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ACTL6B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTL6B
Gene Name:  actin-like 6B
Sequence             
Original Clone ID: ccsbBroadEn_03302
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC020944.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00511443

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1279nts        
Open reading frame : 1 to 1279
Reference Sequence Alignment

ATGAGCGGGGGCGTCTACGGCGGAGATGAGGTGGGGGCGCTGGTCTTTGACATTGGCTCC
TTCTCAGTCCGCGCTGGGTACGCTGGGGAGGACTGTCCCAAGGCTGACTTCCCCACCACA
GTGGGGCTGCTGGCCGCGGAGGAGGGGGGCGGGCTGGAGCTGGAGGGGGACAAAGAGAAG
AAAGGGAAGATCTTCCACATCGACACCAATGCCCTGCACGTGCCTCGGGATGGAGCGGAG
GTCATGTCGCCCCTCAAGAATGGCATGATCGAGGACTGGGAGTGCTTCCGAGCCATCCTG
GATCACACCTACAGCAAACACGTCAAGTCTGAGCCAAACCTGCACCCAGTGCTCATGTCC
GAGGCTCCGTGGAACACACGGGCCAAGCGGGAGAAGCTGACAGAGCTGATGTTCGAGCAG
TACAACATTCCTGCCTTCTTCTTATGCAAGACGGCTGTGCTCACCGCCTTTGCAAACGGG
CGGTCCACTGGCCTCGTGCTGGACAGTGGAGCCACCCACACCACGGCCATTCCAGTACAT
GACGGCTACGTTCTGCAGCAAGGCATCGTCAAGTCCCCTCTGGCAGGGGACTTCATCTCC
ATGCAGTGCCGGGAGCTGTTCCAGGAGATGGCCATTGACATCATCCCACCTTACATGATC
GCAGCCAAGGAGCCTGTCCGGGAGGGTGCCCCCCCAAACTGGAAGAAGAAGGAGAAGCTA
CCCCAGGTCTCCAAGTCCTGGCATAACTACATGTGTAATGAGGTGATCCAGGACTTCCAG
GCCTCCGTGCTGCAGGTCTCAGACTCCCCCTACGATGAACAGGTGGCTGCACAAATGCCC
ACAGTGCACTACGAGATGCCCAATGGCTACAATACAGACTACGGCGCCGAGCGACTCCGC
ATCCCTGAGGGCCTGTTTGATCCCTCGAACGTCAAGGGCCTGTCGGGGAACACCATGTTG
GGTGTGGGCCACGTGGTGACCACCAGCATCGGCATGTGTGACATTGATATTCGCCCGGGC
CTGTACGGGAGTGTCATTGTCACCGGCGGGAACACACTGCTGCAGGGCTTCACTGACAGG
CTCAATCGAGAGCTTTCCCAGAAGACCCCACCGAGCATGCGACTGAAACTCATTGCCAGC
AACAGCACCATGGAGCGCAAGTTCAGCCCCTGGATCGGGGGTTCCATCCTGGCCTCACTG
GGCACTTTCCAGCAGATGTGGATCTCCAAGCAGGAATATGAGGAGGGCGGGAAGCAGTGC
GTGGAGCGAAAGTGCCCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTL6B
Symbol Nomenclature : ACTL6B
SYNONYM : ACTL6; BAF53B
Designation : 53 kDa BRG1-associated factor B|BRG1-associated factor 53B|actin-like 6|actin-like protein 6B|actin-related protein Baf53b|arpNalpha|hArpN alpha
Full Nomenclature : actin-like 6B
GENEID : 51412
GenBank Accession : HQ447891
HGNC : 160
MIM : 612458
Vega : OTTHUMG00000159661
Target GenBank: HQ447891

 

Reference Sequence Alignment

  

HsCD00511443      ATGAGCGGGGGCGTCTACGGCGGAGATGAGGTGGGGGCGCTGGTCTTTGACATTGGCTCC
NM_016188.4       ATGAGCGGGGGCGTCTACGGCGGAGATGAGGTGGGGGCGCTGGTCTTTGACATTGGCTCC
                  ************************************************************

HsCD00511443      TTCTCAGTCCGCGCTGGGTACGCTGGGGAGGACTGTCCCAAGGCTGACTTCCCCACCACA
NM_016188.4       TTCTCAGTCCGCGCTGGGTACGCTGGGGAGGACTGTCCCAAGGCTGACTTCCCCACCACA
                  ************************************************************

HsCD00511443      GTGGGGCTGCTGGCCGCGGAGGAGGGGGGCGGGCTGGAGCTGGAGGGGGACAAAGAGAAG
NM_016188.4       GTGGGGCTGCTGGCCGCGGAGGAGGGGGGCGGGCTGGAGCTGGAGGGGGACAAAGAGAAG
                  ************************************************************

HsCD00511443      AAAGGGAAGATCTTCCACATCGACACCAATGCCCTGCACGTGCCTCGGGATGGAGCGGAG
NM_016188.4       AAAGGGAAGATCTTCCACATCGACACCAATGCCCTGCACGTGCCTCGGGATGGAGCGGAG
                  ************************************************************

HsCD00511443      GTCATGTCGCCCCTCAAGAATGGCATGATCGAGGACTGGGAGTGCTTCCGAGCCATCCTG
NM_016188.4       GTCATGTCGCCCCTCAAGAATGGCATGATCGAGGACTGGGAGTGCTTCCGAGCCATCCTG
                  ************************************************************

HsCD00511443      GATCACACCTACAGCAAACACGTCAAGTCTGAGCCAAACCTGCACCCAGTGCTCATGTCC
NM_016188.4       GATCACACCTACAGCAAACACGTCAAGTCTGAGCCAAACCTGCACCCAGTGCTCATGTCC
                  ************************************************************

