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PLTP (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PLTP
Gene Name:  phospholipid transfer protein
Sequence             
Original Clone ID: ccsbBroadEn_01224
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC005045.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00511916

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1324nts         Open reading frame : 1 to 1324
Reference Sequence Alignment

ATGGCCCTCTTCGGGGCCCTCTTCCTAGCGCTGCTGGCAGGCGCACATGCAGAGTTCCCA
GGCTGCAAGATCCGCGTCACCTCCAAGGCGCTGGAGCTGGTGAAGCAGGAGGGGCTGCGC
TTTCTGGAGCAAGAGCTGGAGACTATCACCATTCCGGACCTGCGGGGCAAAGAAGGCCAC
TTCTACTACAACATCTCTGAGGTGAAGGTCACAGAGCTGCAACTGACATCTTCCGAGCTC
GATTTCCAGCCACAGCAGGAGCTGATGCTTCAAATCACCAATGCCTCCTTGGGGCTGCGC
TTCCGGAGACAGCTGCTCTACTGGTTCTTGAAGGTGTATGATTTTCTCTCCACGTTCATC
ACCTCAGGGATGCGCTTCCTCCTCAACCAGCAGATCTGCCCTGTCCTCTACCACGCAGGG
ACGGTCCTGCTCAACTCCCTCCTGGACACCGTGCCTGTGCGCAGTTCTGTGGACGAGCTT
GTTGGCATTGACTATTCCCTCATGAAGGATCCTGTGGCTTCCACCAGCAACCTGGACATG
GACTTCCGGGGGGCCTTCTTCCCCCTGACTGAGAGGAACTGGAGCCTCCCCAACCGGGCA
GTGGAGCCCCAGCTGCAGGAGGAAGAGCGGATGGTGTATGTGGCCTTCTCTGAGTTCTTC
TTCGACTCTGCCATGGAGAGCTACTTCCGGGCGGGGGCCCTGCAGCTGTTGCTGGTGGGG
GACAAGGTGCCCCACGACCTGGACATGCTGCTGAGGGCCACCTACTTTGGGAGCATTGTC
CTGCTGAGCCCAGCAGTGATTGACTCCCCATTGAAGCTGGAGCTGCGGGTCCTGGCCCCA
CCGCGCTGCACCATCAAGCCCTCTGGCACCACCATCTCTGTCACTGCTAGCGTCACCATT
GCCCTGGTCCCACCAGACCAGCCTGAGGTCCAGCTGTCCAGCATGACTATGGACGCCCGT
CTCAGCGCCAAGATGGCTCTCCGGGGGAAGGCCCTGCGCACGCAGCTGGACCTGCGCAGG
TTCCGAATCTATTCCAACCATTCTGCACTGGAGTCGCTGGCTCTGATCCCATTACAGGCC
CCTCTGAAGACCATGCTGCAGATTGGGGTGATGCCCATGCTCAATGAGCGGACCTGGCGT
GGGGTGCAGATCCCACTACCTGAGGGCATCAACTTTGTGCATGAGGTGGTGACGAACCAT
GCGGGATTCCTCACCATCGGGGCTGATCTCCACTTTGCCAAAGGGCTGCGAGAGGTGATT
GAGAAGAACCGGCCTGCTGATGTCAGGGCGTCCACTGCCCCCACACCGTCCACAGCAGCT
GTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PLTP
Symbol Nomenclature : PLTP
SYNONYM : BPIFE; HDLCQ9
Designation : BPI fold containing family E|lipid transfer protein II
Full Nomenclature : phospholipid transfer protein
GENEID : 5360
GenBank Accession : DQ894981
HGNC : 9093
MIM : 172425
Vega : OTTHUMG00000033047
Target GenBank: DQ894981

 

Reference Sequence Alignment

  

HsCD00511916      ATGGCCCTCTTCGGGGCCCTCTTCCTAGCGCTGCTGGCAGGCGCACATGCAGAGTTCCCA
NM_182676.2       ATGGCCCTCTTCGGGGCCCTCTTCCTAGCGCTGCTGGCAGGCGCACATGCAGAGTTCCCA
                  ************************************************************

