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CAPN13 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN13
Gene Name:  calpain 13
Sequence             
Original Clone ID: ccsbBroadEn_09334
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC117345.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00514516

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2008nts         Open reading frame : 1 to 2008
Reference Sequence Alignment

ATGGCGTATTACCAGGAGCCTTCAGTGGAGACCTCCATCATCAAGTTCAAAGACCAGGAC
TTTACCACCTTGCGGGATCACTGCCTGAGCATGGGCCGGACGTTTAAGGATGAGACATTC
CCTGCAGCAGATTCTTCCATAGGCCAGAAGCTGCTCCAGGAAAAACGCCTCTCCAATGTG
ATATGGAAGCGGCCACAGGATCTACCAGGGGGTCCTCCTCACTTCATCCTGGATGATATA
AGCAGATTTGACATCCAACAAGGAGGCGCAGCTGACTGCTGGTTCCTGGCAGCACTGGGA
TCCTTGACTCAGAACCCACAGTACAGGCAGAAGATCCTGATGGTCCAAAGCTTTTCACAC
CAGTATGCTGGCATTTTCCGTTTCCGGTTCTGGCAATGTGGCCAGTGGGTGGAAGTGGTG
ATTGATGACCGCCTACCTGTCCAGGGAGATAAATGCCTCTTTGTGCGTCCTCGCCACCAA
AACCAAGAGTTCTGGCCCTGCCTGCTGGAGAAGGCCTATGCCAAGCTGCTCGGATCCTAT
TCCGATCTGCACTATGGCTTCCTCGAGGATGCCCTGGTGGACCTCACAGGAGGCGTGATC
ACCAACATCCATCTGCACTCTTCCCCTGTGGACCTGGTGAAGGCAGTGAAGACAGCGACC
AAGGCAGGCTCCCTGATAACCTGTGCCACTCCAAGTGGGCCAACAGATACAGCACAGGCG
ATGGAGAATGGGCTGGTGAGTCTCCATGCCTACACTGTGACTGGGGCTGAGCAGATTCAA
TACCGAAGGGGCTGGGAAGAAATTATCTCCCTGTGGAACCCCTGGGGCTGGGGCGAGACC
GAATGGAGAGGGCGCTGGAGTGATGGGTCTCAGGAGTGGGAGGAAACCTGTGATCCGCGG
AAAAGCCAGCTACATAAGAAACGGGAAGATGGCGAGTTTTGGATGTCATGTCAAGATTTC
CAACAGAAATTCATCGCCATGTTTATATGTAGCGAAATTCCAATTACCCTGGACCATGGA
AACACACTCCACGAAGGATGGTCCCAAATAATGTTTAGGAAGCAAGTGATTCTAGGAAAC
ACTGCAGGAGGACCTCGGAATGATGCTCAATTCAACTTCTCTGTGCAAGAGCCAATGGAA
GGCACCAATGTTGTCGTGTGCGTCACAGTTGCTGTCACACCATCAAATTTGAAAGCAGAA
GATGCAAAATTTCCACTCGATTTCCAAGTGATTCTGGCTGGCTCACAGCGGTTCCGGGAG
AAATTTCCACCCGTGTTTTTTTCCTCGTTCAGAAACACTGTCCAAAGCTCAAATAATAAA
TTCCGCCGCAACTTCACCATGACTTACCATCTGAGCCCTGGGAACTATGTTGTGGTTGCA
CAGACACGGAGAAAATCAGCGGAGTTCTTGCTCCGAATCTTCCTGAAAATGCCAGACAGT
GACAGGCACCTGAGCAGCCATTTCAACCTCAGAATGAAGGGAAGCCCTTCAGAACATGGC
TCCCAACAAAGCATTTTCAACAGATATGCTCAGCAGAGGCTGGACATTGATGCCACCCAG
CTTCAGGGCCTTCTCAACCAGGAGCTTCTAACAGGACCTCCAGGGGACATGTTCTCCTTA
GATGAGTGCCGCAGCTTGGTGGCTCTGATGGAACTGAAAGTGAATGGGCGGCTAGACCAA
GAGGAGTTTGCGCGACTGTGGAAGCGCCTTGTTCACTACCAGCATGTTTTCCAGAAGGTT
CAGACAAGCCCTGGAGTCCTCCTGAGCTCGGACTTGTGGAAGGCCATAGAGAATACAGAC
TTCCTCAGAGGGATCTTCATCAGCCGTGAGCTGCTGCATCTGGTGACCCTCAGGTACAGC
GACAGCGTCGGCAGGGTCAGCTTCCCCAGCCTGGTCTGCTTCCTGATGCGGCTTGAAGCC
ATGGCAAAGACCTTCCGCAACCTCTCTAAGGATGGAAAAGGACTCTACCTGACAGAAATG
GAGTGGATGAGCCTGGTCATGTACAAC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN13
Symbol Nomenclature : CAPN13
Designation : CANP 13|calcium-activated neutral proteinase 13|calpain-13
Full Nomenclature : calpain 13
GENEID : 92291
GenBank Accession : HQ258338
HGNC : 16663
MIM : 610228
Vega : OTTHUMG00000152053
Target GenBank: HQ258338

