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LGALS7B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  LGALS7B
Gene Name:  lectin, galactoside-binding, soluble, 7B
Sequence             
Original Clone ID: ccsbBroadEn_10193
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC061588.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00515762

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 409nts         Open reading frame : 1 to 409
Reference Sequence Alignment

ATGTCCAACGTCCCCCACAAGTCCTCGCTGCCCGAGGGCATCCGCCCTGGCACGGTGCTG
AGAATTCGCGGCTTGGTTCCTCCCAATGCCAGCAGGTTCCATGTAAACCTGCTGTGCGGG
GAGGAGCAGGGCTCCGATGCCGCCCTGCATTTCAACCCCCGGCTGGACACGTCGGAGGTG
GTCTTCAACAGCAAGGAGCAAGGCTCCTGGGGCCGCGAGGAGCGCGGGCCGGGCGTTCCT
TTCCAGCGCGGGCAGCCCTTCGAGGTGCTCATCATCGCGTCAGACGACGGCTTCAAGGCC
GTGGTTGGGGACGCCCAGTACCACCACTTCCGCCACCGCCTGCCGCTGGCGCGCGTGCGC
CTGGTGGAGGTGGGCGGGGACGTGCAGCTGGACTCCGTGAGGATCTTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : LGALS7B
Symbol Nomenclature : LGALS7B
SYNONYM : GAL7; Gal-7; HKL-14; LGALS7; PI7
Designation : galectin 7B|galectin-7|galectin-7B|keratinocyte lectin 14|lectin galactoside-binding soluble 7|p53-induced gene 1 protein|p53-induced protein 1
Full Nomenclature : lectin, galactoside-binding, soluble, 7B
GENEID : 653499
HGNC : 34447
Vega : OTTHUMG00000182607
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00515762        ATGTCCAACGTCCCCCACAAGTCCTCGCTGCCCGAGGGCATCCGCCCTGGCACGGTGCTG
NM_001042507.3      ATGTCCAACGTCCCCCACAAGTCCTCACTGCCCGAGGGCATCCGCCCTGGCACGGTGCTG
NM_002307.3         ATGTCCAACGTCCCCCACAAGTCCTCACTGCCCGAGGGCATCCGCCCTGGCACGGTGCTG
                    **************************.*********************************

HsCD00515762        AGAATTCGCGGCTTGGTTCCTCCCAATGCCAGCAGGTTCCATGTAAACCTGCTGTGCGGG
NM_001042507.3      AGAATTCGCGGCTTGGTTCCTCCCAATGCCAGCAGGTTCCATGTAAACCTGCTGTGCGGG
NM_002307.3         AGAATTCGCGGCTTGGTTCCTCCCAATGCCAGCAGGTTCCATGTAAACCTGCTGTGCGGG
                    ************************************************************

HsCD00515762        GAGGAGCAGGGCTCCGATGCCGCCCTGCATTTCAACCCCCGGCTGGACACGTCGGAGGTG
NM_001042507.3      GAGGAGCAGGGCTCCGATGCCGCCCTGCATTTCAACCCCCGGCTGGACACGTCGGAGGTG
NM_002307.3         GAGGAGCAGGGCTCCGATGCCGCGCTGCATTTCAACCCCCGGCTGGACACGTCGGAGGTG
                    *********************** ************************************

HsCD00515762        GTCTTCAACAGCAAGGAGCAAGGCTCCTGGGGCCGCGAGGAGCGCGGGCCGGGCGTTCCT
NM_001042507.3      GTCTTCAACAGCAAGGAGCAAGGCTCCTGGGGCCGCGAGGAGCGCGGGCCGGGCGTTCCT
NM_002307.3         GTCTTCAACAGCAAGGAGCAAGGCTCCTGGGGCCGCGAGGAGCGCGGGCCGGGCGTTCCT
                    ************************************************************

HsCD00515762        TTCCAGCGCGGGCAGCCCTTCGAGGTGCTCATCATCGCGTCAGACGACGGCTTCAAGGCC
NM_001042507.3      TTCCAGCGCGGGCAGCCCTTCGAGGTGCTCATCATCGCGTCAGACGACGGCTTCAAGGCC
NM_002307.3         TTCCAGCGCGGGCAGCCCTTCGAGGTGCTCATCATCGCGTCAGACGACGGCTTCAAGGCC
                    ************************************************************

HsCD00515762        GTGGTTGGGGACGCCCAGTACCACCACTTCCGCCACCGCCTGCCGCTGGCGCGCGTGCGC
NM_001042507.3      GTGGTTGGGGACGCCCAGTACCACCACTTCCGCCACCGCCTGCCGCTGGCGCGCGTGCGC
NM_002307.3         GTGGTTGGGGACGCCCAGTACCACCACTTCCGCCACCGCCTGCCGCTGGCGCGCGTGCGC
                    ************************************************************

HsCD00515762        CTGGTGGAGGTGGGCGGGGACGTGCAGCTGGACTCCGTGAGGATCTTC---
NM_001042507.3      CTGGTGGAGGTGGGCGGGGACGTGCAGCTGGACTCCGTGAGGATCTTCTGA
NM_002307.3         CTGGTGGAGGTGGGCGGGGACGTGCAGCTGGACTCCGTGAGGATCTTCTGA
                    ************************************************   

Clone

Cloning Information : 667879
Original Clone ID : ccsbBroadEn_10193

Organism


TAX_ID : 9606
Species Specific ID: 653499

Genome

Chromosome : 19
Map Location : 19q13.2
Ensembl : ENSG00000178934

Reagents

Labome : LGALS7B-antibody