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FAM46B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FAM46B
Gene Name:  family with sequence similarity 46, member B
Sequence             
Original Clone ID: ccsbBroadEn_10215
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC012790.2,BC014160.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00511838

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1273nts         Open reading frame : 1 to 1273
Reference Sequence Alignment

ATGCCGTCGGAGAGCGGAGCTGAGCGCAGGGACCGGGCGGCTGCTCAGGTGGGGACGGCT
GCGGCCACGGCGGTGGCCACGGCAGCCCCGGCAGGCGGCGGCCCCGACCCGGAGGCCTTA
TCGGCCTTCCCCGGACGGCACCTGAGTGGGCTGAGCTGGCCACAGGTGAAGCGACTGGAC
GCTCTTCTGAGCGAGCCGATTCCCATTCACGGGCGCGGCAACTTCCCCACGCTGAGCGTG
CAGCCCCGGCAGATCGTGCAGGTGGTCCGCAGCACCCTGGAGGAGCAGGGACTACATGTG
CACAGTGTGCGGCTGCATGGTTCAGCTGCCAGCCACGTGCTGCACCCTGAGAGTGGCCTG
GGCTACAAGGATCTGGACCTGGTGTTCCGGGTGGACCTGCGCAGTGAGGCATCCTTCCAG
CTGACCAAGGCAGTGGTGCTGGCCTGCCTACTAGACTTCCTGCCGGCCGGTGTGAGCCGG
GCCAAGATCACGCCACTGACACTCAAGGAGGCATACGTGCAGAAGCTGGTGAAAGTGTGC
ACAGACTCGGACCGCTGGAGCCTCATCTCACTGTCCAACAAGAGCGGCAAGAACGTGGAG
CTCAAGTTTGTGGACTCGGTGAGACGCCAGTTTGAATTCAGCATAGACTCCTTCCAGATC
ATCCTGGACTCCCTGTTGCTCTTTGGCCAGTGCTCGTCCACTCCCATGTCTGAGGCCTTC
CACCCAACGGTCACAGGCGAAAGCCTGTACGGGGACTTCACCGAGGCCCTGGAGCACCTG
CGGCACCGTGTCATCGCCACGCGCAGTCCCGAGGAGATCCGAGGTGGTGGCCTCCTCAAG
TACTGCCACCTCCTGGTGCGGGGCTTCCGGCCCCGGCCCAGCACCGATGTGCGCGCCCTG
CAGCGCTACATGTGCTCCCGCTTCTTCATCGACTTTCCAGACCTGGTGGAGCAGCGGCGC
ACCCTAGAGCGCTACCTGGAGGCCCACTTCGGTGGGGCAGATGCAGCCCGCCGTTACGCC
TGCCTGGTGACACTGCACCGGGTGGTCAACGAGAGCACCGTGTGCCTCATGAACCACGAG
CGCCGCCAGACGCTGGACCTCATTGCCGCACTGGCGCTGCAGGCACTGGCTGAGCAGGGC
CCAGCTGCCACTGCCGCCCTGGCCTGGCGCCCTCCAGGCACTGACGGGGTTGTGCCAGCC
ACTGTCAATTACTACGTGACCCCCGTGCAACCTCTCCTGGCTCACGCCTATCCCACCTGG
CTGCCTTGTAAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FAM46B
Symbol Nomenclature : FAM46B
SYNONYM : RP11-344H11.8
Designation : protein FAM46B
Full Nomenclature : family with sequence similarity 46, member B
GENEID : 115572
GenBank Accession : EU176773
HGNC : 28273
Vega : OTTHUMG00000004278
Target GenBank: EU176773

 

Reference Sequence Alignment

  

HsCD00511838      ---ATGCCGTCGGAGAGCGGAGCTGAGCGCAGGGACCGGGCGGCTGCTCAGGTGGGGACG
NM_052943.3       ATGATGCCGTCGGAGAGCGGAGCTGAGCGCAGGGACCGGGCGGCTGCTCAGGTGGGGACG
                     *********************************************************

HsCD00511838      GCTGCGGCCACGGCGGTGGCCACGGCAGCCCCGGCAGGCGGCGGCCCCGACCCGGAGGCC
NM_052943.3       GCTGCGGCCACGGCGGTGGCCACGGCAGCCCCGGCAGGCGGCGGCCCCGACCCGGAGGCC
                  ************************************************************

HsCD00511838      TTATCGGCCTTCCCCGGACGGCACCTGAGTGGGCTGAGCTGGCCACAGGTGAAGCGACTG
NM_052943.3       TTATCGGCCTTCCCCGGACGGCACCTGAGTGGGCTGAGCTGGCCACAGGTGAAGCGACTG
                  ************************************************************

HsCD00511838      GACGCTCTTCTGAGCGAGCCGATTCCCATTCACGGGCGCGGCAACTTCCCCACGCTGAGC
NM_052943.3       GACGCTCTTCTGAGCGAGCCGATTCCCATTCACGGGCGCGGCAACTTCCCCACGCTGAGC
                  ************************************************************

HsCD00511838      GTGCAGCCCCGGCAGATCGTGCAGGTGGTCCGCAGCACCCTGGAGGAGCAGGGACTACAT
NM_052943.3       GTGCAGCCCCGGCAGATCGTGCAGGTGGTCCGCAGCACCCTGGAGGAGCAGGGACTACAT
                  ************************************************************

HsCD00511838      GTGCACAGTGTGCGGCTGCATGGTTCAGCTGCCAGCCACGTGCTGCACCCTGAGAGTGGC
NM_052943.3       GTGCACAGTGTGCGGCTGCATGGTTCAGCTGCCAGCCACGTGCTGCACCCTGAGAGTGGC
                  ************************************************************