 

Reference Sequence Alignment

  

HsCD00514516      ATGGCGTATTACCAGGAGCCTTCAGTGGAGACCTCCATCATCAAGTTCAAAGACCAGGAC
NM_144575.2       ATGGCGTATTACCAGGAGCCTTCAGTGGAGACCTCCATCATCAAGTTCAAAGACCAGGAC
                  ************************************************************

HsCD00514516      TTTACCACCTTGCGGGATCACTGCCTGAGCATGGGCCGGACGTTTAAGGATGAGACATTC
NM_144575.2       TTTACCACCTTGCGGGATCACTGCCTGAGCATGGGCCGGACGTTTAAGGATGAGACATTC
                  ************************************************************

HsCD00514516      CCTGCAGCAGATTCTTCCATAGGCCAGAAGCTGCTCCAGGAAAAACGCCTCTCCAATGTG
NM_144575.2       CCTGCAGCAGATTCTTCCATAGGCCAGAAGCTGCTCCAGGAAAAACGCCTCTCCAATGTG
                  ************************************************************

HsCD00514516      ATATGGAAGCGGCCACAGGATCTACCAGGGGGTCCTCCTCACTTCATCCTGGATGATATA
NM_144575.2       ATATGGAAGCGGCCACAGGATCTACCAGGGGGTCCTCCTCACTTCATCCTGGATGATATA
                  ************************************************************

HsCD00514516      AGCAGATTTGACATCCAACAAGGAGGCGCAGCTGACTGCTGGTTCCTGGCAGCACTGGGA
NM_144575.2       AGCAGATTTGACATCCAACAAGGAGGCGCAGCTGACTGCTGGTTCCTGGCAGCACTGGGA
                  ************************************************************

HsCD00514516      TCCTTGACTCAGAACCCACAGTACAGGCAGAAGATCCTGATGGTCCAAAGCTTTTCACAC
NM_144575.2       TCCTTGACTCAGAACCCACAGTACAGGCAGAAGATCCTGATGGTCCAAAGCTTTTCACAC
                  ************************************************************

HsCD00514516      CAGTATGCTGGCATTTTCCGTTTCCGGTTCTGGCAATGTGGCCAGTGGGTGGAAGTGGTG
NM_144575.2       CAGTATGCTGGCATTTTCCGTTTCCGGTTCTGGCAATGTGGCCAGTGGGTGGAAGTGGTG
                  ************************************************************

HsCD00514516      ATTGATGACCGCCTACCTGTCCAGGGAGATAAATGCCTCTTTGTGCGTCCTCGCCACCAA
NM_144575.2       ATTGATGACCGCCTACCTGTCCAGGGAGATAAATGCCTCTTTGTGCGTCCTCGCCACCAA
                  ************************************************************

HsCD00514516      AACCAAGAGTTCTGGCCCTGCCTGCTGGAGAAGGCCTATGCCAAGCTGCTCGGATCCTAT
NM_144575.2       AACCAAGAGTTCTGGCCCTGCCTGCTGGAGAAGGCCTATGCCAAGCTGCTCGGATCCTAT
                  ************************************************************

HsCD00514516      TCCGATCTGCACTATGGCTTCCTCGAGGATGCCCTGGTGGACCTCACAGGAGGCGTGATC
NM_144575.2       TCCGATCTGCACTATGGCTTCCTCGAGGATGCCCTGGTGGACCTCACAGGAGGCGTGATC
                  ************************************************************

