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THOC1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  THOC1
Gene Name:  THO complex 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH141370.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00005119

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1974nts         Open reading frame : 1 to 1974
Reference Sequence Alignment

ATGTCTCCGACGCCGCCGCTCTTCAGTTTGCCCGAAGCGCGGACGCGGTTTACGAAGTCT
ACCAGAGAGGCCTTGAACAACAAAAACATCAAGCCATTGTTAAGTACCTTCAGCCAGGTA
CCTGGCAGTGAAAATGAAAAAAAATGTACCCTTGACCAAGCTTTCAGAGGTATTCTAGAA
GAAGAAATTATAAATCATTCATCATGTGAAAACGTTTTAGCTATTATTTCTCTTGCTATT
GGGGGAGTAACTGAAGGTATTTGTACCGCATCTACACCTTTTGTATTGTTGGGAGATGTT
TTGGATTGTCTTCCTTTGGATCAGTGTGACACAATATTCACTTTTGTGGAAAAAAATGTT
GCTACTTGGAAATCAAATACATTCTATTCTGCTGGGAAAAATTACTTACTACGTATGTGC
AATGATCTCCTAAGAAGATTGTCTAAATCCCAGAATACAGTCTTCTGTGGACGGATTCAG
CTCTTTTTGGCCAGGCTTTTCCCTCTGTCTGAGAAATCAGGTCTTAACTTGCAGAGTCAG
TTTAATCTGGAAAATGTCACTGTTTTCAATACAAATGAGCAGGAAAGCACCCTGGGTCAG
AAGCACACTGAAGATAGAGAAGAAGGAATGGATGTAGAAGAAGGCGAAATGGGAGATGAG
GAAGCTCCAACAACGTGCTCTATTCCAATTGATTACAACCTGTATCGAAAATTCTGGTCA
CTTCAGGATTACTTCAGGAACCCTGTGCAATGCTATGAGAAGATTTCATGGAAAACTTTT
CTCAAGTATTCTGAAGAAGTTTTAGCTGTTTTTAAGAGTTATAAATTAGATGATACTCAG
GCCTCAAGAAAAAAGATGGAAGAATTGAAAACAGGAGGAGAACATGTATATTTTGCAAAA
TTTTTAACAAGTGAAAAGCTGATGGATTTACAACTGAGTGACAGTAACTTTCGTCGACAC
ATCCTGTTGCAGTATCTCATTTTATTCCAATATCTCAAGGGGCAGGTCAAATTCAAAAGT
TCAAACTATGTTTTAACTGATGAGCAATCACTTTGGATTGAAGATACTACAAAATCAGTT
TATCAACTACTATCTGAAAACCCCCCCGATGGAGAAAGATTTTCAAAGATGGTAGAGCAT
ATATTAAACACTGAAGAAAACTGGAACTCGTGGAAAAATGAAGGTTGCCCAAGTTTTGTG
AAAGAAAGAACATCAGATACCAAACCTACGAGAATAATTCGGAAGAGAACAGCACCCGAG
GACTTCCTAGGGAAAGGACCCACCAAAAAAATTCTGATGGGAAATGAGGAGTTAACAAGG
CTTTGGAATCTTTGCCCTGATAATATGGAAGCCTGTAAATCAGAGACAAGGGAACACATG
CCCACTTTGGAGGAATTCTTTGAAGAAGCCATTGAACAGGCAGACCCTGAAAATATGGTG
GAAAATGAATATAAGGCTGTGAACAATTCAAATTATGGTTGGAGAGCCCTGAGACTATTA
GCACGGAGAAGCCCTCACTTCTTCCAGCCAACCAACCAGCAGTTTAAAAGTTTACCAGAA
TATCTTGAAAATATGGTAATAAAGCTAGCCAAGGAATTACCGCCTCCTTCTGAAGAAATA
AAAACAGGTGAGGATGAAGATGAGGAAGATAATGATGCTCTACTGAAGGAAAATGAAAGT
CCTGATGTTCGGCGAGACAAACCTGTAACAGGAGAACAAATAGAGGTATTTGCCAACAAG
CTGGGTGAACAATGGAAGATTCTGGCTCCCTACTTGGAAATGAAAGACTCAGAAATTAGG
CAGATTGAGTGTGACAGTGAAGACATGAAGATGAGAGCTAAGCAGCTCCTGGTTGCCTGG
CAAGATCAAGAGGGAGTTCATGCAACACCTGAGAATCTGATTAATGCACTGAATAAGTCT
GGATTAAGTGACCTTGCAGAAAGTCTAACTAATGACAATGAGACAAATAGTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2005
Start on reference sequence 32
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : THOC1
Symbol Nomenclature : THOC1
SYNONYM : HPR1; P84; P84N5
Designation : THO complex subunit 1|hTREX84|nuclear matrix protein p84|tho1
Full Nomenclature : THO complex 1
GENEID : 9984
GI : 60654240
GenBank Accession : AY892896
HGNC : 19070
MIM : 606930
Vega : OTTHUMG00000178051
Target GenBank: BC010381

