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PPIL2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  PPIL2
Gene Name:  peptidylprolyl isomerase (cyclophilin)-like 2
Sequence             
Original Clone ID: ccsbBroadEn_07929
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC000022.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00513881

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1582nts         Open reading frame : 1 to 1582
Reference Sequence Alignment

ATGGGGAAGCGACAGCACCAAAAGGACAAAATGTACATTACCTGTGCTGAATACACTCAC
TTTTATGGTGGCAAGAAGCCAGATCTCCCACAAACAAATTTTCGTCGTTTACCTTTTGAC
CACTGCAGTCTCTCTCTGCAGCCCTTTGTCTACCCAGTCTGCACTCCCGATGGCATCGTC
TTTGACTTACTGAACATTGTTCCATGGCTTAAGAAGTACGGGACCAACCCCAGCAATGGA
GAGAAGCTGGACGGGAGGTCCCTGATCAAGCTGAACTTTTCCAAGAACAGTGAGGGGAAG
TACCACTGCCCAGTGCTGTTTACCGTGTTCACCAACAACACCCACATCGTGGCTGTGAGG
ACGACCGGCAACGTCTACGCCTATGAGGCAGTGGAACAGCTAAATATCAAGGCCAAGAAC
TTCCGGGACCTGCTGACCGACGAGCCCTTCTCCCGGCAGGACATCATCACCCTCCAGGAC
CCCACCAATTTGGACAAGTTCAATGTCTCTAACTTCTATCATGTGAAGAATAACATGAAA
ATAATAGACCCAGATGAAGAGAAGGCCAAACAGGACCCGTCTTATTATCTGAAAAATACA
AATGCCGAGACCCGAGAGACCCTGCAGGAGCTCTACAAGGAGTTCAAAGGGGACGAGATT
CTGGCAGCCACCATGAAGGCCCCGGAGAAGAAGAAAGTGGACAAGCTGAATGCTGCCCAC
TATTCCACAGGGAAGGTCAGCGCTTCCTTCACCTCCACCGCGATGGTCCCGGAGACCACA
CATGAAGCAGCTGCCATCGACGAGGATGTGCTGCGCTACCAGTTTGTGAAGAAGAAGGGC
TACGTGCGGCTGCACACCAACAAGGGCGACCTCAACCTGGAGCTGCACTGCGACCTGACA
CCAAAAACCTGCGAAAACTTCATCAGGCTTTGCAAGAAGCATTATTACGATGGCACCATC
TTCCACAGATCCATCCGGAACTTTGTGATCCAAGGGGGCGACCCCACAGGCACAGGCACG
GGTGGGGAGTCATACTGGGGGAAGCCCTTCAAAGACGAGTTCCGGCCCAACCTCTCGCAC
ACGGGCCGCGGCATCCTCAGCATGGCCAACTCCGGGCCCAACAGCAACAGGTCTCAATTC
TTCATCACGTTTCGCTCCTGTGCCTACCTGGACAAGAAGCATACCATCTTTGGACGGGTT
GTTGGGGGCTTTGACGTACTGACAGCCATGGAGAATGTGGAGAGTGACCCCAAAACTGAC
CGCCCTAAGGAGGAGATCCGCATTGATGCCACTACAGTGTTCGTGGACCCCTATGAGGAG
GCCGATGCCCAGATTGCGCAGGAGCGGAAGACACAGCTCAAGGTAGCCCCGGAGACCAAA
GTGAAGAGCAGCCAGCCCCAGGCAGGGAGCCAGGGCCCCCAGACCTTCCGCCAGGGCGTG
GGCAAGTACATCAACCCAGCAGCCACCGAGCAGCAGAGGAAGAGCCCTCAACCAGTGCCA
CTGTCCCCATGTCCAAGAAGAAGCCCAGTCGGGGTTTTGGGGACTTCAGCTCCTGGTAGC
AGCAGGCTGCCTGATGACCAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : PPIL2
Symbol Nomenclature : PPIL2
SYNONYM : CYC4; CYP60; Cyp-60; UBOX7; hCyP-60
Designation : PPIase|U-box domain containing 7|cyclophilin, 60kDa|cyclophilin-60|cyclophilin-like protein CyP-60|peptidyl-prolyl cis-trans isomerase-like 2|peptidylprolyl cis-trans isomerase|rotamase PPIL2
Full Nomenclature : peptidylprolyl isomerase (cyclophilin)-like 2
GENEID : 23759
GenBank Accession : DQ894846
HGNC : 9261
MIM : 607588
Vega : OTTHUMG00000030174
Target GenBank: DQ894846

