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CAPN8 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CAPN8
Gene Name:  calpain 8
Sequence             
Original Clone ID: ccsbBroadEn_10096
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC157893.1
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00516210

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 2110nts         Open reading frame : 1 to 2110
Reference Sequence Alignment

ATGGCAGCCCAGGCAGCTGGTGTATCTAGGCAGCGGGCAGCCACTCAAGGTCTTGGCTCC
AACCAAAACGCTTTGAAGTACTTGGGCCAGGATTTCAAGACCCTGAGGCAACAGTGCTTG
GACTCAGGGGTCCTATTTAAGGACCCTGAGTTCCCAGCATGTCCATCAGCTTTGGGCTAC
AAGGATCTTGGACCAGGCTCTCCGCAAACTCAAGGCATCATCTGGAAGCGGCCCACGGAG
TTGTGTCCCAGCCCTCAGTTTATCGTTGGTGGAGCCACGCGCACAGACATTTGTCAGGGT
GGTCTAGGTGACTGCTGGCTTCTGGCTGCCATTGCCTCCCTGACCCTGAATGAAGAGCTG
CTTTACCGGGTGGTCCCCAGGGACCAGGACTTCCAGGAGAACTATGCGGGAATCTTTCAC
TTTCAGTTCTGGCAGTACGGAGAGTGGGTGGAGGTGGTCATTGACGACAGGCTGCCCACC
AAGAATGGACAGCTGCTCTTCCTACACTCGGAACAAGGCAATGAATTCTGGAGTGCCCTG
CTGGAGAAAGCCTATGCCAAGCTTAATGGTTGTTATGAGGCTCTCGCTGGAGGTTCCACA
GTGGAGGGGTTTGAGGATTTCACAGGTGGCATCTCTGAGTTTTATGACCTGAAGAAACCA
CCAGCCAATCTATATCAGATCATCCGGAAGGCCCTCTGTGCGGGGTCTCTGCTGGGCTGC
TCCATTGATGTCTACAGTGCAGCCGAAGCCGAAGCCATCACCAGCCAGAAGCTGGTTAAG
AGTCATGCGTACTCTGTCACTGGAGTCGAAGAGGTGAATTTCCAGGGCCATCCAGAGAAG
CTGATCAGACTCAGGAATCCATGGGGTGAAGTGGAGTGGTCGGGAGCCTGGAGCGATGAT
GCACCAGAGTGGAATCACATAGACCCCCGGCGGAAGGAAGAACTGGACAAGAAAGTTGAG
GATGGAGAATTCTGGATGTCACTTTCAGATTTCGTGAGGCAGTTCTCTCGGTTGGAGATC
TGCAACCTGTCCCCGGACTCTCTGAGTAGCGAGGAGGTGCACAAATGGAACCTGGTCCTG
TTCAACGGCCACTGGACCCGGGGCTCCACAGCTGGGGGCTGCCAGAACTACCCAGCCACG
TACTGGACCAATCCCCAGTTCAAAATCCGTTTGGATGAAGTGGATGAGGACCAGGAGGAG
AGCATCGGTGAACCCTGCTGTACAGTGCTGCTGGGCCTGATGCAGAAAAATCGCAGGTGG
CGGAAGCGGATAGGACAAGGCATGCTTAGCATCGGCTATGCCGTCTACCAGGTTCCCAAG
GAGCTGGAGAGTCACACGGACGCACACTTGGGCCGGGATTTCTTCCTGGCCTACCAGCCC
TCAGCCCGCACCAGCACCTACGTCAACCTGCGGGAGGTCTCTGGCCGGGCCCGGCTGCCC
CCTGGGGAGTACCTGGTGGTGCCATCCACATTTGAACCCTTCAAAGACGGCGAGTTCTGC
TTGAGAGTGTTCTCAGAGAAGAAGGCCCAGGCCCTAGAAATTGGGGATGTGGTAGCTGGA
AACCCATATGAGCCACATCCCAGTGAGGTGGATCAGGAAGATGACCAGTTCAGGAGGCTG
TTTGAGAAGTTGGCAGGGAAGGATTCTGAGATTACTGCCAATGCACTCAAGATACTTTTG
AATGAGGCGTTTTCCAAGAGAACAGACATAAAATTCGATGGATTCAACATCAACACTTGC
AGGGAAATGATCAGTCTGTTGGATAGCAATGGAATGGGCACTTTGGGGGCGGTGGAATTC
AAGACGCTCTGGCTGAAGATTCAGAAGTATCTGGAGATCTATTGGGAAACTGATTATAAC
CACTCGGGCACCATCGATGCCCACGAGATGAGGACAGCCCTCAGGAAGGCAGGTTTCACC
CTCAACAGCCAGGTGCAGCAGACCATTGCCCTGCGGTATGCGTGCAGCAAGCTTGGCATC
AACTTTGACAGCTTCGTGGCTTGTATGATCCGCCTGGAGACCCTCTTCAAACTATTCAGC
CTTCTGGACGAAGACAAGGATGGCATGGTTCAGCTCTCTCTGGCCGAGTGGCTGTGCTGC
GTGTTGGTC