 

Reference Sequence Alignment

  

HsCD00005119      ATGTCTCCGACGCCGCCGCTCTTCAGTTTGCCCGAAGCGCGGACGCGGTTTACGAAGTCT
NM_005131.2       ATGTCTCCGACGCCGCCGCTCTTCAGTTTGCCCGAAGCGCGGACGCGGTTTACGAAGTCT
                  ************************************************************

HsCD00005119      ACCAGAGAGGCCTTGAACAACAAAAACATCAAGCCATTGTTAAGTACCTTCAGCCAGGTA
NM_005131.2       ACCAGAGAGGCCTTGAACAACAAAAACATCAAGCCATTGTTAAGTACCTTCAGCCAGGTA
                  ************************************************************

HsCD00005119      CCTGGCAGTGAAAATGAAAAAAAATGTACCCTTGACCAAGCTTTCAGAGGTATTCTAGAA
NM_005131.2       CCTGGCAGTGAAAATGAAAAAAAATGTACCCTTGACCAAGCTTTCAGAGGTATTCTAGAA
                  ************************************************************

HsCD00005119      GAAGAAATTATAAATCATTCATCATGTGAAAACGTTTTAGCTATTATTTCTCTTGCTATT
NM_005131.2       GAAGAAATTATAAATCATTCATCATGTGAAAACGTTTTAGCTATTATTTCTCTTGCTATT
                  ************************************************************

HsCD00005119      GGGGGAGTAACTGAAGGTATTTGTACCGCATCTACACCTTTTGTATTGTTGGGAGATGTT
NM_005131.2       GGGGGAGTAACTGAAGGTATTTGTACCGCATCTACACCTTTTGTATTGTTGGGAGATGTT
                  ************************************************************

HsCD00005119      TTGGATTGTCTTCCTTTGGATCAGTGTGACACAATATTCACTTTTGTGGAAAAAAATGTT
NM_005131.2       TTGGATTGTCTTCCTTTGGATCAGTGTGACACAATATTCACTTTTGTGGAAAAAAATGTT
                  ************************************************************

HsCD00005119      GCTACTTGGAAATCAAATACATTCTATTCTGCTGGGAAAAATTACTTACTACGTATGTGC
NM_005131.2       GCTACTTGGAAATCAAATACATTCTATTCTGCTGGGAAAAATTACTTACTACGTATGTGC
                  ************************************************************

HsCD00005119      AATGATCTCCTAAGAAGATTGTCTAAATCCCAGAATACAGTCTTCTGTGGACGGATTCAG
NM_005131.2       AATGATCTCCTAAGAAGATTGTCTAAATCCCAGAATACAGTCTTCTGTGGACGGATTCAG
                  ************************************************************

HsCD00005119      CTCTTTTTGGCCAGGCTTTTCCCTCTGTCTGAGAAATCAGGTCTTAACTTGCAGAGTCAG
NM_005131.2       CTCTTTTTGGCCAGGCTTTTCCCTCTGTCTGAGAAATCAGGTCTTAACTTGCAGAGTCAG
                  ************************************************************

