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CYP2U1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CYP2U1
Gene Name:  cytochrome P450, family 2, subfamily U, polypeptide 1
Sequence             
Original Clone ID: ccsbBroadEn_09387
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC132767.1
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00513388

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1633nts         Open reading frame : 1 to 1633
Reference Sequence Alignment

ATGTCGTCTCCGGGGCCGTCGCAGCCGCCGGCCGAGGACCCGCCCTGGCCCGCGCGCCTC
CTGCGTGCGCCTCTGGGGCTGCTGCGGCTGGNCCCCAGCGGGGGCGCGCTGCTGCTATGC
GGCCTCGTAGCGCTGCTGGGCTGGAGCTGGCTGCGGAGGCGCCGGGCGCGGGGCATCCCG
CCCGGGCCCACGCCCTGGCCTCTGGTGGGCAACTTCGGTCACGTGCTGCTGCCTCCCTTC
CTCCGGCGGCGGAGCTGGCTGAGCAGCAGGACCAGGGCCGCAGGGATTGATCCCTCGGTC
ATAGGCCCGCAGGTGCTCCTGGCTCACCTAGCCCGCGTGTACGGCAGCATCTTCAGCTTC
TTTATCGGCCACTACCTGGTGGTGGTCCTCAGCGACTTCCACAGCGTGCGCGAGGCGCTG
GTGCAGCAGGCCGAGGTCTTCAGCGACCGCCCGCGGGTGCCGCTCATCTCCATCGTGACC
AAGGAGAAGGGGGTTGTGTTTGCACATTATGGTCCCGTCTGGAGACAACAAAGGAAGTTC
TCTCATTCAACTCTTCGTCATTTTGGGTTGGGAAAACTTAGCTTGGAGCCCAAGATTATT
GAGGAGTTCAAATATGTGAAAGCAGAAATGCAAAAGCACGGAGAAGACCCCTTCTGCCCT
TTCTCCATCATCAGCAATGCCGTCTCTAACATCATTTGCTCCTTGTGCTTTGGCCAGCGC
TTTGATTACACTAATAGTGAGTTCAAGAAAATGCTTGGTTTTATGTCACGAGGCCTAGAA
ATCTGTCTGAACAGTCAAGTCCTCCTGGTCAACATATGCCCTTGGCTTTATTACCTTCCC
TTTGGACCATTTAAGGAATTAAGACAAATTGAAAAGGATATAACCAGTTTCCTTAAAAAA
ATCATCAAAGACCATCAAGAGTCTCTGGATAGAGAGAACCCTCAGGACTTCATAGACATG
TACCTTCTCCACATGGAAGAGGAGAGGAAAAATAATAGTAACAGCAGTTTTGATGAAGAG
TACTTATTTTATATCATTGGGGATCTCTTTATTGCTGGGACTGATACCACAACTAACTCT
TTGCTCTGGTGCCTGCTGTATATGTCGCTGAACCCCGATGTACAAGAAAAGGTTCATGAA
GAAATTGAAAGAGTCATTGGCGCCAACCGAGCTCCTTCCCTCACAGACAAGGCCCAGATG
CCCTACACAGAAGCCACCATCATGGAAGTGCAGAGGCTAACTGTGGTGGTGCCGCTTGCC
ATTCCTCATATGACCTCAGAGAACACAGTGCTCCAAGGGTATACCATTCCTAAAGGCACA
TTGATCTTACCCAACCTGTGGTCAGTACATAGAGACCCAGCCATTTGGGAGAAACCGGAG
GATTTCTACCCTAATCGATTTCTGGATGACCAAGGACAACTAATTAAAAAAGAAACCTTT
ATTCCTTTTGGGATAGGGAAGCGGGTGTGTATGGGAGAACAACTGGCAAAGATGGAATTA
TTCCTAATGTTTGTGAGCCTAATGCAGAGTTTCGCATTTGCTTTACCTGAGGATTCTAAG
AAGCCCCTCCTGACTGGAAGATTTGGTCTAACTTTAGCCCCACATCCATTTAATATAACT
ATTTCAAGGAGA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
1 error; 1 non-synonomous errors;
 Clone mixture?
                                

