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CHRND (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CHRND
Gene Name:  cholinergic receptor, nicotinic, delta (muscle)
Sequence             
Original Clone ID: ccsbBroadEn_00311
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC093923.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00515889

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1552nts         Open reading frame : 1 to 1552
Reference Sequence Alignment

ATGGAGGGGCCAGTGCTGACACTGGGGCTGCTGGCTGCCCTGGCGGTGTGTGGCAGCTGG
GGGCTGAACGAGGAGGAGCGGCTGATCCGGCACCTGTTTCAAGAGAAGGGCTACAACAAG
GAGCTCCGGCCCGTGGCACACAAAGAGGAGAGTGTGGACGTTGCCCTGGCCCTCACACTC
TCCAACCTCATCTCCCTGAAAGAAGTTGAGGAGACCCTCACTACCAATGTGTGGATAGAG
CACGGCTGGACAGACAACCGGCTGAAGTGGAATGCTGAAGAATTTGGAAACATCAGTGTC
CTGCGCCTCCCCCCGGACATGGTGTGGCTCCCAGAGATTGTGCTGGAGAACAACAATGAC
GGCTCCTTCCAGATCTCCTACTCCTGCAACGTGCTTGTCTACCACTACGGCTTCGTGTAC
TGGCTGCCACCTGCCATCTTCCGCTCCTCCTGCCCCATCTCTGTCACCTATTTCCCCTTC
GACTGGCAGAACTGCTCCCTCAAGTTCAGTTCCCTCAAGTATACGGCCAAAGAGATCACC
CTGAGCCTGAAACAGGATGCCAAGGAGAACCGCACCTACCCCGTGGAGTGGATCATCATT
GATCCTGAAGGCTTCACAGAGAACGGGGAGTGGGAGATAGTCCACCGGCCGGCCAGGGTC
AACGTGGACCCCAGAGCCCCTCTGGACAGCCCCAGCCGCCAGGACATCACCTTCTACCTC
ATCATCCGCCGCAAGCCCCTCTTCTACATCATCAACATCCTGGTGCCCTGCGTGCTCATC
TCCTTCATGGTCAACCTGGTCTTCTACCTACCGGCTGACAGTGGTGAGAAGACATCAGTG
GCCATCTCGGTGCTCCTGGCTCAGTCTGTCTTCCTGCTGCTCATCTCCAAGCGTCTGCCT
GCCACATCCATGGCCATCCCCCTTATCGGCAAGTTCCTGCTCTTCGGCATGGTGCTGGTC
ACCATGGTTGTGGTGATCTGTGTCATCGTGCTCAACATCCACTTCCGAACACCCAGCACC
CATGTGCTGTCTGAGGGGGTCAAGAAGCTCTTCCTGGAGACCCTGCCGGAGCTCCTGCAC
ATGTCCCGCCCAGCAGAGGATGGACCCAGCCCTGGGGCCCTGGTGCGGAGGAGCAGCTCC
CTGGGATACATCTCCAAGGCCGAGGAGTACTTCCTGCTCAAGTCCCGCAGTGACCTCATG
TTCGAGAAGCAGTCAGAGCGGCATGGGCTGGCCAGGCGCCTCACCACTGCACGCCGGCCC
CCAGCAAGCTCTGAGCAGGCCCAGCAGGAACTCTTCAATGAGCTGAAGCCAGCTGTGGAT
GGGGCAAACTTCATTGTTAACCACATGAGGGACCAGAACAATTACAATGAGGAGAAAGAC
AGCTGGAACCGAGTGGCCCGCACAGTGGACCGCCTCTGCCTGTTTGTGGTGACGCCTGTC
ATGGTGGTGGGCACAGCCTGGATCTTCCTGCAGGGCGTTTACAACCAGCCACCACCCCAG
CCTTTTCCTGGGGACCCCTACTCCTACAACGTGCAGGACAAGCGCTTCATC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CHRND
Symbol Nomenclature : CHRND
SYNONYM : ACHRD; CMS2A; FCCMS; SCCMS
Designation : acetylcholine receptor subunit delta|acetylcholine receptor, nicotinic, delta (muscle)|cholinergic receptor, nicotinic, delta polypeptide
Full Nomenclature : cholinergic receptor, nicotinic, delta (muscle)
GENEID : 1144
HGNC : 1965
MIM : 100720
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00515889        ATGGAGGGGCCAGTGCTGACACTGGGGCTGCTGGCTGCCCTGGCGGTGTGTGGCAGCTGG
NM_001256657.1      ATGGAGGGGCCAGTGCTGACACTGGGGCTGCTGGCTGCCCTGGCGGTGTGTGGCAGCTGG
NM_000751.2         ATGGAGGGGCCAGTGCTGACACTGGGGCTGCTGGCTGCCCTGGCGGTGTGTGGCAGCTGG
                    ************************************************************

