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KLHL31 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  KLHL31
Gene Name:  kelch-like family member 31
Sequence             
Original Clone ID: ccsbBroadEn_10129
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC137267.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00516186

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1903nts         Open reading frame : 1 to 1903
Reference Sequence Alignment

ATGGCACCCAAAAAGAAGATTGTCAAAAAGAACAAAGGAGATATCAATGAGATGACTATA
ATCGTAGAAGATAGCCCCCTAAACAAACTGAATGCTTTGAATGGGCTCCTAGAGGGAGGC
AATGGCCTTAGCTGCATTTCTTCTGAACTAACAGATGCTTCTTATGGCCCCAACCTCTTG
GAAGGTTTAAGTAAAATGCGGCAGGAGAACTTCTTATGTGACTTAGTCATTGGTACCAAA
ACCAAATCCTTTGATGTTCATAAGTCAGTCATGGCTTCATGCAGTGAGTATTTTTACAAC
ATCCTAAAAAAAGACCCGTCAATTCAGAGGGTGGATCTCAATGATATCTCACCACTAGGC
CTGGCCACTGTCATTGCATATGCCTACACTGGAAAGCTCACTCTCTCCTTGTATACAATA
GGAAGCATTATTTCTGCTGCTGTTTATCTTCAGATCCATACTCTTATAAAGATGTGCAGT
GATTTTCTGATACGGGAGATGAGTGTTGAGAATTGCATGTATGTTGTTAATATTGCTGAA
ACATACTCCCTAAAAAATGCAAAAGCAGCAGCCCAGAAATTTATTCGGGATAACTTCCTT
GAATTTGCAGAATCGGATCAGTTTATGAAACTTACATTTGAACAAATTAATGAACTTCTT
ATAGATGATGACTTACAGTTGCCTTCTGAGATAGTAGCATTCCAGATTGCAATGAAATGG
TTAGAATTTGACCAAAAGAGAGTAAAATACGCTGCAGATCTTTTGAGCAATATTCGCTTT
GGTACCATCTCTGCACAAGACCTGGTCAATTATGTTCAATCCGTACCAAGAATGATGCAA
GATGCTGATTGTCACAGACTTCTCGTAGATGCTATGAACTACCACTTGCTTCCATATCAT
CAAAACACATTGCAATCTAGGCGAACAAGAATCCGAGGTGGCTGCCGAGTCCTCGTCACT
GTTGGGGGACGCCCAGGCCTTACTGAGAAGTCCCTTAGCAGAGACATCCTGTATAGAGAC
CCTGAAAATGGATGGAGCAAGCTTACGGAAATGCCAGCCAAAAGTTTTAATCAGTGTGTG
GCTGTGATGGATGGATTTCTTTATGTAGCCGGTGGTGAAGACCAGAATGATGCAAGAAAT
CAAGCCAAGCATGCAGTCAGCAATTTCTGCAGATACGATCCCCGCTTCAACACCTGGATA
CACCTGGCCAGCATGAACCAGAAGCGCACGCACTTCAGCCTGAGCGTGTTCAACGGGCTC
GTGTACGCCGCGGGCGGCCGCAACGCAGAAGGAAGCCTGGCCTCGCTGGAGTGCTACGTG
CCCTCCACCAATCAGTGGCAGCCGAAGACGCCCCTGGAGGTGGCGCGCTGCTGCCACGCT
AGCGCGGTCGCCGACGGCCGCGTGCTGGTGACCGGAGGCTACATCGCCAACGCCTACTCG
CGCTCTGTGTGCGCCTACGACCCGGCCAGCGACTCGTGGCAGGAGCTGCCGAACCTCAGC
ACACCCCGGGGCTGGCACTGCGCGGTCACGCTGAGCGACAGAGTGTACGTGATGGGCGGC
AGCCAGCTGGGGCCGCGCGGGGAGCGCGTGGACGTGCTCACCGTGGAGTGCTACAGCCCC
GCGACCGGCCAGTGGAGCTACGCGGCGCCGCTGCAGGTGGGAGTGAGCACTGCGGGCGTC
TCGGCGCTGCATGGCCGCGCCTACCTGGTGGGGGGCTGGAACGAGGGCGAGAAGAAGTAC
AAGAAGTGCATCCAGTGCTTCAGCCCCGAGCTCAACGAGTGGACGGAGGACGACGAGCTA
CCCGAGGCCACTGTCGGCGTGTCCTGCTGCACCCTCTCGATGCCCAACAACGTGACTCGG
GAATCCCGGGCCAGTTCGGTATCTTCTGTGCCAGTCAGTATC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : KLHL31
Symbol Nomenclature : KLHL31
SYNONYM : BKLHD6; KBTBD1; KLHL; bA345L23.2
Designation : BTB and kelch domain-containing protein 6|kelch repeat and BTB (POZ) domain containing 1|kelch repeat and BTB domain-containing protein 1|kelch-like protein 31|kelch-like protein KLHL
Full Nomenclature : kelch-like family member 31
GENEID : 401265
HGNC : 21353
MIM : 610749
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00516186        ATGGCACCCAAAAAGAAGATTGTCAAAAAGAACAAAGGAGATATCAATGAGATGACTATA
NM_001003760.4      ATGGCACCCAAAAAGAAGATTGTCAAAAAGAACAAAGGAGATATCAATGAGATGACTATA
                    ************************************************************

