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RNF41 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  RNF41
Gene Name:  ring finger protein 41
Sequence             
Original Clone ID: ccsbBroadEn_02346
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC032637.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00513773

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 952nts         Open reading frame : 1 to 952
Reference Sequence Alignment

ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RNF41
Symbol Nomenclature : RNF41
SYNONYM : FLRF; NRDP1
Designation : E3 ubiquitin-protein ligase NRDP1|fetal liver ring finger|neuregulin receptor degradation protein-1
Full Nomenclature : ring finger protein 41
GENEID : 10193
Locus Tag : SBBI03
GenBank Accession : EU446758
HGNC : 18401
Target GenBank: EU446758

 

Reference Sequence Alignment

  

HsCD00513773        ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
NM_001242826.1      ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
NM_005785.3         ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
NM_194359.2         ATGGGGTATGATGTAACCCGTTTCCAGGGGGATGTTGACGAAGATCTTATCTGCCCTATT
                    ************************************************************

HsCD00513773        TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
NM_001242826.1      TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
NM_005785.3         TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
NM_194359.2         TGCAGTGGAGTCTTGGAGGAGCCAGTACAGGCACCTCATTGTGAACATGCTTTCTGCAAC
                    ************************************************************

HsCD00513773        GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
NM_001242826.1      GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
NM_005785.3         GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
NM_194359.2         GCCTGCATCACCCAGTGGTTCTCTCAGCAACAGACATGTCCAGTGGACCGTAGTGTTGTG
                    ************************************************************

HsCD00513773        ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
NM_001242826.1      ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
NM_005785.3         ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
NM_194359.2         ACGGTCGCCCATCTGCGCCCAGTACCTCGGATCATGCGGAACATGTTGTCAAAGCTGCAG
                    ************************************************************

HsCD00513773        ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
NM_001242826.1      ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
NM_005785.3         ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
NM_194359.2         ATTGCCTGTGACAACGCTGTGTTCGGCTGTAGTGCCGTTGTCCGGCTTGACAACCTCATG
                    ************************************************************

HsCD00513773        TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
NM_001242826.1      TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
NM_005785.3         TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
NM_194359.2         TCTCACCTCAGCGACTGTGAGCACAACCCGAAGCGGCCTGTGACCTGTGAACAGGGCTGT
                    ************************************************************

HsCD00513773        GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
NM_001242826.1      GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
NM_005785.3         GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
NM_194359.2         GGCCTGGAGATGCCCAAAGATGAGCTGCCCAACCATAACTGCATTAAGCACCTGCGCTCA
                    ************************************************************

HsCD00513773        GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
NM_001242826.1      GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
NM_005785.3         GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
NM_194359.2         GTGGTACAGCAGCAGCAGACACGCATCGCAGAGCTGGAGAAGACGTCAGCTGAACACAAA
                    ************************************************************

HsCD00513773        CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
NM_001242826.1      CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
NM_005785.3         CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
NM_194359.2         CACCAGCTGGCGGAGCAGAAGCGAGACATCCAGCTGCTAAAGGCATACATGCGTGCAATC
                    ************************************************************

HsCD00513773        CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
NM_001242826.1      CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
NM_005785.3         CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
NM_194359.2         CGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACAATTGAATACAACGAGATCCTA
                    ************************************************************

HsCD00513773        GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
NM_001242826.1      GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
NM_005785.3         GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
NM_194359.2         GAGTGGGTGAACTCCCTTCAGCCAGCAAGAGTGACCCGCTGGGGAGGGATGATCTCGACT
                    ************************************************************

HsCD00513773        CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
NM_001242826.1      CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
NM_005785.3         CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
NM_194359.2         CCTGATGCTGTGCTCCAGGCTGTAATCAAGCGCTCCCTGGTGGAGAGTGGCTGTCCTGCT
                    ************************************************************

HsCD00513773        TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
NM_001242826.1      TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
NM_005785.3         TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
NM_194359.2         TCTATTGTCAACGAGCTGATTGAAAATGCCCACGAGCGTAGCTGGCCCCAGGGTCTGGCC
                    ************************************************************

HsCD00513773        ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
NM_001242826.1      ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
NM_005785.3         ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
NM_194359.2         ACACTAGAGACTAGACAGATGAACCGACGCTACTATGAGAACTACGTGGCCAAGCGCATC
                    ************************************************************

HsCD00513773        CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
NM_001242826.1      CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
NM_005785.3         CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
NM_194359.2         CCTGGCAAGCAGGCTGTTGTCGTGATGGCCTGTGAGAACCAGCACATGGGGGATGACATG
                    ************************************************************

HsCD00513773        GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATA---
NM_001242826.1      GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATAA
NM_005785.3         GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATAA
NM_194359.2         GTGCAAGAGCCAGGCCTTGTCATGATATTTGCGCATGGCGTGGAAGAGATATAA
                    ***************************************************   

Pathways

NCI : ErbB2/ErbB3 signaling events
Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Downregulation of ERBB2:ERBB3 signaling
Reactome : Immune System
Reactome : Signal Transduction
Reactome : Signaling by ERBB2
WikiPathway : Regulation of toll-like receptor signaling pathway

Protein

SMART domain : Ubox : Modified RING finger domain
SMART domain : RING : Ring finger
HPRD : 11520

Clone

Cloning Information : 606283
Original Clone ID : ccsbBroadEn_02346

Organism


TAX_ID : 9606
Species Specific ID: 10193

Genome

Chromosome : 12
Map Location : 12q13.13

Reagents

Labome : RNF41-antibody