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CAPN5 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN5
Gene Name:  calpain 5
Sequence             
Original Clone ID: ccsbBroadEn_00187
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC018123.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00514241

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1921nts         Open reading frame : 1 to 1921
Reference Sequence Alignment

ATGTTCTCGTGTGTGAAGCCCTATGAGGACCAGAACTACTCAGCCCTGAGGCGGGACTGC
CGGCGCAGGAAGGTGCTCTTCGAGGACCCCCTCTTCCCCGCCACTGACGACTCACTCTAC
TATAAGGGCACGCCGGGGCCCGCCGTCAGGTGGAAGCGACCCAAGGGCATCTGCGAGGAC
CCCCGCCTCTTTGTGGATGGCATCAGCTCCCACGACCTGCACCAGGGCCAGGTGGGCAAC
TGCTGGTTTGTGGCAGCCTGCTCGTCACTTGCCTCCCGGGAGTCGCTGTGGCAAAAGGTC
ATCCCAGACTGGAAGGAGCAGGAATGGGACCCCGAAAAGCCCAACGCCTACGCGGGCATC
TTCCACTTCCACTTCTGGCGCTTCGGGGAATGGGTGGACGTGGTCATCGATGACCGGCTG
CCCACAGTCAACAACCAGCTCATCTACTGCCACTCCAACTCCCGCAATGAGTTTTGGTGC
GCCCTAGTGGAGAAGGCCTATGCCAAACTGGCAGGCTGTTACCAGGCCCTGGATGGAGGC
AACACAGCAGACGCACTGGTGGACTTCACGGGTGGTGTTTCTGAGCCCATCGACCTGACC
GAGGGTGACTTTGCCAACGATGAGACTAAGAGGAACCAGCTCTTTGAGCGCATGTTAAAG
GTGCACAGCCGGGGCGGCCTCATCAGTGCCTCCATCAAGGCAGTGACAGCAGCTGACATG
GAGGCCCGCCTGGCGTGCGGCCTGGTAAAGGGCCACGCATACGCCGTCACTGATGTGCGC
AAGGTGCGCCTGGGCCACGGCCTACTGGCCTTCTTCAAGTCAGAGAAGTTGGACATGATC
CGCCTGCGCAACCCCTGGGGCGAGCGGGAGTGGAACGGGCCCTGGAGTGACACCTCGGAG
GAGTGGCAGAAAGTGAGCAAGAGTGAGCGGGAGAAGATGGGTGTGACCGTGCAGGACGAC
GGTGAGTTCTGGATGACCTTCGAGGACGTGTGCCGGTACTTCACGGACATCATCAAGTGC
CGCGTGATCAACACATCCCACCTGAGCATCCACAAGACGTGGGAGGAGGCCCGGCTGCAT
GGCGCCTGGACGCTGCATGAGGACCCGCGACAGAACCGCGGTGGCGGCTGCATCAACCAC
AAGGACACCTTCTTCCAGAACCCACAGTACATCTTCGAAGTCAAGAAGCCAGAAGATGAA
GTCCTGATCTGCATCCAGCAGCGGCCAAAGCGGTCTACGCGCCGGGAGGGCAAGGGTGAG
AACCTGGCCATTGGCTTTGACATCTACAAGGTGGAGGAGAACCGCCAGTACCGCATGCAC
AGCCTGCAGCACAAGGCCGCCAGCTCCATCTACATCAACTCACGCAGCGTCTTCCTGCGC
ACCGACCAGCCCGAGGGCCGCTATGTCATCATCCCCACAACCTTCGAGCCAGGCCACACT
GGCGAGTTCCTGCTCCGAGTCTTCACTGATGTGCCCTCCAACTGCCGGGAGCTGCGCCTG
GATGAGCCCCCACACACCTGCTGGAGCTCCCTCTGTGGCTACCCCCAGCTGGTGACCCAG
GTACATGTCCTGGGAGCTGCTGGCCTCAAGGACTCCCCAACAGGGGCTAACTCTTATGTG
ATCATCAAGTGTGAGGGAGACAAAGTCCGCTCGGCTGTGCAGAAGGGCACCTCCACACCA
GAGTACAATGTGAAAGGCATCTTCTACCGCAAGAAGCTGAGCCAGCCCATCACTGTACAG
GTCTGGAACCACCGAGTGCTGAAGGATGAATTTCTGGGCCAGGTGCACCTAAAGGCTGAC
CCGGACAACCTCCAGGCCCTGCATACCCTCCACCTCCGGGACCGAAATAGCCGGCAGCCC
AGCAACCTGCCAGGCACTGTGGCCGTGCACATTCTCAGCAGCACCTCCCTCATGGCTGTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN5
Symbol Nomenclature : CAPN5
SYNONYM : ADNIV; HTRA3; VRNI; nCL-3
Designation : calpain htra-3|calpain-5|new calpain 3|vitreoretinopathy, neovascular inflammatory
Full Nomenclature : calpain 5
GENEID : 726
GenBank Accession : EU176587
HGNC : 1482
MIM : 602537
Vega : OTTHUMG00000165192
Target GenBank: EU176587