HsCD00511838      CTGGGCTACAAGGATCTGGACCTGGTGTTCCGGGTGGACCTGCGCAGTGAGGCATCCTTC
NM_052943.3       CTGGGCTACAAGGATCTGGACCTGGTGTTCCGGGTGGACCTGCGCAGTGAGGCATCCTTC
                  ************************************************************

HsCD00511838      CAGCTGACCAAGGCAGTGGTGCTGGCCTGCCTACTAGACTTCCTGCCGGCCGGTGTGAGC
NM_052943.3       CAGCTGACCAAGGCAGTGGTGCTGGCCTGCCTACTAGACTTCCTGCCGGCCGGTGTGAGC
                  ************************************************************

HsCD00511838      CGGGCCAAGATCACGCCACTGACACTCAAGGAGGCATACGTGCAGAAGCTGGTGAAAGTG
NM_052943.3       CGGGCCAAGATCACGCCACTGACACTCAAGGAGGCATACGTGCAGAAGCTGGTGAAAGTG
                  ************************************************************

HsCD00511838      TGCACAGACTCGGACCGCTGGAGCCTCATCTCACTGTCCAACAAGAGCGGCAAGAACGTG
NM_052943.3       TGCACAGACTCGGACCGCTGGAGCCTCATCTCACTGTCCAACAAGAGCGGCAAGAACGTG
                  ************************************************************

HsCD00511838      GAGCTCAAGTTTGTGGACTCGGTGAGACGCCAGTTTGAATTCAGCATAGACTCCTTCCAG
NM_052943.3       GAGCTCAAGTTTGTGGACTCGGTGAGACGCCAGTTTGAATTCAGCATAGACTCCTTCCAG
                  ************************************************************

HsCD00511838      ATCATCCTGGACTCCCTGTTGCTCTTTGGCCAGTGCTCGTCCACTCCCATGTCTGAGGCC
NM_052943.3       ATCATCCTGGACTCCCTGTTGCTCTTTGGCCAGTGCTCGTCCACTCCCATGTCTGAGGCC
                  ************************************************************

HsCD00511838      TTCCACCCAACGGTCACAGGCGAAAGCCTGTACGGGGACTTCACCGAGGCCCTGGAGCAC
NM_052943.3       TTCCACCCAACGGTCACAGGCGAAAGCCTGTACGGGGACTTCACCGAGGCCCTGGAGCAC
                  ************************************************************

HsCD00511838      CTGCGGCACCGTGTCATCGCCACGCGCAGTCCCGAGGAGATCCGAGGTGGTGGCCTCCTC
NM_052943.3       CTGCGGCACCGTGTCATCGCCACGCGCAGTCCCGAGGAGATCCGAGGTGGTGGCCTCCTC
                  ************************************************************

HsCD00511838      AAGTACTGCCACCTCCTGGTGCGGGGCTTCCGGCCCCGGCCCAGCACCGATGTGCGCGCC
NM_052943.3       AAGTACTGCCACCTCCTGGTGCGGGGCTTCCGGCCCCGGCCCAGCACCGATGTGCGCGCC
                  ************************************************************

HsCD00511838      CTGCAGCGCTACATGTGCTCCCGCTTCTTCATCGACTTTCCAGACCTGGTGGAGCAGCGG
NM_052943.3       CTGCAGCGCTACATGTGCTCCCGCTTCTTCATCGACTTTCCAGACCTGGTGGAGCAGCGG
                  ************************************************************

HsCD00511838      CGCACCCTAGAGCGCTACCTGGAGGCCCACTTCGGTGGGGCAGATGCAGCCCGCCGTTAC
NM_052943.3       CGCACCCTAGAGCGCTACCTGGAGGCCCACTTCGGTGGGGCAGATGCAGCCCGCCGTTAC
                  ************************************************************

HsCD00511838      GCCTGCCTGGTGACACTGCACCGGGTGGTCAACGAGAGCACCGTGTGCCTCATGAACCAC
NM_052943.3       GCCTGCCTGGTGACACTGCACCGGGTGGTCAACGAGAGCACCGTGTGCCTCATGAACCAC
                  ************************************************************

HsCD00511838      GAGCGCCGCCAGACGCTGGACCTCATTGCCGCACTGGCGCTGCAGGCACTGGCTGAGCAG
NM_052943.3       GAGCGCCGCCAGACGCTGGACCTCATTGCCGCACTGGCGCTGCAGGCACTGGCTGAGCAG
                  ************************************************************

HsCD00511838      GGCCCAGCTGCCACTGCCGCCCTGGCCTGGCGCCCTCCAGGCACTGACGGGGTTGTGCCA
NM_052943.3       GGCCCAGCTGCCACTGCCGCCCTGGCCTGGCGCCCTCCAGGCACTGACGGGGTTGTGCCA
                  ************************************************************

HsCD00511838      GCCACTGTCAATTACTACGTGACCCCCGTGCAACCTCTCCTGGCTCACGCCTATCCCACC
NM_052943.3       GCCACTGTCAATTACTACGTGACCCCCGTGCAACCTCTCCTGGCTCACGCCTATCCCACC
                  ************************************************************

HsCD00511838      TGGCTGCCTTGTAAC---
NM_052943.3       TGGCTGCCTTGTAACTGA
                  ***************   

Protein

SMART domain : DUF1693 : Domain of unknown function (DUF1693)
HPRD : 16882

Clone

Cloning Information : 605143
Original Clone ID : ccsbBroadEn_10215

Organism


TAX_ID : 9606
Species Specific ID: 115572

Genome

Chromosome : 1
Map Location : 1p36.11
Ensembl : ENSG00000158246

Reagents

Labome : FAM46B-siRNA