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CYP3A43 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CYP3A43
Gene Name:  cytochrome P450, family 3, subfamily A, polypeptide 43
Sequence             
Original Clone ID: ccsbBroadEn_12497
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC100981.3
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00511709

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1180nts         Open reading frame : 1 to 1180
Reference Sequence Alignment

ATGGATCTCATTCCAAACTTTGCCATGGAAACATGGGTTCTTGTGGCTACCAGCCTGGTA
CTCCTCTATATTTATGGGACCCATTCACATAAACTTTTTAAGAAGCTGGGAATTCCTGGG
CCAACCCCTCTGCCTTTTCTGGGAACTATTTTGTTCTACCTTAGGAGGTCTCTGAATAAG
ATTCCCAGCTGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAAGCA
GGAGGATCACCTAAGGTCAGGAGTTCGAGACCAGCTTTGCCAACATGGGTCTTTGGAATT
TTGACAGAGAATGTAATGAAAAATACGGAGAAATGTGGGGCACTCTTTCCATTTCTTACC
CCAGTTTTTGAAGCCCTAAATATCGGTTTGTTTCCAAAAGATGTTACCCATTTTTTAAAA
AATTCCATTGAAAGGATGAAAGAAAGTCGCCTCAAAGATAAACAAAAGCATCGAGTAGAT
TTCTTTCAACAGATGATCGACTCCCAGAATTCCAAAGAAACAAAGTCCCATAAAGCTCTG
TCTGATCTGGAGCTTGTGGCCCAGTCAATTATCATCATTTTTGCTGCCTATGACACAACT
AGCACCACTCTCCCCTTCATTATGTATGAACTGGCCACTCACCCTGATGTCCAGCAGAAA
CTGCAGGAGGAGATTGACGCAGTTTTACCCAATAAGGCACCTGTCACCTACGATGCCCTG
GTACAGATGGAGTACCTTGACATGGTGGTGAATGAAACGCTCAGATTATTCCCAGTTGTT
AGTAGAGTTACGAGAGTCTGCAAGAAAGATATTGAAATCAATGGAGTGTTCATTCCCAAA
GGGTTAGCAGTGATGGTTCCAATCTATGCTCTTCACCATGACCCAAAGTACTGGACAGAG
CCTGAGAAGTTCTGCCCTGAAAGGTTCAGTAAGAAGAACAAGGACAGCATAGATCTTTAC
AGATACATACCTTTTGGAGCTGGACCCCGAAACTGCATTGGCATGAGGTTTGCTCTCACA
AACATAAAACTTGCTGTCATTAGAGCACTGCAGAACTTCTCCTTCAAACCTTGTAAAGAG
ACTCAGATCCCACTGAAATTAGACAATCTACCAATTCTTCAACCAGAAAAACCTATTGTT
CTAAAAGTGCACTTAAGAGATGGGATTACAAGTGGACCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CYP3A43
Symbol Nomenclature : CYP3A43
Designation : cytochrome P450 3A43|cytochrome P450, subfamily IIIA, polypeptide 43
Full Nomenclature : cytochrome P450, family 3, subfamily A, polypeptide 43
GENEID : 64816
HGNC : 17450
MIM : 606534
Vega : OTTHUMG00000156498
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00511709        ATGGATCTCATTCCAAACTTTGCCATGGAAACATGGGTTCTTGTGGCTACCAGCCTGGTA
NM_022820.4         ATGGATCTCATTCCAAACTTTGCCATGGAAACATGGGTTCTTGTGGCTACCAGCCTGGTA
NM_001278921.1      ATGGATCTCATTCCAAACTTTGCCATGGAAACATGGGTTCTTGTGGCTACCAGCCTGGTA
NM_057095.2         ATGGATCTCATTCCAAACTTTGCCATGGAAACATGGGTTCTTGTGGCTACCAGCCTGGTA
                    ************************************************************

HsCD00511709        CTCCTCTATATTTATGGGACCCATTCACATAAACTTTTTAAGAAGCTGGGAATTCCTGGG
NM_022820.4         CTCCTCTATATTTATGGGACCCATTCACATAAACTTTTTAAGAAGCTGGGAATTCCTGGG
NM_001278921.1      CTCCTCTATATTTATGGGACCCATTCACATAAACTTTTTAAGAAGCTGGGAATTCCTGGG
NM_057095.2         CTCCTCTATATTTATGGGACCCATTCACATAAACTTTTTAAGAAGCTGGGAATTCCTGGG
                    ************************************************************

