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CAPN6 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN6
Gene Name:  calpain 6
Sequence             
Original Clone ID: ccsbBroadEn_00217
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC000730.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00514282

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1924nts         Open reading frame : 1 to 1924
Reference Sequence Alignment

ATGGGTCCTCCTCTGAAGCTCTTCAAAAACCAGAAATACCAGGAACTGAAGCAGGAATGC
ATCAAAGACAGCAGACTTTTCTGTGATCCAACATTTCTGCCTGAGAATGATTCTCTTTTC
TACAACCGACTGCTTCCTGGAAAGGTGGTGTGGAAACGTCCCCAGGACATCTGTGATGAC
CCCCATCTGATTGTGGGCAACATTAGCAACCACCAGCTGACCCAAGGGAGACTGGGGCAC
AAGCCAATGGTTTCTGCATTTTCCTGTTTGGCTGTTCAGGAGTCTCATTGGACAAAGACA
ATTCCCAACCATAAGGAACAGGAATGGGACCCTCAAAAAACAGAAAAATACGCTGGGATA
TTTCACTTTCGTTTCTGGCATTTTGGAGAATGGACTGAAGTGGTGATTGATGACTTGTTG
CCCACCATTAACGGAGATCTGGTCTTCTCTTTCTCCACTTCCATGAATGAGTTTTGGAAT
GCTCTGCTGGAAAAAGCTTATGCAAAGCTGCTAGGCTGTTATGAGGCCCTGGATGGTTTG
ACCATCACTGATATTATTGTGGACTTCACGGGCACATTGGCTGAAACTGTTGACATGCAG
AAAGGAAGATACACTGAGCTTGTTGAGGAGAAGTACAAGCTATTCGGAGAACTGTACAAA
ACATTTACCAAAGGTGGTCTGATCTGCTGTTCCATTGAGTCTCCCAATCAGGAGGAGCAA
GAAGTTGAAACTGATTGGGGTCTGCTGAAGGGCCATACCTATACCATGACTGATATTCGC
AAAATTCGTCTTGGAGAGAGACTTGTGGAAGTCTTCAGTGCTGAGAAGGTGTATATGGTT
CGCCTGAGAAACCCCTTGGGAAGACAGGAATGGAGTGGCCCCTGGAGTGAAATTTCTGAA
GAGTGGCAGCAACTGACTGCATCAGATCGCAAGAACCTGGGGCTTGTTATGTCTGATGAT
GGAGAGTTTTGGATGAGCTTGGAGGACTTTTGCCGCAACTTTCACAAACTGAATGTCTGC
CGCAATGTGAACAACCCTATTTTTGGCCGAAAGGAGCTGGAATCGGTGTTGGGATGCTGG
ACTGTGGATGATGATCCCCTGATGAACCGCTCAGGAGGCTGCTATAACAACCGTGATACC
TTCCTGCAGAATCCCCAGTACATCTTCACTGTGCCTGAGGATGGGCACAAGGTCATTATG
TCACTGCAGCAGAAGGACCTGCGCACTTACCGCCGAATGGGAAGACCTGACAATTACATC
ATTGGCTTTGAGCTCTTCAAGGTGGAGATGAACCGCAAATTCCGCCTCCACCACCTCTAC
ATCCAGGAGCGTGCTGGGACTTCCACCTATATTGACACCCGCACAGTGTTTCTGAGCAAG
TACCTGAAGAAGGGCAACTATGTGCTTGTCCCAACCATGTTCCAGCATGGTCGCACCAGC
GAGTTTCTCCTGAGAATCTTCTCTGAAGTGCCTGTCCAGCTCAGGGAACTGACTCTGGAC
ATGCCCAAAATGTCCTGCTGGAACCTGGCTCGTGGCTACCCGAAAGTAGTTACTCAGATC
ACTGTTCACAGTGCTGAGGACCTGGAGAAGAAGTATGCCAATGAAACTGTAAACCCATAT
TTGGTCATCAAATGTGGAAAGGAGGAAGTCCGTTCTCCTGTCCAGAAGAATACAGTTCAT
GCCATTTTTGACACCCAGGCCATTTTCTACAGAAGGACCACTGACATTCCTATTATAGTA
CAGGTCTGGAACAGCCGAAAATTCTGTGATCAGTTCTTGGGGCAGGTTACTCTGGATGCT
GACCCCAGCGACTGCCGTGATCTGAAGTCTCTGTACCTGCGTAAGAAGGGTGGTCCAACT
GCCAAAGTCAAGCAAGGCCACATCAGCTTCAAGGTTATTTCCAGCGATGATCTCACTGAG
CTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN6
Symbol Nomenclature : CAPN6
SYNONYM : CANPX; CAPNX; CalpM; DJ914P14.1
Designation : calpain-6|calpain-like protease X-linked|calpamodulin
Full Nomenclature : calpain 6
GENEID : 827
GenBank Accession : DQ895041
HGNC : 1483
MIM : 300146
Vega : OTTHUMG00000022203
Target GenBank: DQ895041

