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C20orf185 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  BPIFB3
Gene Name:  BPI fold containing family B, member 3
Sequence             
Original Clone ID: ccsbBroadEn_10063
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC113398.1,BC113400.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00512273

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1429nts         Open reading frame : 1 to 1429
Reference Sequence Alignment

ATGCAGCCAGTCATGCTGGCCCTGTGGTCCCTGCTTCTGCTCTGGGGCCTGGCGACTCCA
TGCCAGGAGCTGCTAGAGACGGTGGGCACGCTCGCTCGGATTGACAAGGATGAACTCGGC
AAAGCCATCCAGAACTCACTGGTTGGGGAGCCCATTCTGCAGAATGTGCTGGGATCGGTC
ACAGCTGTGAACCGGGGCCTCTTGGGCTCAGGAGGGCTGCTTGGAGGAGGCGGCTTGCTG
GGCCACGGAGGGGTTTTTGGCGTTGTCGAGGAGCTCTCTGGTCTGAAGATTGAGGAGCTC
ACGCTGCCAAAGGTGTTGCTGAAGCTGCTGCCGGGATTTGGGGTGCAGCTGAGCCTGCAC
ACCAAAGTGGGCATGCATTGCTCTGGCCCCCTTGGTGGCCTTCTGCAGCTGGCTGCGGAG
GTGAACGTGACATCGCGGGTGGCGCTGGCCGTGAGCTCAAGGGGCACACCCATCCTTATC
CTCAAGCGCTGCAGCACGCTCCTGGGCCACATCAGCCTGTTCTCAGGGCTGCTGCCCACA
CCACTCTTTGGGGTCGTGGAACAGATGCTCTTCAAGGTGCTTCCGGGACTGCTGTGCCCC
GTGGTGGACAGTGTGCTGGGTGTGGTGAATGAGCTCCTGGGGGCTGTGCTGGGCCTGGTG
TCCCTTGGGGCTCTTGGGTCCGTGGAATTCTCTCTGGCCACATTGCCTCTCATCTCCAAC
CAGTACATAGAACTGGACATCAACCCTATCGTGAAGAGTGTAGCTGGTGATATCATTGAC
TTCCCCAAGTCCCGTGCCCCAGCCAAGGTGCCCCCCAAGAAGGACCACACATCCCAGGTG
ATGGTGCCACTGTACCTCTTCAACACCATGTTTGGACTCCTGCAGACCAACGGCGCCCTC
GACATGGACATCACCCCTGAGCTGGTTCCCAGCGATGTCCCACTGACAACTACAGACCTG
GCAGCTTTGCTCCCTGAGGCCCTGGGGAAGCTGCCCCTGCACCAGCAACTCCTACTGTTC
CTGCGGGTGAGGGAAGCTCCCACGGTCACACTCCACAACAAGAAGGCCTTGGTCTCCCTC
CCAGCCAACATCCATGTGCTGTTCTATGTCCCTAAGGGGACCCCTGAATCCCTCTTTGAG
CTGAACTCCGTCATGACTGTGCGTGCCCAGCTGGCTCCCTCGGCTACCAAGCTGCACATC
TCCCTGTCCCTGGAACGGCTCAGTGTCAAGGTGGCCTCCTCCTTTACCCATGCCTTTGAC
GGATCGCGTTTAGAAGAATGGCTCAGCCATGTGGTCGGGGCAGTGTATGCACCAAAGCTT
AACGTGGCCCTGGATGTTGGAATTTCCCTGCCTAAGGTTCTTAATATCAATTTTTCCAAT
TCAGTTCTGGAGATCGTAGAGAATGCTGTTGTGCTGACCGTGGCATCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : BPIFB3
Symbol Nomenclature : BPIFB3
SYNONYM : C20orf185; LPLUNC3; RYA3; dJ726C3.4
Designation : BPI fold-containing family B member 3|ligand-binding protein RYA3|long palate, lung and nasal epithelium carcinoma associated 3|long palate, lung and nasal epithelium carcinoma-associated protein 3
Full Nomenclature : BPI fold containing family B, member 3
GENEID : 359710
GenBank Accession : HQ258440
HGNC : 16178
Vega : OTTHUMG00000032234
Target GenBank: HQ258440

 

Reference Sequence Alignment

  

