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C14orf166B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  C14orf166B
Gene Name:  chromosome 14 open reading frame 166B
Sequence             
Original Clone ID: ccsbBroadEn_13228
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pENTR223               Format:  FUSION
Source: The ORFeome Collaboration
Description: Original GenBank Accession: BC121159.2
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
Harvard Medical School
HsCD00516461

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1387nts         Open reading frame : 1 to 1387
Reference Sequence Alignment

ATGCCCCCAGATGAGATTGAAATTGAGCCAGTACGACAGAGCAGCGATAAAATGCTCTAC
TGTGAGGCCGAATCCCCGCCGACTGTTGAAAAAGTGAAACCAGCCCGGGAGAATTCGGAA
ACAGACCTGGAGATTGAAGATGATGAGAAATTTTTCACCACTGGACAAAAGGAGCTGTAC
CTGGAGGCCTGCAAGCTGATGGGTGTAGTGCCTGTCTCCTACTTCATTCGGAACATGGAG
GAGTCCTACGTGAACCTCAACCACCACGGCCTGGGCCCCAGGGGTACCAAGGCTATTGCT
ATAGCCCTGGTGTCCAACATGGCTGTTACCAAACTGGAGCTGGAAGACAATTGCATCATG
GAGGAGGGCGTCTTGAGCCTGGTGGAGATGCTACAAGAGAACTACTACCTCCAGGAGATG
AATATTTCCAACAATCACCTTGGTTTGGAGGGGGCCAGAATCATCTCAGATTTCTTTGAG
AGAAACAGTTCTTCTATCTGGAGCCTTGAGCTTTCAGGAAATGACTTCAAGGAAGACTCC
GCAGCACTGCTCTGCCAAGCCCTGTCGACCAATTACCAAATTAAAAAGCTGGATCTCAGT
CACAACCAATTCTCTGATGTAGGAGGGGAGCACCTGGGCCAGATGCTGGCCATCAACGTG
GGGCTCACGTCACTGGATCTGAGCTGGAATAACTTCCACACAAGGGGAGCTGTGGCCTTG
TGCAATGGTCTCCGGGGTAATGTGACCCTGACAAAGCTGGATCTCTCCATGAATGGCTTT
GGGAATGAGGTGGCTCTGGCCCTAGGGGAAGTCCTCCGACTCAACCGCTGCCTGGTCTAC
CTGGATATCGGTGGCAATGACATCGGCAATGAAGGGGCCTCCAAAATCAGCAAAGGACTG
GAATCCAATGAAAGCCTCAGAGTTCTGAAGCTTTTCCTGAATCCCATAAATATGGATGGG
GCTATTTTACTTATCCTGGCTATCAAGAGGAACCCCAAATCCAGGATGGAAGAGCTTGAT
ATTTCCAACGTGCTGGTGTCCGAGCAGTTCATGAAAACGTTGGACGGAGTGTATGCCGTT
CACCCGCAGCTGGACGTGGTATTCAAGGCAGTACAAGGCCTCTCTCCCAAGAAAACCATC
TTCTTGTTGACAAACCCCATGAAACTGATCCAGAGCTATGCAGACCAACACAAAATCACG
ATCGTGGACTTCTTCAAGAGCTTGAACCCTACTGGGACAATGAAGATGTCTGTGGATGAG
TTCCAGAAAGTGATGATAGAGCAAAACAAGGTCCCCCTGAACCAGTACCAGGTCAGGGAG
GTGATAAAGAAGCTCGATGAGAAGACAGGCATGGTGAACTTCAGTTTCTTGAACACGATG
AAGCCA


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pENTR223

pENTR223 in advanced viewed

Synonyms: pDONR223, pENTR223-XnCDSYn
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp): 2815
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Mark Vidal
Publications: PMID: 15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication 2220 2753
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : C14orf166B
Symbol Nomenclature : C14orf166B
Designation : uncharacterized protein C14orf166B
Full Nomenclature : chromosome 14 open reading frame 166B
GENEID : 145497
HGNC : 23346
Vega : OTTHUMG00000150153
Target GenBank: None

 

Reference Sequence Alignment

  