 

Reference Sequence Alignment

  

HsCD00514282      ATGGGTCCTCCTCTGAAGCTCTTCAAAAACCAGAAATACCAGGAACTGAAGCAGGAATGC
NM_014289.3       ATGGGTCCTCCTCTGAAGCTCTTCAAAAACCAGAAATACCAGGAACTGAAGCAGGAATGC
                  ************************************************************

HsCD00514282      ATCAAAGACAGCAGACTTTTCTGTGATCCAACATTTCTGCCTGAGAATGATTCTCTTTTC
NM_014289.3       ATCAAAGACAGCAGACTTTTCTGTGATCCAACATTTCTGCCTGAGAATGATTCTCTTTTC
                  ************************************************************

HsCD00514282      TACAACCGACTGCTTCCTGGAAAGGTGGTGTGGAAACGTCCCCAGGACATCTGTGATGAC
NM_014289.3       TACAACCGACTGCTTCCTGGAAAGGTGGTGTGGAAACGTCCCCAGGACATCTGTGATGAC
                  ************************************************************

HsCD00514282      CCCCATCTGATTGTGGGCAACATTAGCAACCACCAGCTGACCCAAGGGAGACTGGGGCAC
NM_014289.3       CCCCATCTGATTGTGGGCAACATTAGCAACCACCAGCTGACCCAAGGGAGACTGGGGCAC
                  ************************************************************

HsCD00514282      AAGCCAATGGTTTCTGCATTTTCCTGTTTGGCTGTTCAGGAGTCTCATTGGACAAAGACA
NM_014289.3       AAGCCAATGGTTTCTGCATTTTCCTGTTTGGCTGTTCAGGAGTCTCATTGGACAAAGACA
                  ************************************************************

HsCD00514282      ATTCCCAACCATAAGGAACAGGAATGGGACCCTCAAAAAACAGAAAAATACGCTGGGATA
NM_014289.3       ATTCCCAACCATAAGGAACAGGAATGGGACCCTCAAAAAACAGAAAAATACGCTGGGATA
                  ************************************************************

HsCD00514282      TTTCACTTTCGTTTCTGGCATTTTGGAGAATGGACTGAAGTGGTGATTGATGACTTGTTG
NM_014289.3       TTTCACTTTCGTTTCTGGCATTTTGGAGAATGGACTGAAGTGGTGATTGATGACTTGTTG
                  ************************************************************

HsCD00514282      CCCACCATTAACGGAGATCTGGTCTTCTCTTTCTCCACTTCCATGAATGAGTTTTGGAAT
NM_014289.3       CCCACCATTAACGGAGATCTGGTCTTCTCTTTCTCCACTTCCATGAATGAGTTTTGGAAT
                  ************************************************************

HsCD00514282      GCTCTGCTGGAAAAAGCTTATGCAAAGCTGCTAGGCTGTTATGAGGCCCTGGATGGTTTG
NM_014289.3       GCTCTGCTGGAAAAAGCTTATGCAAAGCTGCTAGGCTGTTATGAGGCCCTGGATGGTTTG
                  ************************************************************

