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ACTBL2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ACTBL2
Gene Name:  actin, beta-like 2
Sequence             
Original Clone ID: ccsbBroadEn_05497
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC137470.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00515605

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1129nts        
Open reading frame : 1 to 1129
Reference Sequence Alignment

ATGACTGACAATGAGCTGTCTGCCTTGGTAGTGGATAATGGGTCAGGGATGTGCAAGGCA
GGCTTTGGTGGTGACGATGCCCCCCGGGCTGTGTTCCCCTCCATGATAGGGCGTCCTCGA
CACCAGGGCGTTATGGTAGGCATGGGCCAGAAGGACTGCTACGTGGGAGATGAGGCTCAG
AGCAAGAGAGGCGTCCTGACCCTGAAGTATCCTATCGAGCATGGAGTGGTCACCAACTGG
GACGATATGGAAAAGATCTGGTACCACACATTCTACAATGAGCTCCGTGTGGCACCAGAT
GAGCATCCCATCCTCCTCACCGAGGCACCCCTGAACCCCAAGATCAACCGGGAAAAGATG
ACTCAGATCATGTTCGAGGCTTTCAACACACCAGCCATGTATGTCGCCATCCAGGCTGTG
CTGTCCCTCTATGCCTCAGGACGGACCACAGGCATCGTGATGGATTCTGGGGATGGGGTC
ACTCACATCGTGCCCATCTATGAAGGTTATGCCCTGCCTCATGCCATTCTACGCCTGGAT
CTGGCAGGGAGAGACCTGACTGATTACCTCATGAAGATCCTGACAGAGCGAGGCTATAAC
TTCACCACCACTGCTGAGCGGGAGATTGTGCGAGATGTCAAAGAGAAGCTGTGCTACGTA
GCCCTGGACTTTGAGCAGGAGATGGTCAGGGCAGCCGCATCCTCCTCACCGGAACGGAGC
TATGAACTTCCTGATGGGCAGGTGATCACCATTGGGAATGAACGCTTCCGATGCCCTGAA
GCCATTTTCCAGCCTTCCTTTCTGGGCATTGAGTCCAGTGGGATCCATGAGACAACCTTC
AACTCTATCATGAAGTGCGATGTGGATATTCGCAAGGATCTCTATGCCAACACCGTGTTA
TCTGGAGGGAGCACCATGTACCCAGGCATTGCTGACAGGATGCAGAAAGAAATCATAACC
CTGGCACCCAGCACCATGAAAATCAAGATCATAGCTCCCCCAGAGCGGAAGTATTCTGTT
TGGATTGGGGGCTCCATCCTTGCCAGCCTGTCCACATTCCAGCAGATGTGGATCAGTAAG
CAGGAATATGATGAGGCTGGTCCTCCTATCGTTCACAGAAAATGTTTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ACTBL2
Symbol Nomenclature : ACTBL2
SYNONYM : ACT
Designation : actin-like protein|beta-actin-like protein 2|kappa-actin
Full Nomenclature : actin, beta-like 2
GENEID : 345651
HGNC : 17780
MIM : 614835
Vega : OTTHUMG00000162330
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00515605        ATGACTGACAATGAGCTGTCTGCCTTGGTAGTGGATAATGGGTCAGGGATGTGCAAGGCA
NM_001199954.1      ---ATGGAAGAAGAGATCGCCGCGCTGGTCATTGACAATGGCTCCGGCATGTGCAAAGCT
NM_001101.3         ---ATGGATGATGATATCGCCGCGCTCGTCGTCGACAACGGCTCCGGCATGTGCAAGGCC
NM_001614.3         ---ATGGAAGAAGAGATCGCCGCGCTGGTCATTGACAATGGCTCCGGCATGTGCAAAGCT
NM_001017992.3      ATGACTGACAATGAGCTGTCTGCCTTGGTAGTGGATAATGGGTCAGGGATGTGCAAGGCA
                       *  ** .*:** .*  * **  * **..* ** ** ** **.** ********.** 

