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LRRC32 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LRRC32
Gene Name:  leucine rich repeat containing 32
Sequence             
Original Clone ID: ccsbBroadEn_06255
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC070079.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00514381

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1987nts         Open reading frame : 1 to 1987
Reference Sequence Alignment

ATGAGACCCCAGATCCTGCTGCTCCTGGCCCTGCTGACCCTAGGCCTGGCTGCACAACAC
CAAGACAAAGTGCCCTGTAAGATGGTGGACAAGAAGGTCTCGTGCCAGGTTCTGGGCCTG
CTCCAGGTCCCCTCGGTGCTCCCGCCAGACACTGAGACCCTTGATCTATCTGGGAACCAG
CTGCGGAGTATCCTGGCCTCACCCCTGGGCTTCTACACGGCACTTCGTCACCTGGACCTG
AGCACCAATGAGATCAGCTTCCTCCAGCCAGGAGCCTTCCAGGCCCTGACCCACCTGGAG
CACCTCAGCCTGGCTCACAACCGGCTGGCGATGGCCACTGCGCTGAGTGCTGGTGGCCTG
GGCCCCCTGCCACGCGTGACCTCCCTGGACCTGTCTGGGAACAGCCTGTACAGCGGCCTG
CTGGAGCGGCTGCTGGGGGAGGCACCCAGCCTGCATACCCTCTCACTGGCGGAGAACAGT
CTGACTCGCCTCACCCGCCACACCTTCCGGGACATGCCTGCGCTGGAGCAGCTTGACCTG
CATAGCAACGTGCTGATGGACATCGAGGATGGCGCCTTCGAGGGTCTGCCCCGCCTGACC
CATCTCAACCTCTCCAGGAATTCCCTCACCTGCATCTCCGACTTCAGCCTCCAGCAGCTG
CGGGTGCTAGACCTGAGCTGCAACAGCATCGAGGCCTTTCAGACGGCCTCCCAGCCCCAG
GCTGAGTTCCAGCTCACCTGGCTTGACCTGCGGGAGAACAAACTGCTCCATTTCCCCGAC
CTGGCCGCGCTCCCGAGACTCATCTACCTGAACTTGTCCAACAACCTCATCCGGCTCCCC
ACAGGGCCACCCCAGGACAGCAAGGGCATCCACGCACCTTCCGAGGGCTGGTCAGCCCTG
CCCCTCTCAGCCCCCAGCGGGAATGCCAGCGGCCGCCCCCTTTCCCAGCTCTTGAATCTG
GATTTGAGCTACAATGAGATTGAGCTCATCCCCGACAGCTTTCTTGAGCACCTGACCTCC
CTGTGCTTCCTGAACCTCAGCAGAAACTGCTTGCGGACCTTTGAGGCCCGGCGCTTAGGC
TCCCTGCCCTGCCTGATGCTCCTTGACTTAAGCCACAATGCCCTGGAGACACTGGAACTG
GGCGCCAGAGCCCTGGGGTCTCTGCGGACGCTGCTCCTACAGGGCAATGCCCTGCGGGAC
CTGCCCCCATACACCTTTGCCAATCTGGCCAGCCTGCAGCGGCTCAACCTGCAGGGGAAC
CGAGTCAGCCCCTGTGGGGGGCCAGATGAGCCTGGCCCCTCCGGCTGTGTGGCCTTCTCC
GGCATCACCTCCCTCCGCAGCCTGAGCCTGGTGGATAATGAGATAGAGCTGCTCAGGGCA
GGGGCCTTCCTCCACACCCCACTGACTGAGCTGGACCTTTCTTCCAATCCTGGGCTGGAG
GTGGCCACGGGGGCCTTGGGAGGCCTGGAGGCCTCCTTGGAGGTCCTGGCACTGCAGGGC
AACGGGCTGATGGTCCTGCAGGTGGACCTGCCCTGCTTCATCTGCCTCAAGCGGCTCAAT
CTTGCCGAGAACCGCCTGAGCCACCTTCCCGCCTGGACACAGGCTGTGTCACTGGAGGTG
CTGGACCTGCGAAACAACAGCTTCAGCCTCCTGCCAGGCAGTGCCATGGGTGGCCTGGAG
ACCAGCCTCCGGCGCCTCTACCTGCAGGGGAATCCACTCAGCTGCTGCGGCAATGGCTGG
CTGGCAGCCCAGCTGCACCAGGGCCGTGTGGACGTGGACGCCACCCAGGACCTGATCTGC
CGCTTCAGCTCCCAGGAGGAGGTGTCCCTGAGCCACGTGCGTCCCGAAGACTGTGAGAAG
GGGGGACTGAAGAACATCAACCTCATCATCATCCTCACCTTCATACTGGTCTCTGCCATC
CTCCTCACCACGCTGGCCGCCTGCTGCTGCGTCCGCCGGCAGAAGTTTAACCAACAGTAT
AAAGCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LRRC32
Symbol Nomenclature : LRRC32
SYNONYM : D11S833E; GARP
Designation : garpin|glycoprotein A repetitions predominant|leucine-rich repeat-containing protein 32
Full Nomenclature : leucine rich repeat containing 32
GENEID : 2615
HGNC : 4161
MIM : 137207
Vega : OTTHUMG00000133687
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00514381        ATGAGACCCCAGATCCTGCTGCTCCTGGCCCTGCTGACCCTAGGCCTGGCTGCACAACAC
NM_005512.2         ATGAGACCCCAGATCCTGCTGCTCCTGGCCCTGCTGACCCTAGGCCTGGCTGCACAACAC
NM_001128922.1      ATGAGACCCCAGATCCTGCTGCTCCTGGCCCTGCTGACCCTAGGCCTGGCTGCACAACAC
                    ************************************************************