5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CYP2U1
Symbol Nomenclature : CYP2U1
SYNONYM : P450TEC; SPG49; SPG56
Designation : cytochrome P450 2U1|spastic paraplegia 49
Full Nomenclature : cytochrome P450, family 2, subfamily U, polypeptide 1
GENEID : 113612
GenBank Accession : HQ258621
HGNC : 20582
MIM : 610670
Vega : OTTHUMG00000161084
Target GenBank: HQ258621

 

Reference Sequence Alignment

  

HsCD00513388        ATGTCGTCTCCGGGGCCGTCGCAGCCGCCGGCCGAGGACCCGCCCTGGCCCGCGCGCCTC
NM_183075.2         ATGTCGTCTCCGGGGCCGTCGCAGCCGCCGGCCGAGGACCCGCCCTGGCCCGCGCGCCTC
XM_005262717.1      ATGTCGTCTCCGGGGCCGTCGCAGCCGCCGGCCGAGGACCCGCCCTGGCCCGCGCGCCTC
                    ************************************************************

HsCD00513388        CTGCGTGCGCCTCTGGGGCTGCTGCGGCTGGNCCCCAGCGGGGGCGCGCTGCTGCTATGC
NM_183075.2         CTGCGTGCGCCTCTGGGGCTGCTGCGGCTGGACCCCAGCGGGGGCGCGCTGCTGCTATGC
XM_005262717.1      CTGCGTGCGCCTCTGGGGCTGCTGCGGCTGGACCCCAGCGGGGGCGCGCTGCTGCTATGC
                    ******************************* ****************************

HsCD00513388        GGCCTCGTAGCGCTGCTGGGCTGGAGCTGGCTGCGGAGGCGCCGGGCGCGGGGCATCCCG
NM_183075.2         GGCCTCGTAGCGCTGCTGGGCTGGAGCTGGCTGCGGAGGCGCCGGGCGCGGGGCATCCCG
XM_005262717.1      GGCCTCGTAGCGCTGCTGGGCTGGAGCTGGCTGCGGAGGCGCCGGGCGCGGGGCATCCCG
                    ************************************************************

HsCD00513388        CCCGGGCCCACGCCCTGGCCTCTGGTGGGCAACTTCGGTCACGTGCTGCTGCCTCCCTTC
NM_183075.2         CCCGGGCCCACGCCCTGGCCTCTGGTGGGCAACTTCGGTCACGTGCTGCTGCCTCCCTTC
XM_005262717.1      CCCGGGCCCACGCCCTGGCCTCTGGTGGGCAACTTCGGTCACGTGCTGCTGCCTCCCTTC
                    ************************************************************

HsCD00513388        CTCCGGCGGCGGAGCTGGCTGAGCAGCAGGACCAGGGCCGCAGGGATTGATCCCTCGGTC
NM_183075.2         CTCCGGCGGCGGAGCTGGCTGAGCAGCAGGACCAGGGCCGCAGGGATTGATCCCTCGGTC
XM_005262717.1      CTCCGGCGGCGGAGCTGGCTGAGCAGCAGGACCAGGGCCGCAGGGATTGATCCCTCGGTC
                    ************************************************************

HsCD00513388        ATAGGCCCGCAGGTGCTCCTGGCTCACCTAGCCCGCGTGTACGGCAGCATCTTCAGCTTC
NM_183075.2         ATAGGCCCGCAGGTGCTCCTGGCTCACCTAGCCCGCGTGTACGGCAGCATCTTCAGCTTC
XM_005262717.1      ATAGGCCCGCAGGTGCTCCTGGCTCACCTAGCCCGCGTGTACGGCAGCATCTTCAGCTTC
                    ************************************************************