HsCD00515605        GGCTTTGGTGGTGACGATGCCCCCCGGGCTGTGTTCCCCTCCATGATAGGGCGTCCTCGA
NM_001199954.1      GGTTTTGCTGGGGACGACGCTCCCCGAGCCGTGTTTCCTTCCATCGTCGGGCGCCCCAGA
NM_001101.3         GGCTTCGCGGGCGACGATGCCCCCCGGGCCGTCTTCCCCTCCATCGTGGGGCGCCCCAGG
NM_001614.3         GGTTTTGCTGGGGACGACGCTCCCCGAGCCGTGTTTCCTTCCATCGTCGGGCGCCCCAGA
NM_001017992.3      GGCTTTGGTGGTGACGATGCCCCCCGGGCTGTGTTCCCCTCCATGATAGGGCGTCCTCGA
                    ** ** *  ** ***** ** *****.** ** ** ** ***** .* ***** ** .*.

HsCD00515605        CACCAGGGCGTTATGGTAGGCATGGGCCAGAAGGACTGCTACGTGGGAGATGAGGCTCAG
NM_001199954.1      CACCAGGGCGTCATGGTGGGCATGGGCCAGAAGGACTCCTACGTGGGCGACGAGGCCCAG
NM_001101.3         CACCAGGGCGTGATGGTGGGCATGGGTCAGAAGGATTCCTATGTGGGCGACGAGGCCCAG
NM_001614.3         CACCAGGGCGTCATGGTGGGCATGGGCCAGAAGGACTCCTACGTGGGCGACGAGGCCCAG
NM_001017992.3      CACCAGGGCGTTATGGTAGGCATGGGCCAGAAGGACTGCTACGTGGGAGATGAGGCTCAG
                    *********** *****.******** ******** * *** *****.** ***** ***

HsCD00515605        AGCAAGAGAGGCGTCCTGACCCTGAAGTATCCTATCGAGCATGGAGTGGTCACCAACTGG
NM_001199954.1      AGCAAGCGTGGCATCCTGACCCTGAAGTACCCCATTGAGCATGGCATCGTCACCAACTGG
NM_001101.3         AGCAAGAGAGGCATCCTCACCCTGAAGTACCCCATCGAGCACGGCATCGTCACCAACTGG
NM_001614.3         AGCAAGCGTGGCATCCTGACCCTGAAGTACCCCATTGAGCATGGCATCGTCACCAACTGG
NM_001017992.3      AGCAAGAGAGGCGTCCTGACCCTGAAGTATCCTATCGAGCATGGAGTGGTCACCAACTGG
                    ******.*:***.**** *********** ** ** ***** **..* ************

HsCD00515605        GACGATATGGAAAAGATCTGGTACCACACATTCTACAATGAGCTCCGTGTGGCACCAGAT
NM_001199954.1      GACGACATGGAGAAGATCTGGCACCACACCTTCTACAACGAGCTGCGCGTGGCCCCGGAG
NM_001101.3         GACGACATGGAGAAAATCTGGCACCACACCTTCTACAATGAGCTGCGTGTGGCTCCCGAG
NM_001614.3         GACGACATGGAGAAGATCTGGCACCACACCTTCTACAACGAGCTGCGCGTGGCCCCGGAG
NM_001017992.3      GACGATATGGAAAAGATCTGGTACCACACATTCTACAATGAGCTCCGTGTGGCACCAGAT
                    ***** *****.**.****** *******.******** ***** ** ***** ** ** 

HsCD00515605        GAGCATCCCATCCTCCTCACCGAGGCACCCCTGAACCCCAAGATCAACCGGGAAAAGATG
NM_001199954.1      GAGCACCCAGTGCTGCTGACCGAGGCCCCCCTGAACCCCAAGGCCAACAGAGAGAAGATG
NM_001101.3         GAGCACCCCGTGCTGCTGACCGAGGCCCCCCTGAACCCCAAGGCCAACCGCGAGAAGATG
NM_001614.3         GAGCACCCAGTGCTGCTGACCGAGGCCCCCCTGAACCCCAAGGCCAACAGAGAGAAGATG
NM_001017992.3      GAGCATCCCATCCTCCTCACCGAGGCACCCCTGAACCCCAAGATCAACCGGGAAAAGATG
                    ***** **..* ** ** ********.***************. ****.* **.******

