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FGF11 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FGF11
Gene Name:  fibroblast growth factor 11
Sequence             
Original Clone ID: ccsbBroadEn_00558
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC032502.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00515483

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 676nts         Open reading frame : 1 to 676
Reference Sequence Alignment

ATGGCGGCGCTGGCCAGTAGCCTGATCCGGCAGAAGCGGGAGGTCCGCGAGCCCGGGGGC
AGCCGGCCGGTGTCGGCGCAGCGGCGCGTGTGTCCCCGCGGCACCAAGTCCCTTTGCCAG
AAGCAGCTCCTCATCCTGCTGTCCAAGGTGCGACTGTGCGGGGGGCGGCCCGCGCGGCCG
GACCGCGGCCCGGAGCCTCAGCTCAAAGGCATCGTCACCAAACTGTTCTGCCGCCAGGGT
TTCTACCTCCAGGCGAATCCCGACGGAAGCATCCAGGGCACCCCAGAGGATACCAGCTCC
TTCACCCACTTCAACCTGATCCCTGTGGGCCTCCGTGTGGTCACCATCCAGAGCGCCAAG
CTGGGTCACTACATGGCCATGAATGCTGAGGGACTGCTCTACAGTTCGCCGCATTTCACA
GCTGAGTGTCGCTTTAAGGAGTGTGTCTTTGAGAATTACTACGTCCTGTACGCCTCTGCT
CTCTACCGCCAGCGTCGTTCTGGCCGGGCCTGGTACCTCGGCCTGGACAAGGAGGGCCAG
GTCATGAAGGGAAACCGAGTTAAGAAGACCAAGGCAGCTGCCCACTTTCTGCCCAAGCTC
CTGGAGGTGGCCATGTACCAGGAGCCTTCTCTCCACAGTGTCCCCGAGGCCTCCCCTTCC
AGTCCCCCTGCCCCC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FGF11
Symbol Nomenclature : FGF11
SYNONYM : FHF3
Designation : FGF-11|FHF-3|fibroblast growth factor homologous factor 3
Full Nomenclature : fibroblast growth factor 11
GENEID : 2256
HGNC : 3667
MIM : 601514
Vega : OTTHUMG00000108136
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00515483      ATGGCGGCGCTGGCCAGTAGCCTGATCCGGCAGAAGCGGGAGGTCCGCGAGCCCGGGGGC
NM_004112.2       ATGGCGGCGCTGGCCAGTAGCCTGATCCGGCAGAAGCGGGAGGTCCGCGAGCCCGGGGGC
                  ************************************************************

HsCD00515483      AGCCGGCCGGTGTCGGCGCAGCGGCGCGTGTGTCCCCGCGGCACCAAGTCCCTTTGCCAG
NM_004112.2       AGCCGGCCGGTGTCGGCGCAGCGGCGCGTGTGTCCCCGCGGCACCAAGTCCCTTTGCCAG
                  ************************************************************

HsCD00515483      AAGCAGCTCCTCATCCTGCTGTCCAAGGTGCGACTGTGCGGGGGGCGGCCCGCGCGGCCG
NM_004112.2       AAGCAGCTCCTCATCCTGCTGTCCAAGGTGCGACTGTGCGGGGGGCGGCCCGCGCGGCCG
                  ************************************************************

HsCD00515483      GACCGCGGCCCGGAGCCTCAGCTCAAAGGCATCGTCACCAAACTGTTCTGCCGCCAGGGT
NM_004112.2       GACCGCGGCCCGGAGCCTCAGCTCAAAGGCATCGTCACCAAACTGTTCTGCCGCCAGGGT
                  ************************************************************

HsCD00515483      TTCTACCTCCAGGCGAATCCCGACGGAAGCATCCAGGGCACCCCAGAGGATACCAGCTCC
NM_004112.2       TTCTACCTCCAGGCGAATCCCGACGGAAGCATCCAGGGCACCCCAGAGGATACCAGCTCC
                  ************************************************************

HsCD00515483      TTCACCCACTTCAACCTGATCCCTGTGGGCCTCCGTGTGGTCACCATCCAGAGCGCCAAG
NM_004112.2       TTCACCCACTTCAACCTGATCCCTGTGGGCCTCCGTGTGGTCACCATCCAGAGCGCCAAG
                  ************************************************************

HsCD00515483      CTGGGTCACTACATGGCCATGAATGCTGAGGGACTGCTCTACAGTTCGCCGCATTTCACA
NM_004112.2       CTGGGTCACTACATGGCCATGAATGCTGAGGGACTGCTCTACAGTTCGCCGCATTTCACA
                  ************************************************************

HsCD00515483      GCTGAGTGTCGCTTTAAGGAGTGTGTCTTTGAGAATTACTACGTCCTGTACGCCTCTGCT
NM_004112.2       GCTGAGTGTCGCTTTAAGGAGTGTGTCTTTGAGAATTACTACGTCCTGTACGCCTCTGCT
                  ************************************************************

HsCD00515483      CTCTACCGCCAGCGTCGTTCTGGCCGGGCCTGGTACCTCGGCCTGGACAAGGAGGGCCAG
NM_004112.2       CTCTACCGCCAGCGTCGTTCTGGCCGGGCCTGGTACCTCGGCCTGGACAAGGAGGGCCAG
                  ************************************************************

HsCD00515483      GTCATGAAGGGAAACCGAGTTAAGAAGACCAAGGCAGCTGCCCACTTTCTGCCCAAGCTC
NM_004112.2       GTCATGAAGGGAAACCGAGTTAAGAAGACCAAGGCAGCTGCCCACTTTCTGCCCAAGCTC
                  ************************************************************

HsCD00515483      CTGGAGGTGGCCATGTACCAGGAGCCTTCTCTCCACAGTGTCCCCGAGGCCTCCCCTTCC
NM_004112.2       CTGGAGGTGGCCATGTACCAGGAGCCTTCTCTCCACAGTGTCCCCGAGGCCTCCCCTTCC
                  ************************************************************

HsCD00515483      AGTCCCCCTGCCCCC---
NM_004112.2       AGTCCCCCTGCCCCCTGA
                  ***************   

Pathways

Panther : FGF signaling pathway
WikiPathway : Regulation of Actin Cytoskeleton

Clone

Cloning Information : 665848
Original Clone ID : ccsbBroadEn_00558

Organism


TAX_ID : 9606
Species Specific ID: 2256

Genome

Chromosome : 17
Map Location : 17p13.1
Ensembl : ENSG00000161958

Reagents

Labome : FGF11-antibody