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TIMM44 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  TIMM44
Gene Name:  translocase of inner mitochondrial membrane 44 homolog (yeast)
Sequence             
Original Clone ID: ccsbBroadEn_02445
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC033628.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00512100

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1357nts         Open reading frame : 1 to 1357
Reference Sequence Alignment

ATGGCGGCGGCGGCCCTGCGGAGTGGCTGGTGCCGCTGTCCACGGAGATGCCTCGGCAGT
GGAATCCAATTTCTTTCCAGCCACAACCTACCCCATGGGTCGACCTATCAGATGCGCCGG
CCGGGCGGAGAGCTGCCACTGTCCAAATCATATTCTTCTGGAAACAGAAAAGGCTTTCTG
TCCGGCTTGCTAGATAATGTCAAACAAGAATTAGCCAAAAACAAAGAAATGAAAGAAAGT
ATAAAAAAATTCCGTGACGAGGCCAGAAGGCTAGAAGAATCAGACGTGCTCCAGGAGGCC
AGAAGGAAATACAAAACCATCGAGTCAGAAACCGTGCGGACGAGCGAGGTGCTACGGAAG
AAGCTTGGGGAGCTGACGGGCACCGTGAAGGAGAGCCTTCACGAAGTCAGTAAAAGTGAT
CTCGGCCGGAAAATCAAGGAGGGCGTGGAGGAAGCAGCCAAGACGGCCAAGCAGTCGGCC
GAGTCGGTATCCAAAGGCGGGGAGAAGCTGGGCAGGACAGCGGCCTTCAGAGCCCTCTCC
CAGGGGGTGGAGTCCGTGAAGAAGGAAATTGACGACAGCGTCCTGGGACAGACCGGGCCC
TACCGGAGGCCCCAGCGACTCCGGAAGAGAACGGAGTTTGCGGGAGATAAGTTCAAGGAG
GAGAAAGTGTTTGAGCCAAACGAGGAGGCCCTGGGGGTCGTGCTGCACAAGGACTCCAAG
TGGTACCAGCAGTGGAAGGACTTCAAGGAGAACAACGTGGTGTTTAACCGGTTCTTCGAG
ATGAAGATGAAGTATGACGAAAGCGACAACGCGTTCATCCGGGCATCCCGGGCCCTTACG
GACAAGGTCACCGACTTGCTGGGGGGCCTGTTCTCCAAGACAGAGATGTCGGAGGTGCTC
ACGGAGATCCTCCGGGTGGACCCGGCCTTTGACAAGGACCGGTTTCTGAAACAGTGCGAG
AACGACATCATCCCCAATGTCCTGGAGGCCATGATTTCTGGAGAGCTTGACATTCTCAAA
GACTGGTGCTATGAAGCTACTTACAGCCAGCTGGCCCACCCCATCCAGCAGGCCAAGGCA
CTGGGTCTCCAGTTCCATTCTCGCATCCTAGACATTGACAACGTCGACCTGGCCATGGGC
AAGATGATGGAGCAGGGGCCGGTGCTGATCATCACCTTCCAGGCACAGCTGGTGATGGTG
GTCAGGAACCCCAAAGGCGAGGTGGTGGAGGGTGACCCGGACAAGGTGCTGCGGATGCTG
TACGTGTGGGCGCTCTGCCGAGACCAGGACGAGCTCAACCCCTACGCGGCCTGGCGGCTC
CTGGACATCTCGGCCTCCAGCACCGAGCAGATTCTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : TIMM44
Symbol Nomenclature : TIMM44
SYNONYM : TIM44
Designation : mitochondrial import inner membrane translocase subunit TIM44|mitochondrial inner membrane translocase
Full Nomenclature : translocase of inner mitochondrial membrane 44 homolog (yeast)
GENEID : 10469
GenBank Accession : DQ894486
HGNC : 17316
MIM : 605058
Vega : OTTHUMG00000182478
Target GenBank: DQ894486

 

Reference Sequence Alignment

  

