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ARHGEF6 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ARHGEF6
Gene Name:  Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
Sequence             
Original Clone ID: ccsbBroadEn_11368
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC043505.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00515919

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1615nts         Open reading frame : 1 to 1615
Reference Sequence Alignment

ATGACGGAAAATGGAAGTCATCAGTTGATAGTAAAAGCAAGATTCAACTTTAAGCAGACT
AATGAGGATGAACTGTCAGTTTGTAAGGGGGACATCATTTACGTCACACGAGTTGAAGAA
GGAGGCTGGTGGGAAGGCACATTAAATGGGAGAACAGGCTGGTTCCCCAGTAATTATGTC
CGTGAAATTAAATCCAGTGAGAGACCTCTCTCCCCAAAAGCCGTCAAAGGATTTGAAACT
GCTCCACTTACCAAGAATTATTATACTGTGGTGTTACAGAACATCCTGGACACTGAAAAA
GAATATGCTAAAGAACTTCAGTCTCTTCTTGTTACTTACTTAAGACCCCTGCAGTCCAAT
AACAATCTGAGTACTGTGGAGGTTACATCTTTACTGGGAAACTTCGAGGAAGTATGCACA
TTTCAACAGACACTCTGCCAAGCCTTGGAAGAATGTTCAAAGTTTCCAGAAAACCAGCAC
AAAGTAGGAGGTTGTCTACTGAGTCTCATGCCTCATTTTAAATCTATGTATCTGGCTTAC
TGTGCAAACCATCCTTCAGCTGTAAATGTGCTCACTCAGCACAGTGATGAGTTGGAACAA
TTCATGGAAAATCAAGGTGCATCGAGCCCAGGTATCCTCATTTTAACAACAAACCTCAGC
AAACCATTCATGCGACTGGAGAAATATGTTACTCTCTTGCAAGAGTTAGAACGGCATATG
GAGGATACTCATCCAGATCATCAGGATATTCTGAAAGCAATCGTAGCATTCAAAACTCTC
ATGGGGCAATGTCAAGATCTGAGGAAGAGAAAACAGCTGGAGTTACAGATACTGTCCGAA
CCTATTCAGGCATGGGAAGGAGAAGATATTAAAAACTTGGGAAATGTGATTTTTATGTCA
CAAGTAATGGTGCAGTATGGAGCATGTGAGGAAAAAGAGGAGCGGTACCTTATGTTATTT
TCAAATGTCCTGATAATGTTATCTGCAAGTCCTCGGATGAGTGGCTTTATCTATCAGGGA
AAAATACCAATAGCAGGAACGGTGGTGACTAGATTAGATGAAATTGAAGGGAATGACTGC
ACATTTGAAATCACTGGTAACACAGTGGAGAGAATTGTGGTCCATTGTAACAACAACCAG
GACTTCCAGGAATGGTTGGAGCAGCTGAACAGACTGATCAGAGGACCTGCCTCTTGCAGT
TCATTATCCAAAACCTCATCGTCATCATGTAGTGCTCATTCTTCTTTTAGCTCTACCGGA
CAGCCCCGAGGACCCTTGGAGCCTCCTCAAATTATAAAACCGTGGAGTTTAAGTTGTCTA
CGACCTGCACCTCCACTTAGACCATCAGCAGCACTAGGTTATAAAGAGAGGATGTCTTAT
ATCTTAAAGGAGTCTAGTAAAAGCCCTAAAACGATGAAGAAATTTCTTCATAAAAGGAAG
ACTGAGAGAAAACCATCGGAGGAGGAATATGTGATTAGGAAAAGTACAGCTGCTCTGGAA
GAGGATGCTCAAATCCTTAAAGTGATCGAAGCCTACTGCACCAGCGCAAATTTTCAACAA
GGCCATGGCTCAAGTACTCGAAAAGATTCCATTCCACAAGTCCTACTCCCTGAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ARHGEF6
Symbol Nomenclature : ARHGEF6
SYNONYM : COOL2; Cool-2; MRX46; PIXA; alpha-PIX; alphaPIX
Designation : PAK-interacting exchange factor alpha|PAK-interacting exchange factor, alpha|Rac/Cdc42 guanine exchange factor (GEF) 6|rac/Cdc42 guanine nucleotide exchange factor 6|rho guanine nucleotide exchange factor 6
Full Nomenclature : Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
GENEID : 9459
Locus Tag : RP3-527F8.4
HGNC : 685
MIM : 300267
Vega : OTTHUMG00000022518
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00515919        ------------------------------------------------------------
NM_004840.2         ATGAATCCAGAAGAACAAATCGTGACATGGCTTATATCTTTGGGAGTTTTAGAGTCCCCT
XM_005262501.1      ------------------------------------------------------------
                                                                                

