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KLHL2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL2
Gene Name:  kelch-like family member 2
Sequence             
Original Clone ID: ccsbBroadEn_07792
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC036468.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00514064

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1780nts         Open reading frame : 1 to 1780
Reference Sequence Alignment

ATGGAGACGCCGCCGCTGCCTCCCGCATGCACAAAGCAGGGTCATCAGAAGCCTCTCGAT
TCAAAAGATGATAATACCGAAAAACACTGCCCAGTGACAGTGAATCCTTGGCATATGAAG
AAAGCTTTCAAAGTCATGAACGAATTAAGAAGTCAAAATTTGCTGTGCGATGTCACAATT
GTGGCAGAAGACATGGAAATTTCTGCTCATAGAGTGGTGCTGGCCGCCTGTAGTCCTTAT
TTTCATGCCATGTTTACAGGTGAGATGAGTGAGAGCCGAGCAAAGAGAGTTAGAATAAAA
GAGGTAGATGGCTGGACCCTGAGGATGCTAATTGATTATGTTTACACTGCAGAAATTCAG
GTTACAGAAGAAAATGTACAGGTACTTCTCCCAGCAGCTGGTCTCTTACAGTTACAGGAT
GTGAAGAAGACTTGTTGTGAATTTTTGGAATCCCAGCTTCACCCTGTCAACTGCTTAGGA
ATCCGGGCTTTTGCTGATATGCATGCATGTACCGACCTTCTGAACAAGGCCAACACCTAT
GCAGAGCAACATTTTGCAGATGTTGTACTTAGTGAAGAATTTCTCAATCTTGGCATCGAA
CAAGTGTGCAGCTTAATCTCAAGTGACAAACTTACCATTTCTTCAGAAGAGAAGGTATTT
GAAGCAGTAATAGCATGGGTGAACCATGACAAGGATGTGAGGCAAGAGTTTATGGCCCGA
CTGATGGAACATGTACGGTTACCTTTGCTTCCTCGGGAATATTTAGTTCAGAGGGTTGAA
GAGGAAGCATTGGTCAAGAATAGCAGTGCTTGCAAAGATTACCTCATTGAAGCAATGAAG
TACCATTTGCTGCCAACAGAGCAGCGTATATTAATGAAGAGTGTCCGGACCCGGCTGAGG
ACACCCATGAACCTTCCCAAATTGATGGTGGTGGTTGGGGGCCAAGCACCAAAGGCTATC
CGGAGTGTGGAATGCTATGACTTTAAAGAAGAAAGGTGGCACCAAGTAGCAGAGTTGCCT
TCCGGGAGGTGCAGGGCAGGCATGGTCTACATGGCTGGACTTGTTTTTGCTGTTGGTGGC
TTTAATGGCTCATTAAGAGTTCGCACTGTAGATTCCTACGACCCTGTGAAGGACCAGTGG
ACCAGCGTTGCTAACATGAGAGACCGGAGAAGCACTTTGGGAGCTGCTGTGTTAAATGGA
TTATTATACGCTGTGGGAGGCTTTGATGGGAGTACAGGTTTGTCATCTGTGGAAGCATAC
AACATAAAGTCTAATGAGTGGTTTCATGTAGCTCCCATGAATACAAGGAGGAGCAGTGTT
GGTGTGGGTGTTGTTGGAGGTTTGCTCTATGCTGTAGGAGGTTATGATGGAGCATCACGT
CAGTGTCTTAGCACAGTAGAATGCTATAATGCTACAACAAATGAGTGGACCTATATAGCA
GAAATGAGCACCAGGCGGAGTGGAGCAGGTGTTGGTGTGTTAAACAATTTATTGTATGCT
GTAGGAGGTCATGATGGCCCTTTAGTACGAAAAAGTGTTGAAGTATATGATCCCACCACT
AACGCATGGAGACAGGTTGCAGATATGAACATGTGCAGAAGAAATGCAGGAGTTTGTGCA
GTTAATGGTCTGTTATATGTTGTTGGAGGGGATGATGGTTCCTGTAACTTGGCGTCAGTA
GAATATTATAACCCAACAACCGATAAATGGACAGTTGTGTCATCGTGTATGAGCACAGGG
AGAAGTTATGCAGGGGTCACAGTTATTGATAAACCATTA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL2
Symbol Nomenclature : KLHL2
SYNONYM : ABP-KELCH; MAV; MAYVEN
Designation : actin-binding protein Mayven|kelch|kelch-like 2, Mayven|kelch-like protein 2
Full Nomenclature : kelch-like family member 2
GENEID : 11275
GenBank Accession : DQ895588
HGNC : 6353
MIM : 605774
Vega : OTTHUMG00000161294
Target GenBank: DQ895588