HsCD00511443      GAGGCTCCGTGGAACACACGGGCCAAGCGGGAGAAGCTGACAGAGCTGATGTTCGAGCAG
NM_016188.4       GAGGCTCCGTGGAACACACGGGCCAAGCGGGAGAAGCTGACAGAGCTGATGTTCGAGCAG
                  ************************************************************

HsCD00511443      TACAACATTCCTGCCTTCTTCTTATGCAAGACGGCTGTGCTCACCGCCTTTGCAAACGGG
NM_016188.4       TACAACATTCCTGCCTTCTTCTTATGCAAGACGGCTGTGCTCACCGCCTTTGCAAACGGG
                  ************************************************************

HsCD00511443      CGGTCCACTGGCCTCGTGCTGGACAGTGGAGCCACCCACACCACGGCCATTCCAGTACAT
NM_016188.4       CGGTCCACTGGCCTCGTGCTGGACAGTGGAGCCACCCACACCACGGCCATTCCAGTACAT
                  ************************************************************

HsCD00511443      GACGGCTACGTTCTGCAGCAAGGCATCGTCAAGTCCCCTCTGGCAGGGGACTTCATCTCC
NM_016188.4       GACGGCTACGTTCTGCAGCAAGGCATCGTCAAGTCCCCTCTGGCAGGGGACTTCATCTCC
                  ************************************************************

HsCD00511443      ATGCAGTGCCGGGAGCTGTTCCAGGAGATGGCCATTGACATCATCCCACCTTACATGATC
NM_016188.4       ATGCAGTGCCGGGAGCTGTTCCAGGAGATGGCCATTGACATCATCCCACCTTACATGATC
                  ************************************************************

HsCD00511443      GCAGCCAAGGAGCCTGTCCGGGAGGGTGCCCCCCCAAACTGGAAGAAGAAGGAGAAGCTA
NM_016188.4       GCAGCCAAGGAGCCTGTCCGGGAGGGTGCCCCCCCAAACTGGAAGAAGAAGGAGAAGCTA
                  ************************************************************

HsCD00511443      CCCCAGGTCTCCAAGTCCTGGCATAACTACATGTGTAATGAGGTGATCCAGGACTTCCAG
NM_016188.4       CCCCAGGTCTCCAAGTCCTGGCATAACTACATGTGTAATGAGGTGATCCAGGACTTCCAG
                  ************************************************************

HsCD00511443      GCCTCCGTGCTGCAGGTCTCAGACTCCCCCTACGATGAACAGGTGGCTGCACAAATGCCC
NM_016188.4       GCCTCCGTGCTGCAGGTCTCAGACTCCCCCTACGATGAACAGGTGGCTGCACAAATGCCC
                  ************************************************************

HsCD00511443      ACAGTGCACTACGAGATGCCCAATGGCTACAATACAGACTACGGCGCCGAGCGACTCCGC
NM_016188.4       ACAGTGCACTACGAGATGCCCAATGGCTACAATACAGACTACGGCGCCGAGCGACTCCGC
                  ************************************************************

HsCD00511443      ATCCCTGAGGGCCTGTTTGATCCCTCGAACGTCAAGGGCCTGTCGGGGAACACCATGTTG
NM_016188.4       ATCCCTGAGGGCCTGTTTGATCCCTCGAACGTCAAGGGCCTGTCGGGGAACACCATGTTG
                  ************************************************************

HsCD00511443      GGTGTGGGCCACGTGGTGACCACCAGCATCGGCATGTGTGACATTGATATTCGCCCGGGC
NM_016188.4       GGTGTGGGCCACGTGGTGACCACCAGCATCGGCATGTGTGACATTGATATTCGCCCGGGC
                  ************************************************************

HsCD00511443      CTGTACGGGAGTGTCATTGTCACCGGCGGGAACACACTGCTGCAGGGCTTCACTGACAGG
NM_016188.4       CTGTACGGGAGTGTCATTGTCACCGGCGGGAACACACTGCTGCAGGGCTTCACTGACAGG
                  ************************************************************

HsCD00511443      CTCAATCGAGAGCTTTCCCAGAAGACCCCACCGAGCATGCGACTGAAACTCATTGCCAGC
NM_016188.4       CTCAATCGAGAGCTTTCCCAGAAGACCCCACCGAGCATGCGACTGAAACTCATTGCCAGC
                  ************************************************************

HsCD00511443      AACAGCACCATGGAGCGCAAGTTCAGCCCCTGGATCGGGGGTTCCATCCTGGCCTCACTG
NM_016188.4       AACAGCACCATGGAGCGCAAGTTCAGCCCCTGGATCGGGGGTTCCATCCTGGCCTCACTG
                  ************************************************************

HsCD00511443      GGCACTTTCCAGCAGATGTGGATCTCCAAGCAGGAATATGAGGAGGGCGGGAAGCAGTGC
NM_016188.4       GGCACTTTCCAGCAGATGTGGATCTCCAAGCAGGAATATGAGGAGGGCGGGAAGCAGTGC
                  ************************************************************

HsCD00511443      GTGGAGCGAAAGTGCCCC---
NM_016188.4       GTGGAGCGAAAGTGCCCCTGA
                  ******************   

Protein

SMART domain : ACTIN : Actin
HPRD : 12419

Clone

Cloning Information : 611690
Original Clone ID : ccsbBroadEn_03302

Organism


TAX_ID : 9606
Species Specific ID: 51412

Genome

Chromosome : 7
Map Location : 7q22
Ensembl : ENSG00000077080

Reagents

Labome : ACTL6B-antibody