 

Reference Sequence Alignment

  

HsCD00513881      ATGGGGAAGCGACAGCACCAAAAGGACAAAATGTACATTACCTGTGCTGAATACACTCAC
NM_148175.2       ATGGGGAAGCGACAGCACCAAAAGGACAAAATGTACATTACCTGTGCTGAATACACTCAC
NM_148176.2       ATGGGGAAGCGACAGCACCAAAAGGACAAAATGTACATTACCTGTGCTGAATACACTCAC
NM_014337.3       ATGGGGAAGCGACAGCACCAAAAGGACAAAATGTACATTACCTGTGCTGAATACACTCAC
                  ************************************************************

HsCD00513881      TTTTATGGTGGCAAGAAGCCAGATCTCCCACAAACAAATTTTCGTCGTTTACCTTTTGAC
NM_148175.2       TTTTATGGTGGCAAGAAGCCAGATCTCCCACAAACAAATTTTCGTCGTTTACCTTTTGAC
NM_148176.2       TTTTATGGTGGCAAGAAGCCAGATCTCCCACAAACAAATTTTCGTCGTTTACCTTTTGAC
NM_014337.3       TTTTATGGTGGCAAGAAGCCAGATCTCCCACAAACAAATTTTCGTCGTTTACCTTTTGAC
                  ************************************************************

HsCD00513881      CACTGCAGTCTCTCTCTGCAGCCCTTTGTCTACCCAGTCTGCACTCCCGATGGCATCGTC
NM_148175.2       CACTGCAGTCTCTCTCTGCAGCCCTTTGTCTACCCAGTCTGCACTCCCGATGGCATCGTC
NM_148176.2       CACTGCAGTCTCTCTCTGCAGCCCTTTGTCTACCCAGTCTGCACTCCCGATGGCATCGTC
NM_014337.3       CACTGCAGTCTCTCTCTGCAGCCCTTTGTCTACCCAGTCTGCACTCCCGATGGCATCGTC
                  ************************************************************

HsCD00513881      TTTGACTTACTGAACATTGTTCCATGGCTTAAGAAGTACGGGACCAACCCCAGCAATGGA
NM_148175.2       TTTGACTTACTGAACATTGTTCCATGGCTTAAGAAGTACGGGACCAACCCCAGCAATGGA
NM_148176.2       TTTGACTTACTGAACATTGTTCCATGGCTTAAGAAGTACGGGACCAACCCCAGCAATGGA
NM_014337.3       TTTGACTTACTGAACATTGTTCCATGGCTTAAGAAGTACGGGACCAACCCCAGCAATGGA
                  ************************************************************

HsCD00513881      GAGAAGCTGGACGGGAGGTCCCTGATCAAGCTGAACTTTTCCAAGAACAGTGAGGGGAAG
NM_148175.2       GAGAAGCTGGACGGGAGGTCCCTGATCAAGCTGAACTTTTCCAAGAACAGTGAGGGGAAG
NM_148176.2       GAGAAGCTGGACGGGAGGTCCCTGATCAAGCTGAACTTTTCCAAGAACAGTGAGGGGAAG
NM_014337.3       GAGAAGCTGGACGGGAGGTCCCTGATCAAGCTGAACTTTTCCAAGAACAGTGAGGGGAAG
                  ************************************************************

HsCD00513881      TACCACTGCCCAGTGCTGTTTACCGTGTTCACCAACAACACCCACATCGTGGCTGTGAGG
NM_148175.2       TACCACTGCCCAGTGCTGTTTACCGTGTTCACCAACAACACCCACATCGTGGCTGTGAGG
NM_148176.2       TACCACTGCCCAGTGCTGTTTACCGTGTTCACCAACAACACCCACATCGTGGCTGTGAGG
NM_014337.3       TACCACTGCCCAGTGCTGTTTACCGTGTTCACCAACAACACCCACATCGTGGCTGTGAGG
                  ************************************************************

HsCD00513881      ACGACCGGCAACGTCTACGCCTATGAGGCAGTGGAACAGCTAAATATCAAGGCCAAGAAC
NM_148175.2       ACGACCGGCAACGTCTACGCCTATGAGGCAGTGGAACAGCTAAATATCAAGGCCAAGAAC
NM_148176.2       ACGACCGGCAACGTCTACGCCTATGAGGCAGTGGAACAGCTAAATATCAAGGCCAAGAAC
NM_014337.3       ACGACCGGCAACGTCTACGCCTATGAGGCAGTGGAACAGCTAAATATCAAGGCCAAGAAC
                  ************************************************************

