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ZDHHC1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZDHHC1
Gene Name:  zinc finger, DHHC-type containing 1
Sequence             
Original Clone ID: ccsbBroadEn_03094
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC021908.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 23844586
Title: Profiling targets of the irreversible palmitoylation inhibitor 2-bromopalmitate.
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00515835

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1456nts         Open reading frame : 1 to 1456
Reference Sequence Alignment

ATGTACAAGATGAACATCTGCAACAAGCCCTCCAACAAGACGGCCCCTGAGAAGAGTGTG
TGGACGGCACCGGCACAGCCCAGCGGACCCTCCCCTGAGCTGCAGGGCCAGCGATCCCGC
CGGAATGGGTGGAGCTGGCCCCCTCACCCGCTCCAGATTGTGGCCTGGCTGCTGTACCTC
TTCTTTGCTGTGATCGGCTTTGGGATCCTTGTTCCCCTCCTGCCTCACCACTGGGTGCCC
GCTGGCTACGCTTGCATGGGCGCCATCTTTGCTGGCCACCTTGTGGTGCACCTGACCGCC
GTCTCCATCGATCCAGCAGATGCCAACGTGCGGGACAAGAGCTATGCGGGGCCCCTGCCC
ATCTTCAACCGAAGCCAGCACGCACATGTCATTGAAGACCTGCACTGCAACTTGTGCAAC
GTGGATGTGAGCGCTCGCTCCAAGCACTGCAGCGCCTGCAACAAGTGCGTGTGCGGTTTC
GACCACCACTGCAAGTGGCTCAACAACTGTGTGGGCGAGCGGAACTACCGGCTCTTTCTA
CACAGTGTTGCATCCGCTTTACTGGGCGTCCTGCTCCTGGTGCTGGTGGCCACATATGTC
TTCGTGGAGTTCTTTGTCAACCCCATGCGTCTGCGCACCAACCGACACTTTGAAGTCCTG
AAGAATCACACGGATGTGTGGTTCGTGTTCCTGCCTGCCGCCCCCGTGGAGACCCAGGCC
CCTGCCATCCTGGCCCTGGCCGCCCTGCTCATCCTTCTGGGCCTCCTGTCCACAGCCCTC
CTGGGGCACCTGCTCTGCTTCCACATTTATCTCATGTGGCACAAGCTCACCACCTATGAG
TACATCGTGCAGCACCGCCCACCACAGGAGGCCAAGGGGGTTCACAGGGAGCTCGAGTCA
TGTCCTCCCAAGATGCGGCCCATTCAGGAGATGGAGTTCTACATGCGGACCTTCAGACAT
ATGCGCCCAGAGCCCCCTGGCCAGGCCGGGCCAGCAGCAGTGAATGCCAAACACTCTCGC
CCTGCCTCCCCGGATCCGACCCCAGGTAGGAGGGACTGTGCTGGGCCTCCGGTCCAGGTG
GAGTGGGATAGAAAGAAGCCTCTACCCTGGCGCTCGCCTCTGCTTCTTTTGGCGATGTGG
GGCCCTCAGGCTCCCCCGTGTCTCTGCAGAAAAAGAGGAAGAGGCGCGTGTATAAAGTGC
GAACGTCTGAGACCTCGGATCCGGCGTCGGGGCCTAGGGCCCCCAGCCGCCGCTCCAGCT
CGTCGACGGATTCCGCGGACGCCAGCCCTGTGCACGCCGCTGGCCCTGCCGGCGCCTACC
ACTCGGCGTCGGCAGAGTCCGTGGACGAGATTCCAGTGGCGCAGACGCGCCTGGGCAGCG
CCGCTCTGGCCGCCCCGCGGGGCCGGGGCCGACAGCCCACGCTGGCGCGGCAGGCGCGTG
CGCCCGCCGTTTTCG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZDHHC1
Symbol Nomenclature : ZDHHC1
SYNONYM : C16orf1; HSU90653; ZNF377
Designation : DHHC domain-containing cysteine-rich protein 1|DHHC-1|DHHC-domain-containing cysteine-rich protein|probable palmitoyltransferase ZDHHC1|zinc finger DHHC domain-containing protein 1|zinc finger protein 377|zinc finger, DHHC domain containing 1
Full Nomenclature : zinc finger, DHHC-type containing 1
GENEID : 29800
HGNC : 17916
Vega : OTTHUMG00000137522
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00515835        ATGTACAAGATGAACATCTGCAACAAGCCCTCCAACAAGACGGCCCCTGAGAAGAGTGTG
NM_013304.2         ATGTACAAGATGAACATCTGCAACAAGCCCTCCAACAAGACGGCCCCTGAGAAGAGTGTG
XM_005255914.1      ATGTACAAGATGAACATCTGCAACAAGCCCTCCAACAAGACGGCCCCTGAGAAGAGTGTG
                    ************************************************************

