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KLHDC7B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHDC7B
Gene Name:  kelch domain containing 7B
Sequence             
Original Clone ID: ccsbBroadEn_13026
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC009980.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00512521

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1486nts         Open reading frame : 1 to 1486
Reference Sequence Alignment

ATGCCCAGGGGCCCCGCACAGCCCCCCGCGCAGAGGCCGCCTGGCCCCGCGGCCTCCTCC
TCTGCGAGGCGCTCACAGCCGGTACCCCAGCTACGGAAACGCAGCAGGTGCGAAATCGCC
CCGAGCTCGGAGCAGGAGGTCAGGCCGGCCGCCTCGGGGGACCCTCAAGGGGAGGCGCCG
GGGGAGGGGGGCAGCCCTGCCGGCCGCAGCGGGGCGCTCACGGAAAAGCAGGAGGAGGCC
CGGAAGCTCATGGTGTTTCTGCAGAGGCCCGGGGGTTGGGGGGTGGTGGAGGGGCCCCGG
AAGCCCAGCTCCCGGGCCCTGGAGCCCGCCACGGCGGCAGCCCTGCGGCGGCGGCTGGAC
CTGGGCAGTTGCCTGGACGTGCTGGCCTTTGCCCAGCAGCACGGAGAGCCCGGCCTGGCG
CAGGAGACCTACGCGCTGATGAGCGACAACCTGCTGCGAGTGCTGGGAGACCCGTGCCTC
TACCGCCGGCTGAGCGCGGCCGACCGCGAGCGCATCCTCAGCCTGCGGACCGGCCGGGGC
CGGGCGGTGCTGGGCGTCCTCGTACTGCCCAGCCTCTACCAGGGGGGCCGCTCAGGGCTC
CCCAGGGGCCCTCGTGGCGAGGAGCCTCCTGCGGCGGCCCCTGTGTCCCTGCCTCTACCT
GCGCACCTGCATGTGTTCAACCCCCGGGAGAACACCTGGCGGCCCCTGACCCAGGTGCCC
GAGGAGGCCCCGCTTCGGGGCTGCGGTCTCTGCACCATGCACAACTACCTGTTTCTGGCG
GGGGGCATCCGTGGCTCCGGTGCCAAGGCCGTCTGCTCCAACGAGGTCTTCTGCTACAAC
CCTCTGACCAACATCTGGAGCCAGGTTCGGCCCATGCAGCAGGCCCGAGCCCAGCTCAAG
CTGGTGGCCCTGGACGGGCTGCTCTATGCCATCGGTGGCGAATGCCTGTACAGCATGGAG
TGCTACGACCCGCGAACAGACGCCTGGACCCCACGCGCGCCACTCCCCGCAGGCACCTTC
CCTGTGGCCCACGAGGCTGTGGCCTGCCGTGGGGACATCTACGTCACCGGGGGTCACCTC
TTCTACCGCCTGCTCAGGTACAGCCCCGTGAAGGATGCTTGGGACGAGTGCCCATACAGT
GCCAGCCACCGGCGTTCCAGCGACATCGTGGCACTGGGGGGCTTCCTGTACCGCTTCGAC
CTGCTGCGGGGCGTGGGCGCCGCCGTGATGCGCTACAACACAGTGACCGGCTCCTGGAGC
AGGGCTGCCTCCCTGCCCCTGCCCGCCCCCGCCCCACTGCACTGCACCACCCTGGGCAAC
ACCATTTACTGCCTCAACCCCCAGGTCACTGCCACCTTCACGGTCTCTGGGGGGACTGCC
CAGTTCCAGGCCAAGGAGCTGCAGCCCTTCCCCTTGGGGAGCACCGGGGTCCTCAGTCCA
TTCATCCTGACTCTGCCCCCTGAGGACCGGCTGCAGACCTCACTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHDC7B
Symbol Nomenclature : KLHDC7B
Designation : kelch domain-containing protein 7B
Full Nomenclature : kelch domain containing 7B
GENEID : 113730
HGNC : 25145
Vega : OTTHUMG00000150250
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00512521      ------------------------------------------------------------
NM_138433.3       ATGGTTCTTAGAAGCCACCCCTTCCCCAGGCAAGACAGGCCCCAAGGGAGTGTCCCGAGG
                                                                              

HsCD00512521      ------------------------------------------------------------
NM_138433.3       GCGGTTCCCGGGAGCCCCGTGGGTCCCAGCACTTCCACACACTCTGAGGACAGACACGGC
                                                                              

HsCD00512521      ------------------------------------------------------------
NM_138433.3       CCCTCTTCTTCAGTGGGGACAGTCATAGGGACAGGTACAGGGGGCCTGGTTGAGGCTGGA
                                                                              

