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ITGB8 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ITGB8
Gene Name:  integrin, beta 8
Sequence             
Original Clone ID: ccsbBroadEn_10927
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC002630.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00511912

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1318nts        
Open reading frame : 1 to 1318
Reference Sequence Alignment

ATGTGCGGCTCGGCCCTGGCTTTTTTTACCGCTGCATTTGTCTGCCTGCAAAACGACCGG
CGAGGTCCCGCCTCGTTCCTCTGGGCAGCCTGGGTGTTTTCACTTGTTCTTGGACTGGGC
CAAGGTGAAGACAATAGATGTGCATCTTCAAATGCAGCATCCTGTGCCAGGTGCCTTGCG
CTGGGTCCAGAATGTGGATGGTGTGTTCAAGAGGATTTCATTTCAGGTGGATCAAGAAGT
GAACGTTGTGATATTGTTTCCAATTTAATAAGCAAAGGCTGCTCAGTTGATTCAATAGAA
TACCCATCTGTGCATGTTATAATACCCACTGAAAATGAAATTAATACCCAGGTGACACCA
GGAGAAGTGTCTATCCAGCTGCGTCCAGGAGCCGAAGCTAATTTTATGCTGAAAGTTCAT
CCTCTGAAGAAATATCCTGTGGATCTTTATTATCTTGTTGATGTCTCAGCATCAATGCAC
AATAATATAGAAAAATTAAATTCCGTTGGAAACGATTTATCTAGAAAAATGGCATTTTTC
TCCCGTGACTTTCGTCTTGGATTTGGCTCATACGTTGATAAAACAGTTTCACCATACATT
AGCATCCACCCCGAAAGGATTCATAATCAATGCAGTGACTACAATTTAGACTGCATGCCT
CCCCATGGATACATCCATGTGCTGTCTTTGACAGAGAACATCACTGAGTTTGAGAAAGCA
GTTCATAGACAGAAGATCTCTGGAAACATAGATACACCAGAAGGAGGTTTTGACGCCATG
CTTCAGGCAGCTGTCTGTGAAAGTCATATCGGATGGCGAAAAGAGGCTAAAAGATTGCTG
CTGGTGATGACAGATCAGACGTCTCATCTCGCTCTTGATAGCAAATTGGCAGGCATAGTG
GTGCCCAATGACGGAAACTGTCATCTGAAAAACAACGTCTATGTCAAATCGACAACCATG
GAACACCCCTCACTAGGCCAACTTTCAGAGAAATTAATAGACAACAACATTAATGTCATC
TTTGCAGTTCAAGGAAAACAATTTCATTGGTATAAGGATCTTCTACCCCTCTTGCCAGGC
ACCATTGCTGGTGAAATAGAATCAAAGGCTGCAAACCTCAATAATTTGGTAGTGGAAGCC
TATCAGAAGCTCATTTCAGAAGTGAAAGTTCAGGTGGAAAACCAGGTACAAGGCATCTAT
TTTAACATTACCGCCATCTGTCCAGATGGGTCCAGAAAGCCAGGCATGGAAGGATGCAGA
AACGTGACGAGCAATGATGAAGTATGTGGGTGTGCATTTTTCCCTTTTAAAATAAAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ITGB8
Symbol Nomenclature : ITGB8
Designation : integrin beta-8
Full Nomenclature : integrin, beta 8
GENEID : 3696
HGNC : 6163
MIM : 604160
Vega : OTTHUMG00000023594
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00511912      ATGTGCGGCTCGGCCCTGGCTTTTTTTACCGCTGCATTTGTCTGCCTGCAAAACGACCGG
NM_002214.2       ATGTGCGGCTCGGCCCTGGCTTTTTTTACCGCTGCATTTGTCTGCCTGCAAAACGACCGG
                  ************************************************************

HsCD00511912      CGAGGTCCCGCCTCGTTCCTCTGGGCAGCCTGGGTGTTTTCACTTGTTCTTGGACTGGGC
NM_002214.2       CGAGGTCCCGCCTCGTTCCTCTGGGCAGCCTGGGTGTTTTCACTTGTTCTTGGACTGGGC
                  ************************************************************

HsCD00511912      CAAGGTGAAGACAATAGATGTGCATCTTCAAATGCAGCATCCTGTGCCAGGTGCCTTGCG
NM_002214.2       CAAGGTGAAGACAATAGATGTGCATCTTCAAATGCAGCATCCTGTGCCAGGTGCCTTGCG
                  ************************************************************

HsCD00511912      CTGGGTCCAGAATGTGGATGGTGTGTTCAAGAGGATTTCATTTCAGGTGGATCAAGAAGT
NM_002214.2       CTGGGTCCAGAATGTGGATGGTGTGTTCAAGAGGATTTCATTTCAGGTGGATCAAGAAGT
                  ************************************************************

