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KBTBD10 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL41
Gene Name:  kelch-like family member 41
Sequence             
Original Clone ID: ccsbBroadEn_02400
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC006534.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00513535

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1819nts         Open reading frame : 1 to 1819
Reference Sequence Alignment

ATGGATTCCCAGCGGGAACTTGCAGAGGAACTGCGGCTTTACCAATCCACCCTTCTTCAG
GATGGTCTAAAAGATCTCCTGGATGAGAAAAAATTCATCGATTGCACCCTAAAAGCAGGT
GACAAAAGTCTTCCTTGCCACAGATTGATTTTGTCAGCTTGTAGTCCTTACTTCCGTGAG
TACTTTTTATCTGAAATTGATGAGGCGAAAAAAAAGGAGGTAGTGCTAGACAATGTGGAT
CCTGCTATACTTGATTTAATCATCAAATACCTGTACTCTGCCAGTATTGATCTCAATGAC
GGAAATGTGCAAGATATTTTTGCATTGGCCAGCCGCTTTCAGATCCCCTCAGTGTTTACT
GTCTGCGTTTCTTATCTTCAGAAAAGACTTGCTCCTGGTAACTGTCTAGCCATCCTAAGA
TTAGGACTTCTTCTTGACTGCCCGAGACTCGCCATTTCTGCCCGTGAATTTGTGTCTGAT
CGCTTTGTACAGATTTGTAAGGAAGAGGACTTTATGCAACTGTCTCCACAGGAACTGATC
TCAGTCATTTCAAATGACAGCCTAAATGTAGAAAAAGAAGAAGCAGTATTTGAGGCAGTG
ATGAAATGGGTGCGAACAGACAAGGAAAACAGGGTTAAAAACCTTAGTGAAGTGTTTGAT
TGTATCCGTTTTCGCCTTATGACAGAAAAATATTTTAAGGATCATGTTGAGAAAGATGAT
ATAATTAAAAGCAACCCAGACCTCCAGAAAAAAATCAAAGTTCTAAAAGATGCTTTCGCA
GGCAAACTCCCAGAACCTAGCAAAAATGCCGCGAAGACTGGGGCTGGTGAGGTGAATGGT
GATGTTGGTGATGAAGATTTACTTCCTGGTTACCTGAATGACATTCCCAGGCATGGAATG
TTTGTAAAAGACCTCATCCTCTTGGTTAATGACACAGCAGCAGTGGCTTATGACCCCACG
GAAAATGAATGCTACCTTACTGCACTGGCTGAGCAGATTCCCAGAAATCATTCCAGCATT
GTTACCCAGCAAAATCAGATATATGTGGTAGGAGGACTATATGTGGATGAAGAAAATAAG
GATCAACCTCTACAGTCATACTTCTTCCAGCTCGATAGCATAGCATCTGAATGGGTTGGA
CTTCCACCTCTGCCTTCAGCCAGGTGTCTCTTCGGTCTGGGAGAGGTGGATGATAAAATC
TATGTAGTTGCAGGCAAAGACCTTCAAACAGAGGCTTCGCTGGATTCAGTATTATGCTAT
GATCCTGTGGCTGCAAAATGGAACGAAGTAAAAAAACTCCCTATCAAAGTCTATGGCCAT
AATGTGATTTCACATAAAGGGATGATATATTGTCTAGGAGGAAAGACAGATGACAAAAAA
TGTACAAACAGGGTGTTTATCTTCAACCCCAAAAAAGGAGATTGGAAAGATCTGGCTCCA
ATGAAAATTCCTCGTTCCATGTTTGGAGTAGCAGTCCATAAAGGCAAAATTGTGATTGCA
GGAGGTGTCACTGAAGATGGTCTTTCAGCTTCAGTTGAAGCTTTTGACCTTACAACAAAT
AAATGGGATGTAATGACCGAATTTCCCCAAGAAAGAAGCTCCATCAGTTTGGTCAGCCTG
GCTGGATCTCTGTATGCAATTGGTGGTTTTGCTATGATTCAACTGGAGTCTAAAGAATTT
GCACCCACTGAAGTCAATGACATATGGAAGTATGAAGATGATAAAAAAGAATGGGCTGGG
ATGTTGAAGGAAATACGTTATGCTTCAGGAGCTAGTTGCCTAGCAACACGTTTAAATCTC
TTCAAACTGTCTAAACTG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL41
Symbol Nomenclature : KLHL41
SYNONYM : KBTBD10; Krp1; SARCOSIN
Designation : kel-like protein 23|kelch repeat and BTB (POZ) domain containing 10|kelch repeat and BTB domain-containing protein 10|kelch-like 41|kelch-like protein 41|kelch-related protein 1|sarcomeric muscle protein
Full Nomenclature : kelch-like family member 41
GENEID : 10324
GenBank Accession : EU176397
HGNC : 16905
MIM : 607701
Vega : OTTHUMG00000132205
Target GenBank: EU176397

