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SRBD1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  SRBD1
Gene Name:  S1 RNA binding domain 1
Sequence             
Original Clone ID: ccsbBroadEn_12167
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC032538.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00514215

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1861nts         Open reading frame : 1 to 1861
Reference Sequence Alignment

ATGATTGCTAAAGACAAAGACACGCTTGACTTCATTCGGAACTTGTGCCAGAAGAGACAT
GTTTGTATCCAGTCATCTCTGGCAAAAGTATCCTCAAAAAAGGTAAATGAGAAAGATGTT
GATAAGTTTCTGCTCTACCAGCATTTTTCCTGCAACATAAGAAACATTCACCATCATCAG
ATTCTGGCAATTAACCGTGGAGAAAATTTGAAGGTACTGACGGTTAAGGTCAATATTTCT
GATGGAGTGAAGGATGAATTCTGTAGGTGGTGCATCCAAAACAGGTGGAGACCACGTAGC
TTTGCAAGGCCAGAGTTAATGAAGATCTTATATAATTCACTGAATGATTCCTTTAAACGC
CTTATTTATCCTCTTCTCTGTAGAGAATTCAGAGCCAAACTAACATCAGATGCAGAGAAG
GAATCAGTAATGATGTTTGGACGGAACCTTCGTCAGCTCCTTTTAACAAGCCCTGTTCCA
GGGCGCACCTTAATGGGAGTGGATCCTGGTTATAAACATGGTTGCAAATTAGCTATAATT
TCTCCTACTAGTCAGATACTTCATACTGATGTGGTTTACTTGCATTGTGGACAAGGCTTC
CGAGAGGCGGAGAAAATAAAGACACTTTTGCTGAATTTCAACTGCAGCACAGTAGTGATT
GGAAATGGAACTGCCTGCAGGGAAACAGAAGCTTACTTTGCTGACCTGATAATGAAGAAT
TATTTTGCACCACTGGATGTTGTTTACTGTATCGTCAGTGAAGCAGGAGCATCAATCTAC
AGTGTCAGCCCTGAAGCTAACAAAGAGATGCCAGGGCTGGACCCTAATTTGAGAAGTGCA
GTTTCCATAGCAAGGCGTGTACAAGATCCATTAGCTGAGCTAGTGAAAATTGAGCCAAAG
CACATTGGAGTTGGAATGTATCAGCATGACGTATCCCAGACTTTACTCAAGGCAACACTG
GACAGTGTTGTAGAAGAATGTGTCAGCTTTGTGGGAGTGGATATTAACATCTGTTCAGAA
GTTTTGTTAAGGCATATTGCAGGACTCAATGCCAACAGGGCCAAAAATATTATTGAATGG
CGAGAGAAAAATGGACCCTTTATCAACCGAGAACAGCTGAAGAAAGTGAAAGGGCTGGGC
CCAAAATCCTTCCAACAGTGTGCTGGCTTCATCAGAATCAACCAGGATTATATCCGAACG
TTTTGCAGTCAGCAAACTGAAACTTCAGGCCAAATTCAAGGAGTTGCTGTGACATCTTCA
GCAGACGTTGAGGTCACAAATGAGAAGCAGGGCAAAAAGAAGAGCAAAACTGCAGTGAAT
GTTTTACTGAAGCCAAATCCTTTGGACCAAACTTGTATTCATCCAGAATCATATGACATA
GCAATGAGGTTTTTGTCATCCATTGGAGGGACACTGTATGAGGTTGGAAAGCCTGAAATG
CAACAAAAAATAAATTCATTCCTTGAAAAGGAAGGAATGGAGAAAATTGCAGAAAGATTG
CAAACAACAGTACACACCTTACAGGTCATCATAGATGGTCTCAGCCAGCCTGAAAGCTTT
GACTTTCGAACAGATTTTGATAAACCTGATTTCAAGAGAAGCATAGTATGCCTGGAAGAT
CTGCAGATTGGGACAGTTCTTACAGGCAAAGTTGAGAATGCCACTCTCTTTGGAATTTTT
GTGGATATAGGAGTGGGGAAATCTGGGCTGATTCCCATACGAAATGTAACAGAAGCAAAA
CTTTCAAAAACAAAGAAGAGAAGAAGCCTTGGACTGGGCCCCGGAGAAAGAGTGGAAGTC
CAAGTACTCAACATTGACATCCCCCGATCTAGGATTACTCTGGACCTCATTCGGGTGTTA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : SRBD1
Symbol Nomenclature : SRBD1
Designation : H_NH0576F01.1|S1 RNA-binding domain-containing protein 1|WUGSC:H_NH0576F01.1
Full Nomenclature : S1 RNA binding domain 1
GENEID : 55133
GenBank Accession : JF432253
HGNC : 25521
Vega : OTTHUMG00000128814
Target GenBank: JF432253