HsCD00515889        GGGCTGAACGAGGAGGAGCGGCTGATCCGGCACCTGTTTCAAGAGAAGGGCTACAACAAG
NM_001256657.1      GGGCTGAACGAGGAGGAGCGGCTGATCCGGCACCTGTTTCAAGAGAAGGGCTACAACAAG
NM_000751.2         GGGCTGAACGAGGAGGAGCGGCTGATCCGGCACCTGTTTCAAGAGAAGGGCTACAACAAG
                    ************************************************************

HsCD00515889        GAGCTCCGGCCCGTGGCACACAAAGAGGAGAGTGTGGACGTTGCCCTGGCCCTCACACTC
NM_001256657.1      GAGCTCCGGCCCGTGGCACACAAAGAGGAGAGTGTGGACGTTGCCCTGGCCCTCACACTC
NM_000751.2         GAGCTCCGGCCCGTGGCACACAAAGAGGAGAGTGTGGACGTTGCCCTGGCCCTCACACTC
                    ************************************************************

HsCD00515889        TCCAACCTCATCTCCCTGAAAGAAGTTGAGGAGACCCTCACTACCAATGTGTGGATAGAG
NM_001256657.1      TCCAACCTCATCTCCCT-------------------------------------------
NM_000751.2         TCCAACCTCATCTCCCTGAAAGAAGTTGAGGAGACCCTCACTACCAATGTGTGGATAGAG
                    *****************                                           

HsCD00515889        CACGGCTGGACAGACAACCGGCTGAAGTGGAATGCTGAAGAATTTGGAAACATCAGTGTC
NM_001256657.1      --GGGCTGGACAGACAACCGGCTGAAGTGGAATGCTGAAGAATTTGGAAACATCAGTGTC
NM_000751.2         CACGGCTGGACAGACAACCGGCTGAAGTGGAATGCTGAAGAATTTGGAAACATCAGTGTC
                       *********************************************************

HsCD00515889        CTGCGCCTCCCCCCGGACATGGTGTGGCTCCCAGAGATTGTGCTGGAGAACAACAATGAC
NM_001256657.1      CTGCGCCTCCCCCCGGACATGGTGTGGCTCCCAGAGATTGTGCTGGAGAACAACAATGAC
NM_000751.2         CTGCGCCTCCCCCCGGACATGGTGTGGCTCCCAGAGATTGTGCTGGAGAACAACAATGAC
                    ************************************************************

HsCD00515889        GGCTCCTTCCAGATCTCCTACTCCTGCAACGTGCTTGTCTACCACTACGGCTTCGTGTAC
NM_001256657.1      GGCTCCTTCCAGATCTCCTACTCCTGCAACGTGCTTGTCTACCACTACGGCTTCGTGTAC
NM_000751.2         GGCTCCTTCCAGATCTCCTACTCCTGCAACGTGCTTGTCTACCACTACGGCTTCGTGTAC
                    ************************************************************

HsCD00515889        TGGCTGCCACCTGCCATCTTCCGCTCCTCCTGCCCCATCTCTGTCACCTATTTCCCCTTC
NM_001256657.1      TGGCTGCCACCTGCCATCTTCCGCTCCTCCTGCCCCATCTCTGTCACCTATTTCCCCTTC
NM_000751.2         TGGCTGCCACCTGCCATCTTCCGCTCCTCCTGCCCCATCTCTGTCACCTATTTCCCCTTC
                    ************************************************************

HsCD00515889        GACTGGCAGAACTGCTCCCTCAAGTTCAGTTCCCTCAAGTATACGGCCAAAGAGATCACC
NM_001256657.1      GACTGGCAGAACTGCTCCCTCAAGTTCAGTTCCCTCAAGTATACGGCCAAAGAGATCACC
NM_000751.2         GACTGGCAGAACTGCTCCCTCAAGTTCAGTTCCCTCAAGTATACGGCCAAAGAGATCACC
                    ************************************************************

HsCD00515889        CTGAGCCTGAAACAGGATGCCAAGGAGAACCGCACCTACCCCGTGGAGTGGATCATCATT
NM_001256657.1      CTGAGCCTGAAACAGGATGCCAAGGAGAACCGCACCTACCCCGTGGAGTGGATCATCATT
NM_000751.2         CTGAGCCTGAAACAGGATGCCAAGGAGAACCGCACCTACCCCGTGGAGTGGATCATCATT
                    ************************************************************