 

Reference Sequence Alignment

  

HsCD00514064        ------------ATGGAGACGCCGCCGCTGCCTCCCGCATGCACAAAGCAGGGTCATCAG
NM_001161521.1      ATGGTATGGTTGGAAGCACGGCCCCAGATTCTCTTTGTATGCACAAAGCAGGGTCATCAG
NM_007246.3         ------------ATGGAGACGCCGCCGCTGCCTCCCGCATGCACAAAGCAGGGTCATCAG
                                .:.*... *** *.*.* *     * **********************

HsCD00514064        AAGCCTCTCGATTCAAAAGATGATAATACCGAAAAACACTGCCCAGTGACAGTGAATCCT
NM_001161521.1      AAGCCTCTCGATTCAAAAGATGATAATACCGAAAAACACTGCCCAGTGACAGTGAATCCT
NM_007246.3         AAGCCTCTCGATTCAAAAGATGATAATACCGAAAAACACTGCCCAGTGACAGTGAATCCT
                    ************************************************************

HsCD00514064        TGGCATATGAAGAAAGCTTTCAAAGTCATGAACGAATTAAGAAGTCAAAATTTGCTGTGC
NM_001161521.1      TGGCATATGAAGAAAGCTTTCAAAGTCATGAACGAATTAAGAAGTCAAAATTTGCTGTGC
NM_007246.3         TGGCATATGAAGAAAGCTTTCAAAGTCATGAACGAATTAAGAAGTCAAAATTTGCTGTGC
                    ************************************************************

HsCD00514064        GATGTCACAATTGTGGCAGAAGACATGGAAATTTCTGCTCATAGAGTGGTGCTGGCCGCC
NM_001161521.1      GATGTCACAATTGTGGCAGAAGACATGGAAATTTCTGCTCATAGAGTGGTGCTGGCCGCC
NM_007246.3         GATGTCACAATTGTGGCAGAAGACATGGAAATTTCTGCTCATAGAGTGGTGCTGGCCGCC
                    ************************************************************

HsCD00514064        TGTAGTCCTTATTTTCATGCCATGTTTACAGGTGAGATGAGTGAGAGCCGAGCAAAGAGA
NM_001161521.1      TGTAGTCCTTATTTTCATGCCATGTTTACAGGTGAGATGAGTGAGAGCCGAGCAAAGAGA
NM_007246.3         TGTAGTCCTTATTTTCATGCCATGTTTACAGGTGAGATGAGTGAGAGCCGAGCAAAGAGA
                    ************************************************************

HsCD00514064        GTTAGAATAAAAGAGGTAGATGGCTGGACCCTGAGGATGCTAATTGATTATGTTTACACT
NM_001161521.1      GTTAGAATAAAAGAGGTAGATGGCTGGACCCTGAGGATGCTAATTGATTATGTTTACACT
NM_007246.3         GTTAGAATAAAAGAGGTAGATGGCTGGACCCTGAGGATGCTAATTGATTATGTTTACACT
                    ************************************************************

HsCD00514064        GCAGAAATTCAGGTTACAGAAGAAAATGTACAGGTACTTCTCCCAGCAGCTGGTCTCTTA
NM_001161521.1      GCAGAAATTCAGGTTACAGAAGAAAATGTACAGGTACTTCTCCCAGCAGCTGGTCTCTTA
NM_007246.3         GCAGAAATTCAGGTTACAGAAGAAAATGTACAGGTACTTCTCCCAGCAGCTGGTCTCTTA
                    ************************************************************