HsCD00511912      GAACGTTGTGATATTGTTTCCAATTTAATAAGCAAAGGCTGCTCAGTTGATTCAATAGAA
NM_002214.2       GAACGTTGTGATATTGTTTCCAATTTAATAAGCAAAGGCTGCTCAGTTGATTCAATAGAA
                  ************************************************************

HsCD00511912      TACCCATCTGTGCATGTTATAATACCCACTGAAAATGAAATTAATACCCAGGTGACACCA
NM_002214.2       TACCCATCTGTGCATGTTATAATACCCACTGAAAATGAAATTAATACCCAGGTGACACCA
                  ************************************************************

HsCD00511912      GGAGAAGTGTCTATCCAGCTGCGTCCAGGAGCCGAAGCTAATTTTATGCTGAAAGTTCAT
NM_002214.2       GGAGAAGTGTCTATCCAGCTGCGTCCAGGAGCCGAAGCTAATTTTATGCTGAAAGTTCAT
                  ************************************************************

HsCD00511912      CCTCTGAAGAAATATCCTGTGGATCTTTATTATCTTGTTGATGTCTCAGCATCAATGCAC
NM_002214.2       CCTCTGAAGAAATATCCTGTGGATCTTTATTATCTTGTTGATGTCTCAGCATCAATGCAC
                  ************************************************************

HsCD00511912      AATAATATAGAAAAATTAAATTCCGTTGGAAACGATTTATCTAGAAAAATGGCATTTTTC
NM_002214.2       AATAATATAGAAAAATTAAATTCCGTTGGAAACGATTTATCTAGAAAAATGGCATTTTTC
                  ************************************************************

HsCD00511912      TCCCGTGACTTTCGTCTTGGATTTGGCTCATACGTTGATAAAACAGTTTCACCATACATT
NM_002214.2       TCCCGTGACTTTCGTCTTGGATTTGGCTCATACGTTGATAAAACAGTTTCACCATACATT
                  ************************************************************

HsCD00511912      AGCATCCACCCCGAAAGGATTCATAATCAATGCAGTGACTACAATTTAGACTGCATGCCT
NM_002214.2       AGCATCCACCCCGAAAGGATTCATAATCAATGCAGTGACTACAATTTAGACTGCATGCCT
                  ************************************************************

HsCD00511912      CCCCATGGATACATCCATGTGCTGTCTTTGACAGAGAACATCACTGAGTTTGAGAAAGCA
NM_002214.2       CCCCATGGATACATCCATGTGCTGTCTTTGACAGAGAACATCACTGAGTTTGAGAAAGCA
                  ************************************************************

HsCD00511912      GTTCATAGACAGAAGATCTCTGGAAACATAGATACACCAGAAGGAGGTTTTGACGCCATG
NM_002214.2       GTTCATAGACAGAAGATCTCTGGAAACATAGATACACCAGAAGGAGGTTTTGACGCCATG
                  ************************************************************

HsCD00511912      CTTCAGGCAGCTGTCTGTGAAAGTCATATCGGATGGCGAAAAGAGGCTAAAAGATTGCTG
NM_002214.2       CTTCAGGCAGCTGTCTGTGAAAGTCATATCGGATGGCGAAAAGAGGCTAAAAGATTGCTG
                  ************************************************************

HsCD00511912      CTGGTGATGACAGATCAGACGTCTCATCTCGCTCTTGATAGCAAATTGGCAGGCATAGTG
NM_002214.2       CTGGTGATGACAGATCAGACGTCTCATCTCGCTCTTGATAGCAAATTGGCAGGCATAGTG
                  ************************************************************

HsCD00511912      GTGCCCAATGACGGAAACTGTCATCTGAAAAACAACGTCTATGTCAAATCGACAACCATG
NM_002214.2       GTGCCCAATGACGGAAACTGTCATCTGAAAAACAACGTCTATGTCAAATCGACAACCATG
                  ************************************************************

HsCD00511912      GAACACCCCTCACTAGGCCAACTTTCAGAGAAATTAATAGACAACAACATTAATGTCATC
NM_002214.2       GAACACCCCTCACTAGGCCAACTTTCAGAGAAATTAATAGACAACAACATTAATGTCATC
                  ************************************************************

HsCD00511912      TTTGCAGTTCAAGGAAAACAATTTCATTGGTATAAGGATCTTCTACCCCTCTTGCCAGGC
NM_002214.2       TTTGCAGTTCAAGGAAAACAATTTCATTGGTATAAGGATCTTCTACCCCTCTTGCCAGGC
                  ************************************************************