HsCD00005119      TTTAATCTGGAAAATGTCACTGTTTTCAATACAAATGAGCAGGAAAGCACCCTGGGTCAG
NM_005131.2       TTTAATCTGGAAAATGTCACTGTTTTCAATACAAATGAGCAGGAAAGCACCCTGGGTCAG
                  ************************************************************

HsCD00005119      AAGCACACTGAAGATAGAGAAGAAGGAATGGATGTAGAAGAAGGCGAAATGGGAGATGAG
NM_005131.2       AAGCACACTGAAGATAGAGAAGAAGGAATGGATGTAGAAGAAGGCGAAATGGGAGACGAG
                  ******************************************************** ***

HsCD00005119      GAAGCTCCAACAACGTGCTCTATTCCAATTGATTACAACCTGTATCGAAAATTCTGGTCA
NM_005131.2       GAAGCTCCAACAACGTGCTCTATTCCAATTGATTACAACCTGTATCGAAAATTCTGGTCA
                  ************************************************************

HsCD00005119      CTTCAGGATTACTTCAGGAACCCTGTGCAATGCTATGAGAAGATTTCATGGAAAACTTTT
NM_005131.2       CTTCAGGATTACTTCAGGAACCCTGTGCAATGCTATGAGAAGATTTCATGGAAAACTTTT
                  ************************************************************

HsCD00005119      CTCAAGTATTCTGAAGAAGTTTTAGCTGTTTTTAAGAGTTATAAATTAGATGATACTCAG
NM_005131.2       CTCAAGTATTCTGAAGAAGTTTTAGCTGTTTTTAAGAGTTATAAATTAGATGATACTCAG
                  ************************************************************

HsCD00005119      GCCTCAAGAAAAAAGATGGAAGAATTGAAAACAGGAGGAGAACATGTATATTTTGCAAAA
NM_005131.2       GCCTCAAGAAAAAAGATGGAAGAATTGAAAACAGGAGGAGAACATGTATATTTTGCAAAA
                  ************************************************************

HsCD00005119      TTTTTAACAAGTGAAAAGCTGATGGATTTACAACTGAGTGACAGTAACTTTCGTCGACAC
NM_005131.2       TTTTTAACAAGTGAAAAGCTGATGGATTTACAACTGAGTGACAGTAACTTTCGTCGACAC
                  ************************************************************

HsCD00005119      ATCCTGTTGCAGTATCTCATTTTATTCCAATATCTCAAGGGGCAGGTCAAATTCAAAAGT
NM_005131.2       ATCCTGTTGCAGTATCTCATTTTATTCCAATATCTCAAGGGGCAGGTCAAATTCAAAAGT
                  ************************************************************

HsCD00005119      TCAAACTATGTTTTAACTGATGAGCAATCACTTTGGATTGAAGATACTACAAAATCAGTT
NM_005131.2       TCAAACTATGTTTTAACTGATGAGCAATCACTTTGGATTGAAGATACTACAAAATCAGTT
                  ************************************************************

HsCD00005119      TATCAACTACTATCTGAAAACCCCCCCGATGGAGAAAGATTTTCAAAGATGGTAGAGCAT
NM_005131.2       TATCAACTACTATCTGAAAACCCCCCCGATGGAGAAAGATTTTCAAAGATGGTAGAGCAT
                  ************************************************************

HsCD00005119      ATATTAAACACTGAAGAAAACTGGAACTCGTGGAAAAATGAAGGTTGCCCAAGTTTTGTG
NM_005131.2       ATATTAAACACTGAAGAAAACTGGAACTCGTGGAAAAATGAAGGTTGCCCAAGTTTTGTG
                  ************************************************************

HsCD00005119      AAAGAAAGAACATCAGATACCAAACCTACGAGAATAATTCGGAAGAGAACAGCACCCGAG
NM_005131.2       AAAGAAAGAACATCAGATACCAAACCTACGAGAATAATTCGGAAGAGAACAGCACCCGAG
                  ************************************************************

HsCD00005119      GACTTCCTAGGGAAAGGACCCACCAAAAAAATTCTGATGGGAAATGAGGAGTTAACAAGG
NM_005131.2       GACTTCCTAGGGAAAGGACCCACCAAAAAAATTCTGATGGGAAATGAGGAGTTAACAAGG
                  ************************************************************