 

Reference Sequence Alignment

  

HsCD00513535      ATGGATTCCCAGCGGGAACTTGCAGAGGAACTGCGGCTTTACCAATCCACCCTTCTTCAG
NM_006063.2       ATGGATTCCCAGCGGGAACTTGCAGAGGAACTGCGGCTTTACCAATCCACCCTTCTTCAG
                  ************************************************************

HsCD00513535      GATGGTCTAAAAGATCTCCTGGATGAGAAAAAATTCATCGATTGCACCCTAAAAGCAGGT
NM_006063.2       GATGGTCTAAAAGATCTCCTGGATGAGAAAAAATTCATCGATTGCACCCTAAAAGCAGGT
                  ************************************************************

HsCD00513535      GACAAAAGTCTTCCTTGCCACAGATTGATTTTGTCAGCTTGTAGTCCTTACTTCCGTGAG
NM_006063.2       GACAAAAGTCTTCCTTGCCACAGATTGATTTTGTCAGCTTGTAGTCCTTACTTCCGTGAG
                  ************************************************************

HsCD00513535      TACTTTTTATCTGAAATTGATGAGGCGAAAAAAAAGGAGGTAGTGCTAGACAATGTGGAT
NM_006063.2       TACTTTTTATCTGAAATTGATGAGGCGAAAAAAAAGGAGGTAGTGCTAGACAATGTGGAT
                  ************************************************************

HsCD00513535      CCTGCTATACTTGATTTAATCATCAAATACCTGTACTCTGCCAGTATTGATCTCAATGAC
NM_006063.2       CCTGCTATACTTGATTTAATCATCAAATACCTGTACTCTGCCAGTATTGATCTCAATGAC
                  ************************************************************

HsCD00513535      GGAAATGTGCAAGATATTTTTGCATTGGCCAGCCGCTTTCAGATCCCCTCAGTGTTTACT
NM_006063.2       GGAAATGTGCAAGATATTTTTGCATTGGCCAGCCGCTTTCAGATCCCCTCAGTGTTTACT
                  ************************************************************

HsCD00513535      GTCTGCGTTTCTTATCTTCAGAAAAGACTTGCTCCTGGTAACTGTCTAGCCATCCTAAGA
NM_006063.2       GTCTGCGTTTCTTATCTTCAGAAAAGACTTGCTCCTGGTAACTGTCTAGCCATCCTAAGA
                  ************************************************************

HsCD00513535      TTAGGACTTCTTCTTGACTGCCCGAGACTCGCCATTTCTGCCCGTGAATTTGTGTCTGAT
NM_006063.2       TTAGGACTTCTTCTTGACTGCCCGAGACTCGCCATTTCTGCCCGTGAATTTGTGTCTGAT
                  ************************************************************