HsCD00511709        CCAACCCCTCTGCCTTTTCTGGGAACTATTTTGTTCTACCTTAGGAGGTCTCTGAATAAG
NM_022820.4         CCAACCCCTCTGCCTTTTCTGGGAACTATTTTGTTCTACCTTAGGGGTCTTTGGAATTTT
NM_001278921.1      CCAACCCCTCTGCCTTTTCTGGGAACTATTTTGTTCTACCTTAGGAGGTCTCTGAATAAG
NM_057095.2         CCAACCCCTCTGCCTTTTCTGGGAACTATTTTGTTCTACCTTAGGGGTCTTTGGAATTTT
                    *********************************************.*   *  ****:: 

HsCD00511709        ------------------------------------------------------------
NM_022820.4         GACAGAGAATGTAATGAAAAATACGGAGAAATGTGGGGGCTGTATGAGGGGCAACAGCCC
NM_001278921.1      ------------------------------------------------------------
NM_057095.2         GACAGAGAATGTAATGAAAAATACGGAGAAATGTGGGGGCTGTATGAGGGGCAACAGCCC
                                                                                

HsCD00511709        ------------------------------------------------------------
NM_022820.4         ATGCTGGTCATCATGGATCCCGACATGATCAAAACAGTGTTAGTGAAAGAATGTTACTCT
NM_001278921.1      ------------------------------------------------------------
NM_057095.2         ATGCTGGTCATCATGGATCCCGACATGATCAAAACAGTGTTAGTGAAAGAATGTTACTCT
                                                                                

HsCD00511709        ------------------------------------------------------------
NM_022820.4         GTCTTCACAAACCAGATGCCTTTAGGTCCAATGGGATTTCTGAAAAGTGCCTTAAGTTTT
NM_001278921.1      ------------------------------------------------------------
NM_057095.2         GTCTTCACAAACCAGATGCCTTTAGGTCCAATGGGATTTCTGAAAAGTGCCTTAAGTTTT
                                                                                

HsCD00511709        ------------------------------------------------------------
NM_022820.4         GCTGAAGATGAAGAATGGAAGAGAATACGAACATTGCTATCTCCAGCTTTCACCAGTGTA
NM_001278921.1      ------------------------------------------------------------
NM_057095.2         GCTGAAGATGAAGAATGGAAGAGAATACGAACATTGCTATCTCCAGCTTTCACCAGTGTA
                                                                                

HsCD00511709        -------------------------------------------------ATTCCCAGCTG
NM_022820.4         AAATTCAAGGAAATGGTCCCCATCATTTCCCAATGTGGAGATATGTTGGTGAGAAGCCTG
NM_001278921.1      -------------------------------------------------ATTCCCAGCTG
NM_057095.2         AAATTCAAGGAAATGGTCCCCATCATTTCCCAATGTGGAGATATGTTGGTGAGAAGCCTG
                                                                     : : ... ***

HsCD00511709        GGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAAGCAGGAGGATCACC
NM_022820.4         AGGCAGGAAGCAGAGAACAGCAAGTCCATCAACTTGAAAGATTTCTTTGGGGCCTACACC
NM_001278921.1      GGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAAGCAGGAGGATCACC
NM_057095.2         AGGCAGGAAGCAGAGAACAGCAAGTCCATCAACTTGAAAGATTTCTTTGGGGCCTACACC
                    .*  :**:.**: * ..*:* **  *** **. ***..**. * .:  .**.  ::****

HsCD00511709        TAAGGTCAGGAGTTCGAGACCAGCTTTG--------------------------------
NM_022820.4         ATGGATGTAATCACTGGCACATTATTTGGAGTGAACTTGGATTCTCTCAACAATCCACAA
NM_001278921.1      TAAGGTCAGGAGTTCGAGACCAGCTTTG--------------------------------
NM_057095.2         ATGGATGTAATCACTGGCACATTATTTGGAGTGAACTTGGATTCTCTCAACAATCCACAA
                    ::.*.* :..: :  *. **.: .****                                

HsCD00511709        ---------CCAACATGGGTCTTTGGAATTTTGACAGAGAATGTAATGAAAAATACGGAG
NM_022820.4         GATCCCTTTCTGAAAAATATGAAGAAGCTTTTAAAATTGGATTTTTTGGATCCCTTTTTA
NM_001278921.1      ---------CCAACATGGGTCTTTGGAATTTTGACAGAGAATGTAATGAAAAATACGGAG
NM_057095.2         GATCCCTTTCTGAAAAATATGAAGAAGCTTTTAAAATTGGATTTTTTGGATCCCTTTTTA
                             * .*.*:. .* :: ....****.*.* :*.** *::**.*:.. :   :.