HsCD00005119      CTTTGGAATCTTTGCCCTGATAATATGGAAGCCTGTAAATCAGAGACAAGGGAACACATG
NM_005131.2       CTTTGGAATCTTTGCCCTGATAATATGGAAGCCTGTAAATCAGAGACAAGGGAACACATG
                  ************************************************************

HsCD00005119      CCCACTTTGGAGGAATTCTTTGAAGAAGCCATTGAACAGGCAGACCCTGAAAATATGGTG
NM_005131.2       CCCACTTTGGAGGAATTCTTTGAAGAAGCCATTGAACAGGCAGACCCTGAAAATATGGTG
                  ************************************************************

HsCD00005119      GAAAATGAATATAAGGCTGTGAACAATTCAAATTATGGTTGGAGAGCCCTGAGACTATTA
NM_005131.2       GAAAATGAATATAAGGCTGTGAACAATTCAAATTATGGTTGGAGAGCCCTGAGACTATTA
                  ************************************************************

HsCD00005119      GCACGGAGAAGCCCTCACTTCTTCCAGCCAACCAACCAGCAGTTTAAAAGTTTACCAGAA
NM_005131.2       GCACGGAGAAGCCCTCACTTCTTCCAGCCAACCAACCAGCAGTTTAAAAGTTTACCAGAA
                  ************************************************************

HsCD00005119      TATCTTGAAAATATGGTAATAAAGCTAGCCAAGGAATTACCGCCTCCTTCTGAAGAAATA
NM_005131.2       TATCTTGAAAATATGGTAATAAAGCTAGCCAAGGAATTACCGCCTCCTTCTGAAGAAATA
                  ************************************************************

HsCD00005119      AAAACAGGTGAGGATGAAGATGAGGAAGATAATGATGCTCTACTGAAGGAAAATGAAAGT
NM_005131.2       AAAACAGGTGAGGATGAAGATGAGGAAGATAATGATGCTCTACTGAAGGAAAATGAAAGT
                  ************************************************************

HsCD00005119      CCTGATGTTCGGCGAGACAAACCTGTAACAGGAGAACAAATAGAGGTATTTGCCAACAAG
NM_005131.2       CCTGATGTTCGGCGAGACAAACCTGTAACAGGAGAACAAATAGAGGTATTTGCCAACAAG
                  ************************************************************

HsCD00005119      CTGGGTGAACAATGGAAGATTCTGGCTCCCTACTTGGAAATGAAAGACTCAGAAATTAGG
NM_005131.2       CTGGGTGAACAATGGAAGATTCTGGCTCCCTACTTGGAAATGAAAGACTCAGAAATTAGG
                  ************************************************************

HsCD00005119      CAGATTGAGTGTGACAGTGAAGACATGAAGATGAGAGCTAAGCAGCTCCTGGTTGCCTGG
NM_005131.2       CAGATTGAGTGTGACAGTGAAGACATGAAGATGAGAGCTAAGCAGCTCCTGGTTGCCTGG
                  ************************************************************

HsCD00005119      CAAGATCAAGAGGGAGTTCATGCAACACCTGAGAATCTGATTAATGCACTGAATAAGTCT
NM_005131.2       CAAGATCAAGAGGGAGTTCATGCAACACCTGAGAATCTGATTAATGCACTGAATAAGTCT
                  ************************************************************

HsCD00005119      GGATTAAGTGACCTTGCAGAAAGTCTAACTAATGACAATGAGACAAATAGTTTG
NM_005131.2       GGATTAAGTGACCTTGCAGAAAGTCTAACTAATGACAATGAGACAAATAGTTAG
                  ****************************************************:*

Clone

Cloning Information : 141370
HIP Master Clone ID : 117637
Original Clone ID : FLH141370.01L

Organism


TAX_ID : 9606
Species Specific ID: 9984

Genome

Chromosome : 18
Map Location : 18p11.32
Ensembl : ENSG00000079134

Reagents

Labome : THOC1-antibody