsCD00511366      GCGATCAACCAGAAGAAGTTTGTTATAGACGTTGGTTACGAAAGATTCCTGGGACCTGAA
NM_005721.4       GCTATCTCAAAGAAAGAGTTTTCTATCGATGTTGGTTATGAGAGATTTTTGGGACCTGAA
NM_020445.5       GCGATCAACCAGAAGAAGTTTGTTATAGACGTTGGTTACGAAAGATTCCTGGGACCTGAA
                  ** ***:...****..*****  ***.** ******** **.*****  ***********

HsCD00511366      ATATTCTTTCACCCGGAGTTTGCCAACCCAGACTTTATGGAGTCCATCTCAGATGTTGTT
NM_005721.4       ATCTTTTTTCATCCAGAGTTTGCTAATCCAGACTTTACACAACCTATCTCAGAAGTTGTA
NM_020445.5       ATATTCTTTCACCCGGAGTTTGCCAACCCAGACTTTATGGAGTCCATCTCAGATGTTGTT
                  **.** ***** **.******** ** ********** . *. * ********:*****:

HsCD00511366      GATGAAGTAATACAGAACTGCCCCATCGATGTGCGGCGCCCGCTGTATAAGAATGTCGTA
NM_005721.4       GATGAAGTAATTCAGAATTGTCCTATTGATGTCAGACGTCCTCTCTACAAGAATATTGTC
NM_020445.5       GATGAAGTAATACAGAACTGCCCCATCGATGTGCGGCGCCCGCTGTATAAGAATGTCGTA
                  ***********:***** ** ** ** ***** .*.** ** ** ** ******.* **.

HsCD00511366      CTCTCAGGAGGCTCCACCATGTTCAGGGATTTCGGACGCCGACTGCAGAGGGATTTGAAG
NM_005721.4       CTCTCTGGAGGTTCAACCATGTTCAGGGACTTTGGACGTCGCTTGCAAAGAGATTTGAAA
NM_020445.5       CTCTCAGGAGGCTCCACCATGTTCAGGGATTTCGGACGCCGACTGCAGAGGGATTTGAAG
                  *****:***** **.************** ** ***** **. ****.**.********.

HsCD00511366      AGAGTGGTGGATGCTAGGCTGAGGCTCAGCGAGGAGCTCAGCGGCGGGAGGATCAAGCCG
NM_005721.4       AGAACTGTAGATGCCCGGCTGAAATTAAGTGAGGAATTGAGTGGTGGTAGATTGAAGCCA
NM_020445.5       AGAGTGGTGGATGCTAGGCTGAGGCTCAGCGAGGAGCTCAGCGGCGGGAGGATCAAGCCG
                  ***.  **.***** .******.. *.** *****. * ** ** ** **.:* *****.

HsCD00511366      AAGCCTGTGGAGGTCCAGGTGGTCACGCATCACATGCAGCGCTACGCCGTGTGGTTCGGA
NM_005721.4       AAACCTATTGATGTACAAGTCATTACACACCACATGCAGCGATATGCAGTTTGGTTTGGA
NM_020445.5       AAGCCTGTGGAGGTCCAGGTGGTCACGCATCACATGCAGCGCTACGCCGTGTGGTTCGGA
                  **.***.* ** **.**.** .* **.** ***********.** **.** ***** ***

HsCD00511366      GGCTCCATGCTGGCCTCGACTCCCGAGTTCTTTCAGGTCTGCCACACCAAGAAGGACTAT
NM_005721.4       GGATCAATGCTGGCTTCCACGCCTGAGTTCTACCAAGTATGCCACACCAAAAAGGATTAT
NM_020445.5       GGCTCCATGCTGGCCTCGACTCCCGAGTTCTTTCAGGTCTGCCACACCAAGAAGGACTAT
                  **.**.******** ** ** ** *******: **.**.***********.***** ***

HsCD00511366      GAAGAGTACGGGCCCAGCATCTGCCGCCACAACCCCGTCTTTGGAGTCATGTCC---
NM_005721.4       GAAGAAATTGGACCTAGCATTTGTCGTCACAATCCAGTGTTTGGAGTCATGTCGTAA
NM_020445.5       GAAGAGTACGGGCCCAGCATCTGCCGCCACAACCCCGTCTTTGGAGTCATGTCCTAG
                  *****.:: **.** ***** ** ** ***** **.** **************    

Protein

SMART domain : ACTIN : Actin
HPRD : 05024

Clone

Cloning Information : 611520
Original Clone ID : ccsbBroadEn_03803

Organism


TAX_ID : 9606
Species Specific ID: 57180

Genome

Chromosome : 2
Map Location : 2q14.1
Ensembl : ENSG00000115091

Reagents

Labome : ACTR3B-antibody