HsCD00514516      ACCAACATCCATCTGCACTCTTCCCCTGTGGACCTGGTGAAGGCAGTGAAGACAGCGACC
NM_144575.2       ACCAACATCCATCTGCACTCTTCCCCTGTGGACCTGGTGAAGGCAGTGAAGACAGCGACC
                  ************************************************************

HsCD00514516      AAGGCAGGCTCCCTGATAACCTGTGCCACTCCAAGTGGGCCAACAGATACAGCACAGGCG
NM_144575.2       AAGGCAGGCTCCCTGATAACCTGTGCCACTCCAAGTGGGCCAACAGATACAGCACAGGCG
                  ************************************************************

HsCD00514516      ATGGAGAATGGGCTGGTGAGTCTCCATGCCTACACTGTGACTGGGGCTGAGCAGATTCAA
NM_144575.2       ATGGAGAATGGGCTGGTGAGTCTCCATGCCTACACTGTGACTGGGGCTGAGCAGATTCAA
                  ************************************************************

HsCD00514516      TACCGAAGGGGCTGGGAAGAAATTATCTCCCTGTGGAACCCCTGGGGCTGGGGCGAGACC
NM_144575.2       TACCGAAGGGGCTGGGAAGAAATTATCTCCCTGTGGAACCCCTGGGGCTGGGGCGAGGCC
                  *********************************************************.**

HsCD00514516      GAATGGAGAGGGCGCTGGAGTGATGGGTCTCAGGAGTGGGAGGAAACCTGTGATCCGCGG
NM_144575.2       GAATGGAGAGGGCGCTGGAGTGATGGGTCTCAGGAGTGGGAGGAAACCTGTGATCCGCGG
                  ************************************************************

HsCD00514516      AAAAGCCAGCTACATAAGAAACGGGAAGATGGCGAGTTTTGGATGTCATGTCAAGATTTC
NM_144575.2       AAAAGCCAGCTACATAAGAAACGGGAAGATGGCGAGTTTTGGATGTCGTGTCAAGATTTC
                  ***********************************************.************

HsCD00514516      CAACAGAAATTCATCGCCATGTTTATATGTAGCGAAATTCCAATTACCCTGGACCATGGA
NM_144575.2       CAACAGAAATTCATCGCCATGTTTATATGTAGCGAAATTCCAATTACCCTGGACCATGGA
                  ************************************************************

HsCD00514516      AACACACTCCACGAAGGATGGTCCCAAATAATGTTTAGGAAGCAAGTGATTCTAGGAAAC
NM_144575.2       AACACACTCCACGAAGGATGGTCCCAAATAATGTTTAGGAAGCAAGTGATTCTAGGAAAC
                  ************************************************************

HsCD00514516      ACTGCAGGAGGACCTCGGAATGATGCTCAATTCAACTTCTCTGTGCAAGAGCCAATGGAA
NM_144575.2       ACTGCAGGAGGACCTCGGAATGATGCTCAATTCAACTTCTCTGTGCAAGAGCCAATGGAA
                  ************************************************************

HsCD00514516      GGCACCAATGTTGTCGTGTGCGTCACAGTTGCTGTCACACCATCAAATTTGAAAGCAGAA
NM_144575.2       GGCACCAATGTTGTCGTGTGCGTCACAGTTGCTGTCACACCATCAAATTTGAAAGCAGAA
                  ************************************************************

HsCD00514516      GATGCAAAATTTCCACTCGATTTCCAAGTGATTCTGGCTGGCTCACAGCGGTTCCGGGAG
NM_144575.2       GATGCAAAATTTCCACTCGATTTCCAAGTGATTCTGGCTGGCTCACAGCGGTTCCGGGAG
                  ************************************************************

HsCD00514516      AAATTTCCACCCGTGTTTTTTTCCTCGTTCAGAAACACTGTCCAAAGCTCAAATAATAAA
NM_144575.2       AAATTTCCACCCGTGTTTTTTTCCTCGTTCAGAAACACTGTCCAAAGCTCAAATAATAAA
                  ************************************************************