 

Reference Sequence Alignment

  

HsCD00514241      ATGTTCTCGTGTGTGAAGCCCTATGAGGACCAGAACTACTCAGCCCTGAGGCGGGACTGC
NM_004055.4       ATGTTCTCGTGTGTGAAGCCCTATGAGGACCAGAACTACTCAGCCCTGAGGCGGGACTGC
                  ************************************************************

HsCD00514241      CGGCGCAGGAAGGTGCTCTTCGAGGACCCCCTCTTCCCCGCCACTGACGACTCACTCTAC
NM_004055.4       CGGCGCAGGAAGGTGCTCTTCGAGGACCCCCTCTTCCCCGCCACTGACGACTCACTCTAC
                  ************************************************************

HsCD00514241      TATAAGGGCACGCCGGGGCCCGCCGTCAGGTGGAAGCGACCCAAGGGCATCTGCGAGGAC
NM_004055.4       TATAAGGGCACGCCGGGGCCCGCCGTCAGGTGGAAGCGACCCAAGGGCATCTGCGAGGAC
                  ************************************************************

HsCD00514241      CCCCGCCTCTTTGTGGATGGCATCAGCTCCCACGACCTGCACCAGGGCCAGGTGGGCAAC
NM_004055.4       CCCCGCCTCTTTGTGGATGGCATCAGCTCCCACGACCTGCACCAGGGCCAGGTGGGCAAC
                  ************************************************************

HsCD00514241      TGCTGGTTTGTGGCAGCCTGCTCGTCACTTGCCTCCCGGGAGTCGCTGTGGCAAAAGGTC
NM_004055.4       TGCTGGTTTGTGGCAGCCTGCTCGTCACTTGCCTCCCGGGAGTCGCTGTGGCAAAAGGTC
                  ************************************************************

HsCD00514241      ATCCCAGACTGGAAGGAGCAGGAATGGGACCCCGAAAAGCCCAACGCCTACGCGGGCATC
NM_004055.4       ATCCCAGACTGGAAGGAGCAGGAATGGGACCCCGAAAAGCCCAACGCCTACGCGGGCATC
                  ************************************************************

HsCD00514241      TTCCACTTCCACTTCTGGCGCTTCGGGGAATGGGTGGACGTGGTCATCGATGACCGGCTG
NM_004055.4       TTCCACTTCCACTTCTGGCGCTTCGGGGAATGGGTGGACGTGGTCATCGATGACCGGCTG
                  ************************************************************

HsCD00514241      CCCACAGTCAACAACCAGCTCATCTACTGCCACTCCAACTCCCGCAATGAGTTTTGGTGC
NM_004055.4       CCCACAGTCAACAACCAGCTCATCTACTGCCACTCCAACTCCCGCAATGAGTTTTGGTGC
                  ************************************************************

HsCD00514241      GCCCTAGTGGAGAAGGCCTATGCCAAACTGGCAGGCTGTTACCAGGCCCTGGATGGAGGC
NM_004055.4       GCCCTAGTGGAGAAGGCCTATGCCAAACTGGCAGGCTGTTACCAGGCCCTGGATGGAGGC
                  ************************************************************