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CAPN8
Symbol Nomenclature : CAPN8
SYNONYM : nCL-2
Designation : calpain-8|new calpain 2|stomach-specific M-type calpain
Full Nomenclature : calpain 8
GENEID : 388743
Locus Tag : RP11-105I12.2
HGNC : 1485
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00516210        ATGGCAGCCCAGGCAGCTGGTGTATCTAGGCAGCGGGCAGCCACTCAAGGTCTTGGCTCC
NM_001143962.1      ATGGCAGCCCAGGCAGCTGGTGTATCTAGGCAGCGGGCAGCCACTCAAGGTCTTGGCTCC
                    ************************************************************

HsCD00516210        AACCAAAACGCTTTGAAGTACTTGGGCCAGGATTTCAAGACCCTGAGGCAACAGTGCTTG
NM_001143962.1      AACCAAAACGCTTTGAAGTACTTGGGCCAGGATTTCAAGACCCTGAGGCAACAGTGCTTG
                    ************************************************************

HsCD00516210        GACTCAGGGGTCCTATTTAAGGACCCTGAGTTCCCAGCATGTCCATCAGCTTTGGGCTAC
NM_001143962.1      GACTCAGGGGTCCTATTTAAGGACCCTGAGTTCCCAGCATGTCCATCAGCTTTGGGCTAC
                    ************************************************************

HsCD00516210        AAGGATCTTGGACCAGGCTCTCCGCAAACTCAAGGCATCATCTGGAAGCGGCCCACGGAG
NM_001143962.1      AAGGATCTTGGACCAGGCTCTCCGCAAACTCAAGGCATCATCTGGAAGCGGCCCACGGAG
                    ************************************************************

HsCD00516210        TTGTGTCCCAGCCCTCAGTTTATCGTTGGTGGAGCCACGCGCACAGACATTTGTCAGGGT
NM_001143962.1      TTGTGTCCCAGCCCTCAGTTTATCGTTGGTGGAGCCACGCGCACAGACATTTGTCAGGGT
                    ************************************************************

HsCD00516210        GGTCTAGGTGACTGCTGGCTTCTGGCTGCCATTGCCTCCCTGACCCTGAATGAAGAGCTG
NM_001143962.1      GGTCTAGGTGACTGCTGGCTTCTGGCTGCCATTGCCTCCCTGACCCTGAATGAAGAGCTG
                    ************************************************************

HsCD00516210        CTTTACCGGGTGGTCCCCAGGGACCAGGACTTCCAGGAGAACTATGCGGGAATCTTTCAC
NM_001143962.1      CTTTACCGGGTGGTCCCCAGGGACCAGGACTTCCAGGAGAACTATGCGGGAATCTTTCAC
                    ************************************************************

HsCD00516210        TTTCAGTTCTGGCAGTACGGAGAGTGGGTGGAGGTGGTCATTGACGACAGGCTGCCCACC
NM_001143962.1      TTTCAGTTCTGGCAGTACGGAGAGTGGGTGGAGGTGGTCATTGACGACAGGCTGCCCACC
                    ************************************************************

HsCD00516210        AAGAATGGACAGCTGCTCTTCCTACACTCGGAACAAGGCAATGAATTCTGGAGTGCCCTG
NM_001143962.1      AAGAATGGACAGCTGCTCTTCCTACACTCGGAACAAGGCAATGAATTCTGGAGTGCCCTG
                    ************************************************************

HsCD00516210        CTGGAGAAAGCCTATGCCAAGCTTAATGGTTGTTATGAGGCTCTCGCTGGAGGTTCCACA
NM_001143962.1      CTGGAGAAAGCCTATGCCAAGCTTAATGGTTGTTATGAGGCTCTCGCTGGAGGTTCCACA
                    ************************************************************