HsCD00514381        CAAGACAAAGTGCCCTGTAAGATGGTGGACAAGAAGGTCTCGTGCCAGGTTCTGGGCCTG
NM_005512.2         CAAGACAAAGTGCCCTGTAAGATGGTGGACAAGAAGGTCTCGTGCCAGGTTCTGGGCCTG
NM_001128922.1      CAAGACAAAGTGCCCTGTAAGATGGTGGACAAGAAGGTCTCGTGCCAGGTTCTGGGCCTG
                    ************************************************************

HsCD00514381        CTCCAGGTCCCCTCGGTGCTCCCGCCAGACACTGAGACCCTTGATCTATCTGGGAACCAG
NM_005512.2         CTCCAGGTCCCCTCGGTGCTCCCGCCAGACACTGAGACCCTTGATCTATCTGGGAACCAG
NM_001128922.1      CTCCAGGTCCCCTCGGTGCTCCCGCCAGACACTGAGACCCTTGATCTATCTGGGAACCAG
                    ************************************************************

HsCD00514381        CTGCGGAGTATCCTGGCCTCACCCCTGGGCTTCTACACGGCACTTCGTCACCTGGACCTG
NM_005512.2         CTGCGGAGTATCCTGGCCTCACCCCTGGGCTTCTACACAGCACTTCGTCACCTGGACCTG
NM_001128922.1      CTGCGGAGTATCCTGGCCTCACCCCTGGGCTTCTACACAGCACTTCGTCACCTGGACCTG
                    **************************************.*********************

HsCD00514381        AGCACCAATGAGATCAGCTTCCTCCAGCCAGGAGCCTTCCAGGCCCTGACCCACCTGGAG
NM_005512.2         AGCACCAATGAGATCAGCTTCCTCCAGCCAGGAGCCTTCCAGGCCCTGACCCACCTGGAG
NM_001128922.1      AGCACCAATGAGATCAGCTTCCTCCAGCCAGGAGCCTTCCAGGCCCTGACCCACCTGGAG
                    ************************************************************

HsCD00514381        CACCTCAGCCTGGCTCACAACCGGCTGGCGATGGCCACTGCGCTGAGTGCTGGTGGCCTG
NM_005512.2         CACCTCAGCCTGGCTCACAACCGGCTGGCGATGGCCACTGCGCTGAGTGCTGGTGGCCTG
NM_001128922.1      CACCTCAGCCTGGCTCACAACCGGCTGGCGATGGCCACTGCGCTGAGTGCTGGTGGCCTG
                    ************************************************************

HsCD00514381        GGCCCCCTGCCACGCGTGACCTCCCTGGACCTGTCTGGGAACAGCCTGTACAGCGGCCTG
NM_005512.2         GGCCCCCTGCCACGCGTGACCTCCCTGGACCTGTCTGGGAACAGCCTGTACAGCGGCCTG
NM_001128922.1      GGCCCCCTGCCACGCGTGACCTCCCTGGACCTGTCTGGGAACAGCCTGTACAGCGGCCTG
                    ************************************************************