HsCD00514241      AACACAGCAGACGCACTGGTGGACTTCACGGGTGGTGTTTCTGAGCCCATCGACCTGACC
NM_004055.4       AACACAGCAGACGCACTGGTGGACTTCACGGGTGGTGTTTCTGAGCCCATCGACCTGACC
                  ************************************************************

HsCD00514241      GAGGGTGACTTTGCCAACGATGAGACTAAGAGGAACCAGCTCTTTGAGCGCATGTTAAAG
NM_004055.4       GAGGGTGACTTTGCCAACGATGAGACTAAGAGGAACCAGCTCTTTGAGCGCATGTTAAAG
                  ************************************************************

HsCD00514241      GTGCACAGCCGGGGCGGCCTCATCAGTGCCTCCATCAAGGCAGTGACAGCAGCTGACATG
NM_004055.4       GTGCACAGCCGGGGCGGCCTCATCAGTGCCTCCATCAAGGCAGTGACAGCAGCTGACATG
                  ************************************************************

HsCD00514241      GAGGCCCGCCTGGCGTGCGGCCTGGTAAAGGGCCACGCATACGCCGTCACTGATGTGCGC
NM_004055.4       GAGGCCCGCCTGGCGTGCGGCCTGGTAAAGGGCCACGCATACGCCGTCACTGATGTGCGC
                  ************************************************************

HsCD00514241      AAGGTGCGCCTGGGCCACGGCCTACTGGCCTTCTTCAAGTCAGAGAAGTTGGACATGATC
NM_004055.4       AAGGTGCGCCTGGGCCACGGCCTACTGGCCTTCTTCAAGTCAGAGAAGTTGGACATGATC
                  ************************************************************

HsCD00514241      CGCCTGCGCAACCCCTGGGGCGAGCGGGAGTGGAACGGGCCCTGGAGTGACACCTCGGAG
NM_004055.4       CGCCTGCGCAACCCCTGGGGCGAGCGGGAGTGGAACGGGCCCTGGAGTGACACCTCGGAG
                  ************************************************************

HsCD00514241      GAGTGGCAGAAAGTGAGCAAGAGTGAGCGGGAGAAGATGGGTGTGACCGTGCAGGACGAC
NM_004055.4       GAGTGGCAGAAAGTGAGCAAGAGTGAGCGGGAGAAGATGGGTGTGACCGTGCAGGACGAC
                  ************************************************************

HsCD00514241      GGTGAGTTCTGGATGACCTTCGAGGACGTGTGCCGGTACTTCACGGACATCATCAAGTGC
NM_004055.4       GGTGAGTTCTGGATGACCTTCGAGGACGTGTGCCGGTACTTCACGGACATCATCAAGTGC
                  ************************************************************

HsCD00514241      CGCGTGATCAACACATCCCACCTGAGCATCCACAAGACGTGGGAGGAGGCCCGGCTGCAT
NM_004055.4       CGCGTGATCAACACATCCCACCTGAGCATCCACAAGACGTGGGAGGAGGCCCGGCTGCAT
                  ************************************************************

HsCD00514241      GGCGCCTGGACGCTGCATGAGGACCCGCGACAGAACCGCGGTGGCGGCTGCATCAACCAC
NM_004055.4       GGCGCCTGGACGCTGCATGAGGACCCGCGACAGAACCGCGGTGGCGGCTGCATCAACCAC
                  ************************************************************

HsCD00514241      AAGGACACCTTCTTCCAGAACCCACAGTACATCTTCGAAGTCAAGAAGCCAGAAGATGAA
NM_004055.4       AAGGACACCTTCTTCCAGAACCCACAGTACATCTTCGAAGTCAAGAAGCCAGAAGATGAA
                  ************************************************************

HsCD00514241      GTCCTGATCTGCATCCAGCAGCGGCCAAAGCGGTCTACGCGCCGGGAGGGCAAGGGTGAG
NM_004055.4       GTCCTGATCTGCATCCAGCAGCGGCCAAAGCGGTCTACGCGCCGGGAGGGCAAGGGTGAG
                  ************************************************************