HsCD00512521      ------------------------------------------------------------
NM_138433.3       GGTCAGCCACAGCCAAGAAGCTCCGAGACCAACGGATCGCCCAGCCCAGACCCTCCCCCA
                                                                              

HsCD00512521      ---------------------------------------------------------ATG
NM_138433.3       GGCCTAAGAGGAGAGGGAACCAGGGAGAAAAGTCTAGACCCGCTGCCCCAAGCCGCGATG
                                                                           ***

HsCD00512521      CCCAGGGGCCCCGCACAGCCCCCCGCGCAGAGGCCGCCTGGCCCCGCGGCCTCCTCCTCT
NM_138433.3       CCCAGGGGCCCCGCACAGCCCCCCGCGCAGAGGCCGCCTGGCCCCGCGGCCTCCTCCTCT
                  ************************************************************

HsCD00512521      GCGAGGCGCTCACAGCCGGTACCCCAGCTACGGAAACGCAGCAGGTGCGAAATCGCCCCG
NM_138433.3       GCGAGGCGCTCACAGCCGGTACCCCAGCTACGGAAACGCAGCAGGTGCGAAATCGCCCCG
                  ************************************************************

HsCD00512521      AGCTCGGAGCAGGAGGTCAGGCCGGCCGCCTCGGGGGACCCTCAAGGGGAGGCGCCGGGG
NM_138433.3       AGCTCGGAGCAGGAGGTCAGGCCGGCCGCCTCGGGGGACCCTCAAGGGGAGGCGCCGGGG
                  ************************************************************

HsCD00512521      GAGGGGGGCAGCCCTGCCGGCCGCAGCGGGGCGCTCACGGAAAAGCAGGAGGAGGCCCGG
NM_138433.3       GAGGGGGGCAGCCCTGCCGGCCGCAGCGGGGCGCTCACGGAAAAGCAGGAGGAGGCCCGG
                  ************************************************************

HsCD00512521      AAGCTCATGGTGTTTCTGCAGAGGCCCGGGGGTTGGGGGGTGGTGGAGGGGCCCCGGAAG
NM_138433.3       AAGCTCATGGTGTTTCTGCAGAGGCCCGGGGGTTGGGGGGTGGTGGAGGGGCCCCGGAAG
                  ************************************************************

HsCD00512521      CCCAGCTCCCGGGCCCTGGAGCCCGCCACGGCGGCAGCCCTGCGGCGGCGGCTGGACCTG
NM_138433.3       CCCAGCTCCCGGGCCCTGGAGCCCGCCACGGCGGCAGCCCTGCGGCGGCGGCTGGACCTG
                  ************************************************************

HsCD00512521      GGCAGTTGCCTGGACGTGCTGGCCTTTGCCCAGCAGCACGGAGAGCCCGGCCTGGCGCAG
NM_138433.3       GGCAGTTGCCTGGACGTGCTGGCCTTTGCCCAGCAGCACGGAGAGCCCGGCCTGGCGCAG
                  ************************************************************

HsCD00512521      GAGACCTACGCGCTGATGAGCGACAACCTGCTGCGAGTGCTGGGAGACCCGTGCCTCTAC
NM_138433.3       GAGACCTACGCGCTGATGAGCGACAACCTGCTGCGAGTGCTGGGAGACCCGTGCCTCTAC
                  ************************************************************

HsCD00512521      CGCCGGCTGAGCGCGGCCGACCGCGAGCGCATCCTCAGCCTGCGGACCGGCCGGGGCCGG
NM_138433.3       CGCCGGCTGAGCGCGGCCGACCGCGAGCGCATCCTCAGCCTGCGGACCGGCCGGGGCCGG
                  ************************************************************

HsCD00512521      GCGGTGCTGGGCGTCCTCGTACTGCCCAGCCTCTACCAGGGGGGCCGCTCAGGGCTCCCC
NM_138433.3       GCGGTGCTGGGCGTCCTCGTACTGCCCAGCCTCTACCAGGGGGGCCGCTCAGGGCTCCCC
                  ************************************************************

HsCD00512521      AGGGGCCCTCGTGGCGAGGAGCCTCCTGCGGCGGCCCCTGTGTCCCTGCCTCTACCTGCG
NM_138433.3       AGGGGCCCTCGTGGCGAGGAGCCTCCTGCGGCGGCCCCTGTGTCCCTGCCTCTACCTGCG
                  ************************************************************

HsCD00512521      CACCTGCATGTGTTCAACCCCCGGGAGAACACCTGGCGGCCCCTGACCCAGGTGCCCGAG
NM_138433.3       CACCTGCATGTGTTCAACCCCCGGGAGAACACCTGGCGGCCCCTGACCCAGGTGCCCGAG
                  ************************************************************