HsCD00513535      CGCTTTGTACAGATTTGTAAGGAAGAGGACTTTATGCAACTGTCTCCACAGGAACTGATC
NM_006063.2       CGCTTTGTACAGATTTGTAAGGAAGAGGACTTTATGCAACTGTCTCCACAGGAACTGATC
                  ************************************************************

HsCD00513535      TCAGTCATTTCAAATGACAGCCTAAATGTAGAAAAAGAAGAAGCAGTATTTGAGGCAGTG
NM_006063.2       TCAGTCATTTCAAATGACAGCCTAAATGTAGAAAAAGAAGAAGCAGTATTTGAGGCAGTG
                  ************************************************************

HsCD00513535      ATGAAATGGGTGCGAACAGACAAGGAAAACAGGGTTAAAAACCTTAGTGAAGTGTTTGAT
NM_006063.2       ATGAAATGGGTGCGAACAGACAAGGAAAACAGGGTTAAAAACCTTAGTGAAGTGTTTGAT
                  ************************************************************

HsCD00513535      TGTATCCGTTTTCGCCTTATGACAGAAAAATATTTTAAGGATCATGTTGAGAAAGATGAT
NM_006063.2       TGTATCCGTTTTCGCCTTATGACAGAAAAATATTTTAAGGATCATGTTGAGAAAGATGAT
                  ************************************************************

HsCD00513535      ATAATTAAAAGCAACCCAGACCTCCAGAAAAAAATCAAAGTTCTAAAAGATGCTTTCGCA
NM_006063.2       ATAATTAAAAGCAACCCAGACCTCCAGAAAAAAATCAAAGTTCTAAAAGATGCTTTCGCA
                  ************************************************************

HsCD00513535      GGCAAACTCCCAGAACCTAGCAAAAATGCCGCGAAGACTGGGGCTGGTGAGGTGAATGGT
NM_006063.2       GGCAAACTCCCAGAACCTAGCAAAAATGCCGCGAAGACTGGGGCTGGTGAGGTGAATGGT
                  ************************************************************

HsCD00513535      GATGTTGGTGATGAAGATTTACTTCCTGGTTACCTGAATGACATTCCCAGGCATGGAATG
NM_006063.2       GATGTTGGTGATGAAGATTTACTTCCTGGTTACCTGAATGACATTCCCAGGCATGGAATG
                  ************************************************************

HsCD00513535      TTTGTAAAAGACCTCATCCTCTTGGTTAATGACACAGCAGCAGTGGCTTATGACCCCACG
NM_006063.2       TTTGTAAAAGACCTCATCCTCTTGGTTAATGACACAGCAGCAGTGGCTTATGACCCCACG
                  ************************************************************

HsCD00513535      GAAAATGAATGCTACCTTACTGCACTGGCTGAGCAGATTCCCAGAAATCATTCCAGCATT
NM_006063.2       GAAAATGAATGCTACCTTACTGCACTGGCTGAGCAGATTCCCAGAAATCATTCCAGCATT
                  ************************************************************

HsCD00513535      GTTACCCAGCAAAATCAGATATATGTGGTAGGAGGACTATATGTGGATGAAGAAAATAAG
NM_006063.2       GTTACCCAGCAAAATCAGATATATGTGGTAGGAGGACTATATGTGGATGAAGAAAATAAG
                  ************************************************************

HsCD00513535      GATCAACCTCTACAGTCATACTTCTTCCAGCTCGATAGCATAGCATCTGAATGGGTTGGA
NM_006063.2       GATCAACCTCTACAGTCATACTTCTTCCAGCTCGATAGCATAGCATCTGAATGGGTTGGA
                  ************************************************************

HsCD00513535      CTTCCACCTCTGCCTTCAGCCAGGTGTCTCTTCGGTCTGGGAGAGGTGGATGATAAAATC
NM_006063.2       CTTCCACCTCTGCCTTCAGCCAGGTGTCTCTTCGGTCTGGGAGAGGTGGATGATAAAATC
                  ************************************************************