HsCD00516461      ------------------------------------------------------------
NM_194287.2       ATGCACATCCAATTCCCATCAAAGCCTACTCTTCCCAGGGCTTGCTGGGAGGGAAGGATA
                                                                              

HsCD00516461      ------------------ATGCCCCCAGATGAGATTGAAATTGAGCCAGTACGACAGAGC
NM_194287.2       ACTGCAGGCTCCCCTGGGATGCCCCCAGATGAGATTGAAATTGAGCCAGTACGACAGAGC
                                    ******************************************

HsCD00516461      AGCGATAAAATGCTCTACTGTGAGGCCGAATCCCCGCCGACTGTTGAAAAAGTGAAACCA
NM_194287.2       AGCGATAAAATGCTCTACTGTGAGGCCGAATCCCCGCCGACTGTTGAAAAAGTGAAACCA
                  ************************************************************

HsCD00516461      GCCCGGGAGAATTCGGAAACAGACCTGGAGATTGAAGATGATGAGAAATTTTTCACCACT
NM_194287.2       GCCCGGGAGAATTCGGAAACAGACCTGGAGATTGAAGATGATGAGAAATTTTTCACCACT
                  ************************************************************

HsCD00516461      GGACAAAAGGAGCTGTACCTGGAGGCCTGCAAGCTGATGGGTGTAGTGCCTGTCTCCTAC
NM_194287.2       GGACAAAAGGAGCTGTACCTGGAGGCCTGCAAGCTGATGGGTGTAGTGCCTGTCTCCTAC
                  ************************************************************

HsCD00516461      TTCATTCGGAACATGGAGGAGTCCTACGTGAACCTCAACCACCACGGCCTGGGCCCCAGG
NM_194287.2       TTCATTCGGAACATGGAGGAGTCCTACGTGAACCTCAACCACCACGGCCTGGGCCCCAGG
                  ************************************************************

HsCD00516461      GGTACCAAGGCTATTGCTATAGCCCTGGTGTCCAACATGGCTGTTACCAAACTGGAGCTG
NM_194287.2       GGTACCAAGGCTATTGCTATAGCCCTGGTGTCCAACATGGCTGTTACCAAACTGGAGCTG
                  ************************************************************

HsCD00516461      GAAGACAATTGCATCATGGAGGAGGGCGTCTTGAGCCTGGTGGAGATGCTACAAGAGAAC
NM_194287.2       GAAGACAATTGCATCATGGAGGAGGGCGTCTTGAGCCTGGTGGAGATGCTACAAGAGAAC
                  ************************************************************

HsCD00516461      TACTACCTCCAGGAGATGAATATTTCCAACAATCACCTTGGTTTGGAGGGGGCCAGAATC
NM_194287.2       TACTACCTCCAGGAGATGAATATTTCCAACAATCACCTTGGTTTGGAGGGGGCCAGAATC
                  ************************************************************

HsCD00516461      ATCTCAGATTTCTTTGAGAGAAACAGTTCTTCTATCTGGAGCCTTGAGCTTTCAGGAAAT
NM_194287.2       ATCTCAGATTTCTTTGAGAGAAACAGTTCTTCTATCTGGAGCCTTGAGCTTTCAGGAAAT
                  ************************************************************

HsCD00516461      GACTTCAAGGAAGACTCCGCAGCACTGCTCTGCCAAGCCCTGTCGACCAATTACCAAATT
NM_194287.2       GACTTCAAGGAAGACTCCGCAGCACTGCTCTGCCAAGCCCTGTCGACCAATTACCAAATT
                  ************************************************************

HsCD00516461      AAAAAGCTGGATCTCAGTCACAACCAATTCTCTGATGTAGGAGGGGAGCACCTGGGCCAG
NM_194287.2       AAAAAGCTGGATCTCAGTCACAACCAATTCTCTGATGTAGGAGGGGAGCACCTGGGCCAG
                  ************************************************************

HsCD00516461      ATGCTGGCCATCAACGTGGGGCTCACGTCACTGGATCTGAGCTGGAATAACTTCCACACA
NM_194287.2       ATGCTGGCCATCAACGTGGGGCTCACGTCACTGGATCTGAGCTGGAATAACTTCCACACA
                  ************************************************************