HsCD00515889        GATCCTGAAGGCTTCACAGAGAACGGGGAGTGGGAGATAGTCCACCGGCCGGCCAGGGTC
NM_001256657.1      GATCCTGAAGGCTTCACAGAGAACGGGGAGTGGGAGATAGTCCACCGGCCGGCCAGGGTC
NM_000751.2         GATCCTGAAGGCTTCACAGAGAACGGGGAGTGGGAGATAGTCCACCGGCCGGCCAGGGTC
                    ************************************************************

HsCD00515889        AACGTGGACCCCAGAGCCCCTCTGGACAGCCCCAGCCGCCAGGACATCACCTTCTACCTC
NM_001256657.1      AACGTGGACCCCAGAGCCCCTCTGGACAGCCCCAGCCGCCAGGACATCACCTTCTACCTC
NM_000751.2         AACGTGGACCCCAGAGCCCCTCTGGACAGCCCCAGCCGCCAGGACATCACCTTCTACCTC
                    ************************************************************

HsCD00515889        ATCATCCGCCGCAAGCCCCTCTTCTACATCATCAACATCCTGGTGCCCTGCGTGCTCATC
NM_001256657.1      ATCATCCGCCGCAAGCCCCTCTTCTACATCATCAACATCCTGGTGCCCTGCGTGCTCATC
NM_000751.2         ATCATCCGCCGCAAGCCCCTCTTCTACATCATCAACATCCTGGTGCCCTGCGTGCTCATC
                    ************************************************************

HsCD00515889        TCCTTCATGGTCAACCTGGTCTTCTACCTACCGGCTGACAGTGGTGAGAAGACATCAGTG
NM_001256657.1      TCCTTCATGGTCAACCTGGTCTTCTACCTACCGGCTGACAGTGGTGAGAAGACATCAGTG
NM_000751.2         TCCTTCATGGTCAACCTGGTCTTCTACCTACCGGCTGACAGTGGTGAGAAGACATCAGTG
                    ************************************************************

HsCD00515889        GCCATCTCGGTGCTCCTGGCTCAGTCTGTCTTCCTGCTGCTCATCTCCAAGCGTCTGCCT
NM_001256657.1      GCCATCTCGGTGCTCCTGGCTCAGTCTGTCTTCCTGCTGCTCATCTCCAAGCGTCTGCCT
NM_000751.2         GCCATCTCGGTGCTCCTGGCTCAGTCTGTCTTCCTGCTGCTCATCTCCAAGCGTCTGCCT
                    ************************************************************

HsCD00515889        GCCACATCCATGGCCATCCCCCTTATCGGCAAGTTCCTGCTCTTCGGCATGGTGCTGGTC
NM_001256657.1      GCCACATCCATGGCCATCCCCCTTATCGGCAAGTTCCTGCTCTTCGGCATGGTGCTGGTC
NM_000751.2         GCCACATCCATGGCCATCCCCCTTATCGGCAAGTTCCTGCTCTTCGGCATGGTGCTGGTC
                    ************************************************************

HsCD00515889        ACCATGGTTGTGGTGATCTGTGTCATCGTGCTCAACATCCACTTCCGAACACCCAGCACC
NM_001256657.1      ACCATGGTTGTGGTGATCTGTGTCATCGTGCTCAACATCCACTTCCGAACACCCAGCACC
NM_000751.2         ACCATGGTTGTGGTGATCTGTGTCATCGTGCTCAACATCCACTTCCGAACACCCAGCACC
                    ************************************************************

HsCD00515889        CATGTGCTGTCTGAGGGGGTCAAGAAGCTCTTCCTGGAGACCCTGCCGGAGCTCCTGCAC
NM_001256657.1      CATGTGCTGTCTGAGGGGGTCAAGAAGCTCTTCCTGGAGACCCTGCCGGAGCTCCTGCAC
NM_000751.2         CATGTGCTGTCTGAGGGGGTCAAGAAGCTCTTCCTGGAGACCCTGCCGGAGCTCCTGCAC
                    ************************************************************

HsCD00515889        ATGTCCCGCCCAGCAGAGGATGGACCCAGCCCTGGGGCCCTGGTGCGGAGGAGCAGCTCC
NM_001256657.1      ATGTCCCGCCCAGCAGAGGATGGACCCAGCCCTGGGGCCCTGGTGCGGAGGAGCAGCTCC
NM_000751.2         ATGTCCCGCCCAGCAGAGGATGGACCCAGCCCTGGGGCCCTGGTGCGGAGGAGCAGCTCC
                    ************************************************************

