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CCT4 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CCT4
Gene Name:  chaperonin containing TCP1, subunit 4 (delta)
Sequence             
Original Clone ID: ccsbBroadEn_02474
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC106934.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00513287

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1618nts         Open reading frame : 1 to 1618
Reference Sequence Alignment

ATGCCCGAGAATGTGGCACCCCGGAGCGGGGCGACTGCCGGGGCTGCCGGCGGCCGCGGG
AAAGGCGCCTATCAGGACCGCGACAAGCCAGCCCAGATCCGCTTCAGCAACATTTCCGCC
GCCAAAGCGGTTGCTGATGCTATTAGAACAAGCCTTGGACCAAAAGGAATGGATAAAATG
ATTCAAGATGGAAAAGGTGATGTAACCATTACAAATGATGGTGCTACCATTCTGAAACAA
ATGCAAGTATTACATCCAGCAGCCAGAATGCTGGTGGAGCTGTCTAAGGCTCAAGATATA
GAAGCAGGAGATGGCACCACATCAGTAGTCATCATTGCTGGCTCCCTCTTAGATTCTTGT
ACCAAGCTTCTTCAGAAAGGGATTCATCCAACCATCATTTCTGAGTCATTCCAGAAGGCC
CTGGAAAAGGGCATTGAAATCTTGACTGACATGTCTCGACCTGTGGAACTGAGTGACAGA
GAAACTTTGTTAAATAGTGCAACCACTTCACTGAACTCAAAGGTGGTTTCTCAGTATTCA
AGTCTGCTTTCTCCAATGAGTGTAAATGCAGTGATGAAAGTGATTGACCCAGCCACAGCC
ACCAGTGTAGATCTTAGAGATATTAAAATAGTTAAGAAGCTTGGTGGGACAATTGATGAC
TGTGAGTTGGTGGAAGGGCTGGTTCTCACCCAAAAAGTGTCAAATTCTGGCATAACCAGA
GTTGAAAAGGCCAAGATTGGGCTTATTCAGTTTTGCTTATCTGCTCCCAAAACAGACATG
GATAATCAAATAGTGGTTTCTGACTATGCCCAGATGGACCGAGTGCTGCGAGAAGAGAGA
GCCTATATTTTAAATTTAGTGAAGCAAATTAAAAAAACAGGATGTAATGTCCTTCTCATA
CAGAAATCTATTCTAAGAGATGCTCTTAGTGATCTTGCATTACACTTTCTGAATAAAATG
AAGATCATGGTGATTAAGGATATTGAAAGAGAAGACATTGAATTCATTTGTAAGACAATT
GGAACCAAGCCAGTTGCTCATATTGACCAATTTACTGCTGACATGCTGGGTTCTGCTGAG
TTAGCTGAGGAGGTCAATTTAAATGGTTCTGGCAAACTGCTCAAGATTACAGGCTGTGCC
AGCCCTGGAAAAACAGTTACAATTGTTGTTCGTGGTTCTAACAAACTGGTGATTGAAGAA
GCTGAGCGCTCCATTCATGATGCCCTATGTGTTATTCGTTGTTTAGTGAAGAAGAGGGCT
CTTATTGCAGGAGGTGGTGCTCCAGAAATAGAGTTGGCCCTACGATTAACTGAATATTCA
CGAACACTGAGTGGTATGGAATCCTACTGCGTTCGTGCTTTTGCAGATGCTATGGAGGTC
ATTCCATCTACACTAGCTGAAAATGCCGGCCTGAATCCCATTTCTACAGTAACAGAACTA
AGAAACCGGCATGCCCAGGGAGAAAAAACTGCAGGCATTAATGTCCGAAAGGGTGGTATT
TCCAACATTTTGGAGGAACTGGTTGTCCAGCCTCTGTTGGTATCAGTCAGTGCTCTGACT
CTTGCAACTGAAACTGTTCGGAGCATTCTGAAAATAGATGATGTGGTAAACACTCGA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CCT4
Symbol Nomenclature : CCT4
SYNONYM : CCT-DELTA; Cctd; SRB
Designation : T-complex protein 1 subunit delta|TCP-1-delta|chaperonin containing t-complex polypeptide 1, delta subunit|stimulator of TAR RNA-binding
Full Nomenclature : chaperonin containing TCP1, subunit 4 (delta)
GENEID : 10575
GenBank Accession : EU446763
HGNC : 1617
MIM : 605142
Vega : OTTHUMG00000152166
Target GenBank: EU446763

 

Reference Sequence Alignment

  