HsCD00511912      ACCATTGCTGGTGAAATAGAATCAAAGGCTGCAAACCTCAATAATTTGGTAGTGGAAGCC
NM_002214.2       ACCATTGCTGGTGAAATAGAATCAAAGGCTGCAAACCTCAATAATTTGGTAGTGGAAGCC
                  ************************************************************

HsCD00511912      TATCAGAAGCTCATTTCAGAAGTGAAAGTTCAGGTGGAAAACCAGGTACAAGGCATCTAT
NM_002214.2       TATCAGAAGCTCATTTCAGAAGTGAAAGTTCAGGTGGAAAACCAGGTACAAGGCATCTAT
                  ************************************************************

HsCD00511912      TTTAACATTACCGCCATCTGTCCAGATGGGTCCAGAAAGCCAGGCATGGAAGGATGCAGA
NM_002214.2       TTTAACATTACCGCCATCTGTCCAGATGGGTCCAGAAAGCCAGGCATGGAAGGATGCAGA
                  ************************************************************

HsCD00511912      AACGTGACGAGCAATGATGAAGTATGTGGGTGTGCATTTTTCCCTTTTAAAATAAAC---
NM_002214.2       AACGTGACGAGCAATGATGAAGTTCTTTTCAATGTAACAGTTACAATGAAAAAATGTGAT
                  ***********************:  *   :.** *: : * .*::* ****:*:.    

HsCD00511912      ------------------------------------------------------------
NM_002214.2       GTCACAGGAGGAAAAAACTATGCAATAATCAAACCTATTGGTTTTAATGAAACCGCTAAA
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       ATTCATATACACAGAAACTGCAGCTGTCAGTGTGAGGACAACAGAGGACCTAAAGGAAAG
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       TGTGTAGATGAAACTTTTCTAGATTCCAAGTGTTTCCAGTGTGATGAGAATAAATGTCAT
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       TTTGATGAAGATCAGTTTTCTTCTGAGAGTTGCAAGTCACACAAGGATCAGCCTGTTTGC
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       AGTGGTCGAGGAGTTTGTGTTTGTGGGAAATGTTCATGTCACAAAATTAAGCTTGGAAAA
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       GTGTATGGAAAATACTGTGAAAAGGATGACTTTTCTTGTCCATATCACCATGGAAATCTG
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       TGTGCTGGGCATGGAGAGTGTGAAGCAGGCAGATGCCAATGCTTCAGTGGCTGGGAAGGT
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       GATCGATGCCAGTGCCCTTCAGCAGCAGCCCAGCACTGTGTCAATTCAAAGGGCCAAGTG
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       TGCAGTGGAAGAGGCACGTGTGTGTGTGGAAGGTGTGAGTGCACCGATCCCAGGAGCATC
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       GGCCGCTTCTGTGAACACTGCCCCACCTGTTATACAGCCTGCAAGGAAAACTGGAATTGT
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       ATGCAATGCCTTCACCCTCACAATTTGTCTCAGGCTATACTTGATCAGTGCAAAACCTCA
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       TGTGCTCTCATGGAACAACAGCATTATGTCGACCAAACTTCAGAATGTTTCTCCAGCCCA
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       AGCTACTTGAGAATATTTTTCATCATTTTCATAGTTACATTCTTGATTGGGTTGCTTAAA
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       GTCCTGATCATTAGACAGGTGATACTACAATGGAATAGTAATAAAATTAAGTCCTCATCA
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       GATTACAGAGTGTCAGCCTCAAAAAAGGATAAGTTGATTCTGCAAAGTGTTTGCACAAGA
                                                                              

HsCD00511912      ------------------------------------------------------------
NM_002214.2       GCAGTCACCTACCGACGTGAGAAGCCTGAAGAAATAAAAATGGATATCAGCAAATTAAAT
                                                                              

HsCD00511912      ------------------------------
NM_002214.2       GCTCATGAAACTTTCAGGTGCAACTTCTAA
                                                

Pathways

NCI : Beta5 beta6 beta7 and beta8 integrin cell surface interactions
NCI : Integrin family cell surface interactions
Panther : Integrin signalling pathway
Reactome : Elastic fibre formation
Reactome : Extracellular matrix organization
Reactome : Integrin cell surface interactions
Reactome : Molecules associated with elastic fibres
WikiPathway : Focal Adhesion
WikiPathway : Integrin-mediated cell adhesion

Clone

Cloning Information : 669456
Original Clone ID : ccsbBroadEn_10927

Organism


TAX_ID : 9606
Species Specific ID: 3696

Genome

Chromosome : 7
Map Location : 7p21.1
Ensembl : ENSG00000105855

Reagents

Labome : ITGB8-antibody