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CAPN11 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN11
Gene Name:  calpain 11
Sequence             
Original Clone ID: ccsbBroadEn_11600
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC033733.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00514558

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2107nts         Open reading frame : 1 to 2107
Reference Sequence Alignment

ATGGTGGCTCACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAAC
GCCCAGAACTTTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGG
GAGCTCTTCGAGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTG
GGCCCCAACTCCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAAC
AACCCTCTATTCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGG
GACTGCTGGCTGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGC
GTGGTGCCCAGAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATT
TGGCAGTTTGGACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGAC
AAGCTGGTGTTTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAG
GCGTATGCCAAGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGC
CTTGAGGACTTCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAAC
CTGCTCAGGCTCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAA
GTCACCAGTGATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCT
TACTCTGTGACTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGG
GTCCGGAATCCCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAG
TGGGAAGAGGTGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAG
TTCTGGATGTCCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTC
ACGCCTGATACACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGC
AGCTGGCGCAGAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACC
AACCCCCAGTTTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGC
AATGTTGTGGCCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGG
CAGCAGGGAGCCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTT
CAGAACATTCAGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGC
TTCTCAGAGATCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGG
GAATATATCATTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGG
GTCTTCACCGAGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAA
CTCCAAGAGGAAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAG
ATAGTGGCAGGAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGG
ATGGCCATCAAATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGC
ATGATCAACCTCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATC
CTGTGGAAAAAACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCA
GGCACCTTGAACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAAC
AACAAGGTAATGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTT
GACAGCTTCATCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATG
GACCCCAAGAATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATG
TGGGGA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN11
Symbol Nomenclature : CAPN11
SYNONYM : calpain11
Designation : CANP 11|calcium-activated neutral proteinase 11|calpain-11
Full Nomenclature : calpain 11
GENEID : 11131
GenBank Accession : JF432283
HGNC : 1478
MIM : 604822
Vega : OTTHUMG00000014758
Target GenBank: JF432283

 

Reference Sequence Alignment

  

HsCD00514558      ------------------------------------------------------------
NM_007058.3       ATGCTGTACTCCCCAGGGCCGAGTCTTCCGGAGTCAGCAGAGAGCCTGGATGGATCACAG
                                                                              

HsCD00514558      ---------------------------------------------------ATGGTGGCT
NM_007058.3       GAGGATAAGCCTCGGGGCTCATGTGCGGAGCCCACTTTTACTGATACGGGAATGGTGGCT
                                                                     *********

HsCD00514558      CACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAACGCCCAGAAC
NM_007058.3       CACATAAACAACAGCCGGCTCAAGGCCAAGGGCGTGGGCCAGCACGACAACGCCCAGAAC
                  ************************************************************

HsCD00514558      TTTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGGGAGCTCTTC
NM_007058.3       TTTGGTAACCAGAGCTTTGAGGAGCTGCGAGCAGCCTGTCTAAGAAAGGGGGAGCTCTTC
                  ************************************************************

HsCD00514558      GAGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTGGGCCCCAAC
NM_007058.3       GAGGACCCCTTATTCCCTGCTGAACCCAGCTCACTGGGCTTCAAGGACCTGGGCCCCAAC
                  ************************************************************

HsCD00514558      TCCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAACAACCCTCTA
NM_007058.3       TCCAAAAATGTGCAGAACATCTCCTGGCAGCGGCCCAAGGATATCATAAACAACCCTCTA
                  ************************************************************

HsCD00514558      TTCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGGGACTGCTGG
NM_007058.3       TTCATCATGGATGGGATTTCTCCAACAGACATCTGCCAGGGGATCCTCGGGGACTGCTGG
                  ************************************************************

HsCD00514558      CTGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGCGTGGTGCCC
NM_007058.3       CTGCTGGCTGCCATCGGCTCCCTTACCACCTGCCCCAAACTGCTATACCGCGTGGTGCCC
                  ************************************************************

HsCD00514558      AGAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATTTGGCAGTTT
NM_007058.3       AGAGGACAGAGCTTCAAGAAAAACTATGCTGGCATCTTCCATTTTCAGATTTGGCAGTTT
                  ************************************************************

HsCD00514558      GGACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGACAAGCTGGTG
NM_007058.3       GGACAGTGGGTGAACGTGGTGGTAGATGACCGGCTGCCCACAAAGAATGACAAGCTGGTG
                  ************************************************************

HsCD00514558      TTTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAGGCGTATGCC
NM_007058.3       TTTGTGCACTCAACCGAACGCAGTGAGTTCTGGAGTGCCCTGCTGGAGAAGGCGTATGCC
                  ************************************************************

HsCD00514558      AAGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGCCTTGAGGAC
NM_007058.3       AAGCTGAGTGGGTCCTATGAAGCATTGTCAGGGGGCAGTACCATGGAGGGCCTTGAGGAC
                  ************************************************************

HsCD00514558      TTCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAACCTGCTCAGG
NM_007058.3       TTCACAGGAGGCGTGGCCCAGAGCTTCCAACTCCAGAGGCCCCCTCAGAACCTGCTCAGG
                  ************************************************************

HsCD00514558      CTCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAAGTCACCAGT
NM_007058.3       CTCCTTAGGAAGGCCGTGGAGCGATCCTCCCTCATGGGTTGCTCCATTGAAGTCACCAGT
                  ************************************************************

HsCD00514558      GATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCTTACTCTGTG
NM_007058.3       GATAGTGAACTGGAATCCATGACTGACAAGATGCTGGTGAGAGGGCACGCTTACTCTGTG
                  ************************************************************