HsCD00515605        ACTCAGATCATGTTCGAGGCTTTCAACACACCAGCCATGTATGTCGCCATCCAGGCTGTG
NM_001199954.1      ACTCAGATTATGTTTGAGACCTTCAACACCCCGGCCATGTACGTGGCCATCCAGGCCGTG
NM_001101.3         ACCCAGATCATGTTTGAGACCTTCAACACCCCAGCCATGTACGTTGCTATCCAGGCTGTG
NM_001614.3         ACTCAGATTATGTTTGAGACCTTCAACACCCCGGCCATGTACGTGGCCATCCAGGCCGTG
NM_001017992.3      ACTCAGATCATGTTCGAGGCTTTCAACACACCAGCCATGTATGTCGCCATCCAGGCTGTG
                    ** ***** ***** ***.* ********.**.******** ** ** ******** ***

HsCD00515605        CTGTCCCTCTATGCCTCAGGACGGACCACAGGCATCGTGATGGATTCTGGGGATGGGGTC
NM_001199954.1      CTGTCCCTCTACGCCTCTGGGCGCACCACTGGCATTGTCATGGACTCTGGAGACGGGGTC
NM_001101.3         CTATCCCTGTACGCCTCTGGCCGTACCACTGGCATCGTGATGGACTCCGGTGACGGGGTC
NM_001614.3         CTGTCCCTCTACGCCTCTGGGCGCACCACTGGCATTGTCATGGACTCTGGAGACGGGGTC
NM_001017992.3      CTGTCCCTCTATGCCTCAGGACGGACCACAGGCATCGTGATGGATTCTGGGGATGGGGTC
                    **.***** ** *****:** ** *****:***** ** ***** ** ** ** ******

HsCD00515605        ACTCACATCGTGCCCATCTATGAAGGTTATGCCCTGCCTCATGCCATTCTACGCCTGGAT
NM_001199954.1      ACCCACACGGTGCCCATCTACGAGGGCTACGCCCTCCCCCACGCCATCCTGCGTCTGGAC
NM_001101.3         ACCCACACTGTGCCCATCTACGAGGGGTATGCCCTCCCCCATGCCATCCTGCGTCTGGAC
NM_001614.3         ACCCACACGGTGCCCATCTACGAGGGCTACGCCCTCCCCCACGCCATCCTGCGTCTGGAC
NM_001017992.3      ACTCACATCGTGCCCATCTATGAAGGTTATGCCCTGCCTCATGCCATTCTACGCCTGGAT
                    ** ****  *********** **.** ** ***** ** ** ***** **.** ***** 

HsCD00515605        CTGGCAGGGAGAGACCTGACTGATTACCTCATGAAGATCCTGACAGAGCGAGGCTATAAC
NM_001199954.1      CTGGCTGGCCGGGACCTGACCGACTACCTCATGAAGATCCTCACTGAGCGAGGCTACAGC
NM_001101.3         CTGGCTGGCCGGGACCTGACTGACTACCTCATGAAGATCCTCACCGAGCGCGGCTACAGC
NM_001614.3         CTGGCTGGCCGGGACCTGACCGACTACCTCATGAAGATCCTCACTGAGCGAGGCTACAGC
NM_001017992.3      CTGGCAGGGAGAGACCTGACTGATTACCTCATGAAGATCCTGACAGAGCGAGGCTATAAC
                    *****:** .*.******** ** ***************** ** *****.***** *.*

HsCD00515605        TTCACCACCACTGCTGAGCGGGAGATTGTGCGAGATGTCAAAGAGAAGCTGTGCTACGTA
NM_001199954.1      TTCACCACCACGGCCGAGCGGGAAATCGTGCGCGACATCAAGGAGAAGCTGTGCTACGTC
NM_001101.3         TTCACCACCACGGCCGAGCGGGAAATCGTGCGTGACATTAAGGAGAAGCTGTGCTACGTC
NM_001614.3         TTCACCACCACGGCCGAGCGGGAAATCGTGCGCGACATCAAGGAGAAGCTGTGCTACGTC
NM_001017992.3      TTCACCACCACTGCTGAGCGGGAGATTGTGCGAGATGTCAAAGAGAAGCTGTGCTACGTA
                    *********** ** ********.** ***** ** .* **.*****************.