HsCD00513287        ATGCCCGAGAATGTGGCACCCCGGAGCGGGGCGACTGCCGGGGCTGCCGGCGGCCGCGGG
NM_006430.3         ATGCCCGAGAATGTGGCACCCCGGAGCGGGGCGACTGCCGGGGCTGCCGGCGGCCGCGGG
NM_001256721.1      ATGCCCGAGAATGTGGCACCCCGGAGCGGGGCGACTGCCGGGGCTGCCGGCGGCCGCGGG
                    ************************************************************

HsCD00513287        AAAGGCGCCTATCAGGACCGCGACAAGCCAGCCCAGATCCGCTTCAGCAACATTTCCGCC
NM_006430.3         AAAGGCGCCTATCAGGACCGCGACAAGCCAGCCCAGATCCGCTTCAGCAACATTTCCGCC
NM_001256721.1      AAAGGCGCCTATCAGGACCGCGACAAGCCAGCCCAGATCCGCTTCAGCAACATTTCCGCC
                    ************************************************************

HsCD00513287        GCCAAAGCGGTTGCTGATGCTATTAGAACAAGCCTTGGACCAAAAGGAATGGATAAAATG
NM_006430.3         GCCAAAGCGGTTGCTGATGCTATTAGAACAAGCCTTGGACCAAAAGGAATGGATAAAATG
NM_001256721.1      GCCAAAGCGGTTGCTGATGCTATTAGAACAAGCCTTGGACCAAAAGGAATG---------
                    ***************************************************         

HsCD00513287        ATTCAAGATGGAAAAGGTGATGTAACCATTACAAATGATGGTGCTACCATTCTGAAACAA
NM_006430.3         ATTCAAGATGGAAAAGGTGATGTAACCATTACAAATGATGGTGCTACCATTCTGAAACAA
NM_001256721.1      ------------------------------------------------------------
                                                                                

HsCD00513287        ATGCAAGTATTACATCCAGCAGCCAGAATGCTGGTGGAGCTGTCTAAGGCTCAAGATATA
NM_006430.3         ATGCAAGTATTACATCCAGCAGCCAGAATGCTGGTGGAGCTGTCTAAGGCTCAAGATATA
NM_001256721.1      ---------------------GATAAAATGCTGGTGGAGCTGTCTAAGGCTCAAGATATA
                                         *. *.**********************************

HsCD00513287        GAAGCAGGAGATGGCACCACATCAGTAGTCATCATTGCTGGCTCCCTCTTAGATTCTTGT
NM_006430.3         GAAGCAGGAGATGGCACCACATCAGTAGTCATCATTGCTGGCTCCCTCTTAGATTCTTGT
NM_001256721.1      GAAGCAGGAGATGGCACCACATCAGTAGTCATCATTGCTGGCTCCCTCTTAGATTCTTGT
                    ************************************************************

HsCD00513287        ACCAAGCTTCTTCAGAAAGGGATTCATCCAACCATCATTTCTGAGTCATTCCAGAAGGCC
NM_006430.3         ACCAAGCTTCTTCAGAAAGGGATTCATCCAACCATCATTTCTGAGTCATTCCAGAAGGCC
NM_001256721.1      ACCAAGCTTCTTCAGAAAGGGATTCATCCAACCATCATTTCTGAGTCATTCCAGAAGGCC
                    ************************************************************

HsCD00513287        CTGGAAAAGGGCATTGAAATCTTGACTGACATGTCTCGACCTGTGGAACTGAGTGACAGA
NM_006430.3         CTGGAAAAGGGCATTGAAATCTTGACTGACATGTCTCGACCTGTGGAACTGAGTGACAGA
NM_001256721.1      CTGGAAAAGGGCATTGAAATCTTGACTGACATGTCTCGACCTGTGGAACTGAGTGACAGA
                    ************************************************************

HsCD00513287        GAAACTTTGTTAAATAGTGCAACCACTTCACTGAACTCAAAGGTGGTTTCTCAGTATTCA
NM_006430.3         GAAACTTTGTTAAATAGTGCAACCACTTCACTGAACTCAAAGGTGGTTTCTCAGTATTCA
NM_001256721.1      GAAACTTTGTTAAATAGTGCAACCACTTCACTGAACTCAAAGGTGGTTTCTCAGTATTCA
                    ************************************************************

HsCD00513287        AGTCTGCTTTCTCCAATGAGTGTAAATGCAGTGATGAAAGTGATTGACCCAGCCACAGCC
NM_006430.3         AGTCTGCTTTCTCCAATGAGTGTAAATGCAGTGATGAAAGTGATTGACCCAGCCACAGCC
NM_001256721.1      AGTCTGCTTTCTCCAATGAGTGTAAATGCAGTGATGAAAGTGATTGACCCAGCCACAGCC
                    ************************************************************