HsCD00515919        ------------------------------------------------------------
NM_004840.2         AAAAAGACCATCTGTGATCCGGAGGAGTTTTTAAAGTCCTCGCTGAAAAATGGGGTAGTT
XM_005262501.1      ------------------------------------------------------------
                                                                                

HsCD00515919        ------------------------------------------------------------
NM_004840.2         CTGTGCAAACTGATCAACAGACTCATGCCTGGCTCTGTGGAAAAGTTTTGTCTGGATCCC
XM_005262501.1      ------------------------------------------------------------
                                                                                

HsCD00515919        ------------------------------------------------------------
NM_004840.2         CAAACTGAAGCTGACTGCATCAACAACATCAATGACTTCCTGAAAGGATGTGCAACCCTC
XM_005262501.1      ------------------------------------------------------------
                                                                                

HsCD00515919        ------------------------------------------------------------
NM_004840.2         CAAGTGGAAATATTTGATCCTGATGACCTTTATTCAGGGGTCAATTTCTCCAAGGTACTG
XM_005262501.1      ------------------------------------------------------------
                                                                                

HsCD00515919        ------------------------------------------------------------
NM_004840.2         AGTACTCTTTTAGCTGTCAACAAAGCAACAGAAGATCAGCTATCAGAAAGACCATGTGGA
XM_005262501.1      ------------------------------------------------------------
                                                                                

HsCD00515919        ------------------------------------------------------------
NM_004840.2         CGTTCCTCTTCTCTTAGTGCTGCTAATACTTCTCAGACAAACCCACAGGGAGCAGTTTCT
XM_005262501.1      ------------------------------------------------------------
                                                                                

HsCD00515919        ------------------------------------------ATGACGGAAAATGGAAGT
NM_004840.2         AGCACAGTTTCAGGGCTGCAAAGGCAGTCAAAGACAGTGGAGATGACGGAAAATGGAAGT
XM_005262501.1      ------------------------------------------ATGACGGAAAATGGAAGT
                                                              ******************

HsCD00515919        CATCAGTTGATAGTAAAAGCAAGATTCAACTTTAAGCAGACTAATGAGGATGAACTGTCA
NM_004840.2         CATCAGTTGATAGTAAAAGCAAGATTCAACTTTAAGCAGACTAATGAGGATGAACTGTCA
XM_005262501.1      CATCAGTTGATAGTAAAAGCAAGATTCAACTTTAAGCAGACTAATGAGGATGAACTGTCA
                    ************************************************************

HsCD00515919        GTTTGTAAGGGGGACATCATTTACGTCACACGAGTTGAAGAAGGAGGCTGGTGGGAAGGC
NM_004840.2         GTTTGTAAGGGGGACATCATTTACGTCACACGAGTTGAAGAAGGAGGCTGGTGGGAAGGC
XM_005262501.1      GTTTGTAAGGGGGACATCATTTACGTCACACGAGTTGAAGAAGGAGGCTGGTGGGAAGGC
                    ************************************************************

HsCD00515919        ACATTAAATGGGAGAACAGGCTGGTTCCCCAGTAATTATGTCCGTGAAATTAAATCCAGT
NM_004840.2         ACATTAAATGGGAGAACAGGCTGGTTCCCCAGTAATTATGTCCGTGAAATTAAATCCAGT
XM_005262501.1      ACATTAAATGGGAGAACAGGCTGGTTCCCCAGTAATTATGTCCGTGAAATTAAATCCAGT
                    ************************************************************

HsCD00515919        GAGAGACCTCTCTCCCCAAAAGCCGTCAAAGGATTTGAAACTGCTCCACTTACCAAGAAT
NM_004840.2         GAGAGACCTCTCTCCCCAAAAGCCGTCAAAGGATTTGAAACTGCTCCACTTACCAAGAAT
XM_005262501.1      GAGAGACCTCTCTCCCCAAAAGCCGTCAAAGGATTTGAAACTGCTCCACTTACCAAGAAT
                    ************************************************************

HsCD00515919        TATTATACTGTGGTGTTACAGAACATCCTGGACACTGAAAAAGAATATGCTAAAGAACTT
NM_004840.2         TATTATACTGTGGTGTTACAGAACATCCTGGACACTGAAAAAGAATATGCTAAAGAACTT
XM_005262501.1      TATTATACTGTGGTGTTACAGAACATCCTGGACACTGAAAAAGAATATGCTAAAGAACTT
                    ************************************************************