HsCD00516461      AGGGGAGCTGTGGCCTTGTGCAATGGTCTCCGGGGTAATGTGACCCTGACAAAGCTGGAT
NM_194287.2       AGGGGAGCTGTGGCCTTGTGCAATGGTCTCCGGGGTAATGTGACCCTGACAAAGCTGGAT
                  ************************************************************

HsCD00516461      CTCTCCATGAATGGCTTTGGGAATGAGGTGGCTCTGGCCCTAGGGGAAGTCCTCCGACTC
NM_194287.2       CTCTCCATGAATGGCTTTGGGAATGAGGTGGCTCTGGCCCTAGGGGAAGTCCTCCGACTC
                  ************************************************************

HsCD00516461      AACCGCTGCCTGGTCTACCTGGATATCGGTGGCAATGACATCGGCAATGAAGGGGCCTCC
NM_194287.2       AACCGCTGCCTGGTCTACCTGGATATCGGTGGCAATGACATCGGCAATGAAGGGGCCTCC
                  ************************************************************

HsCD00516461      AAAATCAGCAAAGGACTGGAATCCAATGAAAGCCTCAGAGTTCTGAAGCTTTTCCTGAAT
NM_194287.2       AAAATCAGCAAAGGACTGGAATCCAATGAAAGCCTCAGAGTTCTGAAGCTTTTCCTGAAT
                  ************************************************************

HsCD00516461      CCCATAAATATGGATGGGGCTATTTTACTTATCCTGGCTATCAAGAGGAACCCCAAATCC
NM_194287.2       CCCATAAATATGGATGGGGCTATTTTACTTATCCTGGCTATCAAGAGGAACCCCAAATCC
                  ************************************************************

HsCD00516461      AGGATGGAAGAGCTTGATATTTCCAACGTGCTGGTGTCCGAGCAGTTCATGAAAACGTTG
NM_194287.2       AGGATGGAAGAGCTTGATATTTCCAACGTGCTGGTGTCCGAGCAGTTCATGAAAACGTTG
                  ************************************************************

HsCD00516461      GACGGAGTGTATGCCGTTCACCCGCAGCTGGACGTGGTATTCAAGGCAGTACAAGGCCTC
NM_194287.2       GACGGAGTGTATGCCGTTCACCCGCAGCTGGACGTGGTATTCAAGGCAGTACAAGGCCTC
                  ************************************************************

HsCD00516461      TCTCCCAAGAAAACCATCTTCTTGTTGACAAACCCCATGAAACTGATCCAGAGCTATGCA
NM_194287.2       TCTCCCAAGAAAACCATCTTCTTGTTGACAAACCCCATGAAACTGATCCAGAGCTATGCA
                  ************************************************************

HsCD00516461      GACCAACACAAAATCACGATCGTGGACTTCTTCAAGAGCTTGAACCCTACTGGGACAATG
NM_194287.2       GACCAACACAAAATCACGATCGTGGACTTCTTCAAGAGCTTGAACCCCACTGGGACAATG
                  *********************************************** ************

HsCD00516461      AAGATGTCTGTGGATGAGTTCCAGAAAGTGATGATAGAGCAAAACAAGGTCCCCCTGAAC
NM_194287.2       AAGATGTCTGTGGATGAGTTCCAGAAAGTGATGATAGAGCAAAACAAGGTCCCCCTGAAC
                  ************************************************************

HsCD00516461      CAGTACCAGGTCAGGGAGGTGATAAAGAAGCTCGATGAGAAGACAGGCATGGTGAACTTC
NM_194287.2       CAGTACCAGGTCAGGGAGGTGATAAAGAAGCTCGATGAGAAGACAGGCATGGTGAACTTC
                  ************************************************************

HsCD00516461      AGTTTCTTGAACACGATGAAGCCA---
NM_194287.2       AGTTTCTTGAACACGATGAAGCCATAG
                  ************************   

Clone

Cloning Information : 669191
Original Clone ID : ccsbBroadEn_13228

Organism


TAX_ID : 9606
Species Specific ID: 145497

Genome

Chromosome : 14
Map Location : 14q24.3
Ensembl : ENSG00000100565

Reagents

Labome : C14orf166B-antibody