HsCD00513881      TTCCGGGACCTGCTGACCGACGAGCCCTTCTCCCGGCAGGACATCATCACCCTCCAGGAC
NM_148175.2       TTCCGGGACCTGCTGACCGACGAGCCCTTCTCCCGGCAGGACATCATCACCCTCCAGGAC
NM_148176.2       TTCCGGGACCTGCTGACCGACGAGCCCTTCTCCCGGCAGGACATCATCACCCTCCAGGAC
NM_014337.3       TTCCGGGACCTGCTGACCGACGAGCCCTTCTCCCGGCAGGACATCATCACCCTCCAGGAC
                  ************************************************************

HsCD00513881      CCCACCAATTTGGACAAGTTCAATGTCTCTAACTTCTATCATGTGAAGAATAACATGAAA
NM_148175.2       CCCACCAATTTGGACAAGTTCAATGTCTCTAACTTCTATCATGTGAAGAATAACATGAAA
NM_148176.2       CCCACCAATTTGGACAAGTTCAATGTCTCTAACTTCTATCATGTGAAGAATAACATGAAA
NM_014337.3       CCCACCAATTTGGACAAGTTCAATGTCTCTAACTTCTATCATGTGAAGAATAACATGAAA
                  ************************************************************

HsCD00513881      ATAATAGACCCAGATGAAGAGAAGGCCAAACAGGACCCGTCTTATTATCTGAAAAATACA
NM_148175.2       ATAATAGACCCAGATGAAGAGAAGGCCAAACAGGACCCGTCTTATTATCTGAAAAATACA
NM_148176.2       ATAATAGACCCAGATGAAGAGAAGGCCAAACAGGACCCGTCTTATTATCTGAAAAATACA
NM_014337.3       ATAATAGACCCAGATGAAGAGAAGGCCAAACAGGACCCGTCTTATTATCTGAAAAATACA
                  ************************************************************

HsCD00513881      AATGCCGAGACCCGAGAGACCCTGCAGGAGCTCTACAAGGAGTTCAAAGGGGACGAGATT
NM_148175.2       AATGCCGAGACCCGAGAGACCCTGCAGGAGCTCTACAAGGAGTTCAAAGGGGACGAGATT
NM_148176.2       AATGCCGAGACCCGAGAGACCCTGCAGGAGCTCTACAAGGAGTTCAAAGGGGACGAGATT
NM_014337.3       AATGCCGAGACCCGAGAGACCCTGCAGGAGCTCTACAAGGAGTTCAAAGGGGACGAGATT
                  ************************************************************

HsCD00513881      CTGGCAGCCACCATGAAGGCCCCGGAGAAGAAGAAAGTGGACAAGCTGAATGCTGCCCAC
NM_148175.2       CTGGCAGCCACCATGAAGGCCCCGGAGAAGAAGAAAGTGGACAAGCTGAACGCTGCCCAC
NM_148176.2       CTGGCAGCCACCATGAAGGCCCCGGAGAAGAAGAAAGTGGACAAGCTGAACGCTGCCCAC
NM_014337.3       CTGGCAGCCACCATGAAGGCCCCGGAGAAGAAGAAAGTGGACAAGCTGAACGCTGCCCAC
                  ************************************************** *********

HsCD00513881      TATTCCACAGGGAAGGTCAGCGCTTCCTTCACCTCCACCGCGATGGTCCCGGAGACCACA
NM_148175.2       TATTCCACAGGGAAGGTCAGCGCTTCCTTCACCTCCACCGCGATGGTCCCGGAGACCACA
NM_148176.2       TATTCCACAGGGAAGGTCAGCGCTTCCTTCACCTCCACCGCGATGGTCCCGGAGACCACA
NM_014337.3       TATTCCACAGGGAAGGTCAGCGCTTCCTTCACCTCCACCGCGATGGTCCCGGAGACCACA
                  ************************************************************

HsCD00513881      CATGAAGCAGCTGCCATCGACGAGGATGTGCTGCGCTACCAGTTTGTGAAGAAGAAGGGC
NM_148175.2       CATGAAGCAGCTGCCATCGACGAGGATGTGCTGCGCTACCAGTTTGTGAAGAAGAAGGGC
NM_148176.2       CATGAAGCAGCTGCCATCGACGAGGATGTGCTGCGCTACCAGTTTGTGAAGAAGAAGGGC
NM_014337.3       CATGAAGCAGCTGCCATCGACGAGGATGTGCTGCGCTACCAGTTTGTGAAGAAGAAGGGC
                  ************************************************************