HsCD00515919        CAGTCTCTTCTTGTTACTTACTTAAGACCCCTGCAGTCCAATAACAATCTGAGTACTGTG
NM_004840.2         CAGTCTCTTCTTGTTACTTACTTAAGACCCCTGCAGTCCAATAACAATCTGAGTACTGTG
XM_005262501.1      CAGTCTCTTCTTGTTACTTACTTAAGACCCCTGCAGTCCAATAACAATCTGAGTACTGTG
                    ************************************************************

HsCD00515919        GAGGTTACATCTTTACTGGGAAACTTCGAGGAAGTATGCACATTTCAACAGACACTCTGC
NM_004840.2         GAGGTTACATCTTTACTGGGAAACTTCGAGGAAGTATGCACATTTCAACAGACACTCTGC
XM_005262501.1      GAGGTTACATCTTTACTGGGAAACTTCGAGGAAGTATGCACATTTCAACAGACACTCTGC
                    ************************************************************

HsCD00515919        CAAGCCTTGGAAGAATGTTCAAAGTTTCCAGAAAACCAGCACAAAGTAGGAGGTTGTCTA
NM_004840.2         CAAGCCTTGGAAGAATGTTCAAAGTTTCCAGAAAACCAGCACAAAGTAGGAGGTTGTCTA
XM_005262501.1      CAAGCCTTGGAAGAATGTTCAAAGTTTCCAGAAAACCAGCACAAAGTAGGAGGTTGTCTA
                    ************************************************************

HsCD00515919        CTGAGTCTCATGCCTCATTTTAAATCTATGTATCTGGCTTACTGTGCAAACCATCCTTCA
NM_004840.2         CTGAGTCTCATGCCTCATTTTAAATCTATGTATCTGGCTTACTGTGCAAACCATCCTTCA
XM_005262501.1      CTGAGTCTCATGCCTCATTTTAAATCTATGTATCTGGCTTACTGTGCAAACCATCCTTCA
                    ************************************************************

HsCD00515919        GCTGTAAATGTGCTCACTCAGCACAGTGATGAGTTGGAACAATTCATGGAAAATCAAGGT
NM_004840.2         GCTGTAAATGTGCTCACTCAGCACAGTGATGAGTTGGAACAATTCATGGAAAATCAAGGT
XM_005262501.1      GCTGTAAATGTGCTCACTCAGCACAGTGATGAGTTGGAACAATTCATGGAAAATCAAGGT
                    ************************************************************

HsCD00515919        GCATCGAGCCCAGGTATCCTCATTTTAACAACAAACCTCAGCAAACCATTCATGCGACTG
NM_004840.2         GCATCGAGCCCAGGTATCCTCATTTTAACAACAAACCTCAGCAAACCATTCATGCGACTG
XM_005262501.1      GCATCGAGCCCAGGTATCCTCATTTTAACAACAAACCTCAGCAAACCATTCATGCGACTG
                    ************************************************************

HsCD00515919        GAGAAATATGTTACTCTCTTGCAAGAGTTAGAACGGCATATGGAGGATACTCATCCAGAT
NM_004840.2         GAGAAATATGTTACTCTCTTGCAAGAGTTAGAACGGCATATGGAGGATACTCATCCAGAT
XM_005262501.1      GAGAAATATGTTACTCTCTTGCAAGAGTTAGAACGGCATATGGAGGATACTCATCCAGAT
                    ************************************************************

HsCD00515919        CATCAGGATATTCTGAAAGCAATCGTAGCATTCAAAACTCTCATGGGGCAATGTCAAGAT
NM_004840.2         CATCAGGATATTCTGAAAGCAATCGTAGCATTCAAAACTCTCATGGGGCAATGTCAAGAT
XM_005262501.1      CATCAGGATATTCTGAAAGCAATCGTAGCATTCAAAACTCTCATGGGGCAATGTCAAGAT
                    ************************************************************

HsCD00515919        CTGAGGAAGAGAAAACAGCTGGAGTTACAGATACTGTCCGAACCTATTCAGGCATGGGAA
NM_004840.2         CTGAGGAAGAGAAAACAGCTGGAGTTACAGATACTGTCCGAACCTATTCAGGCATGGGAA
XM_005262501.1      CTGAGGAAGAGAAAACAGCTGGAGTTACAGATACTGTCCGAACCTATTCAGGCATGGGAA
                    ************************************************************