HsCD00513388        TTTATCGGCCACTACCTGGTGGTGGTCCTCAGCGACTTCCACAGCGTGCGCGAGGCGCTG
NM_183075.2         TTTATCGGCCACTACCTGGTGGTGGTCCTCAGCGACTTCCACAGCGTGCGCGAGGCGCTG
XM_005262717.1      TTTATCGGCCACTACCTGGTGGTGGTCCTCAGCGACTTCCACAGCGTGCGCGAGGCGCTG
                    ************************************************************

HsCD00513388        GTGCAGCAGGCCGAGGTCTTCAGCGACCGCCCGCGGGTGCCGCTCATCTCCATCGTGACC
NM_183075.2         GTGCAGCAGGCCGAGGTCTTCAGCGACCGCCCGCGGGTGCCGCTCATCTCCATCGTGACC
XM_005262717.1      GTGCAGCAGGCCGAGGTCTTCAGCGACCGCCCGCGGGTGCCGCTCATCTCCATCGTGACC
                    ************************************************************

HsCD00513388        AAGGAGAAGG--------------------------------------------------
NM_183075.2         AAGGAGAAGG--------------------------------------------------
XM_005262717.1      AAGGAGAAGGGTGAGCGGGAGGTCGTGGGCTGTGGGTACGCGGATGCCGCGGATGAGTCT
                    **********                                                  

HsCD00513388        ----GGGTTGTGTTTGCACATTATGGTCCCGTCTGGAGACAACAAAGGAAGTTCTCTCAT
NM_183075.2         ----GGGTTGTGTTTGCACATTATGGTCCCGTCTGGAGACAACAAAGGAAGTTCTCTCAT
XM_005262717.1      CCAGGGGTTGTGTTTGCACATTATGGTCCCGTCTGGAGACAACAAAGGAAGTTCTCTCAT
                        ********************************************************

HsCD00513388        TCAACTCTTCGTCATTTTGGGTTGGGAAAACTTAGCTTGGAGCCCAAGATTATTGAGGAG
NM_183075.2         TCAACTCTTCGTCATTTTGGGTTGGGAAAACTTAGCTTGGAGCCCAAGATTATTGAGGAG
XM_005262717.1      TCAACTCTTCGTCATTTTGGGTTGGGAAAACTTAGCTTGGAGCCCAAGATTATTGAGGAG
                    ************************************************************

HsCD00513388        TTCAAATATGTGAAAGCAGAAATGCAAAAGCACGGAGAAGACCCCTTCTGCCCTTTCTCC
NM_183075.2         TTCAAATATGTGAAAGCAGAAATGCAAAAGCACGGAGAAGACCCCTTCTGCCCTTTCTCC
XM_005262717.1      TTCAAATATGTGAAAGCAGAAATGCAAAAGCACGGAGAAGACCCCTTCTGCCCTTTCTCC
                    ************************************************************

HsCD00513388        ATCATCAGCAATGCCGTCTCTAACATCATTTGCTCCTTGTGCTTTGGCCAGCGCTTTGAT
NM_183075.2         ATCATCAGCAATGCCGTCTCTAACATCATTTGCTCCTTGTGCTTTGGCCAGCGCTTTGAT
XM_005262717.1      ATCATCAGCAATGCCGTCTCTAACATCATTTGCTCCTTGTGCTTTGGCCAGCGCTTTGAT
                    ************************************************************

HsCD00513388        TACACTAATAGTGAGTTCAAGAAAATGCTTGGTTTTATGTCACGAGGCCTAGAAATCTGT
NM_183075.2         TACACTAATAGTGAGTTCAAGAAAATGCTTGGTTTTATGTCACGAGGCCTAGAAATCTGT
XM_005262717.1      TACACTAATAGTGAGTTCAAGAAAATGCTTGGTTTTATGTCACGAGGCCTAGAAATCTGT
                    ************************************************************

