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CAPN2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN2
Gene Name:  calpain 2, (m/II) large subunit
Sequence             
Original Clone ID: ccsbBroadEn_05933
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC021303.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00512582

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2101nts         Open reading frame : 1 to 2101
Reference Sequence Alignment

ATGGCGGGCATCGCGGCCAAGCTGGCGAAGGACCGGGAGGCGGCCGAGGGGCTGGGCTCC
CACGAGAGGGCCATCAAGTACCTCAACCAGGACTACGAGGCGCTGCGGAACGAGTGCCTG
GAGGCCGGGACGCTCTTCCAGGACCCGTCCTTCCCGGCCATCCCCTCGGCCCTGGGCTTC
AAGGAGTTGGGGCCCTACTCCAGCAAAACCCGGGGCATCGAGTGGAAGCGCCCCACGGAG
ATCTGCGCTGACCCCCAGTTTATCATTGGAGGAGCCACCCGCACAGACATCTGCCAAGGA
GCCCTAGGTGACTGCTGGCTGCTGGCAGCCATTGCCTCCCTCACCTTGAATGAAGAAATC
CTGGCTCGAGTCGTCCCCCTAAACCAGAGCTTCCAGGAAAACTATGCAGGGATCTTTCAC
TTCCAGTTCTGGCAATACGGCGAGTGGGTGGAGGTGGTGGTGGATGACAGGCTGCCCACC
AAGGACGGGGAGCTGCTCTTTGTGCATTCAGCCGAAGGGAGCGAGTTCTGGAGCGCCCTG
CTGGAGAAGGCATACGCCAAGATCAACGGATGCTATGAAGCACTATCAGGGGGTGCCACC
ACTGAGGGCTTCGAAGACTTCACCGGAGGCATTGCTGAGTGGTATGAGTTGAAGAAGCCC
CCTCCCAACCTGTTCAAGATCATCCAGAAAGCTCTGCAAAAAGGCTCTCTCCTTGGCTGC
TCCATCGACATCACCAGCGCCGCGGACTCGGAGGCCATCACGTTTCAGAAGCTGGTGAAG
GGGCACGCGTACTCGGTCACCGGAGCCGAGGAGGTTGAAAGTAACGGAAGCCTACAGAAA
CTGATCCGCATCCGAAATCCCTGGGGAGAAGTGGAGTGGACAGGGCGGTGGAATGACAAC
TGCCCAAGCTGGAACACTATAGACCCAGAGGAGAGGGAAAGGCTGACCAGACGGCATGAA
GATGGAGAATTCTGGATGTCTTTCAGTGACTTCCTGAGGCACTATTCCCGCCTGGAGATC
TGTAACCTGACCCCAGACACTCTCACCAGCGATACCTACAAGAAGTGGAAACTCACCAAA
ATGGATGGGAACTGGAGGCGGGGCTCCACCGCGGGAGGTTGCAGGAACTACCCGAACACA
TTCTGGATGAACCCTCAGTACCTGATCAAGCTGGAGGAGGAGGATGAGGACGAGGAGGAT
GGGGAGAGCGGCTGCACCTTCCTGGTGGGGCTCATTCAGAAGCACCGACGGCGGCAGAGG
AAGATGGGCGAGGACATGCACACCATCGGCTTTGGCATCTATGAGGTTCCAGAGGAGTTA
AGTGGGCAGACCAACATCCACCTCAGCAAAAACTTCTTCCTGACGAATCGCGCCAGGGAG
CGCTCAGACACCTTCATCAACCTCCGGGAGGTGCTCAACCGCTTCAAGCTGCCGCCAGGA
GAGTACATTCTCGTGCCTTCCACCTTCGAACCCAACAAGGATGGGGATTTCTGCATCCGG
GTCTTTTCTGAAAAGAAAGCTGACTACCAAGCTGTCGATGATGAAATCGAGGCCAATCTT
GAAGAGTTCGACATCAGCGAGGATGACATTGATGATGGATTCAGGAGACTGTTTGCCCAG
TTGGCAGGAGAGGATGCGGAGATCTCTGCCTTTGAGCTGCAGACCATCCTGAGAAGGGTT
CTAGCAAAGCGCCAAGATATCAAGTCAGATGGCTTCAGCATCGAGACATGCAAAATTATG
GTTGACATGCTAGATTCGGACGGGAGTGGCAAGCTGGGGCTGAAGGAGTTCTACATTCTC
TGGACGAAGATTCAAAAATACCAAAAAATTTACCGAGAAATCGACGTTGACAGGTCTGGT
ACCATGAATTCCTATGAAATGCGGAAGGCATTAGAAGAAGCAGGTTTCAAGATGCCCTGT
CAACTCCACCAAGTCATCGTTGCTCGGTTTGCAGATGACCAGCTCATCATCGATTTTGAT
AATTTTGTTCGGTGTTTGGTTCGGCTGGAAACGCTATTCAAGATATTTAAGCAGCTGGAT
CCCGAGAATACTGGAACAATAGAGCTCGACCTTATCTCTTGGCTCTGTTTCTCAGTACTT