HsCD00515835        TGGACGGCACCGGCACAGCCCAGCGGACCCTCCCCTGAGCTGCAGGGCCAGCGATCCCGC
NM_013304.2         TGGACGGCACCGGCACAGCCCAGCGGACCCTCCCCTGAGCTGCAGGGCCAGCGATCCCGC
XM_005255914.1      TGGACGGCACCGGCACAGCCCAGCGGACCCTCCCCTGAGCTGCAGGGCCAGCGATCCCGC
                    ************************************************************

HsCD00515835        CGGAATGGGTGGAGCTGGCCCCCTCACCCGCTCCAGATTGTGGCCTGGCTGCTGTACCTC
NM_013304.2         CGGAATGGGTGGAGCTGGCCCCCTCACCCGCTCCAGATTGTGGCCTGGCTGCTGTACCTC
XM_005255914.1      CGGAATGGGTGGAGCTGGCCCCCTCACCCGCTCCAGATTGTGGCCTGGCTGCTGTACCTC
                    ************************************************************

HsCD00515835        TTCTTTGCTGTGATCGGCTTTGGGATCCTTGTTCCCCTCCTGCCTCACCACTGGGTGCCC
NM_013304.2         TTCTTTGCTGTGATCGGCTTTGGGATCCTTGTTCCCCTCCTGCCTCACCACTGGGTGCCC
XM_005255914.1      TTCTTTGCTGTGATCGGCTTTGGGATCCTTGTTCCCCTCCTGCCTCACCACTGGGTGCCC
                    ************************************************************

HsCD00515835        GCTGGCTACGCTTGCATGGGCGCCATCTTTGCTGGCCACCTTGTGGTGCACCTGACCGCC
NM_013304.2         GCTGGCTACGCTTGCATGGGCGCCATCTTTGCTGGCCACCTTGTGGTGCACCTGACCGCC
XM_005255914.1      GCTGGCTACGCTTGCATGGGCGCCATCTTTGCTGGCCACCTTGTGGTGCACCTGACCGCC
                    ************************************************************

HsCD00515835        GTCTCCATCGATCCAGCAGATGCCAACGTGCGGGACAAGAGCTATGCGGGGCCCCTGCCC
NM_013304.2         GTCTCCATCGATCCAGCAGATGCCAACGTGCGGGACAAGAGCTATGCGGGGCCCCTGCCC
XM_005255914.1      GTCTCCATCGATCCAGCAGATGCCAACGTGCGGGACAAGAGCTATGCGGGGCCCCTGCCC
                    ************************************************************

HsCD00515835        ATCTTCAACCGAAGCCAGCACGCACATGTCATTGAAGACCTGCACTGCAACTTGTGCAAC
NM_013304.2         ATCTTCAACCGAAGCCAGCACGCACATGTCATTGAAGACCTGCACTGCAACTTGTGCAAC
XM_005255914.1      ATCTTCAACCGAAGCCAGCACGCACATGTCATTGAAGACCTGCACTGCAACTTGTGCAAC
                    ************************************************************

HsCD00515835        GTGGATGTGAGCGCTCGCTCCAAGCACTGCAGCGCCTGCAACAAGTGCGTGTGCGGTTTC
NM_013304.2         GTGGATGTGAGCGCTCGCTCCAAGCACTGCAGCGCCTGCAACAAGTGCGTGTGCGGTTTC
XM_005255914.1      GTGGATGTGAGCGCTCGCTCCAAGCACTGCAGCGCCTGCAACAAGTGCGTGTGCGGTTTC
                    ************************************************************

HsCD00515835        GACCACCACTGCAAGTGGCTCAACAACTGTGTGGGCGAGCGGAACTACCGGCTCTTTCTA
NM_013304.2         GACCACCACTGCAAGTGGCTCAACAACTGTGTGGGCGAGCGGAACTACCGGCTCTTTCTA
XM_005255914.1      GACCACCACTGCAAGTGGCTCAACAACTGTGTGGGCGAGCGGAACTACCGGCTCTTTCTA
                    ************************************************************