HsCD00514064        CAGTTACAGGATGTGAAGAAGACTTGTTGTGAATTTTTGGAATCCCAGCTTCACCCTGTC
NM_001161521.1      CAGTTACAGGATGTGAAGAAGACTTGTTGTGAATTTTTGGAATCCCAGCTTCACCCTGTC
NM_007246.3         CAGTTACAGGATGTGAAGAAGACTTGTTGTGAATTTTTGGAATCCCAGCTTCACCCTGTC
                    ************************************************************

HsCD00514064        AACTGCTTAGGAATCCGGGCTTTTGCTGATATGCATGCATGTACCGACCTTCTGAACAAG
NM_001161521.1      AACTGCTTAGGAATCCGGGCTTTTGCTGATATGCATGCATGTACCGACCTTCTGAACAAG
NM_007246.3         AACTGCTTAGGAATCCGGGCTTTTGCTGATATGCATGCATGTACCGACCTTCTGAACAAG
                    ************************************************************

HsCD00514064        GCCAACACCTATGCAGAGCAACATTTTGCAGATGTTGTACTTAGTGAAGAATTTCTCAAT
NM_001161521.1      GCCAACACCTATGCAGAGCAACATTTTGCAGATGTTGTACTTAGTGAAGAATTTCTCAAT
NM_007246.3         GCCAACACCTATGCAGAGCAACATTTTGCAGATGTTGTACTTAGTGAAGAATTTCTCAAT
                    ************************************************************

HsCD00514064        CTTGGCATCGAACAAGTGTGCAGCTTAATCTCAAGTGACAAACTTACCATTTCTTCAGAA
NM_001161521.1      CTTGGCATCGAACAAGTGTGCAGCTTAATCTCAAGTGACAAACTTACCATTTCTTCAGAA
NM_007246.3         CTTGGCATCGAACAAGTGTGCAGCTTAATCTCAAGTGACAAACTTACCATTTCTTCAGAA
                    ************************************************************

HsCD00514064        GAGAAGGTATTTGAAGCAGTAATAGCATGGGTGAACCATGACAAGGATGTGAGGCAAGAG
NM_001161521.1      GAGAAGGTATTTGAAGCAGTAATAGCATGGGTGAACCATGACAAGGATGTGAGGCAAGAG
NM_007246.3         GAGAAGGTATTTGAAGCAGTAATAGCATGGGTGAACCATGACAAGGATGTGAGGCAAGAG
                    ************************************************************

HsCD00514064        TTTATGGCCCGACTGATGGAACATGTACGGTTACCTTTGCTTCCTCGGGAATATTTAGTT
NM_001161521.1      TTTATGGCCCGACTGATGGAACATGTACGGTTACCTTTGCTTCCTCGGGAATATTTAGTT
NM_007246.3         TTTATGGCCCGACTGATGGAACATGTACGGTTACCTTTGCTTCCTCGGGAATATTTAGTT
                    ************************************************************

HsCD00514064        CAGAGGGTTGAAGAGGAAGCATTGGTCAAGAATAGCAGTGCTTGCAAAGATTACCTCATT
NM_001161521.1      CAGAGGGTTGAAGAGGAAGCATTGGTCAAGAATAGCAGTGCTTGCAAAGATTACCTCATT
NM_007246.3         CAGAGGGTTGAAGAGGAAGCATTGGTCAAGAATAGCAGTGCTTGCAAAGATTACCTCATT
                    ************************************************************

HsCD00514064        GAAGCAATGAAGTACCATTTGCTGCCAACAGAGCAGCGTATATTAATGAAGAGTGTCCGG
NM_001161521.1      GAAGCAATGAAGTACCATTTGCTGCCAACAGAGCAGCGTATATTAATGAAGAGTGTCCGG
NM_007246.3         GAAGCAATGAAGTACCATTTGCTGCCAACAGAGCAGCGTATATTAATGAAGAGTGTCCGG
                    ************************************************************