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN2
Symbol Nomenclature : CAPN2
SYNONYM : CANP2; CANPL2; CANPml; mCANP
Designation : CANP 2|M-calpain|calcium-activated neutral proteinase 2|calpain 2, large [catalytic] subunit|calpain 2, large subunit|calpain M-type|calpain large polypeptide L2|calpain, large polypeptide L2|calpain-2 catalytic subunit|calpain-2 large subunit|millimolar-calpain
Full Nomenclature : calpain 2, (m/II) large subunit
GENEID : 824
GenBank Accession : EU176804
HGNC : 1479
MIM : 114230
Vega : OTTHUMG00000037376
Target GenBank: EU176804

 

Reference Sequence Alignment

  

HsCD00512582      ATGGCGGGCATCGCGGCCAAGCTGGCGAAGGACCGGGAGGCGGCCGAGGGGCTGGGCTCC
NM_001748.4       ATGGCGGGCATCGCGGCCAAGCTGGCGAAGGACCGGGAGGCGGCCGAGGGGCTGGGCTCC
                  ************************************************************

HsCD00512582      CACGAGAGGGCCATCAAGTACCTCAACCAGGACTACGAGGCGCTGCGGAACGAGTGCCTG
NM_001748.4       CACGAGAGGGCCATCAAGTACCTCAACCAGGACTACGAGGCGCTGCGGAACGAGTGCCTG
                  ************************************************************

HsCD00512582      GAGGCCGGGACGCTCTTCCAGGACCCGTCCTTCCCGGCCATCCCCTCGGCCCTGGGCTTC
NM_001748.4       GAGGCCGGGACGCTCTTCCAGGACCCGTCCTTCCCGGCCATCCCCTCGGCCCTGGGCTTC
                  ************************************************************

HsCD00512582      AAGGAGTTGGGGCCCTACTCCAGCAAAACCCGGGGCATCGAGTGGAAGCGCCCCACGGAG
NM_001748.4       AAGGAGTTGGGGCCCTACTCCAGCAAAACCCGGGGCATCGAGTGGAAGCGCCCCACGGAG
                  ************************************************************

HsCD00512582      ATCTGCGCTGACCCCCAGTTTATCATTGGAGGAGCCACCCGCACAGACATCTGCCAAGGA
NM_001748.4       ATCTGCGCTGACCCCCAGTTTATCATTGGAGGAGCCACCCGCACAGACATCTGCCAAGGA
                  ************************************************************

HsCD00512582      GCCCTAGGTGACTGCTGGCTGCTGGCAGCCATTGCCTCCCTCACCTTGAATGAAGAAATC
NM_001748.4       GCCCTGGGTGACTGCTGGCTGCTGGCAGCCATTGCCTCCCTCACCTTGAATGAAGAAATC
                  *****.******************************************************

HsCD00512582      CTGGCTCGAGTCGTCCCCCTAAACCAGAGCTTCCAGGAAAACTATGCAGGGATCTTTCAC
NM_001748.4       CTGGCTCGAGTCGTCCCCCTAAACCAGAGCTTCCAGGAAAACTATGCAGGGATCTTTCAC
                  ************************************************************

HsCD00512582      TTCCAGTTCTGGCAATACGGCGAGTGGGTGGAGGTGGTGGTGGATGACAGGCTGCCCACC
NM_001748.4       TTCCAGTTCTGGCAATACGGCGAGTGGGTGGAGGTGGTGGTGGATGACAGGCTGCCCACC
                  ************************************************************

HsCD00512582      AAGGACGGGGAGCTGCTCTTTGTGCATTCAGCCGAAGGGAGCGAGTTCTGGAGCGCCCTG
NM_001748.4       AAGGACGGGGAGCTGCTCTTTGTGCATTCAGCCGAAGGGAGCGAGTTCTGGAGCGCCCTG
                  ************************************************************

HsCD00512582      CTGGAGAAGGCATACGCCAAGATCAACGGATGCTATGAAGCACTATCAGGGGGTGCCACC
NM_001748.4       CTGGAGAAGGCATACGCCAAGATCAACGGATGCTATGAAGCGCTATCAGGGGGTGCCACC
                  *****************************************.******************