HsCD00513287        ACCAGTGTAGATCTTAGAGATATTAAAATAGTTAAGAAGCTTGGTGGGACAATTGATGAC
NM_006430.3         ACCAGTGTAGATCTTAGAGATATTAAAATAGTTAAGAAGCTTGGTGGGACAATTGATGAC
NM_001256721.1      ACCAGTGTAGATCTTAGAGATATTAAAATAGTTAAGAAGCTTGGTGGGACAATTGATGAC
                    ************************************************************

HsCD00513287        TGTGAGTTGGTGGAAGGGCTGGTTCTCACCCAAAAAGTGTCAAATTCTGGCATAACCAGA
NM_006430.3         TGTGAGTTGGTGGAAGGGCTGGTTCTCACCCAAAAAGTGTCAAATTCTGGCATAACCAGA
NM_001256721.1      TGTGAGTTGGTGGAAGGGCTGGTTCTCACCCAAAAAGTGTCAAATTCTGGCATAACCAGA
                    ************************************************************

HsCD00513287        GTTGAAAAGGCCAAGATTGGGCTTATTCAGTTTTGCTTATCTGCTCCCAAAACAGACATG
NM_006430.3         GTTGAAAAGGCCAAGATTGGGCTTATTCAGTTTTGCTTATCTGCTCCCAAAACAGACATG
NM_001256721.1      GTTGAAAAGGCCAAGATTGGGCTTATTCAGTTTTGCTTATCTGCTCCCAAAACAGACATG
                    ************************************************************

HsCD00513287        GATAATCAAATAGTGGTTTCTGACTATGCCCAGATGGACCGAGTGCTGCGAGAAGAGAGA
NM_006430.3         GATAATCAAATAGTGGTTTCTGACTATGCCCAGATGGACCGAGTGCTGCGAGAAGAGAGA
NM_001256721.1      GATAATCAAATAGTGGTTTCTGACTATGCCCAGATGGACCGAGTGCTGCGAGAAGAGAGA
                    ************************************************************

HsCD00513287        GCCTATATTTTAAATTTAGTGAAGCAAATTAAAAAAACAGGATGTAATGTCCTTCTCATA
NM_006430.3         GCCTATATTTTAAATTTAGTGAAGCAAATTAAAAAAACAGGATGTAATGTCCTTCTCATA
NM_001256721.1      GCCTATATTTTAAATTTAGTGAAGCAAATTAAAAAAACAGGATGTAATGTCCTTCTCATA
                    ************************************************************

HsCD00513287        CAGAAATCTATTCTAAGAGATGCTCTTAGTGATCTTGCATTACACTTTCTGAATAAAATG
NM_006430.3         CAGAAATCTATTCTAAGAGATGCTCTTAGTGATCTTGCATTACACTTTCTGAATAAAATG
NM_001256721.1      CAGAAATCTATTCTAAGAGATGCTCTTAGTGATCTTGCATTACACTTTCTGAATAAAATG
                    ************************************************************

HsCD00513287        AAGATCATGGTGATTAAGGATATTGAAAGAGAAGACATTGAATTCATTTGTAAGACAATT
NM_006430.3         AAGATCATGGTGATTAAGGATATTGAAAGAGAAGACATTGAATTCATTTGTAAGACAATT
NM_001256721.1      AAGATCATGGTGATTAAGGATATTGAAAGAGAAGACATTGAATTCATTTGTAAGACAATT
                    ************************************************************

HsCD00513287        GGAACCAAGCCAGTTGCTCATATTGACCAATTTACTGCTGACATGCTGGGTTCTGCTGAG
NM_006430.3         GGAACCAAGCCAGTTGCTCATATTGACCAATTTACTGCTGACATGCTGGGTTCTGCTGAG
NM_001256721.1      GGAACCAAGCCAGTTGCTCATATTGACCAATTTACTGCTGACATGCTGGGTTCTGCTGAG
                    ************************************************************

HsCD00513287        TTAGCTGAGGAGGTCAATTTAAATGGTTCTGGCAAACTGCTCAAGATTACAGGCTGTGCC
NM_006430.3         TTAGCTGAGGAGGTCAATTTAAATGGTTCTGGCAAACTGCTCAAGATTACAGGCTGTGCC
NM_001256721.1      TTAGCTGAGGAGGTCAATTTAAATGGTTCTGGCAAACTGCTCAAGATTACAGGCTGTGCC
                    ************************************************************