HsCD00514381        CTGGAGCGGCTGCTGGGGGAGGCACCCAGCCTGCATACCCTCTCACTGGCGGAGAACAGT
NM_005512.2         CTGGAGCGGCTGCTGGGGGAGGCACCCAGCCTGCATACCCTCTCACTGGCGGAGAACAGT
NM_001128922.1      CTGGAGCGGCTGCTGGGGGAGGCACCCAGCCTGCATACCCTCTCACTGGCGGAGAACAGT
                    ************************************************************

HsCD00514381        CTGACTCGCCTCACCCGCCACACCTTCCGGGACATGCCTGCGCTGGAGCAGCTTGACCTG
NM_005512.2         CTGACTCGCCTCACCCGCCACACCTTCCGGGACATGCCTGCGCTGGAGCAGCTTGACCTG
NM_001128922.1      CTGACTCGCCTCACCCGCCACACCTTCCGGGACATGCCTGCGCTGGAGCAGCTTGACCTG
                    ************************************************************

HsCD00514381        CATAGCAACGTGCTGATGGACATCGAGGATGGCGCCTTCGAGGGTCTGCCCCGCCTGACC
NM_005512.2         CATAGCAACGTGCTGATGGACATCGAGGATGGCGCCTTCGAGGGTCTGCCCCGCCTGACC
NM_001128922.1      CATAGCAACGTGCTGATGGACATCGAGGATGGCGCCTTCGAGGGTCTGCCCCGCCTGACC
                    ************************************************************

HsCD00514381        CATCTCAACCTCTCCAGGAATTCCCTCACCTGCATCTCCGACTTCAGCCTCCAGCAGCTG
NM_005512.2         CATCTCAACCTCTCCAGGAATTCCCTCACCTGCATCTCCGACTTCAGCCTCCAGCAGCTG
NM_001128922.1      CATCTCAACCTCTCCAGGAATTCCCTCACCTGCATCTCCGACTTCAGCCTCCAGCAGCTG
                    ************************************************************

HsCD00514381        CGGGTGCTAGACCTGAGCTGCAACAGCATCGAGGCCTTTCAGACGGCCTCCCAGCCCCAG
NM_005512.2         CGGGTGCTAGACCTGAGCTGCAACAGCATCGAGGCCTTTCAGACGGCCTCCCAGCCCCAG
NM_001128922.1      CGGGTGCTAGACCTGAGCTGCAACAGCATCGAGGCCTTTCAGACGGCCTCCCAGCCCCAG
                    ************************************************************

HsCD00514381        GCTGAGTTCCAGCTCACCTGGCTTGACCTGCGGGAGAACAAACTGCTCCATTTCCCCGAC
NM_005512.2         GCTGAGTTCCAGCTCACCTGGCTTGACCTGCGGGAGAACAAACTGCTCCATTTCCCCGAC
NM_001128922.1      GCTGAGTTCCAGCTCACCTGGCTTGACCTGCGGGAGAACAAACTGCTCCATTTCCCCGAC
                    ************************************************************

HsCD00514381        CTGGCCGCGCTCCCGAGACTCATCTACCTGAACTTGTCCAACAACCTCATCCGGCTCCCC
NM_005512.2         CTGGCCGCGCTCCCGAGACTCATCTACCTGAACTTGTCCAACAACCTCATCCGGCTCCCC
NM_001128922.1      CTGGCCGCGCTCCCGAGACTCATCTACCTGAACTTGTCCAACAACCTCATCCGGCTCCCC
                    ************************************************************

HsCD00514381        ACAGGGCCACCCCAGGACAGCAAGGGCATCCACGCACCTTCCGAGGGCTGGTCAGCCCTG
NM_005512.2         ACAGGGCCACCCCAGGACAGCAAGGGCATCCACGCACCTTCCGAGGGCTGGTCAGCCCTG
NM_001128922.1      ACAGGGCCACCCCAGGACAGCAAGGGCATCCACGCACCTTCCGAGGGCTGGTCAGCCCTG
                    ************************************************************

HsCD00514381        CCCCTCTCAGCCCCCAGCGGGAATGCCAGCGGCCGCCCCCTTTCCCAGCTCTTGAATCTG
NM_005512.2         CCCCTCTCAGCCCCCAGCGGGAATGCCAGCGGCCGCCCCCTTTCCCAGCTCTTGAATCTG
NM_001128922.1      CCCCTCTCAGCCCCCAGCGGGAATGCCAGCGGCCGCCCCCTTTCCCAGCTCTTGAATCTG
                    ************************************************************