HsCD00512273      ATGCAGCCAGTCATGCTGGCCCTGTGGTCCCTGCTTCTGCTCTGGGGCCTGGCGACTCCA
NM_182658.1       ATGCAGCCAGTCATGCTGGCCCTGTGGTCCCTGCTTCTGCTCTGGGGCCTGGCGACTCCA
                  ************************************************************

HsCD00512273      TGCCAGGAGCTGCTAGAGACGGTGGGCACGCTCGCTCGGATTGACAAGGATGAACTCGGC
NM_182658.1       TGCCAGGAGCTGCTAGAGACGGTGGGCACGCTCGCTCGGATTGACAAGGATGAACTCGGC
                  ************************************************************

HsCD00512273      AAAGCCATCCAGAACTCACTGGTTGGGGAGCCCATTCTGCAGAATGTGCTGGGATCGGTC
NM_182658.1       AAAGCCATCCAGAACTCACTGGTTGGGGAGCCCATTCTGCAGAATGTGCTGGGATCGGTC
                  ************************************************************

HsCD00512273      ACAGCTGTGAACCGGGGCCTCTTGGGCTCAGGAGGGCTGCTTGGAGGAGGCGGCTTGCTG
NM_182658.1       ACAGCTGTGAACCGGGGCCTCTTGGGCTCAGGAGGGCTGCTTGGAGGAGGCGGCTTGCTG
                  ************************************************************

HsCD00512273      GGCCACGGAGGGGTTTTTGGCGTTGTCGAGGAGCTCTCTGGTCTGAAGATTGAGGAGCTC
NM_182658.1       GGCCACGGAGGGGTTTTTGGCGTTGTCGAGGAGCTCTCTGGTCTGAAGATTGAGGAGCTC
                  ************************************************************

HsCD00512273      ACGCTGCCAAAGGTGTTGCTGAAGCTGCTGCCGGGATTTGGGGTGCAGCTGAGCCTGCAC
NM_182658.1       ACGCTGCCAAAGGTGTTGCTGAAGCTGCTGCCGGGATTTGGGGTGCAGCTGAGCCTGCAC
                  ************************************************************

HsCD00512273      ACCAAAGTGGGCATGCATTGCTCTGGCCCCCTTGGTGGCCTTCTGCAGCTGGCTGCGGAG
NM_182658.1       ACCAAAGTGGGCATGCATTGCTCCGGCCCCCTTGGTGGCCTTCTGCAGCTGGCTGCGGAG
                  *********************** ************************************

HsCD00512273      GTGAACGTGACATCGCGGGTGGCGCTGGCCGTGAGCTCAAGGGGCACACCCATCCTTATC
NM_182658.1       GTGAACGTGACATCGCGGGTGGCGCTGGCCGTGAGCTCAAGGGGCACACCCATCCTTATC
                  ************************************************************

HsCD00512273      CTCAAGCGCTGCAGCACGCTCCTGGGCCACATCAGCCTGTTCTCAGGGCTGCTGCCCACA
NM_182658.1       CTCAAGCGCTGCAGCACGCTCCTGGGCCACATCAGCCTGTTCTCAGGGCTGCTGCCCACA
                  ************************************************************

HsCD00512273      CCACTCTTTGGGGTCGTGGAACAGATGCTCTTCAAGGTGCTTCCGGGACTGCTGTGCCCC
NM_182658.1       CCACTCTTTGGGGTCGTGGAACAGATGCTCTTCAAGGTGCTTCCGGGACTGCTGTGCCCC
                  ************************************************************

HsCD00512273      GTGGTGGACAGTGTGCTGGGTGTGGTGAATGAGCTCCTGGGGGCTGTGCTGGGCCTGGTG
NM_182658.1       GTGGTGGACAGTGTGCTGGGTGTGGTGAATGAGCTCCTGGGGGCTGTGCTGGGCCTGGTG
                  ************************************************************

HsCD00512273      TCCCTTGGGGCTCTTGGGTCCGTGGAATTCTCTCTGGCCACATTGCCTCTCATCTCCAAC
NM_182658.1       TCCCTTGGGGCTCTTGGGTCCGTGGAATTCTCTCTGGCCACATTGCCTCTCATCTCCAAC
                  ************************************************************

