DNASU Plasmid | gld-2 (C. elegans )<br>In pEU-His-FV (GST-tagged cell-free expression vector)

Explanation of Terms

Gene:

Gene Symbol: gld-2

Gene Name: Protein GLD-2

Original Clone ID:

5962.1.19_GO.102088

Keyword:

partial cds

Species:

Caenorhabditis elegans

Type:

cDNA

Vector Name:

pEU-His-FV

Format:

CLOSED

Source:

Center for Eukaryotic Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

PMID: 19460348
Title: Antagonism between GLD-2 binding partners controls gamete sex.
PMID: 17012378
Title: The GLD-2 poly(A) polymerase activates gld-1 mRNA in the Caenorhabditis elegans germ line.
PMID: 16000383
Title: Dose-dependent control of proliferation and sperm specification by FOG-1/CPEB.
PMID: 19221201
Title: FBF and its dual control of gld-1 expression in the Caenorhabditis elegans germline.
PMID: 12239571
Title: A regulatory cytoplasmic poly(A) polymerase in Caenorhabditis elegans.

Authors:

University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics

CeCD00519087

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1782nts

Open reading frame: 17 to 1798

TCATCATCATCATCACGCGATCGCCGAAAACCTGTACTTCCAGTCCTCGCCATCTCCACC
TACATCTCTTCTTTCCGAGCCACTCTCTAGGATGGACGTTCTCAGCGAGAAAATTTGGGA
TTATCACAACAAGGTGTCTCAAACTGACGAAATGCTCCAAAGAAAGCTACATCTTCGTGA
TATGCTCTATACTGCAATCAGTCCTGTTTTCCCACTATCCGGACTATACGTTGTCGGTTC
ATCACTTAATGGTTTTGGTAACAACTCATCTGACATGGATCTTTGCCTGATGATCACTAA
CAAAGATCTGGATCAGAAGAACGACGCAGTTGTCGTTCTAAATCTCATACTTTCCACTTT
GCAATACGAGAAATTTGTGGAAAGTCAGAAGCTCATTTTGGCAAAAGTTCCCATTCTTCG
GATAAACTTTGCTGCACCTTTTGATGATATAACTGTTGATCTGAATGCGAATAATTCGGT
TGCTATTAGAAATACCCATTTATTGTGCTACTATTCGTCATATGACTGGCGAGTACGGCC
GTTGGTATCAGTTGTGAAGGAGTGGGCAAAACGGAAAGGGATTAACGATGCAAACAAATC
GAGTTTTACAAGTTATTCTCTTGTTTTAATGGTTATTCACTTCTTGCAATGCGGCCCGAC
AAAAGTTTTACCAAATTTGCAACAATCTTATCCAAATCGATTCTCGAACAAAGTTGATGT
TCGCACATTGAATGTTACGATGGCACTTGAAGAAGTAGCTGATGATATTGATCAATCACT
TTCTGAAAAGACGACACTCGGAGAGCTTCTTATTGGATTCCTTGATTATTACGCAAATGA
GTTCAATTATGATCGTGATGCCATCTCGATTCGGCAAGGAAGACGTGTAGAAAGGGCTGC
ACTTGCTGTTCGTCCAAAAATACATTCGAATAGTGAAGGAGACAAAGAGACACCTCCACC
ATCATCATCTGCATCGACTTCTTCCATCCACAATGGAGGTACTCCAGGAATCCCAATGCA
TCATTCCATTTCAAATCCCCACTTCTGGCGGTCTCAATGGCGTTGCGTGTGTATAGAAGA
ACCGTTCACTAATTCGAATACAGCACACTCGATATATGACGAAATGGTATTTGAAGCAAT
CAAAAAAGCATTCCGTGAAGCTCATGGAGAGTTGCAACATAATCATGATTTGGATAAATT
GATGGAGTGTGAACCTATAAAGGCATCCACAACCAACACAGGAGCTGCTGTCTTTGCCGC
CACCTATGAAGGTGAACGTCCTTTGGCTCAACAACCTAATACGATTGCGTGTGCTAGTCT
TCGTGTCTTGAATTCTATTCCTGTTTCATCTGGTCCAGGACACTATCACTACCAACAACA
ATCCAATCAGAATCTGTCACGACCACAACGACCAGGAAGTAACCAAGGATACCAAATGAA
TAATAATCGTGGGTTCAACGGCAACAATCAGCAGCAACATCAAAATCGGCGATCATTCAA
CAATCAGTCATCATCGAATCCAGGAAACGGATCTACTGGACCACGATCTTCAAGATCTAA
TGAAAACGTCAGAGACTCGTCACGTCAACAGAACAGTCAGAAAGGAAGTTCGGGAGTGTC
TGTTTCTAAGGAGAACGTGGCAAGTACGACCGGAGTCCCAGTAGACAAGAAGCAGCAAAA
TTCAAATAGGAAAGACGACGGAAATAGAACGAAACGGTCACCGATGGTGCAATCACCAGA
ACCAGCTAAAACAAAGTCGGAAAAAACTCCGATGGCATCATCAAATGTATCTCAATGATA
GTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCT

Protein sequence predicted based on nucleotide sequence

AIAENLYFQSSPSPPTSLLSEPLSRMDVLSEKIWDYHNKVSQTDEMLQRKLHLRDMLYTA
ISPVFPLSGLYVVGSSLNGFGNNSSDMDLCLMITNKDLDQKNDAVVVLNLILSTLQYEKF
VESQKLILAKVPILRINFAAPFDDITVDLNANNSVAIRNTHLLCYYSSYDWRVRPLVSVV
KEWAKRKGINDANKSSFTSYSLVLMVIHFLQCGPTKVLPNLQQSYPNRFSNKVDVRTLNV
TMALEEVADDIDQSLSEKTTLGELLIGFLDYYANEFNYDRDAISIRQGRRVERAALAVRP
KIHSNSEGDKETPPPSSSASTSSIHNGGTPGIPMHHSISNPHFWRSQWRCVCIEEPFTNS
NTAHSIYDEMVFEAIKKAFREAHGELQHNHDLDKLMECEPIKASTTNTGAAVFAATYEGE
RPLAQQPNTIACASLRVLNSIPVSSGPGHYHYQQQSNQNLSRPQRPGSNQGYQMNNNRGF
NGNNQQQHQNRRSFNNQSSSNPGNGSTGPRSSRSNENVRDSSRQQNSQKGSSGVSVSKEN
VASTTGVPVDKKQQNSNRKDDGNRTKRSPMVQSPEPAKTKSEKTPMASSNVSQ*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in GO.102088

Recommended expression in:

Not Applicable

Vector Name:

pEU-His-FV

Synonyms:

Sequencing Primer:

Forward: pEU319 Forward

Reverse: pEU Reverse

Description:

Cell-free expression vector, adds N-terminal 6xHis tag; amp resistance; restriction enzyme cloning (SgfI, PmeI).

Comments:

Cloning into this vector will remove the BarCat casstte. To avoid, the toxicity of the barnase gene, the vector pEU-HSBC (EvNO00306667) can be ordered, which substitutes sacB for barnase.

Size (bp):

4966

Parent Vector:

None

Properties:

cell-free expression, multiple cloning site, with tag/fusion/marker

Author Name:

Center for Eukaryotic Structural Genomics

Publications:

PMID: 15782178

Title: Cell-free protein production and labeling protocol for NMR-based structural proteomics

Vector Map:

Vector Sequence:

Sequence:

CACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCTGGCTAGCGATGACCCTGCTGATTGGTTCGCTGACCATTTCCGGGTGCGGGACGGCGTTACCAGAAACTCAGAAGGTTCGTCCAACCAAACCGACTCTGGCGGCAGTTTACGAGAGAGATGATAGGGTCTGCTTCAGTAAGCCAGATGCTACACAATTAGGCTTGTACATACTGTCGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATACGATTTAGGTGACACTATAGAATACACGGAATTCGCGTTTTTATTTTTAATTTTCTTTCAAATACTTCTAGCTAGAGTATTTTTACAACAATTACCAACAACAACAACAAACAACAACAACATTACATTTTACATTCTACAACTACAGCCATGGGACATCATCATCATCATCACGCGATCGCCATGGAATAAGTAAAGGAATCACATGGCACAGGTTATCAACACGTTTGACGGGGTTGCGGATTATCTTCAGACATATCATAAGCTACCTGATAATTACATTACAAAATCAGAAGCACAAGCCCTCGGCTGGGTGGCATCAAAAGGGAACCTTGCAGACGTCGCTCCGGGGAAAAGCATCGGCGGAGACATCTTCTCAAACAGGGAAGGCAAACTCCCGGGCAAAAGCGGACGAACATGGCGTGAAGCGGATATTAACTATACATCAGGCTTCAGAAATTCAGACCGGATTCTTTACTCAAGCGACTGGCTGATTTACAAAACAACGGACCATTATCAGACCTTTACAAAAATCAGATAATTAGGCACCCCAGGCTTTACACTTTATGCTTTCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATGTTTAAACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAAAAAACTAGTTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTATATCAGGCTGTGTTTAAGACGCCGCCGCTTCGCCCAAATCCCTATGCCGGTTCGACGGCTGGACAAAATACTGTTTATCTTCCCAGCGCAGGCAGGTTAATGTACCACCCCAGCAGCAGCCGGTATCCAGCGCGTATATACCTTCCGGCGTACCTTTGCCCTCCAGCGATGCCCAGTGACCAAAGGCGATGCTGTATTCTTCAGCGACAGGGCCAGGAATCGCAAACCACGGTTTCAGTGGGGCAGGGGCCTCTTCCGGCGATTCTTACTAGCTAGTATGCATAGGTGCTGAAATATAAAGTTTGTGTTTCTAAAACACACGTGGTACGTACGATAACGTACAGTGTTTTTCCCTCCACTTAAATCGAAGGGTAGTGTCTTGGAGCGCGCGGAGTAAACATATATGGTTCATATATGTCCGTAGGCACGTAAAAAAAGCGAGGGATTCGAATTCCCCCGGAACCCCCGGTTGGGGCCCACGCCTCGATCGAGCAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAGCTTTCCCGCGGCCAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCACTCACTGACTCGCTGCGCTCGGTCGCTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGCGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 origin

2809

3491

gene fragment

Omega

TMV Omega

397

520

gene fragment

TMV-L

278

2238

gene fragment

BarCat-bar

BarCat cassette, including Barnase

572

908

gene fragment

BarCat-cat

BarCat cassette, including CAT

1010

1667

primer

His2 forward

His2 forward sequencing primer TCTTTCAAATACTTCTAGCTAGAGTA

424

449

promoter

SP6

SP6 promoter

373

390

recombination site

MCS

pF1K (Flexi-Vector) homology domain

1675

1803

selectable marker

AmpR

ampicillin resistance gene

3589

4248

tag

His

6xHis tag

527

544

Gene

Gene Symbol :

gld-2

GENEID :

172338

Locus Tag :

CELE_ZC308.1

GI :

71996960

GenBank Accession :

NM_001026674

Target GenBank:

NM_001026674

Protein

Structural Annotation Wiki :

GO.102088

Clone

Cloning Information :

510513

HIP Master Clone ID :

421680

Original Clone ID :

5962.1.19_GO.102088

Experimental

TargetTrack - Experimental data :

GO.102088

Organism

Species Specific ID:

172338

Genome

Chromosome :

Reagents

Labome :

gld-2-cDNA

gld-2 (C. elegans )In pEU-His-FV (GST-tagged cell-free expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Experimental

Organism

Genome

Reagents

gld-2 (C. elegans )
In pEU-His-FV (GST-tagged cell-free expression vector)