HsCD00515605        GCCCTGGACTTTGAGCAGGAGATGGTCAGGGCAGCCGCATCCTCCTCACCGGAACGGAGC
NM_001199954.1      GCCCTGGACTTCGAGCAGGAGATGGCCACCGCCGCATCCTCCTCTTCTCTGGAGAAGAGC
NM_001101.3         GCCCTGGACTTCGAGCAAGAGATGGCCACGGCTGCTTCCAGCTCCTCCCTGGAGAAGAGC
NM_001614.3         GCCCTGGACTTCGAGCAGGAGATGGCCACCGCCGCATCCTCCTCTTCTCTGGAGAAGAGC
NM_001017992.3      GCCCTGGACTTTGAGCAGGAGATGGTCAGGGCAGCCGCATCCTCCTCACCGGAACGGAGC
                    *********** *****.******* **  ** **  *.: *** ** * ***...****

HsCD00515605        TATGAACTTCCTGATGGGCAGGTGATCACCATTGGGAATGAACGCTTCCGATGCCCTGAA
NM_001199954.1      TACGAGCTGCCCGATGGCCAGGTCATCACCATTGGCAATGAGCGGTTCCGGTGTCCGGAG
NM_001101.3         TACGAGCTGCCTGACGGCCAGGTCATCACCATTGGCAATGAGCGGTTCCGCTGCCCTGAG
NM_001614.3         TACGAGCTGCCCGATGGCCAGGTCATCACCATTGGCAATGAGCGGTTCCGGTGTCCGGAG
NM_001017992.3      TATGAACTTCCTGATGGGCAGGTGATCACCATTGGGAATGAACGCTTCCGATGCCCTGAA
                    ** **.** ** ** ** ***** *********** *****.** ***** ** ** **.

HsCD00515605        GCCATTTTCCAGCCTTCCTTTCTGGGCATTGAGTCCAGTGGGATCCATGAGACAACCTTC
NM_001199954.1      GCGCTGTTCCAGCCTTCCTTCCTGGGTATGGAATCTTGCGGCATCCACGAGACCACCTTC
NM_001101.3         GCACTCTTCCAGCCTTCCTTCCTGGGCATGGAGTCCTGTGGCATCCACGAAACTACCTTC
NM_001614.3         GCGCTGTTCCAGCCTTCCTTCCTGGGTATGGAATCTTGCGGCATCCACGAGACCACCTTC
NM_001017992.3      GCCATTTTCCAGCCTTCCTTTCTGGGCATTGAGTCCAGTGGGATCCATGAGACAACCTTC
                    ** .* ************** ***** ** **.** :* ** ***** **.** ******

HsCD00515605        AACTCTATCATGAAGTGCGATGTGGATATTCGCAAGGATCTCTATGCCAACACCGTGTTA
NM_001199954.1      AACTCCATCATGAAGTGTGACGTGGACATCCGCAAAGACCTGTACGCCAACACGGTGCTG
NM_001101.3         AACTCCATCATGAAGTGTGACGTGGACATCCGCAAAGACCTGTACGCCAACACAGTGCTG
NM_001614.3         AACTCCATCATGAAGTGTGACGTGGACATCCGCAAAGACCTGTACGCCAACACGGTGCTG
NM_001017992.3      AACTCTATCATGAAGTGCGATGTGGATATTCGCAAGGATCTCTATGCCAACACCGTGTTA
                    ***** *********** ** ***** ** *****.** ** ** ******** *** *.