HsCD00513287        AGCCCTGGAAAAACAGTTACAATTGTTGTTCGTGGTTCTAACAAACTGGTGATTGAAGAA
NM_006430.3         AGCCCTGGAAAAACAGTTACAATTGTTGTTCGTGGTTCTAACAAACTGGTGATTGAAGAA
NM_001256721.1      AGCCCTGGAAAAACAGTTACAATTGTTGTTCGTGGTTCTAACAAACTGGTGATTGAAGAA
                    ************************************************************

HsCD00513287        GCTGAGCGCTCCATTCATGATGCCCTATGTGTTATTCGTTGTTTAGTGAAGAAGAGGGCT
NM_006430.3         GCTGAGCGCTCCATTCATGATGCCCTATGTGTTATTCGTTGTTTAGTGAAGAAGAGGGCT
NM_001256721.1      GCTGAGCGCTCCATTCATGATGCCCTATGTGTTATTCGTTGTTTAGTGAAGAAGAGGGCT
                    ************************************************************

HsCD00513287        CTTATTGCAGGAGGTGGTGCTCCAGAAATAGAGTTGGCCCTACGATTAACTGAATATTCA
NM_006430.3         CTTATTGCAGGAGGTGGTGCTCCAGAAATAGAGTTGGCCCTACGATTAACTGAATATTCA
NM_001256721.1      CTTATTGCAGGAGGTGGTGCTCCAGAAATAGAGTTGGCCCTACGATTAACTGAATATTCA
                    ************************************************************

HsCD00513287        CGAACACTGAGTGGTATGGAATCCTACTGCGTTCGTGCTTTTGCAGATGCTATGGAGGTC
NM_006430.3         CGAACACTGAGTGGTATGGAATCCTACTGCGTTCGTGCTTTTGCAGATGCTATGGAGGTC
NM_001256721.1      CGAACACTGAGTGGTATGGAATCCTACTGCGTTCGTGCTTTTGCAGATGCTATGGAGGTC
                    ************************************************************

HsCD00513287        ATTCCATCTACACTAGCTGAAAATGCCGGCCTGAATCCCATTTCTACAGTAACAGAACTA
NM_006430.3         ATTCCATCTACACTAGCTGAAAATGCCGGCCTGAATCCCATTTCTACAGTAACAGAACTA
NM_001256721.1      ATTCCATCTACACTAGCTGAAAATGCCGGCCTGAATCCCATTTCTACAGTAACAGAACTA
                    ************************************************************

HsCD00513287        AGAAACCGGCATGCCCAGGGAGAAAAAACTGCAGGCATTAATGTCCGAAAGGGTGGTATT
NM_006430.3         AGAAACCGGCATGCCCAGGGAGAAAAAACTGCAGGCATTAATGTCCGAAAGGGTGGTATT
NM_001256721.1      AGAAACCGGCATGCCCAGGGAGAAAAAACTGCAGGCATTAATGTCCGAAAGGGTGGTATT
                    ************************************************************

HsCD00513287        TCCAACATTTTGGAGGAACTGGTTGTCCAGCCTCTGTTGGTATCAGTCAGTGCTCTGACT
NM_006430.3         TCCAACATTTTGGAGGAACTGGTTGTCCAGCCTCTGTTGGTATCAGTCAGTGCTCTGACT
NM_001256721.1      TCCAACATTTTGGAGGAACTGGTTGTCCAGCCTCTGTTGGTATCAGTCAGTGCTCTGACT
                    ************************************************************

HsCD00513287        CTTGCAACTGAAACTGTTCGGAGCATTCTGAAAATAGATGATGTGGTAAACACTCGA---
NM_006430.3         CTTGCAACTGAAACTGTTCGGAGCATTCTGAAAATAGATGATGTGGTAAACACTCGATAA
NM_001256721.1      CTTGCAACTGAAACTGTTCGGAGCATTCTGAAAATAGATGATGTGGTAAACACTCGATAA
                    *********************************************************   

Pathways

Reactome : Association of TriC/CCT with target proteins during biosynthesis
Reactome : Chaperonin-mediated protein folding
Reactome : Cooperation of Prefoldin and TriC/CCT in actin and tubulin folding
Reactome : Folding of actin by CCT/TriC
Reactome : Formation of tubulin folding intermediates by CCT/TriC
Reactome : Metabolism of proteins
Reactome : Prefoldin mediated transfer of substrate to CCT/TriC
Reactome : Protein folding

Protein

HPRD : 05506

Clone

Cloning Information : 607207
Original Clone ID : ccsbBroadEn_02474

Organism


TAX_ID : 9606
Species Specific ID: 10575

Genome

Chromosome : 2
Map Location : 2p15
Ensembl : ENSG00000115484

Reagents

Labome : CCT4-antibody