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UBOX5 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  UBOX5
Gene Name:  U-box domain containing 5
Sequence             
Original Clone ID: ccsbBroadEn_02697
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC000515.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00513927

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1624nts         Open reading frame : 1 to 1624
Reference Sequence Alignment

ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCA
GCTGATGGTTACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGT
TTCAGGACAGAGTATTTCATTAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAAT
GTGGAAATCTGTAGGATCAACATAGACCTCACAGCTGGGGGAGGTCAGAACGTCACTGGC
CTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTTGGAATACGCCCCAGTGCCGG
ACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTTGGTAGGCAAA
GTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC
CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGG
AATAAAGGGGCTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTG
ACAGGCGGCGGTATCCCTTGTATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACC
TGCTCCCAGGAAGTGATAGACAGCATCCTGCTGGTCACCTCAGAGAACCTGCCTCAGGAT
GTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACTGTGACCCTGGGGACCAGCCT
GAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCAGGATGTGCCT
GAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA
GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGC
CGAGTGCCCAGTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCT
CACCCCTCCCTCAAGGCCCGGATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGC
CACCTGCTTGGGAGAGCACAGACGGCATTGGCAGTGATCCCTTCTTCCATTGTTCTGCCC
TCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAGACAGTAACTTTGGTGTAAAT
GCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGAGCACACTGCT
AAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA
CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGC
TCTATGCCCTCCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGA
GGGAGCAACACTTCCTGGAGGCCTGGCACCGGCTCGGAGCAGCCTGGGAGCATCCTGGGC
CCCGAATGTGCCTCCTGCAAAAGAGTATTTTCTCCCTACTTCAAAAAGGAGCCGGTGTAC
CAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGGGTGAGAAGCAACGCTCCCTG
CCCATGACGTGCACAGCCTGCCAGCGGCCGGTTGCTAGCCAAGACGTGCTGCGGGTCCAC
TTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : UBOX5
Symbol Nomenclature : UBOX5
SYNONYM : RNF37; UBCE7IP5; UIP5
Designation : RING finger protein 37|U-box domain-containing protein 5|ubiquitin conjugating enzyme 7 interacting protein 5|ubiquitin-conjugating enzyme 7-interacting protein 5
Full Nomenclature : U-box domain containing 5
GENEID : 22888
Locus Tag : RP5-1187M17.9
GenBank Accession : DQ895205
HGNC : 17777
Vega : OTTHUMG00000031731
Target GenBank: DQ895205

 

Reference Sequence Alignment

  

HsCD00513927        ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCA
NM_001267584.1      ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCA
NM_014948.3         ATGGTAATAAATCTTTGCCTCCCACAGTTCAGACCAAGAATTCACTGCAACAAGATATCA
                    ************************************************************

HsCD00513927        GCTGATGGTTACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGT
NM_001267584.1      GCTGATGGTTACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGT
NM_014948.3         GCTGATGGTTACGAAGTAGAAAATCTCATCTCTGAAGATCTCACAAAGAGAAGTCATGGT
                    ************************************************************

HsCD00513927        TTCAGGACAGAGTATTTCATTAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAAT
NM_001267584.1      TTCAGGACAGAGTATTTCATTAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAAT
NM_014948.3         TTCAGGACAGAGTATTTCATTAAGCCACCAGTCTATGTGACAGTTTCATTTCCCTTTAAT
                    ************************************************************

HsCD00513927        GTGGAAATCTGTAGGATCAACATAGACCTCACAGCTGGGGGAGGTCAGAACGTCACTGGC
NM_001267584.1      GTGGAAATCTGTAGGATCAACATAGACCTCACAGCTGGGGGAGGTCAGAACGTCACTGGC
NM_014948.3         GTGGAAATCTGTAGGATCAACATAGACCTCACAGCTGGGGGAGGTCAGAACGTCACTGGC
                    ************************************************************

HsCD00513927        CTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTTGGAATACGCCCCAGTGCCGG
NM_001267584.1      CTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTTGGAATACGCCCCAGTGCCGG
NM_014948.3         CTGGAAATGTACACATCTGCCTCATCTAGCAGAGTGTCTTGGAATACGCCCCAGTGCCGG
                    ************************************************************