HsCD00516210        GTGGAGGGGTTTGAGGATTTCACAGGTGGCATCTCTGAGTTTTATGACCTGAAGAAACCA
NM_001143962.1      GTGGAGGGGTTTGAGGATTTCACAGGTGGCATCTCTGAGTTTTATGACCTGAAGAAACCA
                    ************************************************************

HsCD00516210        CCAGCCAATCTATATCAGATCATCCGGAAGGCCCTCTGTGCGGGGTCTCTGCTGGGCTGC
NM_001143962.1      CCAGCCAATCTATATCAGATCATCCGGAAGGCCCTCTGTGCGGGGTCTCTGCTGGGCTGC
                    ************************************************************

HsCD00516210        TCCATTGATGTCTACAGTGCAGCCGAAGCCGAAGCCATCACCAGCCAGAAGCTGGTTAAG
NM_001143962.1      TCCATTGATGTCTCCAGTGCAGCCGAAGCCGAAGCCATCACCAGCCAGAAGCTGGTTAAG
                    *************.**********************************************

HsCD00516210        AGTCATGCGTACTCTGTCACTGGAGTCGAAGAGGTGAATTTCCAGGGCCATCCAGAGAAG
NM_001143962.1      AGTCATGCGTACTCTGTCACTGGAGTCGAAGAGGTGAATTTCCAGGGCCATCCAGAGAAG
                    ************************************************************

HsCD00516210        CTGATCAGACTCAGGAATCCATGGGGTGAAGTGGAGTGGTCGGGAGCCTGGAGCGATGAT
NM_001143962.1      CTGATCAGACTCAGGAATCCATGGGGTGAAGTGGAGTGGTCGGGAGCCTGGAGCGATGAT
                    ************************************************************

HsCD00516210        GCACCAGAGTGGAATCACATAGACCCCCGGCGGAAGGAAGAACTGGACAAGAAAGTTGAG
NM_001143962.1      GCACCAGAGTGGAATCACATAGACCCCCGGCGGAAGGAAGAACTGGACAAGAAAGTTGAG
                    ************************************************************

HsCD00516210        GATGGAGAATTCTGGATGTCACTTTCAGATTTCGTGAGGCAGTTCTCTCGGTTGGAGATC
NM_001143962.1      GATGGAGAATTCTGGATGTCACTTTCAGATTTCGTGAGGCAGTTCTCTCGGTTGGAGATC
                    ************************************************************

HsCD00516210        TGCAACCTGTCCCCGGACTCTCTGAGTAGCGAGGAGGTGCACAAATGGAACCTGGTCCTG
NM_001143962.1      TGCAACCTGTCCCCGGACTCTCTGAGTAGCGAGGAGGTGCACAAATGGAACCTGGTCCTG
                    ************************************************************

HsCD00516210        TTCAACGGCCACTGGACCCGGGGCTCCACAGCTGGGGGCTGCCAGAACTACCCAGCCACG
NM_001143962.1      TTCAACGGCCACTGGACCCGGGGCTCCACAGCTGGGGGCTGCCAGAACTACCCAGCCACG
                    ************************************************************

HsCD00516210        TACTGGACCAATCCCCAGTTCAAAATCCGTTTGGATGAAGTGGATGAGGACCAGGAGGAG
NM_001143962.1      TACTGGACCAATCCCCAGTTCAAAATCCGTTTGGATGAAGTGGATGAGGACCAGGAGGAG
                    ************************************************************

HsCD00516210        AGCATCGGTGAACCCTGCTGTACAGTGCTGCTGGGCCTGATGCAGAAAAATCGCAGGTGG
NM_001143962.1      AGCATCGGTGAACCCTGCTGTACAGTGCTGCTGGGCCTGATGCAGAAAAATCGCAGGTGG
                    ************************************************************

HsCD00516210        CGGAAGCGGATAGGACAAGGCATGCTTAGCATCGGCTATGCCGTCTACCAGGTTCCCAAG
NM_001143962.1      CGGAAGCGGATAGGACAAGGCATGCTTAGCATCGGCTATGCCGTCTACCAGGTTCCCAAG
                    ************************************************************

HsCD00516210        GAGCTGGAGAGTCACACGGACGCACACTTGGGCCGGGATTTCTTCCTGGCCTACCAGCCC
NM_001143962.1      GAGCTGGAGAGTCACACGGACGCACACTTGGGCCGGGATTTCTTCCTGGCCTACCAGCCC
                    ************************************************************