HsCD00514064        ACCCGGCTGAGGACACCCATGAACCTTCCCAAATTGATGGTGGTGGTTGGGGGCCAAGCA
NM_001161521.1      ACCCGGCTGAGGACACCCATGAACCTTCCCAAATTGATGGTGGTGGTTGGGGGCCAAGCA
NM_007246.3         ACCCGGCTGAGGACACCCATGAACCTTCCCAAATTGATGGTGGTGGTTGGGGGCCAAGCA
                    ************************************************************

HsCD00514064        CCAAAGGCTATCCGGAGTGTGGAATGCTATGACTTTAAAGAAGAAAGGTGGCACCAAGTA
NM_001161521.1      CCAAAGGCTATCCGGAGTGTGGAATGCTATGACTTTAAAGAAGAAAGGTGGCACCAAGTA
NM_007246.3         CCAAAGGCTATCCGGAGTGTGGAATGCTATGACTTTAAAGAAGAAAGGTGGCACCAAGTA
                    ************************************************************

HsCD00514064        GCAGAGTTGCCTTCCGGGAGGTGCAGGGCAGGCATGGTCTACATGGCTGGACTTGTTTTT
NM_001161521.1      GCAGAGTTGCCTTCCAGGAGGTGCAGGGCAGGCATGGTCTACATGGCTGGACTTGTTTTT
NM_007246.3         GCAGAGTTGCCTTCCAGGAGGTGCAGGGCAGGCATGGTCTACATGGCTGGACTTGTTTTT
                    ***************.********************************************

HsCD00514064        GCTGTTGGTGGCTTTAATGGCTCATTAAGAGTTCGCACTGTAGATTCCTACGACCCTGTG
NM_001161521.1      GCTGTTGGTGGCTTTAATGGCTCATTAAGAGTTCGCACTGTAGATTCCTACGACCCTGTG
NM_007246.3         GCTGTTGGTGGCTTTAATGGCTCATTAAGAGTTCGCACTGTAGATTCCTACGACCCTGTG
                    ************************************************************

HsCD00514064        AAGGACCAGTGGACCAGCGTTGCTAACATGAGAGACCGGAGAAGCACTTTGGGAGCTGCT
NM_001161521.1      AAGGACCAGTGGACCAGCGTTGCTAACATGAGAGACCGGAGAAGCACTTTGGGAGCTGCT
NM_007246.3         AAGGACCAGTGGACCAGCGTTGCTAACATGAGAGACCGGAGAAGCACTTTGGGAGCTGCT
                    ************************************************************

HsCD00514064        GTGTTAAATGGATTATTATACGCTGTGGGAGGCTTTGATGGGAGTACAGGTTTGTCATCT
NM_001161521.1      GTGTTAAATGGATTATTATACGCTGTGGGAGGCTTTGATGGGAGTACAGGTTTGTCATCT
NM_007246.3         GTGTTAAATGGATTATTATACGCTGTGGGAGGCTTTGATGGGAGTACAGGTTTGTCATCT
                    ************************************************************

HsCD00514064        GTGGAAGCATACAACATAAAGTCTAATGAGTGGTTTCATGTAGCTCCCATGAATACAAGG
NM_001161521.1      GTGGAAGCATACAACATAAAGTCTAATGAGTGGTTTCATGTAGCTCCCATGAATACAAGG
NM_007246.3         GTGGAAGCATACAACATAAAGTCTAATGAGTGGTTTCATGTAGCTCCCATGAATACAAGG
                    ************************************************************

HsCD00514064        AGGAGCAGTGTTGGTGTGGGTGTTGTTGGAGGTTTGCTCTATGCTGTAGGAGGTTATGAT
NM_001161521.1      AGGAGCAGTGTTGGTGTGGGTGTTGTTGGAGGTTTGCTCTATGCTGTAGGAGGTTATGAT
NM_007246.3         AGGAGCAGTGTTGGTGTGGGTGTTGTTGGAGGTTTGCTCTATGCTGTAGGAGGTTATGAT
                    ************************************************************