HsCD00516186        ATCGTAGAAGATAGCCCCCTAAACAAACTGAATGCTTTGAATGGGCTCCTAGAGGGAGGC
NM_001003760.4      ATCGTAGAAGATAGCCCCCTAAACAAACTGAATGCTTTGAATGGGCTCCTAGAGGGAGGC
                    ************************************************************

HsCD00516186        AATGGCCTTAGCTGCATTTCTTCTGAACTAACAGATGCTTCTTATGGCCCCAACCTCTTG
NM_001003760.4      AATGGCCTTAGCTGCATTTCTTCTGAACTAACAGATGCTTCTTATGGCCCCAACCTCTTG
                    ************************************************************

HsCD00516186        GAAGGTTTAAGTAAAATGCGGCAGGAGAACTTCTTATGTGACTTAGTCATTGGTACCAAA
NM_001003760.4      GAAGGTTTAAGTAAAATGCGGCAGGAGAACTTCCTATGTGACTTAGTCATTGGTACCAAA
                    ********************************* **************************

HsCD00516186        ACCAAATCCTTTGATGTTCATAAGTCAGTCATGGCTTCATGCAGTGAGTATTTTTACAAC
NM_001003760.4      ACCAAATCCTTTGATGTTCATAAGTCAGTCATGGCTTCATGCAGTGAGTATTTTTACAAC
                    ************************************************************

HsCD00516186        ATCCTAAAAAAAGACCCGTCAATTCAGAGGGTGGATCTCAATGATATCTCACCACTAGGC
NM_001003760.4      ATCCTAAAAAAAGACCCGTCAATTCAGAGGGTGGATCTCAATGATATCTCACCACTAGGC
                    ************************************************************

HsCD00516186        CTGGCCACTGTCATTGCATATGCCTACACTGGAAAGCTCACTCTCTCCTTGTATACAATA
NM_001003760.4      CTGGCCACTGTCATTGCATATGCCTACACTGGAAAGCTCACTCTCTCCTTGTATACAATA
                    ************************************************************

HsCD00516186        GGAAGCATTATTTCTGCTGCTGTTTATCTTCAGATCCATACTCTTATAAAGATGTGCAGT
NM_001003760.4      GGAAGCATTATTTCTGCTGCTGTTTATCTTCAGATCCATACTCTTGTAAAGATGTGCAGT
                    *********************************************.**************

HsCD00516186        GATTTTCTGATACGGGAGATGAGTGTTGAGAATTGCATGTATGTTGTTAATATTGCTGAA
NM_001003760.4      GATTTTCTGATACGGGAGATGAGTGTTGAGAATTGCATGTATGTTGTTAATATTGCTGAA
                    ************************************************************