HsCD00511709        AAATGTGGGGCACTCTTTCCATTTCTTACCCCAGTTTTTGAAGCCCTAAATATCGGTTTG
NM_022820.4         CTCTTAATATCACTCTTTCCATTTCTTACCCCAGTTTTTGAAGCCCTAAATATCGGTTTG
NM_001278921.1      AAATGTGGGGCACTCTTTCCATTTCTTACCCCAGTTTTTGAAGCCCTAAATATCGGTTTG
NM_057095.2         CTCTTAATATCACTCTTTCCATTTCTTACCCCAGTTTTTGAAGCCCTAAATATCGGTTTG
                    .:.* :. . **************************************************

HsCD00511709        TTTCCAAAAGATGTTACCCATTTTTTAAAAAATTCCATTGAAAGGATGAAAGAAAGTCGC
NM_022820.4         TTTCCAAAAGATGTTACCCATTTTTTAAAAAATTCCATTGAAAGGATGAAAGAAAGTCGC
NM_001278921.1      TTTCCAAAAGATGTTACCCATTTTTTAAAAAATTCCATTGAAAGGATGAAAGAAAGTCGC
NM_057095.2         TTTCCAAAAGATGTTACCCATTTTTTAAAAAATTCCATTGAAAGGATGAAAGAAAGTCGC
                    ************************************************************

HsCD00511709        CTCAAAGATAAACAAAAGCATCGAGTAGATTTCTTTCAACAGATGATCGACTCCCAGAAT
NM_022820.4         CTCAAAGATAAACAAAAGCATCGAGTAGATTTCTTTCAACAGATGATCGACTCCCAGAAT
NM_001278921.1      CTCAAAGATAAACAAAAGCATCGAGTAGATTTCTTTCAACAGATGATCGACTCCCAGAAT
NM_057095.2         CTCAAAGATAAACAAAAGCATCGAGTAGATTTCTTTCAACAGATGATCGACTCCCAGAAT
                    ************************************************************

HsCD00511709        TCCAAAGAAACAAAGTCCCATAAAGCTCTGTCTGATCTGGAGCTTGTGGCCCAGTCAATT
NM_022820.4         TCCAAAGAAACAAAGTCCCATAAAGCTCTGTCTGATCTGGAGCTTGTGGCCCAGTCAATT
NM_001278921.1      TCCAAAGAAACAAAGTCCCATAAAGCTCTGTCTGATCTGGAGCTTGTGGCCCAGTCAATT
NM_057095.2         TCCAAAGAAACAAAGTCCCATAAAGCTCTGTCTGATCTGGAGCTTGTGGCCCAGTCAATT
                    ************************************************************

HsCD00511709        ATCATCATTTTTGCTGCCTATGACACAACTAGCACCACTCTCCCCTTCATTATGTATGAA
NM_022820.4         ATCATCATTTTTGCTGCCTATGACACAACTAGCACCACTCTCCCCTTCATTATGTATGAA
NM_001278921.1      ATCATCATTTTTGCTGCCTATGACACAACTAGCACCACTCTCCCCTTCATTATGTATGAA
NM_057095.2         ATCATCATTTTTGCTGCCTATGACACAACTAGCACCACTCTCCCCTTCATTATGTATGAA
                    ************************************************************

HsCD00511709        CTGGCCACTCACCCTGATGTCCAGCAGAAACTGCAGGAGGAGATTGACGCAGTTTTACCC
NM_022820.4         CTGGCCACTCACCCTGATGTCCAGCAGAAACTGCAGGAGGAGATTGACGCAGTTTTACCC
NM_001278921.1      CTGGCCACTCACCCTGATGTCCAGCAGAAACTGCAGGAGGAGATTGACGCAGTTTTACCC
NM_057095.2         CTGGCCACTCACCCTGATGTCCAGCAGAAACTGCAGGAGGAGATTGACGCAGTTTTACCC
                    ************************************************************

HsCD00511709        AATAAGGCACCTGTCACCTACGATGCCCTGGTACAGATGGAGTACCTTGACATGGTGGTG
NM_022820.4         AATAAGGCACCTGTCACCTACGATGCCCTGGTACAGATGGAGTACCTTGACATGGTGGTG
NM_001278921.1      AATAAGGCACCTGTCACCTACGATGCCCTGGTACAGATGGAGTACCTTGACATGGTGGTG
NM_057095.2         AATAAGGCACCTGTCACCTACGATGCCCTGGTACAGATGGAGTACCTTGACATGGTGGTG
                    ************************************************************

HsCD00511709        AATGAAACGCTCAGATTATTCCCAGTTGTTAGTAGAGTTACGAGAGTCTGCAAGAAAGAT
NM_022820.4         AATGAAACGCTCAGATTATTCCCAGTTGTTAGTAGAGTTACGAGAGTCTGCAAGAAAGAT
NM_001278921.1      AATGAAACGCTCAGATTATTCCCAGTTGTTAGTAGAGTTACGAGAGTCTGCAAGAAAGAT
NM_057095.2         AATGAAACGCTCAGATTATTCCCAGTTGTTAGTAGAGTTACGAGAGTCTGCAAGAAAGAT
                    ************************************************************