HsCD00514282      ACCATCACTGATATTATTGTGGACTTCACGGGCACATTGGCTGAAACTGTTGACATGCAG
NM_014289.3       ACCATCACTGATATTATTGTGGACTTCACGGGCACATTGGCTGAAACTGTTGACATGCAG
                  ************************************************************

HsCD00514282      AAAGGAAGATACACTGAGCTTGTTGAGGAGAAGTACAAGCTATTCGGAGAACTGTACAAA
NM_014289.3       AAAGGAAGATACACTGAGCTTGTTGAGGAGAAGTACAAGCTATTCGGAGAACTGTACAAA
                  ************************************************************

HsCD00514282      ACATTTACCAAAGGTGGTCTGATCTGCTGTTCCATTGAGTCTCCCAATCAGGAGGAGCAA
NM_014289.3       ACATTTACCAAAGGTGGTCTGATCTGCTGTTCCATTGAGTCTCCCAATCAGGAGGAGCAA
                  ************************************************************

HsCD00514282      GAAGTTGAAACTGATTGGGGTCTGCTGAAGGGCCATACCTATACCATGACTGATATTCGC
NM_014289.3       GAAGTTGAAACTGATTGGGGTCTGCTGAAGGGCCATACCTATACCATGACTGATATTCGC
                  ************************************************************

HsCD00514282      AAAATTCGTCTTGGAGAGAGACTTGTGGAAGTCTTCAGTGCTGAGAAGGTGTATATGGTT
NM_014289.3       AAAATTCGTCTTGGAGAGAGACTTGTGGAAGTCTTCAGTGCTGAGAAGGTGTATATGGTT
                  ************************************************************

HsCD00514282      CGCCTGAGAAACCCCTTGGGAAGACAGGAATGGAGTGGCCCCTGGAGTGAAATTTCTGAA
NM_014289.3       CGCCTGAGAAACCCCTTGGGAAGACAGGAATGGAGTGGCCCCTGGAGTGAAATTTCTGAA
                  ************************************************************

HsCD00514282      GAGTGGCAGCAACTGACTGCATCAGATCGCAAGAACCTGGGGCTTGTTATGTCTGATGAT
NM_014289.3       GAGTGGCAGCAACTGACTGCATCAGATCGCAAGAACCTGGGGCTTGTTATGTCTGATGAT
                  ************************************************************

HsCD00514282      GGAGAGTTTTGGATGAGCTTGGAGGACTTTTGCCGCAACTTTCACAAACTGAATGTCTGC
NM_014289.3       GGAGAGTTTTGGATGAGCTTGGAGGACTTTTGCCGCAACTTTCACAAACTGAATGTCTGC
                  ************************************************************

HsCD00514282      CGCAATGTGAACAACCCTATTTTTGGCCGAAAGGAGCTGGAATCGGTGTTGGGATGCTGG
NM_014289.3       CGCAATGTGAACAACCCTATTTTTGGCCGAAAGGAGCTGGAATCGGTGTTGGGATGCTGG
                  ************************************************************

HsCD00514282      ACTGTGGATGATGATCCCCTGATGAACCGCTCAGGAGGCTGCTATAACAACCGTGATACC
NM_014289.3       ACTGTGGATGATGATCCCCTGATGAACCGCTCAGGAGGCTGCTATAACAACCGTGATACC
                  ************************************************************

HsCD00514282      TTCCTGCAGAATCCCCAGTACATCTTCACTGTGCCTGAGGATGGGCACAAGGTCATTATG
NM_014289.3       TTCCTGCAGAATCCCCAGTACATCTTCACTGTGCCTGAGGATGGGCACAAGGTCATTATG
                  ************************************************************

HsCD00514282      TCACTGCAGCAGAAGGACCTGCGCACTTACCGCCGAATGGGAAGACCTGACAATTACATC
NM_014289.3       TCACTGCAGCAGAAGGACCTGCGCACTTACCGCCGAATGGGAAGACCTGACAATTACATC
                  ************************************************************