 

Reference Sequence Alignment

  

HsCD00514215      ------------------------------------------------------------
NM_018079.4       ATGTCATCATTGCCAAGAAGAGCGAAAGTACAGGTCCAGGATGTGGTACTGAAAGATGAA
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       TTTTCTTCATTCTCTGAGTTATCATCTGCCTCTGAAGAAGATGACAAGGAAGATAGTGCC
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       TGGGAGCCCCAAAAGAAAGTTCCCAGAAGCCGTAAACAGCCCCCTCCCAAGGAATCCAAA
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       CCAAAGAGGATGCCTCGGGTGAAGAAGAATGCCCCACAGATCAGTGATGGCTCAGAAGTC
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       GTTGTTGTTAAGGAGGAGCTGAATAGCTCTGTGGCTATTGCTGATACTGCTTTAGAAGAC
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       AGAAAAAATAAATTGGATACTGTACAGACTCTGAAAACAGCCAAGACAAAACAGAAATGT
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       GCAGCGCAGCCACATACAGTTCGAAGGACTAAAAAGCTGAAAGTTGAAGAAGAAACCAGC
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       AAAGCCAGCAACTTAGAGGGTGAGAGTAATAGTTCAGAGACACCATCCACAAGCACTGTG
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       TGGGGAGGTACATGCAAGAAGGAAGAGAATGACGATGACTTTACATTTGGTCAGTCCGCT
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       TTAAAGAAAATCAAGACTGAGACATATCCTCAGGGGCAGCCTGTCAAGTTTCCAGCAAAT
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       GCCAATAGTACTAAAGAGGAGGTGGAAATGAATTGGGACATGGTACAGGTTTTATCTGAG
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       AGAACTAATATTGAACCTTGGGTTTGTGCCAACATCATTCGTCTCTTTAATGATGATAAC
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       ACAATTCCCTTCATTATACGTTATAGAAAAGAGCTCATTAATAACCTTGATGCTGATTCC
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       TTGAGAGAAGTTCAGCAAACCCTAGAAGAGCTACGGGCTGTTGCAAAGAAAGTTCATAGT
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       ACAATCCAGAAAATTAAGAAGGAAGGGAAGATGTCTGAGTGCTTGTTAAAAGCCATGCTG
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       AATTGTAAAACTTTTGAAGAACTAGAACACGTGTCTGCTCCATATAAAACTGGAAGCAAA
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       GGGACTAAAGCCCAGAGAGCAAGACAGTTGGGCTTAGAAGGAGCAGCCAGGGCACTGCTT
                                                                              

HsCD00514215      ------------------------------------------------------------
NM_018079.4       GAGAAACCAGGGGAGCTCAGTCTGCTATCGTACATTAGGCCTGACGTTAAAGGGCTTTCA
                                                                              

HsCD00514215      ---------------------------------------------ATGATTGCTAAAGAC
NM_018079.4       ACGCTTCAGGATATTGAAATAGGAGTGCAGCATATTTTAGCAGATATGATTGCTAAAGAC
                                                               ***************

HsCD00514215      AAAGACACGCTTGACTTCATTCGGAACTTGTGCCAGAAGAGACATGTTTGTATCCAGTCA
NM_018079.4       AAAGACACGCTTGACTTCATTCGGAACTTGTGCCAGAAGAGACATGTTTGTATCCAGTCA
                  ************************************************************

HsCD00514215      TCTCTGGCAAAAGTATCCTCAAAAAAGGTAAATGAGAAAGATGTTGATAAGTTTCTGCTC
NM_018079.4       TCTCTGGCAAAAGTATCCTCAAAAAAGGTAAATGAGAAAGATGTTGATAAGTTTCTGCTC
                  ************************************************************

HsCD00514215      TACCAGCATTTTTCCTGCAACATAAGAAACATTCACCATCATCAGATTCTGGCAATTAAC
NM_018079.4       TACCAGCATTTTTCCTGCAACATAAGAAACATTCACCATCATCAGATTCTGGCAATTAAC
                  ************************************************************

HsCD00514215      CGTGGAGAAAATTTGAAGGTACTGACGGTTAAGGTCAATATTTCTGATGGAGTGAAGGAT
NM_018079.4       CGTGGAGAAAATTTGAAGGTACTGACGGTTAAGGTCAATATTTCTGATGGAGTGAAGGAT
                  ************************************************************