HsCD00514516      TTCCGCCGCAACTTCACCATGACTTACCATCTGAGCCCTGGGAACTATGTTGTGGTTGCA
NM_144575.2       TTCCGCCGCAACTTCACCATGACTTACCATCTGAGCCCTGGGAACTATGTTGTGGTTGCA
                  ************************************************************

HsCD00514516      CAGACACGGAGAAAATCAGCGGAGTTCTTGCTCCGAATCTTCCTGAAAATGCCAGACAGT
NM_144575.2       CAGACACGGAGAAAATCAGCGGAGTTCTTGCTCCGAATCTTCCTGAAAATGCCAGACAGT
                  ************************************************************

HsCD00514516      GACAGGCACCTGAGCAGCCATTTCAACCTCAGAATGAAGGGAAGCCCTTCAGAACATGGC
NM_144575.2       GACAGGCACCTGAGCAGCCATTTCAACCTCAGAATGAAGGGAAGCCCTTCAGAACATGGC
                  ************************************************************

HsCD00514516      TCCCAACAAAGCATTTTCAACAGATATGCTCAGCAGAGGCTGGACATTGATGCCACCCAG
NM_144575.2       TCCCAACAAAGCATTTTCAACAGATATGCTCAGCAGAGGCTGGACATTGATGCCACCCAG
                  ************************************************************

HsCD00514516      CTTCAGGGCCTTCTCAACCAGGAGCTTCTAACAGGACCTCCAGGGGACATGTTCTCCTTA
NM_144575.2       CTTCAGGGCCTTCTCAACCAGGAGCTTCTAACAGGACCTCCAGGGGACATGTTCTCCTTA
                  ************************************************************

HsCD00514516      GATGAGTGCCGCAGCTTGGTGGCTCTGATGGAACTGAAAGTGAATGGGCGGCTAGACCAA
NM_144575.2       GATGAGTGCCGCAGCTTGGTGGCTCTGATGGAACTGAAAGTGAATGGGCGGCTAGACCAA
                  ************************************************************

HsCD00514516      GAGGAGTTTGCGCGACTGTGGAAGCGCCTTGTTCACTACCAGCATGTTTTCCAGAAGGTT
NM_144575.2       GAGGAGTTTGCGCGACTGTGGAAGCGCCTTGTTCACTACCAGCATGTTTTCCAGAAGGTT
                  ************************************************************

HsCD00514516      CAGACAAGCCCTGGAGTCCTCCTGAGCTCGGACTTGTGGAAGGCCATAGAGAATACAGAC
NM_144575.2       CAGACAAGCCCTGGAGTCCTCCTGAGCTCGGACTTGTGGAAGGCCATAGAGAATACAGAC
                  ************************************************************

HsCD00514516      TTCCTCAGAGGGATCTTCATCAGCCGTGAGCTGCTGCATCTGGTGACCCTCAGGTACAGC
NM_144575.2       TTCCTCAGAGGGATCTTCATCAGCCGTGAGCTGCTGCATCTGGTGACCCTCAGGTACAGC
                  ************************************************************

HsCD00514516      GACAGCGTCGGCAGGGTCAGCTTCCCCAGCCTGGTCTGCTTCCTGATGCGGCTTGAAGCC
NM_144575.2       GACAGCGTCGGCAGGGTCAGCTTCCCCAGCCTGGTCTGCTTCCTGATGCGGCTTGAAGCC
                  ************************************************************

HsCD00514516      ATGGCAAAGACCTTCCGCAACCTCTCTAAGGATGGAAAAGGACTCTACCTGACAGAAATG
NM_144575.2       ATGGCAAAGACCTTCCGCAACCTCTCTAAGGATGGAAAAGGACTCTACCTGACAGAAATG
                  ************************************************************

HsCD00514516      GAGTGGATGAGCCTGGTCATGTACAAC---
NM_144575.2       GAGTGGATGAGCCTGGTCATGTACAACTGA
                  ***************************   

Protein

SMART domain : CysPc : Calpain-like thiol protease family.
HPRD : 07008

Clone

Cloning Information : 605898
Original Clone ID : ccsbBroadEn_09334

Organism


TAX_ID : 9606
Species Specific ID: 92291

Genome

Chromosome : 2
Map Location : 2p22-p21
Ensembl : ENSG00000162949

Reagents

Labome : CAPN13-antibody