HsCD00514282      ATTGGCTTTGAGCTCTTCAAGGTGGAGATGAACCGCAAATTCCGCCTCCACCACCTCTAC
NM_014289.3       ATTGGCTTTGAGCTCTTCAAGGTGGAGATGAACCGCAAATTCCGCCTCCACCACCTCTAC
                  ************************************************************

HsCD00514282      ATCCAGGAGCGTGCTGGGACTTCCACCTATATTGACACCCGCACAGTGTTTCTGAGCAAG
NM_014289.3       ATCCAGGAGCGTGCTGGGACTTCCACCTATATTGACACCCGCACAGTGTTTCTGAGCAAG
                  ************************************************************

HsCD00514282      TACCTGAAGAAGGGCAACTATGTGCTTGTCCCAACCATGTTCCAGCATGGTCGCACCAGC
NM_014289.3       TACCTGAAGAAGGGCAACTATGTGCTTGTCCCAACCATGTTCCAGCATGGTCGCACCAGC
                  ************************************************************

HsCD00514282      GAGTTTCTCCTGAGAATCTTCTCTGAAGTGCCTGTCCAGCTCAGGGAACTGACTCTGGAC
NM_014289.3       GAGTTTCTCCTGAGAATCTTCTCTGAAGTGCCTGTCCAGCTCAGGGAACTGACTCTGGAC
                  ************************************************************

HsCD00514282      ATGCCCAAAATGTCCTGCTGGAACCTGGCTCGTGGCTACCCGAAAGTAGTTACTCAGATC
NM_014289.3       ATGCCCAAAATGTCCTGCTGGAACCTGGCTCGTGGCTACCCGAAAGTAGTTACTCAGATC
                  ************************************************************

HsCD00514282      ACTGTTCACAGTGCTGAGGACCTGGAGAAGAAGTATGCCAATGAAACTGTAAACCCATAT
NM_014289.3       ACTGTTCACAGTGCTGAGGACCTGGAGAAGAAGTATGCCAATGAAACTGTAAACCCATAT
                  ************************************************************

HsCD00514282      TTGGTCATCAAATGTGGAAAGGAGGAAGTCCGTTCTCCTGTCCAGAAGAATACAGTTCAT
NM_014289.3       TTGGTCATCAAATGTGGAAAGGAGGAAGTCCGTTCTCCTGTCCAGAAGAATACAGTTCAT
                  ************************************************************

HsCD00514282      GCCATTTTTGACACCCAGGCCATTTTCTACAGAAGGACCACTGACATTCCTATTATAGTA
NM_014289.3       GCCATTTTTGACACCCAGGCCATTTTCTACAGAAGGACCACTGACATTCCTATTATAGTA
                  ************************************************************

HsCD00514282      CAGGTCTGGAACAGCCGAAAATTCTGTGATCAGTTCTTGGGGCAGGTTACTCTGGATGCT
NM_014289.3       CAGGTCTGGAACAGCCGAAAATTCTGTGATCAGTTCTTGGGGCAGGTTACTCTGGATGCT
                  ************************************************************

HsCD00514282      GACCCCAGCGACTGCCGTGATCTGAAGTCTCTGTACCTGCGTAAGAAGGGTGGTCCAACT
NM_014289.3       GACCCCAGCGACTGCCGTGATCTGAAGTCTCTGTACCTGCGTAAGAAGGGTGGTCCAACT
                  ************************************************************

HsCD00514282      GCCAAAGTCAAGCAAGGCCACATCAGCTTCAAGGTTATTTCCAGCGATGATCTCACTGAG
NM_014289.3       GCCAAAGTCAAGCAAGGCCACATCAGCTTCAAGGTTATTTCCAGCGATGATCTCACTGAG
                  ************************************************************

HsCD00514282      CTC---
NM_014289.3       CTCTAA
                  ***   

Pathways

Panther : Huntington disease
WikiPathway : Integrin-mediated cell adhesion

Clone

Cloning Information : 606698
Original Clone ID : ccsbBroadEn_00217

Organism


TAX_ID : 9606
Species Specific ID: 827

Genome

Chromosome : X
Map Location : Xq23
Ensembl : ENSG00000077274

Reagents

Labome : CAPN6-antibody