HsCD00515919        GGAGAAGATATTAAAAACTTGGGAAATGTGATTTTTATGTCACAAGTAATGGTGCAGTAT
NM_004840.2         GGAGAAGATATTAAAAACTTGGGAAATGTGATTTTTATGTCACAAGTAATGGTGCAGTAT
XM_005262501.1      GGAGAAGATATTAAAAACTTGGGAAATGTGATTTTTATGTCACAAGTAATGGTGCAGTAT
                    ************************************************************

HsCD00515919        GGAGCATGTGAGGAAAAAGAGGAGCGGTACCTTATGTTATTTTCAAATGTCCTGATAATG
NM_004840.2         GGAGCATGTGAGGAAAAAGAGGAGCGGTACCTTATGTTATTTTCAAATGTCCTGATAATG
XM_005262501.1      GGAGCATGTGAGGAAAAAGAGGAGCGGTACCTTATGTTATTTTCAAATGTCCTGATAATG
                    ************************************************************

HsCD00515919        TTATCTGCAAGTCCTCGGATGAGTGGCTTTATCTATCAGGGAAAAATACCAATAGCAGGA
NM_004840.2         TTATCTGCAAGTCCTCGGATGAGTGGCTTTATCTATCAGGGAAAAATACCAATAGCAGGA
XM_005262501.1      TTATCTGCAAGTCCTCGGATGAGTGGCTTTATCTATCAGGGAAAAATACCAATAGCAGGA
                    ************************************************************

HsCD00515919        ACGGTGGTGACTAGATTAGATGAAATTGAAGGGAATGACTGCACATTTGAAATCACTGGT
NM_004840.2         ACGGTGGTGACTAGATTAGATGAAATTGAAGGGAATGACTGCACATTTGAAATCACTGGT
XM_005262501.1      ACGGTGGTGACTAGATTAGATGAAATTGAAGGGAATGACTGCACATTTGAAATCACTGGT
                    ************************************************************

HsCD00515919        AACACAGTGGAGAGAATTGTGGTCCATTGTAACAACAACCAGGACTTCCAGGAATGGTTG
NM_004840.2         AACACAGTGGAGAGAATTGTGGTCCATTGTAACAACAACCAGGACTTCCAGGAATGGTTG
XM_005262501.1      AACACAGTGGAGAGAATTGTGGTCCATTGTAACAACAACCAGGACTTCCAGGAATGGTTG
                    ************************************************************

HsCD00515919        GAGCAGCTGAACAGACTGATCAGAGGACCTGCCTCTTGCAGTTCATTATCCAAAACCTCA
NM_004840.2         GAGCAGCTGAACAGACTGATCAGAGGACCTGCCTCTTGCAGTTCATTATCCAAAACCTCA
XM_005262501.1      GAGCAGCTGAACAGACTGATCAGAGGACCTGCCTCTTGCAGTTCATTATCCAAAACCTCA
                    ************************************************************

HsCD00515919        TCGTCATCATGTAGTGCTCATTCTTCTTTTAGCTCTACCGGACAGCCCCGAGGACCCTTG
NM_004840.2         TCGTCATCATGTAGTGCTCATTCTTCTTTTAGCTCTACCGGACAGCCCCGAGGACCCTTG
XM_005262501.1      TCGTCATCATGTAGTGCTCATTCTTCTTTTAGCTCTACCGGACAGCCCCGAGGACCCTTG
                    ************************************************************

HsCD00515919        GAGCCTCCTCAAATTATAAAACCGTGGAGTTTAAGTTGTCTACGACCTGCACCTCCACTT
NM_004840.2         GAGCCTCCTCAAATTATAAAACCGTGGAGTTTAAGTTGTCTACGACCTGCACCTCCACTT
XM_005262501.1      GAGCCTCCTCAAATTATAAAACCGTGGAGTTTAAGTTGTCTACGACCTGCACCTCCACTT
                    ************************************************************

HsCD00515919        AGACCATCAGCAGCACTAGGTTATAAAGAGAGGATGTCTTATATCTTAAAGGAGTCTAGT
NM_004840.2         AGACCATCAGCAGCACTAGGTTATAAAGAGAGGATGTCTTATATCTTAAAGGAGTCTAGT
XM_005262501.1      AGACCATCAGCAGCACTAGGTTATAAAGAGAGGATGTCTTATATCTTAAAGGAGTCTAGT
                    ************************************************************