HsCD00513881      TACGTGCGGCTGCACACCAACAAGGGCGACCTCAACCTGGAGCTGCACTGCGACCTGACA
NM_148175.2       TACGTGCGGCTGCACACCAACAAGGGCGACCTCAACCTGGAGCTGCACTGCGACCTGACA
NM_148176.2       TACGTGCGGCTGCACACCAACAAGGGCGACCTCAACCTGGAGCTGCACTGCGACCTGACA
NM_014337.3       TACGTGCGGCTGCACACCAACAAGGGCGACCTCAACCTGGAGCTGCACTGCGACCTGACA
                  ************************************************************

HsCD00513881      CCAAAAACCTGCGAAAACTTCATCAGGCTTTGCAAGAAGCATTATTACGATGGCACCATC
NM_148175.2       CCAAAAACCTGCGAAAACTTCATCAGGCTTTGCAAGAAGCATTATTACGATGGCACCATC
NM_148176.2       CCAAAAACCTGCGAAAACTTCATCAGGCTTTGCAAGAAGCATTATTACGATGGCACCATC
NM_014337.3       CCAAAAACCTGCGAAAACTTCATCAGGCTTTGCAAGAAGCATTATTACGATGGCACCATC
                  ************************************************************

HsCD00513881      TTCCACAGATCCATCCGGAACTTTGTGATCCAAGGGGGCGACCCCACAGGCACAGGCACG
NM_148175.2       TTCCACAGATCCATCCGGAACTTTGTGATCCAAGGGGGCGACCCCACAGGCACAGGCACG
NM_148176.2       TTCCACAGATCCATCCGGAACTTTGTGATCCAAGGGGGCGACCCCACAGGCACAGGCACG
NM_014337.3       TTCCACAGATCCATCCGGAACTTTGTGATCCAAGGGGGCGACCCCACAGGCACAGGCACG
                  ************************************************************

HsCD00513881      GGTGGGGAGTCATACTGGGGGAAGCCCTTCAAAGACGAGTTCCGGCCCAACCTCTCGCAC
NM_148175.2       GGTGGGGAGTCATACTGGGGGAAGCCCTTCAAAGACGAGTTCCGGCCCAACCTCTCGCAC
NM_148176.2       GGTGGGGAGTCATACTGGGGGAAGCCCTTCAAAGACGAGTTCCGGCCCAACCTCTCGCAC
NM_014337.3       GGTGGGGAGTCATACTGGGGGAAGCCCTTCAAAGACGAGTTCCGGCCCAACCTCTCGCAC
                  ************************************************************

HsCD00513881      ACGGGCCGCGGCATCCTCAGCATGGCCAACTCCGGGCCCAACAGCAACAGGTCTCAATTC
NM_148175.2       ACGGGCCGCGGCATCCTCAGCATGGCCAACTCCGGGCCCAACAGCAACAGGTCTCAATTC
NM_148176.2       ACGGGCCGCGGCATCCTCAGCATGGCCAACTCCGGGCCCAACAGCAACAGGTCTCAATTC
NM_014337.3       ACGGGCCGCGGCATCCTCAGCATGGCCAACTCCGGGCCCAACAGCAACAGGTCTCAATTC
                  ************************************************************

HsCD00513881      TTCATCACGTTTCGCTCCTGTGCCTACCTGGACAAGAAGCATACCATCTTTGGACGGGTT
NM_148175.2       TTCATCACGTTTCGCTCCTGTGCCTACCTGGACAAGAAGCATACCATCTTTGGACGGGTT
NM_148176.2       TTCATCACGTTTCGCTCCTGTGCCTACCTGGACAAGAAGCATACCATCTTTGGACGGGTT
NM_014337.3       TTCATCACGTTTCGCTCCTGTGCCTACCTGGACAAGAAGCATACCATCTTTGGACGGGTT
                  ************************************************************

HsCD00513881      GTTGGGGGCTTTGACGTACTGACAGCCATGGAGAATGTGGAGAGTGACCCCAAAACTGAC
NM_148175.2       GTTGGGGGCTTTGACGTACTGACAGCCATGGAGAATGTGGAGAGTGACCCCAAAACTGAC
NM_148176.2       GTTGGGGGCTTTGACGTACTGACAGCCATGGAGAATGTGGAGAGTGACCCCAAAACTGAC
NM_014337.3       GTTGGGGGCTTTGACGTACTGACAGCCATGGAGAATGTGGAGAGTGACCCCAAAACTGAC
                  ************************************************************