HsCD00512582      ACTGAGGGCTTCGAAGACTTCACCGGAGGCATTGCTGAGTGGTATGAGTTGAAGAAGCCC
NM_001748.4       ACTGAGGGCTTCGAAGACTTCACCGGAGGCATTGCTGAGTGGTATGAGTTGAAGAAGCCC
                  ************************************************************

HsCD00512582      CCTCCCAACCTGTTCAAGATCATCCAGAAAGCTCTGCAAAAAGGCTCTCTCCTTGGCTGC
NM_001748.4       CCTCCCAACCTGTTCAAGATCATCCAGAAAGCTCTGCAAAAAGGCTCTCTCCTTGGCTGC
                  ************************************************************

HsCD00512582      TCCATCGACATCACCAGCGCCGCGGACTCGGAGGCCATCACGTTTCAGAAGCTGGTGAAG
NM_001748.4       TCCATCGACATCACCAGCGCCGCGGACTCGGAGGCCATCACGTTTCAGAAGCTGGTGAAG
                  ************************************************************

HsCD00512582      GGGCACGCGTACTCGGTCACCGGAGCCGAGGAGGTTGAAAGTAACGGAAGCCTACAGAAA
NM_001748.4       GGGCACGCGTACTCGGTCACCGGAGCCGAGGAGGTTGAAAGTAACGGAAGCCTACAGAAA
                  ************************************************************

HsCD00512582      CTGATCCGCATCCGAAATCCCTGGGGAGAAGTGGAGTGGACAGGGCGGTGGAATGACAAC
NM_001748.4       CTGATCCGCATCCGAAATCCCTGGGGAGAAGTGGAGTGGACAGGGCGGTGGAATGACAAC
                  ************************************************************

HsCD00512582      TGCCCAAGCTGGAACACTATAGACCCAGAGGAGAGGGAAAGGCTGACCAGACGGCATGAA
NM_001748.4       TGCCCAAGCTGGAACACTATAGACCCAGAGGAGAGGGAAAGGCTGACCAGACGGCATGAA
                  ************************************************************

HsCD00512582      GATGGAGAATTCTGGATGTCTTTCAGTGACTTCCTGAGGCACTATTCCCGCCTGGAGATC
NM_001748.4       GATGGAGAATTCTGGATGTCTTTCAGTGACTTCCTGAGGCACTATTCCCGCCTGGAGATC
                  ************************************************************

HsCD00512582      TGTAACCTGACCCCAGACACTCTCACCAGCGATACCTACAAGAAGTGGAAACTCACCAAA
NM_001748.4       TGTAACCTGACCCCAGACACTCTCACCAGCGATACCTACAAGAAGTGGAAACTCACCAAA
                  ************************************************************

HsCD00512582      ATGGATGGGAACTGGAGGCGGGGCTCCACCGCGGGAGGTTGCAGGAACTACCCGAACACA
NM_001748.4       ATGGATGGGAACTGGAGGCGGGGCTCCACCGCGGGAGGTTGCAGGAACTACCCGAACACA
                  ************************************************************

HsCD00512582      TTCTGGATGAACCCTCAGTACCTGATCAAGCTGGAGGAGGAGGATGAGGACGAGGAGGAT
NM_001748.4       TTCTGGATGAACCCTCAGTACCTGATCAAGCTGGAGGAGGAGGATGAGGACGAGGAGGAT
                  ************************************************************

HsCD00512582      GGGGAGAGCGGCTGCACCTTCCTGGTGGGGCTCATTCAGAAGCACCGACGGCGGCAGAGG
NM_001748.4       GGGGAGAGCGGCTGCACCTTCCTGGTGGGGCTCATTCAGAAGCACCGACGGCGGCAGAGG
                  ************************************************************

HsCD00512582      AAGATGGGCGAGGACATGCACACCATCGGCTTTGGCATCTATGAGGTTCCAGAGGAGTTA
NM_001748.4       AAGATGGGCGAGGACATGCACACCATCGGCTTTGGCATCTATGAGGTTCCAGAGGAGTTA
                  ************************************************************

HsCD00512582      AGTGGGCAGACCAACATCCACCTCAGCAAAAACTTCTTCCTGACGAATCGCGCCAGGGAG
NM_001748.4       AGTGGGCAGACCAACATCCACCTCAGCAAAAACTTCTTCCTGACGAATCGCGCCAGGGAG
                  ************************************************************