HsCD00513535      TATGTAGTTGCAGGCAAAGACCTTCAAACAGAGGCTTCGCTGGATTCAGTATTATGCTAT
NM_006063.2       TATGTAGTTGCAGGCAAAGACCTTCAAACAGAGGCTTCGCTGGATTCAGTATTATGCTAT
                  ************************************************************

HsCD00513535      GATCCTGTGGCTGCAAAATGGAACGAAGTAAAAAAACTCCCTATCAAAGTCTATGGCCAT
NM_006063.2       GATCCTGTGGCTGCAAAATGGAACGAAGTAAAAAAACTCCCTATCAAAGTCTATGGCCAT
                  ************************************************************

HsCD00513535      AATGTGATTTCACATAAAGGGATGATATATTGTCTAGGAGGAAAGACAGATGACAAAAAA
NM_006063.2       AATGTGATTTCACATAAAGGGATGATATATTGTCTAGGAGGAAAGACAGATGACAAAAAA
                  ************************************************************

HsCD00513535      TGTACAAACAGGGTGTTTATCTTCAACCCCAAAAAAGGAGATTGGAAAGATCTGGCTCCA
NM_006063.2       TGTACAAACAGGGTGTTTATCTTCAACCCCAAAAAAGGAGATTGGAAAGATCTGGCTCCA
                  ************************************************************

HsCD00513535      ATGAAAATTCCTCGTTCCATGTTTGGAGTAGCAGTCCATAAAGGCAAAATTGTGATTGCA
NM_006063.2       ATGAAAATTCCTCGTTCCATGTTTGGAGTAGCAGTCCATAAAGGCAAAATTGTGATTGCA
                  ************************************************************

HsCD00513535      GGAGGTGTCACTGAAGATGGTCTTTCAGCTTCAGTTGAAGCTTTTGACCTTACAACAAAT
NM_006063.2       GGAGGTGTCACTGAAGATGGTCTTTCAGCTTCAGTTGAAGCTTTTGACCTTACAACAAAT
                  ************************************************************

HsCD00513535      AAATGGGATGTAATGACCGAATTTCCCCAAGAAAGAAGCTCCATCAGTTTGGTCAGCCTG
NM_006063.2       AAATGGGATGTAATGACCGAATTTCCCCAAGAAAGAAGCTCCATCAGTTTGGTCAGCCTG
                  ************************************************************

HsCD00513535      GCTGGATCTCTGTATGCAATTGGTGGTTTTGCTATGATTCAACTGGAGTCTAAAGAATTT
NM_006063.2       GCTGGATCTCTGTATGCAATTGGTGGTTTTGCTATGATTCAACTGGAGTCTAAAGAATTT
                  ************************************************************

HsCD00513535      GCACCCACTGAAGTCAATGACATATGGAAGTATGAAGATGATAAAAAAGAATGGGCTGGG
NM_006063.2       GCACCCACTGAAGTCAATGACATATGGAAGTATGAAGATGATAAAAAAGAATGGGCTGGG
                  ************************************************************

HsCD00513535      ATGTTGAAGGAAATACGTTATGCTTCAGGAGCTAGTTGCCTAGCAACACGTTTAAATCTC
NM_006063.2       ATGTTGAAGGAAATACGTTATGCTTCAGGAGCTAGTTGCCTAGCAACACGTTTAAATCTC
                  ************************************************************

HsCD00513535      TTCAAACTGTCTAAACTG---
NM_006063.2       TTCAAACTGTCTAAACTGTGA
                  ******************   

Protein

SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : Kelch :
HPRD : 07610

Clone

Cloning Information : 604210
Original Clone ID : ccsbBroadEn_02400

Organism


TAX_ID : 9606
Species Specific ID: 10324

Genome

Chromosome : 2
Map Location : 2q31.1
Ensembl : ENSG00000239474

Reagents

Labome : KLHL41-antibody