HsCD00516186        ACATACTCCCTAAAAAATGCAAAAGCAGCAGCCCAGAAATTTATTCGGGATAACTTCCTT
NM_001003760.4      ACATACTCCCTAAAAAATGCAAAAGCAGCAGCCCAGAAATTTATTCGGGATAACTTCCTT
                    ************************************************************

HsCD00516186        GAATTTGCAGAATCGGATCAGTTTATGAAACTTACATTTGAACAAATTAATGAACTTCTT
NM_001003760.4      GAATTTGCAGAATCGGATCAGTTTATGAAACTTACATTTGAACAAATTAATGAACTTCTT
                    ************************************************************

HsCD00516186        ATAGATGATGACTTACAGTTGCCTTCTGAGATAGTAGCATTCCAGATTGCAATGAAATGG
NM_001003760.4      ATAGATGATGACTTACAGTTGCCTTCTGAGATAGTAGCATTCCAGATTGCAATGAAATGG
                    ************************************************************

HsCD00516186        TTAGAATTTGACCAAAAGAGAGTAAAATACGCTGCAGATCTTTTGAGCAATATTCGCTTT
NM_001003760.4      TTAGAATTTGACCAAAAGAGAGTAAAATACGCTGCAGATCTTTTGAGCAATATTCGCTTT
                    ************************************************************

HsCD00516186        GGTACCATCTCTGCACAAGACCTGGTCAATTATGTTCAATCCGTACCAAGAATGATGCAA
NM_001003760.4      GGTACCATCTCTGCACAAGACCTGGTCAATTATGTTCAATCCGTACCAAGAATGATGCAA
                    ************************************************************

HsCD00516186        GATGCTGATTGTCACAGACTTCTCGTAGATGCTATGAACTACCACTTGCTTCCATATCAT
NM_001003760.4      GATGCTGATTGTCACAGACTTCTCGTAGATGCTATGAACTACCACTTGCTTCCATATCAT
                    ************************************************************

HsCD00516186        CAAAACACATTGCAATCTAGGCGAACAAGAATCCGAGGTGGCTGCCGAGTCCTCGTCACT
NM_001003760.4      CAAAACACATTGCAATCTAGGCGAACAAGAATCCGAGGTGGCTGCCGAGTCCTCGTCACT
                    ************************************************************

HsCD00516186        GTTGGGGGACGCCCAGGCCTTACTGAGAAGTCCCTTAGCAGAGACATCCTGTATAGAGAC
NM_001003760.4      GTTGGGGGACGCCCAGGCCTTACTGAGAAGTCCCTTAGCAGAGACATCTTGTATAGAGAC
                    ************************************************ ***********

HsCD00516186        CCTGAAAATGGATGGAGCAAGCTTACGGAAATGCCAGCCAAAAGTTTTAATCAGTGTGTG
NM_001003760.4      CCTGAAAATGGATGGAGCAAGCTTACGGAAATGCCAGCCAAAAGTTTTAATCAGTGTGTG
                    ************************************************************

HsCD00516186        GCTGTGATGGATGGATTTCTTTATGTAGCCGGTGGTGAAGACCAGAATGATGCAAGAAAT
NM_001003760.4      GCTGTGATGGATGGATTTCTTTATGTAGCCGGTGGTGAAGACCAGAATGATGCAAGAAAT
                    ************************************************************

HsCD00516186        CAAGCCAAGCATGCAGTCAGCAATTTCTGCAGATACGATCCCCGCTTCAACACCTGGATA
NM_001003760.4      CAAGCCAAGCATGCAGTCAGCAATTTCTGCAGATACGATCCCCGCTTCAACACCTGGATA
                    ************************************************************

HsCD00516186        CACCTGGCCAGCATGAACCAGAAGCGCACGCACTTCAGCCTGAGCGTGTTCAACGGGCTC
NM_001003760.4      CACCTGGCCAGCATGAACCAGAAGCGCACGCACTTCAGCCTGAGCGTGTTCAACGGGCTC
                    ************************************************************

HsCD00516186        GTGTACGCCGCGGGCGGCCGCAACGCAGAAGGAAGCCTGGCCTCGCTGGAGTGCTACGTG
NM_001003760.4      GTGTACGCCGCGGGCGGCCGCAACGCAGAAGGAAGCCTGGCCTCGCTGGAGTGCTACGTG
                    ************************************************************