HsCD00515605        TCTGGAGGGAGCACCATGTACCCAGGCATTGCTGACAGGATGCAGAAAGAAATCATAACC
NM_001199954.1      TCGGGCGGCACCACCATGTACCCGGGCATTGCCGACAGGATGCAGAAGGAGATCACCGCC
NM_001101.3         TCTGGCGGCACCACCATGTACCCTGGCATTGCCGACAGGATGCAGAAGGAGATCACTGCC
NM_001614.3         TCGGGCGGCACCACCATGTACCCGGGCATTGCCGACAGGATGCAGAAGGAGATCACCGCC
NM_001017992.3      TCTGGAGGGAGCACCATGTACCCAGGCATTGCTGACAGGATGCAGAAAGAAATCATAACC
                    ** **.** * ************ ******** **************.**.****  .**

HsCD00515605        CTGGCACCCAGCACCATGAAAATCAAGATCATAGCTCCCCCAGAGCGGAAGTATTCTGTT
NM_001199954.1      CTGGCGCCCAGCACCATGAAGATCAAGATCATCGCACCCCCAGAGCGCAAGTACTCGGTG
NM_001101.3         CTGGCACCCAGCACAATGAAGATCAAGATCATTGCTCCTCCTGAGCGCAAGTACTCCGTG
NM_001614.3         CTGGCGCCCAGCACCATGAAGATCAAGATCATCGCACCCCCAGAGCGCAAGTACTCGGTG
NM_001017992.3      CTGGCACCCAGCACCATGAAAATCAAGATCATAGCTCCCCCAGAGCGGAAGTATTCTGTT
                    *****.********.*****.*********** **:** **:***** ***** ** ** 

HsCD00515605        TGGATTGGGGGCTCCATCCTTGCCAGCCTGTCCACATTCCAGCAGATGTGGATCAGTAAG
NM_001199954.1      TGGATCGGTGGCTCCATCCTGGCCTCACTGTCCACCTTCCAGCAGATGTGGATTAGCAAG
NM_001101.3         TGGATCGGCGGCTCCATCCTGGCCTCGCTGTCCACCTTCCAGCAGATGTGGATCAGCAAG
NM_001614.3         TGGATCGGTGGCTCCATCCTGGCCTCACTGTCCACCTTCCAGCAGATGTGGATTAGCAAG
NM_001017992.3      TGGATTGGGGGCTCCATCCTTGCCAGCCTGTCCACATTCCAGCAGATGTGGATCAGTAAG
                    ***** ** *********** ***:  ********.***************** ** ***

HsCD00515605        CAGGAATATGATGAGGCTGGTCCTCCTATCGTTCACAGAAAATGTTTC---
NM_001199954.1      CAGGAGTACGACGAGTCGGGCCCCTCCATCGTCCACCGCAAATGCTTCTAA
NM_001101.3         CAGGAGTATGACGAGTCCGGCCCCTCCATCGTCCACCGCAAATGCTTCTAG
NM_001614.3         CAGGAGTACGACGAGTCGGGCCCCTCCATCGTCCACCGCAAATGCTTCTAA
NM_001017992.3      CAGGAATATGATGAGGCTGGTCCTCCTATCGTTCACAGAAAATGTTTCTGA
                    *****.** ** *** * ** **  * ***** ***.*.***** ***   

Pathways

Panther : Alzheimer disease-presenilin pathway
Panther : Cadherin signaling pathway
Panther : Cytoskeletal regulation by Rho GTPase
Panther : Huntington disease
Panther : Inflammation mediated by chemokine and cytokine signaling pathway
Panther : Integrin signalling pathway
Panther : Nicotinic acetylcholine receptor signaling pathway

Protein

SMART domain : ACTIN : Actin
HPRD : 18598

Clone

Cloning Information : 668696
Original Clone ID : ccsbBroadEn_05497

Organism


TAX_ID : 9606
Species Specific ID: 345651

Genome

Chromosome : 5
Map Location : 5q11.2
Ensembl : ENSG00000169067

Reagents

Labome : ACTBL2-antibody