HsCD00511916      GGCTGCAAGATCCGCGTCACCTCCAAGGCGCTGGAGCTGGTGAAGCAGGAGGGGCTGCGC
NM_182676.2       GGCTGCAAGATCCGCGTCACCTCCAAGGCGCTGGAGCTGGTGAAGCAGGAGGGGCTGCGC
                  ************************************************************

HsCD00511916      TTTCTGGAGCAAGAGCTGGAGACTATCACCATTCCGGACCTGCGGGGCAAAGAAGGCCAC
NM_182676.2       TTTCTGGAGCAAGAGCTGGAGACTATCACCATTCCGGACCTGCGGGGCAAAGAAGGCCAC
                  ************************************************************

HsCD00511916      TTCTACTACAACATCTCTGAGGTGAAGGTCACAGAGCTGCAACTGACATCTTCCGAGCTC
NM_182676.2       TTCTACTACAACATCTCTGAGGTGAAGGTCACAGAGCTGCAACTGACATCTTCCGAGCTC
                  ************************************************************

HsCD00511916      GATTTCCAGCCACAGCAGGAGCTGATGCTTCAAATCACCAATGCCTCCTTGGGGCTGCGC
NM_182676.2       GATTTCCAGCCACAGCAGGAGCTGATGCTTCAAATCACCAATGCCTCCTTGGGGCTGCGC
                  ************************************************************

HsCD00511916      TTCCGGAGACAGCTGCTCTACTGGTTCTTGAAGGTGTATGATTTTCTCTCCACGTTCATC
NM_182676.2       TTCCGGAGACAGCTGCTCTACTGGTTCTTGAAGGTGTATGATTTTCTCTCCACGTTCATC
                  ************************************************************

HsCD00511916      ACCTCAGGGATGCGCTTCCTCCTCAACCAGCAGATCTGCCCTGTCCTCTACCACGCAGGG
NM_182676.2       ACCTCAGGGATGCGCTTCCTCCTCAACCAGCAGATCTGCCCTGTCCTCTACCACGCAGGG
                  ************************************************************

HsCD00511916      ACGGTCCTGCTCAACTCCCTCCTGGACACCGTGCCTGTGCGCAGTTCTGTGGACGAGCTT
NM_182676.2       ACGGTCCTGCTCAACTCCCTCCTGGACACCGTGCCTGTGCGCAGTTCTGTGGACGAGCTT
                  ************************************************************

HsCD00511916      GTTGGCATTGACTATTCCCTCATGAAGGATCCTGTGGCTTCCACCAGCAACCTGGACATG
NM_182676.2       GTTGGCATTGACTATTCCCTCATGAAGGATCCTGTGGCTTCCACCAGCAACCTGGACATG
                  ************************************************************

HsCD00511916      GACTTCCGGGGGGCCTTCTTCCCCCTGACTGAGAGGAACTGGAGCCTCCCCAACCGGGCA
NM_182676.2       GACTTCCGGGGGGCCTTCTTCCCCCTGACTGAGAGGAACTGGAGCCTCCCCAACCGGGCA
                  ************************************************************

HsCD00511916      GTGGAGCCCCAGCTGCAGGAGGAAGAGCGGATGGTGTATGTGGCCTTCTCTGAGTTCTTC
NM_182676.2       GTGGAGCCCCAGCTGCAGGAGGAAGAGCGGATGGTGTATGTGGCCTTCTCTGAGTTCTTC
                  ************************************************************

HsCD00511916      TTCGACTCTGCCATGGAGAGCTACTTCCGGGCGGGGGCCCTGCAGCTGTTGCTGGTGGGG
NM_182676.2       TTCGACTCTGCCATGGAGAGCTACTTCCGGGCGGGGGCCCTGCAGCTGTTGCTGGTGGGG
                  ************************************************************