HsCD00516186        CCCTCCACCAATCAGTGGCAGCCGAAGACGCCCCTGGAGGTGGCGCGCTGCTGCCACGCT
NM_001003760.4      CCCTCCACCAATCAGTGGCAGCCGAAGACGCCCCTGGAGGTGGCGCGCTGCTGCCACGCT
                    ************************************************************

HsCD00516186        AGCGCGGTCGCCGACGGCCGCGTGCTGGTGACCGGAGGCTACATCGCCAACGCCTACTCG
NM_001003760.4      AGCGCGGTCGCCGACGGCCGCGTGCTGGTGACCGGAGGCTACATAGCCAACGCCTACTCG
                    ********************************************.***************

HsCD00516186        CGCTCTGTGTGCGCCTACGACCCGGCCAGCGACTCGTGGCAGGAGCTGCCGAACCTCAGC
NM_001003760.4      CGCTCTGTGTGCGCCTACGACCCGGCCAGCGACTCGTGGCAGGAGCTGCCGAACCTCAGC
                    ************************************************************

HsCD00516186        ACACCCCGGGGCTGGCACTGCGCGGTCACGCTGAGCGACAGAGTGTACGTGATGGGCGGC
NM_001003760.4      ACACCCCGGGGCTGGCACTGCGCGGTCACGCTGAGCGACAGAGTGTACGTGATGGGCGGC
                    ************************************************************

HsCD00516186        AGCCAGCTGGGGCCGCGCGGGGAGCGCGTGGACGTGCTCACCGTGGAGTGCTACAGCCCC
NM_001003760.4      AGCCAGCTGGGGCCGCGCGGGGAGCGCGTGGACGTGCTCACCGTGGAGTGCTACAGCCCC
                    ************************************************************

HsCD00516186        GCGACCGGCCAGTGGAGCTACGCGGCGCCGCTGCAGGTGGGAGTGAGCACTGCGGGCGTC
NM_001003760.4      GCGACCGGCCAGTGGAGCTACGCGGCGCCGCTGCAGGTGGGAGTGAGCACTGCGGGCGTC
                    ************************************************************

HsCD00516186        TCGGCGCTGCATGGCCGCGCCTACCTGGTGGGGGGCTGGAACGAGGGCGAGAAGAAGTAC
NM_001003760.4      TCGGCGCTGCATGGCCGCGCCTACCTGGTGGGGGGCTGGAACGAGGGCGAGAAGAAGTAC
                    ************************************************************

HsCD00516186        AAGAAGTGCATCCAGTGCTTCAGCCCCGAGCTCAACGAGTGGACGGAGGACGACGAGCTA
NM_001003760.4      AAGAAGTGCATCCAGTGCTTCAGCCCCGAGCTCAACGAGTGGACGGAGGACGACGAGCTA
                    ************************************************************

HsCD00516186        CCCGAGGCCACTGTCGGCGTGTCCTGCTGCACCCTCTCGATGCCCAACAACGTGACTCGG
NM_001003760.4      CCCGAGGCCACTGTCGGCGTGTCCTGCTGCACCCTCTCGATGCCCAACAACGTGACTCGG
                    ************************************************************

HsCD00516186        GAATCCCGGGCCAGTTCGGTATCTTCTGTGCCAGTCAGTATC---
NM_001003760.4      GAATCCCGGGCCAGTTCGGTATCTTCTGTGCCAGTCAGTATCTGA
                    ******************************************   

Protein

SMART domain : BTB : Broad-Complex, Tramtrack and Bric a brac
SMART domain : Kelch :
SMART domain : BACK : BTB And C-terminal Kelch
HPRD : 13923

Clone

Cloning Information : 665771
Original Clone ID : ccsbBroadEn_10129

Organism


TAX_ID : 9606
Species Specific ID: 401265

Genome

Chromosome : 6
Map Location : 6p12.1
Ensembl : ENSG00000124743

Reagents

Labome : KLHL-antibody