HsCD00513388        CTGAACAGTCAAGTCCTCCTGGTCAACATATGCCCTTGGCTTTATTACCTTCCCTTTGGA
NM_183075.2         CTGAACAGTCAAGTCCTCCTGGTCAACATATGCCCTTGGCTTTATTACCTTCCCTTTGGA
XM_005262717.1      CTGAACAGTCAAGTCCTCCTGGTCAACATATGCCCTTGGCTTTATTACCTTCCCTTTGGA
                    ************************************************************

HsCD00513388        CCATTTAAGGAATTAAGACAAATTGAAAAGGATATAACCAGTTTCCTTAAAAAAATCATC
NM_183075.2         CCATTTAAGGAATTAAGACAAATTGAAAAGGATATAACCAGTTTCCTTAAAAAAATCATC
XM_005262717.1      CCATTTAAGGAATTAAGACAAATTGAAAAGGATATAACCAGTTTCCTTAAAAAAATCATC
                    ************************************************************

HsCD00513388        AAAGACCATCAAGAGTCTCTGGATAGAGAGAACCCTCAGGACTTCATAGACATGTACCTT
NM_183075.2         AAAGACCATCAAGAGTCTCTGGATAGAGAGAACCCTCAGGACTTCATAGACATGTACCTT
XM_005262717.1      AAAGACCATCAAGAGTCTCTGGATAGAGAGAACCCTCAGGACTTCATAGACATGTACCTT
                    ************************************************************

HsCD00513388        CTCCACATGGAAGAGGAGAGGAAAAATAATAGTAACAGCAGTTTTGATGAAGAGTACTTA
NM_183075.2         CTCCACATGGAAGAGGAGAGGAAAAATAATAGTAACAGCAGTTTTGATGAAGAGTACTTA
XM_005262717.1      CTCCACATGGAAGAGGAGAGGAAAAATAATAGTAACAGCAGTTTTGATGAAGAGTACTTA
                    ************************************************************

HsCD00513388        TTTTATATCATTGGGGATCTCTTTATTGCTGGGACTGATACCACAACTAACTCTTTGCTC
NM_183075.2         TTTTATATCATTGGGGATCTCTTTATTGCTGGGACTGATACCACAACTAACTCTTTGCTC
XM_005262717.1      TTTTATATCATTGGGGATCTCTTTATTGCTGGGACTGATACCACAACTAACTCTTTGCTC
                    ************************************************************

HsCD00513388        TGGTGCCTGCTGTATATGTCGCTGAACCCCGATGTACAAGAAAAGGTTCATGAAGAAATT
NM_183075.2         TGGTGCCTGCTGTATATGTCGCTGAACCCCGATGTACAAGAAAAGGTTCATGAAGAAATT
XM_005262717.1      TGGTGCCTGCTGTATATGTCGCTGAACCCCGATGTACAAGAAAAGGTTCATGAAGAAATT
                    ************************************************************

HsCD00513388        GAAAGAGTCATTGGCGCCAACCGAGCTCCTTCCCTCACAGACAAGGCCCAGATGCCCTAC
NM_183075.2         GAAAGAGTCATTGGCGCCAACCGAGCTCCTTCCCTCACAGACAAGGCCCAGATGCCCTAC
XM_005262717.1      GAAAGAGTCATTGGCGCCAACCGAGCTCCTTCCCTCACAGACAAGGCCCAGATGCCCTAC
                    ************************************************************

HsCD00513388        ACAGAAGCCACCATCATGGAAGTGCAGAGGCTAACTGTGGTGGTGCCGCTTGCCATTCCT
NM_183075.2         ACAGAAGCCACCATCATGGAAGTGCAGAGGCTAACTGTGGTGGTGCCGCTTGCCATTCCT
XM_005262717.1      ACAGAAGCCACCATCATGGAAGTGCAGAGGCTAACTGTGGTGGTGCCGCTTGCCATTCCT
                    ************************************************************