HsCD00512100      ATGGCGGCGGCGGCCCTGCGGAGTGGCTGGTGCCGCTGTCCACGGAGATGCCTCGGCAGT
NM_006351.3       ATGGCGGCGGCGGCCCTGCGGAGTGGCTGGTGCCGCTGTCCACGGAGATGCCTCGGCAGT
                  ************************************************************

HsCD00512100      GGAATCCAATTTCTTTCCAGCCACAACCTACCCCATGGGTCGACCTATCAGATGCGCCGG
NM_006351.3       GGAATCCAATTTCTTTCCAGCCACAACCTACCCCATGGGTCGACCTATCAGATGCGCCGG
                  ************************************************************

HsCD00512100      CCGGGCGGAGAGCTGCCACTGTCCAAATCATATTCTTCTGGAAACAGAAAAGGCTTTCTG
NM_006351.3       CCGGGCGGAGAGCTGCCACTGTCCAAATCATATTCTTCTGGAAACAGAAAAGGCTTTCTG
                  ************************************************************

HsCD00512100      TCCGGCTTGCTAGATAATGTCAAACAAGAATTAGCCAAAAACAAAGAAATGAAAGAAAGT
NM_006351.3       TCCGGCTTGCTAGATAATGTCAAACAAGAATTAGCCAAAAACAAAGAAATGAAAGAAAGT
                  ************************************************************

HsCD00512100      ATAAAAAAATTCCGTGACGAGGCCAGAAGGCTAGAAGAATCAGACGTGCTCCAGGAGGCC
NM_006351.3       ATAAAAAAATTCCGTGACGAGGCCAGAAGGCTAGAAGAATCAGACGTGCTCCAGGAGGCC
                  ************************************************************

HsCD00512100      AGAAGGAAATACAAAACCATCGAGTCAGAAACCGTGCGGACGAGCGAGGTGCTACGGAAG
NM_006351.3       AGAAGGAAATACAAAACCATCGAGTCAGAAACCGTGCGGACGAGCGAGGTGCTACGGAAG
                  ************************************************************

HsCD00512100      AAGCTTGGGGAGCTGACGGGCACCGTGAAGGAGAGCCTTCACGAAGTCAGTAAAAGTGAT
NM_006351.3       AAGCTTGGGGAGCTGACGGGCACCGTGAAGGAGAGCCTTCACGAAGTCAGTAAAAGTGAT
                  ************************************************************

HsCD00512100      CTCGGCCGGAAAATCAAGGAGGGCGTGGAGGAAGCAGCCAAGACGGCCAAGCAGTCGGCC
NM_006351.3       CTCGGCCGGAAAATCAAGGAGGGCGTGGAGGAAGCAGCCAAGACGGCCAAGCAGTCGGCC
                  ************************************************************

HsCD00512100      GAGTCGGTATCCAAAGGCGGGGAGAAGCTGGGCAGGACAGCGGCCTTCAGAGCCCTCTCC
NM_006351.3       GAGTCGGTATCCAAAGGCGGGGAGAAGCTGGGCAGGACAGCGGCCTTCAGAGCCCTCTCC
                  ************************************************************

HsCD00512100      CAGGGGGTGGAGTCCGTGAAGAAGGAAATTGACGACAGCGTCCTGGGACAGACCGGGCCC
NM_006351.3       CAGGGGGTGGAGTCCGTGAAGAAGGAAATTGACGACAGCGTCCTGGGACAGACCGGGCCC
                  ************************************************************

HsCD00512100      TACCGGAGGCCCCAGCGACTCCGGAAGAGAACGGAGTTTGCGGGAGATAAGTTCAAGGAG
NM_006351.3       TACCGGAGGCCCCAGCGACTCCGGAAGAGAACGGAGTTTGCGGGAGATAAGTTCAAGGAG
                  ************************************************************

HsCD00512100      GAGAAAGTGTTTGAGCCAAACGAGGAGGCCCTGGGGGTCGTGCTGCACAAGGACTCCAAG
NM_006351.3       GAGAAAGTGTTTGAGCCAAACGAGGAGGCCCTGGGGGTCGTGCTGCACAAGGACTCCAAG
                  ************************************************************