HsCD00514381        GATTTGAGCTACAATGAGATTGAGCTCATCCCCGACAGCTTTCTTGAGCACCTGACCTCC
NM_005512.2         GATTTGAGCTACAATGAGATTGAGCTCATCCCCGACAGCTTTCTTGAGCACCTGACCTCC
NM_001128922.1      GATTTGAGCTACAATGAGATTGAGCTCATCCCCGACAGCTTTCTTGAGCACCTGACCTCC
                    ************************************************************

HsCD00514381        CTGTGCTTCCTGAACCTCAGCAGAAACTGCTTGCGGACCTTTGAGGCCCGGCGCTTAGGC
NM_005512.2         CTGTGCTTCCTGAACCTCAGCAGAAACTGCTTGCGGACCTTTGAGGCCCGGCGCTTAGGC
NM_001128922.1      CTGTGCTTCCTGAACCTCAGCAGAAACTGCTTGCGGACCTTTGAGGCCCGGCGCTTAGGC
                    ************************************************************

HsCD00514381        TCCCTGCCCTGCCTGATGCTCCTTGACTTAAGCCACAATGCCCTGGAGACACTGGAACTG
NM_005512.2         TCCCTGCCCTGCCTGATGCTCCTTGACTTAAGCCACAATGCCCTGGAGACACTGGAACTG
NM_001128922.1      TCCCTGCCCTGCCTGATGCTCCTTGACTTAAGCCACAATGCCCTGGAGACACTGGAACTG
                    ************************************************************

HsCD00514381        GGCGCCAGAGCCCTGGGGTCTCTGCGGACGCTGCTCCTACAGGGCAATGCCCTGCGGGAC
NM_005512.2         GGCGCCAGAGCCCTGGGGTCTCTGCGGACGCTGCTCCTACAGGGCAATGCCCTGCGGGAC
NM_001128922.1      GGCGCCAGAGCCCTGGGGTCTCTGCGGACGCTGCTCCTACAGGGCAATGCCCTGCGGGAC
                    ************************************************************

HsCD00514381        CTGCCCCCATACACCTTTGCCAATCTGGCCAGCCTGCAGCGGCTCAACCTGCAGGGGAAC
NM_005512.2         CTGCCCCCATACACCTTTGCCAATCTGGCCAGCCTGCAGCGGCTCAACCTGCAGGGGAAC
NM_001128922.1      CTGCCCCCATACACCTTTGCCAATCTGGCCAGCCTGCAGCGGCTCAACCTGCAGGGGAAC
                    ************************************************************

HsCD00514381        CGAGTCAGCCCCTGTGGGGGGCCAGATGAGCCTGGCCCCTCCGGCTGTGTGGCCTTCTCC
NM_005512.2         CGAGTCAGCCCCTGTGGGGGGCCAGATGAGCCTGGCCCCTCCGGCTGTGTGGCCTTCTCC
NM_001128922.1      CGAGTCAGCCCCTGTGGGGGGCCAGATGAGCCTGGCCCCTCCGGCTGTGTGGCCTTCTCC
                    ************************************************************

HsCD00514381        GGCATCACCTCCCTCCGCAGCCTGAGCCTGGTGGATAATGAGATAGAGCTGCTCAGGGCA
NM_005512.2         GGCATCACCTCCCTCCGCAGCCTGAGCCTGGTGGATAATGAGATAGAGCTGCTCAGGGCA
NM_001128922.1      GGCATCACCTCCCTCCGCAGCCTGAGCCTGGTGGATAATGAGATAGAGCTGCTCAGGGCA
                    ************************************************************

HsCD00514381        GGGGCCTTCCTCCACACCCCACTGACTGAGCTGGACCTTTCTTCCAATCCTGGGCTGGAG
NM_005512.2         GGGGCCTTCCTCCACACCCCACTGACTGAGCTGGACCTTTCTTCCAATCCTGGGCTGGAG
NM_001128922.1      GGGGCCTTCCTCCACACCCCACTGACTGAGCTGGACCTTTCTTCCAATCCTGGGCTGGAG
                    ************************************************************

HsCD00514381        GTGGCCACGGGGGCCTTGGGAGGCCTGGAGGCCTCCTTGGAGGTCCTGGCACTGCAGGGC
NM_005512.2         GTGGCCACGGGGGCCTTGGGAGGCCTGGAGGCCTCCTTGGAGGTCCTGGCACTGCAGGGC
NM_001128922.1      GTGGCCACGGGGGCCTTGGGAGGCCTGGAGGCCTCCTTGGAGGTCCTGGCACTGCAGGGC
                    ************************************************************