HsCD00515835        CACAGTGTTGCATCCGCTTTACTGGGCGTCCTGCTCCTGGTGCTGGTGGCCACATATGTC
NM_013304.2         CACAGTGTTGCATCCGCTTTACTGGGCGTCCTGCTCCTGGTGCTGGTGGCCACATATGTC
XM_005255914.1      CACAGTGTTGCATCCGCTTTACTGGGCGTCCTGCTCCTGGTGCTGGTGGCCACATATGTC
                    ************************************************************

HsCD00515835        TTCGTGGAGTTCTTTGTCAACCCCATGCGTCTGCGCACCAACCGACACTTTGAAGTCCTG
NM_013304.2         TTCGTGGAGTTCTTTGTCAACCCCATGCGTCTGCGCACCAACCGACACTTTGAAGTCCTG
XM_005255914.1      TTCGTGGAGTTCTTTGTCAACCCCATGCGTCTGCGCACCAACCGACACTTTGAAGTCCTG
                    ************************************************************

HsCD00515835        AAGAATCACACGGATGTGTGGTTCGTGTTCCTGCCTGCCGCCCCCGTGGAGACCCAGGCC
NM_013304.2         AAGAATCACACGGATGTGTGGTTCGTGTTCCTGCCTGCCGCCCCCGTGGAGACCCAGGCC
XM_005255914.1      AAGAATCACACGGATGTGTGGTTCGTGTTCCTGCCTGCCGCCCCCGTGGAGACCCAGGCC
                    ************************************************************

HsCD00515835        CCTGCCATCCTGGCCCTGGCCGCCCTGCTCATCCTTCTGGGCCTCCTGTCCACAGCCCTC
NM_013304.2         CCTGCCATCCTGGCCCTGGCCGCCCTGCTCATCCTTCTGGGCCTCCTGTCCACAGCCCTC
XM_005255914.1      CCTGCCATCCTGGCCCTGGCCGCCCTGCTCATCCTTCTGGGCCTCCTGTCCACAGCCCTC
                    ************************************************************

HsCD00515835        CTGGGGCACCTGCTCTGCTTCCACATTTATCTCATGTGGCACAAGCTCACCACCTATGAG
NM_013304.2         CTGGGGCACCTGCTCTGCTTCCACATTTATCTCATGTGGCACAAGCTCACCACCTATGAG
XM_005255914.1      CTGGGGCACCTGCTCTGCTTCCACATTTATCTCATGTGGCACAAGCTCACCACCTATGAG
                    ************************************************************

HsCD00515835        TACATCGTGCAGCACCGCCCACCACAGGAGGCCAAGGGGGTTCACAGGGAGCTCGAGTCA
NM_013304.2         TACATCGTGCAGCACCGCCCACCACAGGAGGCCAAGGGGGTTCACAGGGAGCTCGAGTCA
XM_005255914.1      TACATCGTGCAGCACCGCCCACCACAGGAGGCCAAGGGGGTTCACAGGGAGCTCGAGTCA
                    ************************************************************

HsCD00515835        TGTCCTCCCAAGATGCGGCCCATTCAGGAGATGGAGTTCTACATGCGGACCTTCAGACAT
NM_013304.2         TGTCCTCCCAAGATGCGGCCCATTCAGGAGATGGAGTTCTACATGCGGACCTTCAGACAT
XM_005255914.1      TGTCCTCCCAAGATGCGGCCCATTCAGGAGATGGAGTTCTACATGCGGACCTTCAGACAT
                    ************************************************************

HsCD00515835        ATGCGCCCAGAGCCCCCTGGCCAGGCCGGGCCAGCAGCAGTGAATGCCAAACACTCTCGC
NM_013304.2         ATGCGCCCAGAGCCCCCTGGCCAGGCCGGGCCAGCAGCAGTGAATGCCAAACACTCTCGC
XM_005255914.1      ATGCGCCCAGAGCCCCCTGGCCAGGCCGGGCCAGCAGCAGTGAATGCCAATCCCTCCCAG
                    **************************************************:*.*** *. 