HsCD00514215      GAATTCTGTAGGTGGTGCATCCAAAACAGGTGGAGACCACGTAGCTTTGCAAGGCCAGAG
NM_018079.4       GAATTCTGTAGGTGGTGCATCCAAAACAGGTGGAGACCACGTAGCTTTGCAAGGCCAGAG
                  ************************************************************

HsCD00514215      TTAATGAAGATCTTATATAATTCACTGAATGATTCCTTTAAACGCCTTATTTATCCTCTT
NM_018079.4       TTAATGAAGATCTTATATAATTCACTGAATGATTCCTTTAAACGCCTTATTTATCCTCTT
                  ************************************************************

HsCD00514215      CTCTGTAGAGAATTCAGAGCCAAACTAACATCAGATGCAGAGAAGGAATCAGTAATGATG
NM_018079.4       CTCTGTAGAGAATTCAGAGCCAAACTAACATCAGATGCAGAGAAGGAATCAGTAATGATG
                  ************************************************************

HsCD00514215      TTTGGACGGAACCTTCGTCAGCTCCTTTTAACAAGCCCTGTTCCAGGGCGCACCTTAATG
NM_018079.4       TTTGGACGGAACCTTCGTCAGCTCCTTTTAACAAGCCCTGTTCCAGGGCGCACCTTAATG
                  ************************************************************

HsCD00514215      GGAGTGGATCCTGGTTATAAACATGGTTGCAAATTAGCTATAATTTCTCCTACTAGTCAG
NM_018079.4       GGAGTGGATCCTGGTTATAAACATGGTTGCAAATTAGCTATAATTTCTCCTACTAGTCAG
                  ************************************************************

HsCD00514215      ATACTTCATACTGATGTGGTTTACTTGCATTGTGGACAAGGCTTCCGAGAGGCGGAGAAA
NM_018079.4       ATACTTCATACTGATGTGGTTTACTTGCATTGTGGACAAGGCTTCCGAGAGGCGGAGAAA
                  ************************************************************

HsCD00514215      ATAAAGACACTTTTGCTGAATTTCAACTGCAGCACAGTAGTGATTGGAAATGGAACTGCC
NM_018079.4       ATAAAGACACTTTTGCTGAATTTCAACTGCAGCACAGTAGTGATTGGAAATGGAACTGCC
                  ************************************************************

HsCD00514215      TGCAGGGAAACAGAAGCTTACTTTGCTGACCTGATAATGAAGAATTATTTTGCACCACTG
NM_018079.4       TGCAGGGAAACAGAAGCTTACTTTGCTGACCTGATAATGAAGAATTATTTTGCACCACTG
                  ************************************************************

HsCD00514215      GATGTTGTTTACTGTATCGTCAGTGAAGCAGGAGCATCAATCTACAGTGTCAGCCCTGAA
NM_018079.4       GATGTTGTTTACTGTATCGTCAGTGAAGCAGGAGCATCAATCTACAGTGTCAGCCCTGAA
                  ************************************************************

HsCD00514215      GCTAACAAAGAGATGCCAGGGCTGGACCCTAATTTGAGAAGTGCAGTTTCCATAGCAAGG
NM_018079.4       GCTAACAAAGAGATGCCAGGGCTGGACCCTAATTTGAGAAGTGCAGTTTCCATAGCAAGG
                  ************************************************************

HsCD00514215      CGTGTACAAGATCCATTAGCTGAGCTAGTGAAAATTGAGCCAAAGCACATTGGAGTTGGA
NM_018079.4       CGTGTACAAGATCCATTAGCTGAGCTAGTGAAAATTGAGCCAAAGCACATTGGAGTTGGA
                  ************************************************************

HsCD00514215      ATGTATCAGCATGACGTATCCCAGACTTTACTCAAGGCAACACTGGACAGTGTTGTAGAA
NM_018079.4       ATGTATCAGCATGACGTATCCCAGACTTTACTCAAGGCAACACTGGACAGTGTTGTAGAA
                  ************************************************************

HsCD00514215      GAATGTGTCAGCTTTGTGGGAGTGGATATTAACATCTGTTCAGAAGTTTTGTTAAGGCAT
NM_018079.4       GAATGTGTCAGCTTTGTGGGAGTGGATATTAACATCTGTTCAGAAGTTTTGTTAAGGCAT
                  ************************************************************

HsCD00514215      ATTGCAGGACTCAATGCCAACAGGGCCAAAAATATTATTGAATGGCGAGAGAAAAATGGA
NM_018079.4       ATTGCAGGACTCAATGCCAACAGGGCCAAAAATATTATTGAATGGCGAGAGAAAAATGGA
                  ************************************************************