HsCD00513881      CGCCCTAAGGAGGAGATCCGCATTGATGCCACTACAGTGTTCGTGGACCCCTATGAGGAG
NM_148175.2       CGCCCTAAGGAGGAGATCCGCATTGATGCCACTACAGTGTTCGTGGACCCCTATGAGGAG
NM_148176.2       CGCCCTAAGGAGGAGATCCGCATTGATGCCACTACAGTGTTCGTGGACCCCTATGAGGAG
NM_014337.3       CGCCCTAAGGAGGAGATCCGCATTGATGCCACTACAGTGTTCGTGGACCCCTATGAGGAG
                  ************************************************************

HsCD00513881      GCCGATGCCCAGATTGCGCAGGAGCGGAAGACACAGCTCAAGGTAGCCCCGGAGACCAAA
NM_148175.2       GCCGATGCCCAGATTGCGCAGGAGCGGAAGACACAGCTCAAGGTAGCCCCGGAGACCAAA
NM_148176.2       GCCGATGCCCAGATTGCGCAGGAGCGGAAGACACAGCTCAAGGTAGCCCCGGAGACCAAA
NM_014337.3       GCCGATGCCCAGATTGCGCAGGAGCGGAAGACACAGCTCAAGGTAGCCCCGGAGACCAAA
                  ************************************************************

HsCD00513881      GTGAAGAGCAGCCAGCCCCAGGCAGGGAGCCAGGGCCCCCAGACCTTCCGCCAGGGCGTG
NM_148175.2       GTGAAGAGCAGCCAGCCCCAGGCAGGGAGCCAGGGCCCCCAGACCTTCCGCCAGGGCGTG
NM_148176.2       GTGAAGAGCAGCCAGCCCCAGGCAGGGAGCCAGGGCCCCCAGACCTTCCGCCAGGGCGTG
NM_014337.3       GTGAAGAGCAGCCAGCCCCAGGCAGGGAGCCAGGGCCCCCAGACCTTCCGCCAGGGCGTG
                  ************************************************************

HsCD00513881      GGCAAGTACATCAACCCAGCAGCCACCGAGCAG----CAGAGGAAGAGCCCTCAACCAGT
NM_148175.2       GGCAAGTACATCAACCCAGCAGCCACGAAGCGAGCAGCAGAGGAAGAGCCCTCAACCAGT
NM_148176.2       GGCAAGTACATCAACCCAGCAGCCACCGAGCAG----CAGAGGAAGAGCCCTCAACCAGT
NM_014337.3       GGCAAGTACATCAACCCAGCAGCCACGAAGCGAGCAGCAGAGGAAGAGCCCTCAACCAGT
                  ************************** .***..    ***********************

HsCD00513881      GCCACTGTCCCCATGTCCAAGAAGAAGCCCAGTCGGGGTTTTGGGGACTTCAGCTCCTGG
NM_148175.2       GCCACTGTCCCCATGTCCAAGAAGAAGCCCAGTCGGGGTTTTGGGGACTTCAGCTCCTGG
NM_148176.2       GCCACTGTCCCCATGTCCAAGAAGAAGCCCAGTCGGGGTTTTGGGGACTTCAGCTCCTGG
NM_014337.3       GCCACTGTCCCCATGTCCAAGAAGAAGCCCAGTCGGGGTTTTGGGGACTTCAGCTCCTGG
                  ************************************************************

HsCD00513881      TAGCAGCAGGCTGCCTGATGACCAC---
NM_148175.2       TAG-------------------------
NM_148176.2       TAGCAGCAGGCTGCCTGATGACCACTAG
NM_014337.3       TAG-------------------------
                  ***                         

Pathways

Reactome : Basigin interactions
Reactome : Cell surface interactions at the vascular wall
Reactome : Hemostasis

Protein

SMART domain : Ubox : Modified RING finger domain
HPRD : 08472

Clone

Cloning Information : 606109
Original Clone ID : ccsbBroadEn_07929

Organism


TAX_ID : 9606
Species Specific ID: 23759

Genome

Chromosome : 22
Map Location : 22q11.21
Ensembl : ENSG00000100023

Reagents

Labome : PPIL2-antibody