HsCD00515835        CCTGCCTCCCCGGATCCGACCCCAGGTAGGAGGGACTGTGCTGGGCCTCCGGTCCAGGTG
NM_013304.2         CCTGCCTCCCCGGATCCGACCCCAGGTAGGAGGGACTGTGCTGGGCCTCCGGTCCAGGTG
XM_005255914.1      TTTCTTGCCACCCGTGGCCAAGCGG-----------------------------------
                      *    **.*  .*   ... *.*                                   

HsCD00515835        GAGTGGGATAGAAAGAAGCCTCTACCCTGGCGCTCGCCTCTGCTTCTTTTGGCGATGTGG
NM_013304.2         GAGTGGGATAGAAAGAAGCCTCTACCCTGGCGCTCGCCTCTGCTTCTTTTGGCGATGTGG
XM_005255914.1      ------------------------------AACCTCCACCACCCTCTTCCCCAGACACTC
                                                  ..*   *. *: * ****    .** .   

HsCD00515835        GGCCCTCAGGCTCCCCCGTGTCTCTGCAGAAAAAGAGGAAGAGGCGCGTGTATAAAGTGC
NM_013304.2         GGCCCTCAGGCTCCCCCGTGTCTCTGCAGAAAAAGAGGAAGAGGCGCGTGTATAAAGTGC
XM_005255914.1      TCGCCCTGCCTCCCCGGATCCGACCCCAGAAAAAGAGGAAGAGGCGCGTGTATAAAGTGC
                       **  .    ***  .*   :*  **********************************

HsCD00515835        GAACGTCTGAGACCTCGGATCCGGCGTCGGGGCCTAGGGCCCCCAGCCGCCGCTCCAGCT
NM_013304.2         GAACGTCTGAGACCTCGGATCCGGCGTCGGGGCCTAGGGCCCCCAGCCGCCGCTCCAGCT
XM_005255914.1      GAACGTCTGAGACCTCGGATCCGGCGTCGGGGCCTAGGGCCCCCAGCCGCCGCTCCAGCT
                    ************************************************************

HsCD00515835        CGTCGACGGATTCCGCGGACGCCAGCCCTGTGCACGCCGCTGGCCCTGCCGGCGCCTACC
NM_013304.2         CGTCGACGGATTCCGCGGACGCCAGCCCTGTGCACGCCGCTGGCCCTGCCGGCGCCTACC
XM_005255914.1      CGTCGACGGATTCCGCGGACGCCAGCCCTGTGCACGCCGCTGGCCCTGCCGGCGCCTACC
                    ************************************************************

HsCD00515835        ACTCGGCGTCGGCAGAGTCCGTGGACGAGATTCCAGTGGCGCAGACGCGCCTGGGCAGCG
NM_013304.2         ACTCGGCGTCGGCAGAGTCCGTGGACGAGATTCCAGTGGCGCAGACGCGCCTGGGCAGCG
XM_005255914.1      ACTCGGCGTCGGCAGAGTCCGTGGACGAGATTCCAGTGGCGCAGACGCGCCTGGGCAGCG
                    ************************************************************

HsCD00515835        CCGCTCTGGCCGCCCCGCGGGGCCGGGGCCGACAGCCCACGCTGGCGCGGCAGGCGCGTG
NM_013304.2         CCGCTCTGGCCGCCCCGCGGGGCCGGGGCCGACAGCCCACGCTGGCGCGGCAGGCGCGTG
XM_005255914.1      CCGCTCTGGCCGCCCCGCGGGGCCGGGGCCGACAGCCCACGCTGGCGCGGCAGGCGCGTG
                    ************************************************************

HsCD00515835        CGCCCGCCGTTTTCG---------------------------------------------
NM_013304.2         CGCCCGCCGTTTTCGTGA------------------------------------------
XM_005255914.1      CGCCCGCCGTTTTCGTGAGCCCGAGCAGCGGCGAGCCCAGGGCGCCGGGCGGCCGGGAGG
                    ***************                                             

HsCD00515835        --------------
NM_013304.2         --------------
XM_005255914.1      CTGGTCTGGCTTAG
                                  

Protein

HPRD : 15707

Clone

Cloning Information : 670013
Original Clone ID : ccsbBroadEn_03094

Organism


TAX_ID : 9606
Species Specific ID: 29800

Genome

Chromosome : 16
Map Location : 16q22.1
Ensembl : ENSG00000159714

Reagents

Labome : ZDHHC1-antibody