HsCD00512521      GAGGCCCCGCTTCGGGGCTGCGGTCTCTGCACCATGCACAACTACCTGTTTCTGGCGGGG
NM_138433.3       GAGGCCCCGCTTCGGGGCTGCGGTCTCTGCACCATGCACAACTACCTGTTTCTGGCGGGG
                  ************************************************************

HsCD00512521      GGCATCCGTGGCTCCGGTGCCAAGGCCGTCTGCTCCAACGAGGTCTTCTGCTACAACCCT
NM_138433.3       GGCATCCGTGGCTCCGGTGCCAAGGCCGTCTGCTCCAACGAGGTCTTCTGCTACAACCCT
                  ************************************************************

HsCD00512521      CTGACCAACATCTGGAGCCAGGTTCGGCCCATGCAGCAGGCCCGAGCCCAGCTCAAGCTG
NM_138433.3       CTGACCAACATCTGGAGCCAGGTTCGGCCCATGCAGCAGGCCCGAGCCCAGCTCAAGCTG
                  ************************************************************

HsCD00512521      GTGGCCCTGGACGGGCTGCTCTATGCCATCGGTGGCGAATGCCTGTACAGCATGGAGTGC
NM_138433.3       GTGGCCCTGGACGGGCTGCTCTATGCCATCGGTGGCGAATGCCTGTACAGCATGGAGTGC
                  ************************************************************

HsCD00512521      TACGACCCGCGAACAGACGCCTGGACCCCACGCGCGCCACTCCCCGCAGGCACCTTCCCT
NM_138433.3       TACGACCCGCGAACAGACGCCTGGACCCCACGCGCGCCACTCCCCGCAGGCACCTTCCCT
                  ************************************************************

HsCD00512521      GTGGCCCACGAGGCTGTGGCCTGCCGTGGGGACATCTACGTCACCGGGGGTCACCTCTTC
NM_138433.3       GTGGCCCACGAGGCTGTGGCCTGCCGTGGGGACATCTACGTCACCGGGGGTCACCTCTTC
                  ************************************************************

HsCD00512521      TACCGCCTGCTCAGGTACAGCCCCGTGAAGGATGCTTGGGACGAGTGCCCATACAGTGCC
NM_138433.3       TACCGCCTGCTCAGGTACAGCCCCGTGAAGGATGCTTGGGACGAGTGCCCATACAGTGCC
                  ************************************************************

HsCD00512521      AGCCACCGGCGTTCCAGCGACATCGTGGCACTGGGGGGCTTCCTGTACCGCTTCGACCTG
NM_138433.3       AGCCACCGGCGTTCCAGCGACATCGTGGCACTGGGGGGCTTCCTGTACCGCTTCGACCTG
                  ************************************************************

HsCD00512521      CTGCGGGGCGTGGGCGCCGCCGTGATGCGCTACAACACAGTGACCGGCTCCTGGAGCAGG
NM_138433.3       CTGCGGGGCGTGGGCGCCGCCGTGATGCGCTACAACACAGTGACCGGCTCCTGGAGCAGG
                  ************************************************************

HsCD00512521      GCTGCCTCCCTGCCCCTGCCCGCCCCCGCCCCACTGCACTGCACCACCCTGGGCAACACC
NM_138433.3       GCTGCCTCCCTGCCCCTGCCCGCCCCCGCCCCACTGCACTGCACCACCCTGGGCAACACC
                  ************************************************************

HsCD00512521      ATTTACTGCCTCAACCCCCAGGTCACTGCCACCTTCACGGTCTCTGGGGGGACTGCCCAG
NM_138433.3       ATTTACTGCCTCAACCCCCAGGTCACTGCCACCTTCACGGTCTCTGGGGGGACTGCCCAG
                  ************************************************************

HsCD00512521      TTCCAGGCCAAGGAGCTGCAGCCCTTCCCCTTGGGGAGCACCGGGGTCCTCAGTCCATTC
NM_138433.3       TTCCAGGCCAAGGAGCTGCAGCCCTTCCCCTTGGGGAGCACCGGGGTCCTCAGTCCATTC
                  ************************************************************

HsCD00512521      ATCCTGACTCTGCCCCCTGAGGACCGGCTGCAGACCTCACTC---
NM_138433.3       ATCCTGACTCTGCCCCCTGAGGACCGGCTGCAGACCTCACTCTGA
                  ******************************************   

Protein

SMART domain : Kelch :
HPRD : 14453

Clone

Cloning Information : 604418
Original Clone ID : ccsbBroadEn_13026

Organism


TAX_ID : 9606
Species Specific ID: 113730

Genome

Chromosome : 22
Map Location : 22q13.33
Ensembl : ENSG00000130487

Reagents

Labome : KLHDC7B-antibody