HsCD00515919        AAAAGCCCTAAAACGATGAAGAAATTTCTTCATAAAAGGAAGACTGAGAGAAAACCATCG
NM_004840.2         AAAAGCCCTAAAACGATGAAGAAATTTCTTCATAAAAGGAAGACTGAGAGAAAACCATCG
XM_005262501.1      AAAAGCCCTAAAACGATGAAGAAATTTCTTCATAAAAGGAAGACTGAGAGAAAACCATCG
                    ************************************************************

HsCD00515919        GAGGAGGAATATGTGATTAGGAAAAGTACAGCTGCTCTGGAAGAGGATGCTCAAATCCTT
NM_004840.2         GAGGAGGAATATGTGATTAGGAAAAGTACAGCTGCTCTGGAAGAGGATGCTCAAATCCTT
XM_005262501.1      GAGGAGGAATATGTGATTAGGAAAAGTACAGCTGCTCTGGAAGAGGATGCTCAAATCCTT
                    ************************************************************

HsCD00515919        AAAGTGATCGAAGCCTACTGCACCAGCGCAAATTTTCAACAAGGCCATGGCTCAAGTACT
NM_004840.2         AAAGTGATCGAAGCCTACTGCACCAGCGCAAATTTTCAACAAGGCCATGGCTCAAGTACT
XM_005262501.1      AAAGTGATCGAAGCCTACTGCACCAGCGCAAATTTTCAACAAGGCCATGGCTCAAGTACT
                    ************************************************************

HsCD00515919        CGAAAAGATTCCATTCCACAAGTCCTACTCCCTGAG------------------------
NM_004840.2         CGAAAAGATTCCATTCCACAAGTCCTACTCCCTGAGGAAGAGAAACTCATCATTGAAGAA
XM_005262501.1      CGAAAAGATTCCATTCCACAAGTCCTACTCCCTGAGGAAGAGAAACTCATCATTGAAGAA
                    ************************************                        

HsCD00515919        ------------------------------------------------------------
NM_004840.2         ACCAGAAGCAACGGCCAGACCATCATGGAAGAAAAGAGCCTTGTTGATACTGTTTACGCC
XM_005262501.1      ACCAGAAGCAACGGCCAGACCATCATGGAAGAAAAGAGCCTTGTTGATACTGTTTACGCC
                                                                                

HsCD00515919        ------------------------------------------------------------
NM_004840.2         TTGAAGGACGAGGTCAGAGAACTGAAGCAGGAAAATAAAAGAATGAAGCAATGCCTGGAA
XM_005262501.1      TTGAAGGACGAGGTCAGAGAACTGAAGCAGGAAAATAAAAGAATGAAGCAATGCCTGGAA
                                                                                

HsCD00515919        ------------------------------------------------------------
NM_004840.2         GAAGAACTGAAATCAAGAAGGGACCTAGAAAAGCTGGTGCGGAGGCTTTTGAAGCAAACA
XM_005262501.1      GAAGAACTGAAATCAAGAAGGGACCTAGAAAAGCTGGTGCGGAGGCTTTTGAAGCAAACA
                                                                                

HsCD00515919        ---------------------------------------------------
NM_004840.2         GATGAGTGTATTCGAGGCGAGTCCAGTAGCAAGACCTCAATTCTTCCATAA
XM_005262501.1      GATGAGTGTATTCGAGGCGAGTCCAGTAGCAAGACCTCAATTCTTCCATAA
                                                                       

Pathways

NCI : CDC42 signaling events
NCI : Integrin-linked kinase signaling
NCI : Regulation of CDC42 activity
NCI : Regulation of RAC1 activity
Reactome : Cell death signalling via NRAGE, NRIF and NADE
Reactome : Cell junction organization
Reactome : Cell-Cell communication
Reactome : Cell-extracellular matrix interactions
Reactome : G alpha (12/13) signalling events
Reactome : GPCR downstream signaling
Reactome : NRAGE signals death through JNK
Reactome : Regulation of cytoskeletal remodeling and cell spreading by IPP complex components
Reactome : Rho GTPase cycle
Reactome : Signal Transduction
Reactome : Signaling by GPCR
Reactome : Signaling by Rho GTPases
Reactome : Signalling by NGF
Reactome : p75 NTR receptor-mediated signalling
WikiPathway : Regulation of Actin Cytoskeleton

Clone

Cloning Information : 669721
Original Clone ID : ccsbBroadEn_11368

Organism


TAX_ID : 9606
Species Specific ID: 9459

Genome

Chromosome : X
Map Location : Xq26.3
Ensembl : ENSG00000129675

Reagents

Labome : ARHGEF6-antibody