HsCD00513388        CATATGACCTCAGAGAACACAGTGCTCCAAGGGTATACCATTCCTAAAGGCACATTGATC
NM_183075.2         CATATGACCTCAGAGAACACAGTGCTCCAAGGGTATACCATTCCTAAAGGCACATTGATC
XM_005262717.1      CATATGACCTCAGAGAACACAGTGCTCCAAGGGTATACCATTCCTAAAGGCACATTGATC
                    ************************************************************

HsCD00513388        TTACCCAACCTGTGGTCAGTACATAGAGACCCAGCCATTTGGGAGAAACCGGAGGATTTC
NM_183075.2         TTACCCAACCTGTGGTCAGTACATAGAGACCCAGCCATTTGGGAGAAACCGGAGGATTTC
XM_005262717.1      TTACCCAACCTGTGGTCAGTACATAGAGACCCAGCCATTTGGGAGAAACCGGAGGATTTC
                    ************************************************************

HsCD00513388        TACCCTAATCGATTTCTGGATGACCAAGGACAACTAATTAAAAAAGAAACCTTTATTCCT
NM_183075.2         TACCCTAATCGATTTCTGGATGACCAAGGACAACTAATTAAAAAAGAAACCTTTATTCCT
XM_005262717.1      TACCCTAATCGATTTCTGGATGACCAAGGACAACTAATTAAAAAAGAAACCTTTATTCCT
                    ************************************************************

HsCD00513388        TTTGGGATAGGGAAGCGGGTGTGTATGGGAGAACAACTGGCAAAGATGGAATTATTCCTA
NM_183075.2         TTTGGGATAGGGAAGCGGGTGTGTATGGGAGAACAACTGGCAAAGATGGAATTATTCCTA
XM_005262717.1      TTTGGGATAGGGAAGCGGGTGTGTATGGGAGAACAACTGGCAAAGATGGAATTATTCCTA
                    ************************************************************

HsCD00513388        ATGTTTGTGAGCCTAATGCAGAGTTTCGCATTTGCTTTACCTGAGGATTCTAAGAAGCCC
NM_183075.2         ATGTTTGTGAGCCTAATGCAGAGTTTCGCATTTGCTTTACCTGAGGATTCTAAGAAGCCC
XM_005262717.1      ATGTTTGTGAGCCTAATGCAGAGTTTCGCATTTGCTTTACCTGAGGATTCTAAGAAGCCC
                    ************************************************************

HsCD00513388        CTCCTGACTGGAAGATTTGGTCTAACTTTAGCCCCACATCCATTTAATATAACTATTTCA
NM_183075.2         CTCCTGACTGGAAGATTTGGTCTAACTTTAGCCCCACATCCATTTAATATAACTATTTCA
XM_005262717.1      CTCCTGACTGGAAGATTTGGTCTAACTTTAGCCCCACATCCATTTAATATAACTATTTCA
                    ************************************************************

HsCD00513388        AGGAGA---
NM_183075.2         AGGAGATGA
XM_005262717.1      AGGAGATGA
                    ******   

Pathways

Reactome : Arachidonic acid metabolism
Reactome : Biological oxidations
Reactome : Cytochrome P450 - arranged by substrate type
Reactome : Metabolism
Reactome : Metabolism of lipids and lipoproteins
Reactome : Miscellaneous substrates
Reactome : Phase 1 - Functionalization of compounds
Reactome : Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE)
WikiPathway : cytochrome P450
WikiPathway : metapathway biotransformation

Protein

HPRD : 13109

Clone

Cloning Information : 606086
Original Clone ID : ccsbBroadEn_09387

Organism


TAX_ID : 9606
Species Specific ID: 113612

Genome

Chromosome : 4
Map Location : 4q25
Ensembl : ENSG00000155016

Reagents

Labome : CYP2U1-antibody