HsCD00514064        GGAGCATCACGTCAGTGTCTTAGCACAGTAGAATGCTATAATGCTACAACAAATGAGTGG
NM_001161521.1      GGAGCATCACGTCAGTGTCTTAGCACAGTAGAATGCTATAATGCTACAACAAATGAGTGG
NM_007246.3         GGAGCATCACGTCAGTGTCTTAGCACAGTAGAATGCTATAATGCTACAACAAATGAGTGG
                    ************************************************************

HsCD00514064        ACCTATATAGCAGAAATGAGCACCAGGCGGAGTGGAGCAGGTGTTGGTGTGTTAAACAAT
NM_001161521.1      ACCTATATAGCAGAAATGAGCACCAGGCGGAGTGGAGCAGGTGTTGGTGTGTTAAACAAT
NM_007246.3         ACCTATATAGCAGAAATGAGCACCAGGCGGAGTGGAGCAGGTGTTGGTGTGTTAAACAAT
                    ************************************************************

HsCD00514064        TTATTGTATGCTGTAGGAGGTCATGATGGCCCTTTAGTACGAAAAAGTGTTGAAGTATAT
NM_001161521.1      TTATTGTATGCTGTAGGAGGTCATGATGGCCCTTTAGTACGAAAAAGTGTTGAAGTATAT
NM_007246.3         TTATTGTATGCTGTAGGAGGTCATGATGGCCCTTTAGTACGAAAAAGTGTTGAAGTATAT
                    ************************************************************

HsCD00514064        GATCCCACCACTAACGCATGGAGACAGGTTGCAGATATGAACATGTGCAGAAGAAATGCA
NM_001161521.1      GATCCCACCACTAACGCATGGAGACAGGTTGCAGATATGAACATGTGCAGAAGAAATGCA
NM_007246.3         GATCCCACCACTAACGCATGGAGACAGGTTGCAGATATGAACATGTGCAGAAGAAATGCA
                    ************************************************************

HsCD00514064        GGAGTTTGTGCAGTTAATGGTCTGTTATATGTTGTTGGAGGGGATGATGGTTCCTGTAAC
NM_001161521.1      GGAGTTTGTGCAGTTAATGGTCTGTTATATGTTGTTGGAGGGGATGATGGTTCCTGTAAC
NM_007246.3         GGAGTTTGTGCAGTTAATGGTCTGTTATATGTTGTTGGAGGGGATGATGGTTCCTGTAAC
                    ************************************************************

HsCD00514064        TTGGCGTCAGTAGAATATTATAACCCAACAACCGATAAATGGACAGTTGTGTCATCGTGT
NM_001161521.1      TTGGCGTCAGTAGAATATTATAACCCAACAACCGATAAATGGACAGTTGTGTCATCGTGT
NM_007246.3         TTGGCGTCAGTAGAATATTATAACCCAACAACCGATAAATGGACAGTTGTGTCATCGTGT
                    ************************************************************

HsCD00514064        ATGAGCACAGGGAGAAGTTATGCAGGGGTCACAGTTATTGATAAACCATTA---
NM_001161521.1      ATGAGCACAGGGAGAAGTTATGCAGGGGTCACAGTTATTGATAAACCATTATGA
NM_007246.3         ATGAGCACAGGGAGAAGTTATGCAGGGGTCACAGTTATTGATAAACCATTATGA
                    ***************************************************   

Protein

SMART domain : Kelch :
SMART domain : BACK : BTB And C-terminal Kelch
SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
HPRD : 09311

Clone

Cloning Information : 607044
Original Clone ID : ccsbBroadEn_07792

Organism


TAX_ID : 9606
Species Specific ID: 11275

Genome

Chromosome : 4
Map Location : 4q21.2
Ensembl : ENSG00000109466

Reagents

Labome : KLHL2-antibody