HsCD00513927        ACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTTGGTAGGCAAA
NM_001267584.1      ACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTTGGTAGGCAAA
NM_014948.3         ACCCTGGGCCCAGCTGAGCCATCTGTCCCAGACAAGGAGGCGTTCACCTTGGTAGGCAAA
                    ************************************************************

HsCD00513927        GTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC
NM_001267584.1      GTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC
NM_014948.3         GTCTTACTGAAAAACCAGAGCCAAGTGGTGTTTAGCCACAGGGGCTTCAAGGCCAGGCCC
                    ************************************************************

HsCD00513927        CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGG
NM_001267584.1      CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGG
NM_014948.3         CCTTTTGGCGCGATGGAAGCCACACTCCCCTCCCCTGCTGTTGTGGCCCAGGAGCTCTGG
                    ************************************************************

HsCD00513927        AATAAAGGGGCTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTG
NM_001267584.1      AATAAAGGGGCTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTG
NM_014948.3         AATAAAGGGGCTCTTTCCCTTAGCCACGTGGCCCACTTAAGGATCTGTATCACCCATGTG
                    ************************************************************

HsCD00513927        ACAGGCGGCGGTATCCCTTGTATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACC
NM_001267584.1      ACAGGCGGCGGTATCCCTTGTATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACC
NM_014948.3         ACAGGCGGCGGTATCCCTTGTATCAAGCGGTTGGAAGTGTGGGGTCAGCCGGCCAAGACC
                    ************************************************************

HsCD00513927        TGCTCCCAGGAAGTGATAGACAGCATCCTGCTGGTCACCTCAGAGAACCTGCCTCAGGAT
NM_001267584.1      TGCTCCCAGGAAGTGATAGACAGCATCCTGCTGGTCACCTCAGAGAACCTGCCTCAGGAT
NM_014948.3         TGCTCCCAGGAAGTGATAGACAGCATCCTGCTGGTCACCTCAGAGAACCTGCCTCAGGAT
                    ************************************************************

HsCD00513927        GTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACTGTGACCCTGGGGACCAGCCT
NM_001267584.1      GTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACTGTGACCCTGGGGACCAGCCT
NM_014948.3         GTGGCTCTGCAGGCTCCAGCCTTGCCCATGGAAAGTGACTGTGACCCTGGGGACCAGCCT
                    ************************************************************

HsCD00513927        GAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCAGGATGTGCCT
NM_001267584.1      GAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCAGGATGTGCCT
NM_014948.3         GAGAGCCAGCAGGCTCCCTCCAGCCTGCAGAAGCTGGCCGAGATCATTCAGGATGTGCCT
                    ************************************************************

HsCD00513927        GAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA
NM_001267584.1      GAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA
NM_014948.3         GAGGAGTTCCTGGATCCCATCACCCTGGAGATCATGCCTTGTCCCATGCTGCTGCCCTCA
                    ************************************************************

HsCD00513927        GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGC
NM_001267584.1      GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGC
NM_014948.3         GGCAAGGTCATCGACCAGAGCACACTGGAGAAGTGTAACCGCAGTGAAGCCACATGGGGC
                    ************************************************************

HsCD00513927        CGAGTGCCCAGTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCT
NM_001267584.1      CGAGTGCCCAGTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCT
NM_014948.3         CGAGTGCCCAGTGACCCTTTCACGGGGGTAGCTTTTACTCCGCACTCTCAGCCCCTGCCT
                    ************************************************************

HsCD00513927        CACCCCTCCCTCAAGGCCCGGATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGC
NM_001267584.1      CACCCCTCCCTCAAGGCCCGGATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGC
NM_014948.3         CACCCCTCCCTCAAGGCCCGGATTGACCATTTCCTGCTCCAGCACTCCATCCCTGGCTGC
                    ************************************************************

HsCD00513927        CACCTGCTTGGGAGAGCACAGACGGCATTGGCAGTGATCCCTTCTTCCATTGTTCTGCCC
NM_001267584.1      CACCTGCTTGGGAGAGCACAGACGGCATTGGCAGTGATCCCTTCTTCCATTGTTCTGCCC
NM_014948.3         CACCTGCTTGGGAGAGCACAGACGGCATTGGCAGTGATCCCTTCTTCCATTGTTCTGCCC
                    ************************************************************