HsCD00514381        AACGGGCTGATGGTCCTGCAGGTGGACCTGCCCTGCTTCATCTGCCTCAAGCGGCTCAAT
NM_005512.2         AACGGGCTGATGGTCCTGCAGGTGGACCTGCCCTGCTTCATCTGCCTCAAGCGGCTCAAT
NM_001128922.1      AACGGGCTGATGGTCCTGCAGGTGGACCTGCCCTGCTTCATCTGCCTCAAGCGGCTCAAT
                    ************************************************************

HsCD00514381        CTTGCCGAGAACCGCCTGAGCCACCTTCCCGCCTGGACACAGGCTGTGTCACTGGAGGTG
NM_005512.2         CTTGCCGAGAACCGCCTGAGCCACCTTCCCGCCTGGACACAGGCTGTGTCACTGGAGGTG
NM_001128922.1      CTTGCCGAGAACCGCCTGAGCCACCTTCCCGCCTGGACACAGGCTGTGTCACTGGAGGTG
                    ************************************************************

HsCD00514381        CTGGACCTGCGAAACAACAGCTTCAGCCTCCTGCCAGGCAGTGCCATGGGTGGCCTGGAG
NM_005512.2         CTGGACCTGCGAAACAACAGCTTCAGCCTCCTGCCAGGCAGTGCCATGGGTGGCCTGGAG
NM_001128922.1      CTGGACCTGCGAAACAACAGCTTCAGCCTCCTGCCAGGCAGTGCCATGGGTGGCCTGGAG
                    ************************************************************

HsCD00514381        ACCAGCCTCCGGCGCCTCTACCTGCAGGGGAATCCACTCAGCTGCTGCGGCAATGGCTGG
NM_005512.2         ACCAGCCTCCGGCGCCTCTACCTGCAGGGGAATCCACTCAGCTGCTGCGGCAATGGCTGG
NM_001128922.1      ACCAGCCTCCGGCGCCTCTACCTGCAGGGGAATCCACTCAGCTGCTGCGGCAATGGCTGG
                    ************************************************************

HsCD00514381        CTGGCAGCCCAGCTGCACCAGGGCCGTGTGGACGTGGACGCCACCCAGGACCTGATCTGC
NM_005512.2         CTGGCAGCCCAGCTGCACCAGGGCCGTGTGGACGTGGACGCCACCCAGGACCTGATCTGC
NM_001128922.1      CTGGCAGCCCAGCTGCACCAGGGCCGTGTGGACGTGGACGCCACCCAGGACCTGATCTGC
                    ************************************************************

HsCD00514381        CGCTTCAGCTCCCAGGAGGAGGTGTCCCTGAGCCACGTGCGTCCCGAAGACTGTGAGAAG
NM_005512.2         CGCTTCAGCTCCCAGGAGGAGGTGTCCCTGAGCCACGTGCGTCCCGAGGACTGTGAGAAG
NM_001128922.1      CGCTTCAGCTCCCAGGAGGAGGTGTCCCTGAGCCACGTGCGTCCCGAGGACTGTGAGAAG
                    ***********************************************.************

HsCD00514381        GGGGGACTGAAGAACATCAACCTCATCATCATCCTCACCTTCATACTGGTCTCTGCCATC
NM_005512.2         GGGGGACTGAAGAACATCAACCTCATCATCATCCTCACCTTCATACTGGTCTCTGCCATC
NM_001128922.1      GGGGGACTGAAGAACATCAACCTCATCATCATCCTCACCTTCATACTGGTCTCTGCCATC
                    ************************************************************

HsCD00514381        CTCCTCACCACGCTGGCCGCCTGCTGCTGCGTCCGCCGGCAGAAGTTTAACCAACAGTAT
NM_005512.2         CTCCTCACCACGCTGGCCGCCTGCTGCTGCGTCCGCCGGCAGAAGTTTAACCAACAGTAT
NM_001128922.1      CTCCTCACCACGCTGGCCGCCTGCTGCTGCGTCCGCCGGCAGAAGTTTAACCAACAGTAT
                    ************************************************************

HsCD00514381        AAAGCC---
NM_005512.2         AAAGCCTAA
NM_001128922.1      AAAGCCTAA
                    ******   

Clone

Cloning Information : 665946
Original Clone ID : ccsbBroadEn_06255

Organism


TAX_ID : 9606
Species Specific ID: 2615

Genome

Chromosome : 11
Map Location : 11q13.5-q14
Ensembl : ENSG00000137507

Reagents

Labome : GARP-antibody