HsCD00514558      ACTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGGGTCCGGAAT
NM_007058.3       ACTGGCCTTCAGGATGTCCACTACAGAGGCAAAATGGAAACACTGATTCGGGTCCGGAAT
                  ************************************************************

HsCD00514558      CCCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAGTGGGAAGAG
NM_007058.3       CCCTGGGGCCGGATTGAGTGGAATGGAGCTTGGAGTGACAGTGCCAGGGAGTGGGAAGAG
                  ************************************************************

HsCD00514558      GTGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAGTTCTGGATG
NM_007058.3       GTGGCCTCAGACATCCAGATGCAGCTGCTGCACAAGACGGAGGACGGGGAGTTCTGGATG
                  ************************************************************

HsCD00514558      TCCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTCACGCCTGAT
NM_007058.3       TCCTACCAAGATTTCCTGAACAACTTCACGCTCCTGGAGATCTGCAACCTCACGCCTGAT
                  ************************************************************

HsCD00514558      ACACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGCAGCTGGCGC
NM_007058.3       ACACTCTCTGGGGACTACAAGAGCTACTGGCACACCACCTTCTACGAGGGCAGCTGGCGC
                  ************************************************************

HsCD00514558      AGAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACCAACCCCCAG
NM_007058.3       AGAGGCAGCTCCGCAGGGGGCTGCAGGAACCACCCTGGCACGTTCTGGACCAACCCCCAG
                  ************************************************************

HsCD00514558      TTTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGCAATGTTGTG
NM_007058.3       TTTAAGATCTCTCTTCCTGAGGGGGATGACCCAGAGGATGACGCAGAGGGCAATGTTGTG
                  ************************************************************

HsCD00514558      GCCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGGCAGCAGGGA
NM_007058.3       GTCTGCACCTGCCTGGTGGCCCTAATGCAGAAGAACTGGCGGCATGCACGGCAGCAGGGA
                  * **********************************************************

HsCD00514558      GCCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTTCAGAACATT
NM_007058.3       GCCCAGCTGCAGACCATTGGCTTTGTCCTCTACGCGGTCCCAAAAGAGTTTCAGAACATT
                  ************************************************************

HsCD00514558      CAGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGCTTCTCAGAG
NM_007058.3       CAGGATGTCCACTTGAAGAAGGAATTCTTCACGAAGTATCAGGACCACGGCTTCTCAGAG
                  ************************************************************

HsCD00514558      ATCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGGGAATATATC
NM_007058.3       ATCTTCACCAACTCACGGGAGGTGAGCAGCCAACTCCGGCTGCCTCCGGGGGAATATATC
                  ************************************************************

HsCD00514558      ATTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGGGTCTTCACC
NM_007058.3       ATTATTCCCTCCACCTTTGAGCCACACAGAGATGCTGACTTCCTGCTTCGGGTCTTCACC
                  ************************************************************

HsCD00514558      GAGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAACTCCAAGAG
NM_007058.3       GAGAAGCACAGCGAGTCATGGGAATTGGATGAAGTCAACTATGCTGAGCAACTCCAAGAG
                  ************************************************************

HsCD00514558      GAAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAGATAGTGGCA
NM_007058.3       GAAAAGGTCTCTGAGGATGACATGGACCAGGACTTCCTACATTTGTTTAAGATAGTGGCA
                  ************************************************************

HsCD00514558      GGAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGGATGGCCATC
NM_007058.3       GGAGAGGGCAAGGAGATAGGGGTGTATGAGCTCCAGAGGCTGCTCAACAGGATGGCCATC
                  ************************************************************

HsCD00514558      AAATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGCATGATCAAC
NM_007058.3       AAATTCAAAAGCTTCAAGACCAAGGGCTTTGGCCTGGATGCTTGCCGCTGCATGATCAAC
                  ************************************************************

HsCD00514558      CTCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATCCTGTGGAAA
NM_007058.3       CTCATGGATAAAGATGGCTCTGGCAAGCTGGGGCTTCTAGAGTTCAAGATCCTGTGGAAA
                  ************************************************************

HsCD00514558      AAACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCAGGCACCTTG
NM_007058.3       AAACTCAAGAAATGGATGGACATCTTCAGAGAGTGTGACCAGGACCATTCAGGCACCTTG
                  ************************************************************

HsCD00514558      AACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAACAACAAGGTA
NM_007058.3       AACTCCTATGAGATGCGCCTGGTTATTGAGAAAGCAGGCATCAAGCTGAACAACAAGGTA
                  ************************************************************

HsCD00514558      ATGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTTGACAGCTTC
NM_007058.3       ATGCAGGTCCTGGTGGCCAGGTATGCAGATGATGACCTGATCATAGACTTTGACAGCTTC
                  ************************************************************

HsCD00514558      ATCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATGGACCCCAAG
NM_007058.3       ATCAGCTGTTTCCTGAGGCTAAAGACCATGTTCACATTCTTTCTAACCATGGACCCCAAG
                  ************************************************************

HsCD00514558      AATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATGTGGGGA---
NM_007058.3       AATACTGGCCATATTTGCTTGAGCCTGGAACAGTGGCTGCAGATGACCATGTGGGGATAG
                  *********************************************************   

Pathways

Panther : Huntington disease
WikiPathway : Integrin-mediated cell adhesion

Protein

SMART domain : CysPc : Calpain-like thiol protease family.
SMART domain : calpain_III :
HPRD : 05315

Clone

Cloning Information : 605702
Original Clone ID : ccsbBroadEn_11600

Organism


TAX_ID : 9606
Species Specific ID: 11131

Genome

Chromosome : 6
Map Location : 6p12
Ensembl : ENSG00000137225

Reagents

Labome : CAPN11-antibody