HsCD00516210        TCAGCCCGCACCAGCACCTACGTCAACCTGCGGGAGGTCTCTGGCCGGGCCCGGCTGCCC
NM_001143962.1      TCAGCCCGCACCAGCACCTACGTCAACCTGCGGGAGGTCTCTGGCCGGGCCCGGCTGCCC
                    ************************************************************

HsCD00516210        CCTGGGGAGTACCTGGTGGTGCCATCCACATTTGAACCCTTCAAAGACGGCGAGTTCTGC
NM_001143962.1      CCTGGGGAGTACCTGGTGGTGCCATCCACATTTGAACCCTTCAAAGACGGCGAGTTCTGC
                    ************************************************************

HsCD00516210        TTGAGAGTGTTCTCAGAGAAGAAGGCCCAGGCCCTAGAAATTGGGGATGTGGTAGCTGGA
NM_001143962.1      TTGAGAGTGTTCTCAGAGAAGAAGGCCCAGGCCCTAGAAATTGGGGATGTGGTAGCTGGA
                    ************************************************************

HsCD00516210        AACCCATATGAGCCACATCCCAGTGAGGTGGATCAGGAAGATGACCAGTTCAGGAGGCTG
NM_001143962.1      AACCCATATGAGCCACATCCCAGTGAGGTGGATCAGGAAGATGACCAGTTCAGGAGGCTG
                    ************************************************************

HsCD00516210        TTTGAGAAGTTGGCAGGGAAGGATTCTGAGATTACTGCCAATGCACTCAAGATACTTTTG
NM_001143962.1      TTTGAGAAGTTGGCAGGGAAGGATTCTGAGATTACTGCCAATGCACTCAAGATACTTTTG
                    ************************************************************

HsCD00516210        AATGAGGCGTTTTCCAAGAGAACAGACATAAAATTCGATGGATTCAACATCAACACTTGC
NM_001143962.1      AATGAGGCGTTTTCCAAGAGAACAGACATAAAATTCGATGGATTCAACATCAACACTTGC
                    ************************************************************

HsCD00516210        AGGGAAATGATCAGTCTGTTGGATAGCAATGGAATGGGCACTTTGGGGGCGGTGGAATTC
NM_001143962.1      AGGGAAATGATCAGTCTGTTGGATAGCAATGGAACGGGCACTTTGGGGGCGGTGGAATTC
                    ********************************** *************************

HsCD00516210        AAGACGCTCTGGCTGAAGATTCAGAAGTATCTGGAGATCTATTGGGAAACTGATTATAAC
NM_001143962.1      AAGACGCTCTGGCTGAAGATTCAGAAGTATCTGGAGATCTATTGGGAAACTGATTATAAC
                    ************************************************************

HsCD00516210        CACTCGGGCACCATCGATGCCCACGAGATGAGGACAGCCCTCAGGAAGGCAGGTTTCACC
NM_001143962.1      CACTCGGGCACCATCGATGCCCACGAGATGAGGACAGCCCTCAGGAAGGCAGGTTTCACC
                    ************************************************************

HsCD00516210        CTCAACAGCCAGGTGCAGCAGACCATTGCCCTGCGGTATGCGTGCAGCAAGCTTGGCATC
NM_001143962.1      CTCAACAGCCAGGTGCAGCAGACCATTGCCCTGCGGTATGCGTGCAGCAAGCTCGGCATC
                    ***************************************************** ******

HsCD00516210        AACTTTGACAGCTTCGTGGCTTGTATGATCCGCCTGGAGACCCTCTTCAAACTATTCAGC
NM_001143962.1      AACTTTGACAGCTTCGTGGCTTGTATGATCCGCCTGGAGACCCTCTTCAAACTATTCAGC
                    ************************************************************

HsCD00516210        CTTCTGGACGAAGACAAGGATGGCATGGTTCAGCTCTCTCTGGCCGAGTGGCTGTGCTGC
NM_001143962.1      CTTCTGGACGAAGACAAGGATGGCATGGTTCAGCTCTCTCTGGCCGAGTGGCTGTGCTGC
                    ************************************************************

HsCD00516210        GTGTTGGTC---
NM_001143962.1      GTGTTGGTCTGA
                    *********   

Clone

Cloning Information : 666180
Original Clone ID : ccsbBroadEn_10096

Organism


TAX_ID : 9606
Species Specific ID: 388743

Genome

Chromosome : 1
Map Location : 1q41

Reagents

Labome : CAPN8-antibody