HsCD00512582      CGCTCAGACACCTTCATCAACCTCCGGGAGGTGCTCAACCGCTTCAAGCTGCCGCCAGGA
NM_001748.4       CGCTCAGACACCTTCATCAACCTCCGGGAGGTGCTCAACCGCTTCAAGCTGCCGCCAGGA
                  ************************************************************

HsCD00512582      GAGTACATTCTCGTGCCTTCCACCTTCGAACCCAACAAGGATGGGGATTTCTGCATCCGG
NM_001748.4       GAGTACATTCTCGTGCCTTCCACCTTCGAACCCAACAAGGATGGGGATTTCTGCATCCGG
                  ************************************************************

HsCD00512582      GTCTTTTCTGAAAAGAAAGCTGACTACCAAGCTGTCGATGATGAAATCGAGGCCAATCTT
NM_001748.4       GTCTTTTCTGAAAAGAAAGCTGACTACCAAGCTGTCGATGATGAAATCGAGGCCAATCTT
                  ************************************************************

HsCD00512582      GAAGAGTTCGACATCAGCGAGGATGACATTGATGATGGATTCAGGAGACTGTTTGCCCAG
NM_001748.4       GAAGAGTTCGACATCAGCGAGGATGACATTGATGATGGATTCAGGAGACTGTTTGCCCAG
                  ************************************************************

HsCD00512582      TTGGCAGGAGAGGATGCGGAGATCTCTGCCTTTGAGCTGCAGACCATCCTGAGAAGGGTT
NM_001748.4       TTGGCAGGAGAGGATGCGGAGATCTCTGCCTTTGAGCTGCAGACCATCCTGAGAAGGGTT
                  ************************************************************

HsCD00512582      CTAGCAAAGCGCCAAGATATCAAGTCAGATGGCTTCAGCATCGAGACATGCAAAATTATG
NM_001748.4       CTAGCAAAGCGCCAAGATATCAAGTCAGATGGCTTCAGCATCGAGACATGCAAAATTATG
                  ************************************************************

HsCD00512582      GTTGACATGCTAGATTCGGACGGGAGTGGCAAGCTGGGGCTGAAGGAGTTCTACATTCTC
NM_001748.4       GTTGACATGCTAGATTCGGACGGGAGTGGCAAGCTGGGGCTGAAGGAGTTCTACATTCTC
                  ************************************************************

HsCD00512582      TGGACGAAGATTCAAAAATACCAAAAAATTTACCGAGAAATCGACGTTGACAGGTCTGGT
NM_001748.4       TGGACGAAGATTCAAAAATACCAAAAAATTTACCGAGAAATCGACGTTGACAGGTCTGGT
                  ************************************************************

HsCD00512582      ACCATGAATTCCTATGAAATGCGGAAGGCATTAGAAGAAGCAGGTTTCAAGATGCCCTGT
NM_001748.4       ACCATGAATTCCTATGAAATGCGGAAGGCATTAGAAGAAGCAGGTTTCAAGATGCCCTGT
                  ************************************************************

HsCD00512582      CAACTCCACCAAGTCATCGTTGCTCGGTTTGCAGATGACCAGCTCATCATCGATTTTGAT
NM_001748.4       CAACTCCACCAAGTCATCGTTGCTCGGTTTGCAGATGACCAGCTCATCATCGATTTTGAT
                  ************************************************************

HsCD00512582      AATTTTGTTCGGTGTTTGGTTCGGCTGGAAACGCTATTCAAGATATTTAAGCAGCTGGAT
NM_001748.4       AATTTTGTTCGGTGTTTGGTTCGGCTGGAAACGCTATTCAAGATATTTAAGCAGCTGGAT
                  ************************************************************

HsCD00512582      CCCGAGAATACTGGAACAATAGAGCTCGACCTTATCTCTTGGCTCTGTTTCTCAGTACTT
NM_001748.4       CCCGAGAATACTGGAACAATAGAGCTCGACCTTATCTCTTGGCTCTGTTTCTCAGTACTT
                  ************************************************************

HsCD00512582      ---
NM_001748.4       TGA
                     

Pathways

NCI : ErbB1 downstream signaling
NCI : Signaling events mediated by focal adhesion kinase
Panther : Huntington disease
WikiPathway : Alzheimers Disease
WikiPathway : Integrin-mediated cell adhesion

Protein

SMART domain : CysPc : Calpain-like thiol protease family.
SMART domain : calpain_III :
HPRD : 00254

Clone

Cloning Information : 607254
Original Clone ID : ccsbBroadEn_05933

Organism


TAX_ID : 9606
Species Specific ID: 824

Genome

Chromosome : 1
Map Location : 1q41-q42
Ensembl : ENSG00000162909

Reagents

Labome : CAPN2-antibody