HsCD00512100      TGGTACCAGCAGTGGAAGGACTTCAAGGAGAACAACGTGGTGTTTAACCGGTTCTTCGAG
NM_006351.3       TGGTACCAGCAGTGGAAGGACTTCAAGGAGAACAACGTGGTGTTTAACCGGTTCTTCGAG
                  ************************************************************

HsCD00512100      ATGAAGATGAAGTATGACGAAAGCGACAACGCGTTCATCCGGGCATCCCGGGCCCTTACG
NM_006351.3       ATGAAGATGAAGTATGACGAAAGCGACAACGCGTTCATCCGGGCATCCCGGGCCCTTACG
                  ************************************************************

HsCD00512100      GACAAGGTCACCGACTTGCTGGGGGGCCTGTTCTCCAAGACAGAGATGTCGGAGGTGCTC
NM_006351.3       GACAAGGTCACCGACTTGCTGGGGGGCCTGTTCTCCAAGACAGAGATGTCGGAGGTGCTC
                  ************************************************************

HsCD00512100      ACGGAGATCCTCCGGGTGGACCCGGCCTTTGACAAGGACCGGTTTCTGAAACAGTGCGAG
NM_006351.3       ACGGAGATCCTCCGGGTGGACCCGGCCTTTGACAAGGACCGGTTTCTGAAACAGTGCGAG
                  ************************************************************

HsCD00512100      AACGACATCATCCCCAATGTCCTGGAGGCCATGATTTCTGGAGAGCTTGACATTCTCAAA
NM_006351.3       AACGACATCATCCCCAATGTCCTGGAGGCCATGATTTCTGGAGAGCTTGACATTCTCAAA
                  ************************************************************

HsCD00512100      GACTGGTGCTATGAAGCTACTTACAGCCAGCTGGCCCACCCCATCCAGCAGGCCAAGGCA
NM_006351.3       GACTGGTGCTATGAAGCTACTTACAGCCAGCTGGCCCACCCCATCCAGCAGGCCAAGGCA
                  ************************************************************

HsCD00512100      CTGGGTCTCCAGTTCCATTCTCGCATCCTAGACATTGACAACGTCGACCTGGCCATGGGC
NM_006351.3       CTGGGTCTCCAGTTCCATTCTCGCATCCTAGACATTGACAACGTCGACCTGGCCATGGGC
                  ************************************************************

HsCD00512100      AAGATGATGGAGCAGGGGCCGGTGCTGATCATCACCTTCCAGGCACAGCTGGTGATGGTG
NM_006351.3       AAGATGATGGAGCAGGGGCCGGTGCTGATCATCACCTTCCAGGCACAGCTGGTGATGGTG
                  ************************************************************

HsCD00512100      GTCAGGAACCCCAAAGGCGAGGTGGTGGAGGGTGACCCGGACAAGGTGCTGCGGATGCTG
NM_006351.3       GTCAGGAACCCCAAAGGCGAGGTGGTGGAGGGTGACCCGGACAAGGTGCTGCGGATGCTG
                  ************************************************************

HsCD00512100      TACGTGTGGGCGCTCTGCCGAGACCAGGACGAGCTCAACCCCTACGCGGCCTGGCGGCTC
NM_006351.3       TACGTGTGGGCGCTCTGCCGAGACCAGGACGAGCTCAACCCCTACGCGGCCTGGCGGCTC
                  ************************************************************

HsCD00512100      CTGGACATCTCGGCCTCCAGCACCGAGCAGATTCTC---
NM_006351.3       CTGGACATCTCGGCCTCCAGCACCGAGCAGATTCTCTGA
                  ************************************   

Pathways

Reactome : Metabolism of proteins
Reactome : Mitochondrial protein import

Protein

SMART domain : Tim44 :
HPRD : 05450

Clone

Cloning Information : 604654
Original Clone ID : ccsbBroadEn_02445

Organism


TAX_ID : 9606
Species Specific ID: 10469

Genome

Chromosome : 19
Map Location : 19p13.3-p13.2
Ensembl : ENSG00000104980

Reagents

Labome : TIMM44-antibody