HsCD00512273      CAGTACATAGAACTGGACATCAACCCTATCGTGAAGAGTGTAGCTGGTGATATCATTGAC
NM_182658.1       CAGTACATAGAACTGGACATCAACCCTATCGTGAAGAGTGTAGCTGGTGATATCATTGAC
                  ************************************************************

HsCD00512273      TTCCCCAAGTCCCGTGCCCCAGCCAAGGTGCCCCCCAAGAAGGACCACACATCCCAGGTG
NM_182658.1       TTCCCCAAGTCCCGTGCCCCAGCCAAGGTGCCCCCCAAGAAGGACCACACATCCCAGGTG
                  ************************************************************

HsCD00512273      ATGGTGCCACTGTACCTCTTCAACACCATGTTTGGACTCCTGCAGACCAACGGCGCCCTC
NM_182658.1       ATGGTGCCACTGTACCTCTTCAACACCACGTTTGGACTCCTGCAGACCAACGGCGCCCTC
                  **************************** *******************************

HsCD00512273      GACATGGACATCACCCCTGAGCTGGTTCCCAGCGATGTCCCACTGACAACTACAGACCTG
NM_182658.1       GACATGGACATCACCCCTGAGCTGGTTCCCAGCGATGTCCCACTGACAACTACAGACCTG
                  ************************************************************

HsCD00512273      GCAGCTTTGCTCCCTGAGGCCCTGGGGAAGCTGCCCCTGCACCAGCAACTCCTACTGTTC
NM_182658.1       GCAGCTTTGCTCCCTGAGGCCCTGGGGAAGCTGCCCCTGCACCAGCAACTCCTACTGTTC
                  ************************************************************

HsCD00512273      CTGCGGGTGAGGGAAGCTCCCACGGTCACACTCCACAACAAGAAGGCCTTGGTCTCCCTC
NM_182658.1       CTGCGGGTGAGGGAAGCTCCCACGGTCACACTCCACAACAAGAAGGCCTTGGTCTCCCTC
                  ************************************************************

HsCD00512273      CCAGCCAACATCCATGTGCTGTTCTATGTCCCTAAGGGGACCCCTGAATCCCTCTTTGAG
NM_182658.1       CCAGCCAACATCCATGTGCTGTTCTATGTCCCTAAGGGGACCCCTGAATCCCTCTTTGAG
                  ************************************************************

HsCD00512273      CTGAACTCCGTCATGACTGTGCGTGCCCAGCTGGCTCCCTCGGCTACCAAGCTGCACATC
NM_182658.1       CTGAACTCCGTCATGACTGTGCGTGCCCAGCTGGCTCCCTCGGCTACCAAGCTGCACATC
                  ************************************************************

HsCD00512273      TCCCTGTCCCTGGAACGGCTCAGTGTCAAGGTGGCCTCCTCCTTTACCCATGCCTTTGAC
NM_182658.1       TCCCTGTCCCTGGAACGGCTCAGTGTCAAGGTGGCCTCCTCCTTTACCCATGCCTTTGAC
                  ************************************************************

HsCD00512273      GGATCGCGTTTAGAAGAATGGCTCAGCCATGTGGTCGGGGCAGTGTATGCACCAAAGCTT
NM_182658.1       GGATCGCGTTTAGAAGAATGGCTCAGCCATGTGGTCGGGGCAGTGTATGCACCAAAGCTT
                  ************************************************************

HsCD00512273      AACGTGGCCCTGGATGTTGGAATTTCCCTGCCTAAGGTTCTTAATATCAATTTTTCCAAT
NM_182658.1       AACGTGGCCCTGGATGTTGGAATTCCCCTGCCTAAGGTTCTTAATATCAATTTTTCCAAT
                  ************************ ***********************************

HsCD00512273      TCAGTTCTGGAGATCGTAGAGAATGCTGTTGTGCTGACCGTGGCATCC---
NM_182658.1       TCAGTTCTGGAGATCGTAGAGAATGCTGTTGTGCTGACCGTGGCATCCTGA
                  ************************************************   

Clone

Cloning Information : 604452
Original Clone ID : ccsbBroadEn_10063

Organism


TAX_ID : 9606
Species Specific ID: 359710

Genome

Chromosome : 20
Map Location : 20q11.21
Ensembl : ENSG00000186190

Reagents

Labome : BPIFB3-antibody