HsCD00513927        TCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAGACAGTAACTTTGGTGTAAAT
NM_001267584.1      TCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAGACAGTAACTTTGGTGTAAAT
NM_014948.3         TCTCAGAAAAGGAAGATAGAGCAGGCTGAACATGTCCCAGACAGTAACTTTGGTGTAAAT
                    ************************************************************

HsCD00513927        GCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGAGCACACTGCT
NM_001267584.1      GCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGAGCACACTGCT
NM_014948.3         GCTTCCTGTTTTTCTGCCACAAGCCCTTTGGTCTTACCCACTACCTCAGAGCACACTGCT
                    ************************************************************

HsCD00513927        AAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA
NM_001267584.1      AAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA
NM_014948.3         AAGAAAATGAAAGCCACCAATGAGCCCAGCCTGACACATATGGACTGCTCGACAGGTCCA
                    ************************************************************

HsCD00513927        CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGC
NM_001267584.1      CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGC
NM_014948.3         CTGTCCCACGAGCAGAAGCTGTCACAAAGCTTGGAAATTGCCTTGGCATCCACCCTTGGC
                    ************************************************************

HsCD00513927        TCTATGCCCTCCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGA
NM_001267584.1      TCTATGCCCTCCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGA
NM_014948.3         TCTATGCCCTCCTTCACGGCACGGCTGACCAGGGGACAGCTCCAGCACCTTGGCACAAGA
                    ************************************************************

HsCD00513927        GGGAGCAACACTTCCTGGAGGCCTGGCACCGGCTCGGAGCAGCCTGGGAGCATCCTGGGC
NM_001267584.1      GGGAGCAACACTTCCTGGAGGCCTGGCACCGGCTCG-----GCCTGGGAGCATCCTGGGC
NM_014948.3         GGGAGCAACACTTCCTGGAGGCCTGGCACCGGCTCGGAGCAGCCTGGGAGCATCCTGGGC
                    ************************************     *******************

HsCD00513927        CCCGAATGTGCCTCCTGCAAAAGAGTATTTTCTCCCTACTTCAAAAAGGAGCCGGTGTAC
NM_001267584.1      CCCGAATGTGCCTCCTGCAAAAGAGTATTTTCTCCCTACTTCAAAAAGGAGCCGGTGTAC
NM_014948.3         CCCGAATGTGCCTCCTGCAAAAGAGTATTTTCTCCCTACTTCAAAAAGGAGCCGGTGTAC
                    ************************************************************

HsCD00513927        CAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGGGTGAGAAGCAACGCTCCCTG
NM_001267584.1      CAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGGGTGA----------------
NM_014948.3         CAGCTGCCCTGCGGCCACCTCCTGTGCCGACCCTGCCTGGGTGAGAAGCAACGCTCCCTG
                    ********************************************                

HsCD00513927        CCCATGACGTGCACAGCCTGCCAGCGGCCGGTTGCTAGCCAAGACGTGCTGCGGGTCCAC
NM_001267584.1      ------------------------------------------------------------
NM_014948.3         CCCATGACGTGCACAGCCTGCCAGCGGCCGGTTGCTAGCCAAGACGTGCTGCGGGTCCAC
                                                                                

HsCD00513927        TTC---
NM_001267584.1      ------
NM_014948.3         TTCTGA
                          

Pathways

Reactome : Adaptive Immune System
Reactome : Antigen processing: Ubiquitination & Proteasome degradation
Reactome : Class I MHC mediated antigen processing & presentation
Reactome : Immune System

Protein

SMART domain : Ubox : Modified RING finger domain
HPRD : 15605

Clone

Cloning Information : 606155
Original Clone ID : ccsbBroadEn_02697

Organism


TAX_ID : 9606
Species Specific ID: 22888

Genome

Chromosome : 20
Map Location : 20p13
Ensembl : ENSG00000185019

Reagents

Labome : UBOX5-antibody