HsCD00511916      GACAAGGTGCCCCACGACCTGGACATGCTGCTGAGGGCCACCTACTTTGGGAGCATTGTC
NM_182676.2       GACAAGGTGCCCCACGACCTGGACATGCTGCTGAGGGCCACCTACTTTGGGAGCATTGTC
                  ************************************************************

HsCD00511916      CTGCTGAGCCCAGCAGTGATTGACTCCCCATTGAAGCTGGAGCTGCGGGTCCTGGCCCCA
NM_182676.2       CTGCTGAGCCCAGCAGTGATTGACTCCCCATTGAAGCTGGAGCTGCGGGTCCTGGCCCCA
                  ************************************************************

HsCD00511916      CCGCGCTGCACCATCAAGCCCTCTGGCACCACCATCTCTGTCACTGCTAGCGTCACCATT
NM_182676.2       CCGCGCTGCACCATCAAGCCCTCTGGCACCACCATCTCTGTCACTGCTAGCGTCACCATT
                  ************************************************************

HsCD00511916      GCCCTGGTCCCACCAGACCAGCCTGAGGTCCAGCTGTCCAGCATGACTATGGACGCCCGT
NM_182676.2       GCCCTGGTCCCACCAGACCAGCCTGAGGTCCAGCTGTCCAGCATGACTATGGACGCCCGT
                  ************************************************************

HsCD00511916      CTCAGCGCCAAGATGGCTCTCCGGGGGAAGGCCCTGCGCACGCAGCTGGACCTGCGCAGG
NM_182676.2       CTCAGCGCCAAGATGGCTCTCCGGGGGAAGGCCCTGCGCACGCAGCTGGACCTGCGCAGG
                  ************************************************************

HsCD00511916      TTCCGAATCTATTCCAACCATTCTGCACTGGAGTCGCTGGCTCTGATCCCATTACAGGCC
NM_182676.2       TTCCGAATCTATTCCAACCATTCTGCACTGGAGTCGCTGGCTCTGATCCCATTACAGGCC
                  ************************************************************

HsCD00511916      CCTCTGAAGACCATGCTGCAGATTGGGGTGATGCCCATGCTCAATGAGCGGACCTGGCGT
NM_182676.2       CCTCTGAAGACCATGCTGCAGATTGGGGTGATGCCCATGCTCAATGAGCGGACCTGGCGT
                  ************************************************************

HsCD00511916      GGGGTGCAGATCCCACTACCTGAGGGCATCAACTTTGTGCATGAGGTGGTGACGAACCAT
NM_182676.2       GGGGTGCAGATCCCACTACCTGAGGGCATCAACTTTGTGCATGAGGTGGTGACGAACCAT
                  ************************************************************

HsCD00511916      GCGGGATTCCTCACCATCGGGGCTGATCTCCACTTTGCCAAAGGGCTGCGAGAGGTGATT
NM_182676.2       GCGGGATTCCTCACCATCGGGGCTGATCTCCACTTTGCCAAAGGGCTGCGAGAGGTGATT
                  ************************************************************

HsCD00511916      GAGAAGAACCGGCCTGCTGATGTCAGGGCGTCCACTGCCCCCACACCGTCCACAGCAGCT
NM_182676.2       GAGAAGAACCGGCCTGCTGATGTCAGGGCGTCCACTGCCCCCACACCGTCCACAGCAGCT
                  ************************************************************

HsCD00511916      GTC---
NM_182676.2       GTCTGA
                  ***   

Pathways

Reactome : HDL-mediated lipid transport
Reactome : Lipid digestion, mobilization, and transport
Reactome : Lipoprotein metabolism
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
WikiPathway : Statin Pathway

Clone

Cloning Information : 604845
Original Clone ID : ccsbBroadEn_01224

Organism


TAX_ID : 9606
Species Specific ID: 5360

Genome

Chromosome : 20
Map Location : 20q13.12
Ensembl : ENSG00000100979