HsCD00514215      CCCTTTATCAACCGAGAACAGCTGAAGAAAGTGAAAGGGCTGGGCCCAAAATCCTTCCAA
NM_018079.4       CCCTTTATCAACCGAGAACAGCTGAAGAAAGTGAAAGGGCTGGGCCCAAAATCCTTCCAA
                  ************************************************************

HsCD00514215      CAGTGTGCTGGCTTCATCAGAATCAACCAGGATTATATCCGAACGTTTTGCAGTCAGCAA
NM_018079.4       CAGTGTGCTGGCTTCATCAGAATCAACCAGGATTATATCCGAACGTTTTGCAGTCAGCAA
                  ************************************************************

HsCD00514215      ACTGAAACTTCAGGCCAAATTCAAGGAGTTGCTGTGACATCTTCAGCAGACGTTGAGGTC
NM_018079.4       ACTGAAACTTCAGGCCAAATTCAAGGAGTTGCTGTGACATCTTCAGCAGACGTTGAGGTC
                  ************************************************************

HsCD00514215      ACAAATGAGAAGCAGGGCAAAAAGAAGAGCAAAACTGCAGTGAATGTTTTACTGAAGCCA
NM_018079.4       ACAAATGAGAAGCAGGGCAAAAAGAAGAGCAAAACTGCAGTGAATGTTTTACTGAAGCCA
                  ************************************************************

HsCD00514215      AATCCTTTGGACCAAACTTGTATTCATCCAGAATCATATGACATAGCAATGAGGTTTTTG
NM_018079.4       AATCCTTTGGACCAAACTTGTATTCATCCAGAATCATATGACATAGCAATGAGGTTTTTG
                  ************************************************************

HsCD00514215      TCATCCATTGGAGGGACACTGTATGAGGTTGGAAAGCCTGAAATGCAACAAAAAATAAAT
NM_018079.4       TCATCCATTGGAGGGACACTGTATGAGGTTGGAAAGCCTGAAATGCAACAAAAAATAAAT
                  ************************************************************

HsCD00514215      TCATTCCTTGAAAAGGAAGGAATGGAGAAAATTGCAGAAAGATTGCAAACAACAGTACAC
NM_018079.4       TCATTCCTTGAAAAGGAAGGAATGGAGAAAATTGCAGAAAGATTGCAAACAACAGTACAC
                  ************************************************************

HsCD00514215      ACCTTACAGGTCATCATAGATGGTCTCAGCCAGCCTGAAAGCTTTGACTTTCGAACAGAT
NM_018079.4       ACCTTACAGGTCATCATAGATGGTCTCAGCCAGCCTGAAAGCTTTGACTTTCGAACAGAT
                  ************************************************************

HsCD00514215      TTTGATAAACCTGATTTCAAGAGAAGCATAGTATGCCTGGAAGATCTGCAGATTGGGACA
NM_018079.4       TTTGATAAACCTGATTTCAAGAGAAGCATAGTATGCCTGGAAGATCTGCAGATTGGGACA
                  ************************************************************

HsCD00514215      GTTCTTACAGGCAAAGTTGAGAATGCCACTCTCTTTGGAATTTTTGTGGATATAGGAGTG
NM_018079.4       GTTCTTACAGGCAAAGTTGAGAATGCCACTCTCTTTGGAATTTTTGTGGATATAGGAGTG
                  ************************************************************

HsCD00514215      GGGAAATCTGGGCTGATTCCCATACGAAATGTAACAGAAGCAAAACTTTCAAAAACAAAG
NM_018079.4       GGGAAATCTGGGCTGATTCCCATACGAAATGTAACAGAAGCAAAACTTTCAAAAACAAAG
                  ************************************************************

HsCD00514215      AAGAGAAGAAGCCTTGGACTGGGCCCCGGAGAAAGAGTGGAAGTCCAAGTACTCAACATT
NM_018079.4       AAGAGAAGAAGCCTTGGACTGGGCCCCGGAGAAAGAGTGGAAGTCCAAGTACTCAACATT
                  ************************************************************

HsCD00514215      GACATCCCCCGATCTAGGATTACTCTGGACCTCATTCGGGTGTTA---
NM_018079.4       GACATCCCCCGATCTAGGATTACTCTGGACCTCATTCGGGTGTTATGA
                  *********************************************   

Clone

Cloning Information : 606913
Original Clone ID : ccsbBroadEn_12167

Organism


TAX_ID : 9606
Species Specific ID: 55133

Genome

Chromosome : 2
Map Location : 2p21
Ensembl : ENSG00000068784

Reagents

Labome : SRBD1-antibody