HsCD00514241      AACCTGGCCATTGGCTTTGACATCTACAAGGTGGAGGAGAACCGCCAGTACCGCATGCAC
NM_004055.4       AACCTGGCCATTGGCTTTGACATCTACAAGGTGGAGGAGAACCGCCAGTACCGCATGCAC
                  ************************************************************

HsCD00514241      AGCCTGCAGCACAAGGCCGCCAGCTCCATCTACATCAACTCACGCAGCGTCTTCCTGCGC
NM_004055.4       AGCCTGCAGCACAAGGCCGCCAGCTCCATCTACATCAACTCACGCAGCGTCTTCCTGCGC
                  ************************************************************

HsCD00514241      ACCGACCAGCCCGAGGGCCGCTATGTCATCATCCCCACAACCTTCGAGCCAGGCCACACT
NM_004055.4       ACCGACCAGCCCGAGGGCCGCTATGTCATCATCCCCACAACCTTCGAGCCAGGCCACACT
                  ************************************************************

HsCD00514241      GGCGAGTTCCTGCTCCGAGTCTTCACTGATGTGCCCTCCAACTGCCGGGAGCTGCGCCTG
NM_004055.4       GGCGAGTTCCTGCTCCGAGTCTTCACTGATGTGCCCTCCAACTGCCGGGAGCTGCGCCTG
                  ************************************************************

HsCD00514241      GATGAGCCCCCACACACCTGCTGGAGCTCCCTCTGTGGCTACCCCCAGCTGGTGACCCAG
NM_004055.4       GATGAGCCCCCACACACCTGCTGGAGCTCCCTCTGTGGCTACCCCCAGCTGGTGACCCAG
                  ************************************************************

HsCD00514241      GTACATGTCCTGGGAGCTGCTGGCCTCAAGGACTCCCCAACAGGGGCTAACTCTTATGTG
NM_004055.4       GTACATGTCCTGGGAGCTGCTGGCCTCAAGGACTCCCCAACAGGGGCTAACTCTTATGTG
                  ************************************************************

HsCD00514241      ATCATCAAGTGTGAGGGAGACAAAGTCCGCTCGGCTGTGCAGAAGGGCACCTCCACACCA
NM_004055.4       ATCATCAAGTGTGAGGGAGACAAAGTCCGCTCGGCTGTGCAGAAGGGCACCTCCACACCA
                  ************************************************************

HsCD00514241      GAGTACAATGTGAAAGGCATCTTCTACCGCAAGAAGCTGAGCCAGCCCATCACTGTACAG
NM_004055.4       GAGTACAATGTGAAAGGCATCTTCTACCGCAAGAAGCTGAGCCAGCCCATCACTGTACAG
                  ************************************************************

HsCD00514241      GTCTGGAACCACCGAGTGCTGAAGGATGAATTTCTGGGCCAGGTGCACCTAAAGGCTGAC
NM_004055.4       GTCTGGAACCACCGAGTGCTGAAGGATGAATTTCTGGGCCAGGTGCACCTAAAGGCTGAC
                  ************************************************************

HsCD00514241      CCGGACAACCTCCAGGCCCTGCATACCCTCCACCTCCGGGACCGAAATAGCCGGCAGCCC
NM_004055.4       CCGGACAACCTCCAGGCCCTGCATACCCTCCACCTCCGGGACCGAAATAGCCGGCAGCCC
                  ************************************************************

HsCD00514241      AGCAACCTGCCAGGCACTGTGGCCGTGCACATTCTCAGCAGCACCTCCCTCATGGCTGTC
NM_004055.4       AGCAACCTGCCAGGCACTGTGGCCGTGCACATTCTCAGCAGCACCTCCCTCATGGCTGTC
                  ************************************************************

HsCD00514241      ---
NM_004055.4       TGA
                     

Pathways

Panther : Huntington disease
WikiPathway : Integrin-mediated cell adhesion

Clone

Cloning Information : 606657
Original Clone ID : ccsbBroadEn_00187

Organism


TAX_ID : 9606
Species Specific ID: 726

Genome

Chromosome : 11
Map Location : 11q14
Ensembl : ENSG00000149260

Reagents

Labome : CAPN5-antibody