HsCD00511709        ATTGAAATCAATGGAGTGTTCATTCCCAAAGGGTTAGCAGTGATGGTTCCAATCTATGCT
NM_022820.4         ATTGAAATCAATGGAGTGTTCATTCCCAAAGGGTTAGCAGTGATGGTTCCAATCTATGCT
NM_001278921.1      ATTGAAATCAATGGAGTGTTCATTCCCAAAGGGTTAGCAGTGATGGTTCCAATCTATGCT
NM_057095.2         ATTGAAATCAATGGAGTGTTCATTCCCAAAGGGTTAGCAGTGATGGTTCCAATCTATGCT
                    ************************************************************

HsCD00511709        CTTCACCATGACCCAAAGTACTGGACAGAGCCTGAGAAGTTCTGCCCTGAAAG---GTTC
NM_022820.4         CTTCACCATGACCCAAAGTACTGGACAGAGCCTGAGAAGTTCTGCCCTGAAAGTAGGTTC
NM_001278921.1      CTTCACCATGACCCAAAGTACTGGACAGAGCCTGAGAAGTTCTGCCCTGAAAG---GTTC
NM_057095.2         CTTCACCATGACCCAAAGTACTGGACAGAGCCTGAGAAGTTCTGCCCTGAAAG---GTTC
                    *****************************************************   ****

HsCD00511709        AGTAAGAAGAACAAGGACAGCATAGATCTTTACAGATACATACCTTTTGGAGCTGGACCC
NM_022820.4         AGTAAGAAGAACAAGGACAGCATAGATCTTTACAGATACATACCTTTTGGAGCTGGACCC
NM_001278921.1      AGTAAGAAGAACAAGGACAGCATAGATCTTTACAGATACATACCTTTTGGAGCTGGACCC
NM_057095.2         AGTAAGAAGAACAAGGACAGCATAGATCTTTACAGATACATACCTTTTGGAGCTGGACCC
                    ************************************************************

HsCD00511709        CGAAACTGCATTGGCATGAGGTTTGCTCTCACAAACATAAAACTTGCTGTCATTAGAGCA
NM_022820.4         CGAAACTGCATTGGCATGAGGTTTGCTCTCACAAACATAAAACTTGCTGTCATTAGAGCA
NM_001278921.1      CGAAACTGCATTGGCATGAGGTTTGCTCTCACAAACATAAAACTTGCTGTCATTAGAGCA
NM_057095.2         CGAAACTGCATTGGCATGAGGTTTGCTCTCACAAACATAAAACTTGCTGTCATTAGAGCA
                    ************************************************************

HsCD00511709        CTGCAGAACTTCTCCTTCAAACCTTGTAAAGAGACTCAGATCCCACTGAAATTAGACAAT
NM_022820.4         CTGCAGAACTTCTCCTTCAAACCTTGTAAAGAGACTCAGATCCCACTGAAATTAGACAAT
NM_001278921.1      CTGCAGAACTTCTCCTTCAAACCTTGTAAAGAGACTCAGATCCCACTGAAATTAGACAAT
NM_057095.2         CTGCAGAACTTCTCCTTCAAACCTTGTAAAGAGACTCAGATCCCACTGAAATTAGACAAT
                    ************************************************************

HsCD00511709        CTACCAATTCTTCAACCAGAAAAACCTATTGTTCTAAAAGTGCACTTAAGAGATGGGATT
NM_022820.4         CTACCAATTCTTCAACCAGAAAAACCTATTGTTCTAAAAGTGCACTTAAGAGATGGGATT
NM_001278921.1      CTACCAATTCTTCAACCAGAAAAACCTATTGTTCTAAAAGTGCACTTAAGAGATGGGATT
NM_057095.2         CTACCAATTCTTCAACCAGAAAAACCTATTGTTCTAAAAGTGCACTTAAGAGATGGGATT
                    ************************************************************

HsCD00511709        ACAAGTGGACCC---
NM_022820.4         ACAAGTGGACCCTGA
NM_001278921.1      ACAAGTGGACCCTGA
NM_057095.2         ACAAGTGGACCCTGA
                    ************   

Pathways

Reactome : Biological oxidations
Reactome : Cytochrome P450 - arranged by substrate type
Reactome : Metabolism
Reactome : Miscellaneous substrates
Reactome : Phase 1 - Functionalization of compounds
Reactome : Xenobiotics
WikiPathway : cytochrome P450
WikiPathway : metapathway biotransformation

Protein

HPRD : 05942

Clone

Cloning Information : 668609
Original Clone ID : ccsbBroadEn_12497

Organism


TAX_ID : 9606
Species Specific ID: 64816

Genome

Chromosome : 7
Map Location : 7q21.1
Ensembl : ENSG00000021461

Reagents

Labome : CYP3A43-antibody