HsCD00515889        CTGGGATACATCTCCAAGGCCGAGGAGTACTTCCTGCTCAAGTCCCGCAGTGACCTCATG
NM_001256657.1      CTGGGATACATCTCCAAGGCCGAGGAGTACTTCCTGCTCAAGTCCCGCAGTGACCTCATG
NM_000751.2         CTGGGATACATCTCCAAGGCCGAGGAGTACTTCCTGCTCAAGTCCCGCAGTGACCTCATG
                    ************************************************************

HsCD00515889        TTCGAGAAGCAGTCAGAGCGGCATGGGCTGGCCAGGCGCCTCACCACTGCACGCCGGCCC
NM_001256657.1      TTCGAGAAGCAGTCAGAGCGGCATGGGCTGGCCAGGCGCCTCACCACTGCACGCCGGCCC
NM_000751.2         TTCGAGAAGCAGTCAGAGCGGCATGGGCTGGCCAGGCGCCTCACCACTGCACGCCGGCCC
                    ************************************************************

HsCD00515889        CCAGCAAGCTCTGAGCAGGCCCAGCAGGAACTCTTCAATGAGCTGAAGCCAGCTGTGGAT
NM_001256657.1      CCAGCAAGCTCTGAGCAGGCCCAGCAGGAACTCTTCAATGAGCTGAAGCCAGCTGTGGAT
NM_000751.2         CCAGCAAGCTCTGAGCAGGCCCAGCAGGAACTCTTCAATGAGCTGAAGCCAGCTGTGGAT
                    ************************************************************

HsCD00515889        GGGGCAAACTTCATTGTTAACCACATGAGGGACCAGAACAATTACAATGAGGAGAAAGAC
NM_001256657.1      GGGGCAAACTTCATTGTTAACCACATGAGGGACCAGAACAATTACAATGAGGAGAAAGAC
NM_000751.2         GGGGCAAACTTCATTGTTAACCACATGAGGGACCAGAACAATTACAATGAGGAGAAAGAC
                    ************************************************************

HsCD00515889        AGCTGGAACCGAGTGGCCCGCACAGTGGACCGCCTCTGCCTGTTTGTGGTGACGCCTGTC
NM_001256657.1      AGCTGGAACCGAGTGGCCCGCACAGTGGACCGCCTCTGCCTGTTTGTGGTGACGCCTGTC
NM_000751.2         AGCTGGAACCGAGTGGCCCGCACAGTGGACCGCCTCTGCCTGTTTGTGGTGACGCCTGTC
                    ************************************************************

HsCD00515889        ATGGTGGTGGGCACAGCCTGGATCTTCCTGCAGGGCGTTTACAACCAGCCACCACCCCAG
NM_001256657.1      ATGGTGGTGGGCACAGCCTGGATCTTCCTGCAGGGCGTTTACAACCAGCCACCACCCCAG
NM_000751.2         ATGGTGGTGGGCACAGCCTGGATCTTCCTGCAGGGCGTTTACAACCAGCCACCACCCCAG
                    ************************************************************

HsCD00515889        CCTTTTCCTGGGGACCCCTACTCCTACAACGTGCAGGACAAGCGCTTCATC---
NM_001256657.1      CCTTTTCCTGGGGACCCCTACTCCTACAACGTGCAGGACAAGCGCTTCATCTAG
NM_000751.2         CCTTTTCCTGGGGACCCCTACTCCTACAACGTGCAGGACAAGCGCTTCATCTAG
                    ***************************************************   

Pathways

Panther : Nicotinic acetylcholine receptor signaling pathway
Reactome : Acetylcholine Binding And Downstream Events
Reactome : Activation of Nicotinic Acetylcholine Receptors
Reactome : Highly sodium permeable acetylcholine nicotinic receptors
Reactome : Neuronal System
Reactome : Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome : Postsynaptic nicotinic acetylcholine receptors
Reactome : Presynaptic nicotinic acetylcholine receptors
Reactome : Transmission across Chemical Synapses

Protein

HPRD : 01516

Clone

Cloning Information : 669989
Original Clone ID : ccsbBroadEn_00311

Organism


TAX_ID : 9606
Species Specific ID: 1144

Genome

Chromosome : 2
Map Location : 2q37.1

Reagents

Labome : CHRND-antibody