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Taci_0264 (T. acidaminovorans DSM 6589 ) in pMCSG7 (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  Taci_0264
Gene Name:  PAS/PAC sensor protein
Sequence             
Original Clone ID: APC101351.102
Keyword: None
Species: Thermanaerovibrio acidaminovorans DSM 6589
Type: cDNA
Vector Name: pMCSG7               Format:  CLOSED
Source: Midwest Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Midwest Center for Structural Genomics
Jessica Bearden
PSI:Biology
TaCD00519985

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1362nts         Open reading frame : 46 to 1407

GGTGTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGAGGGAGGTGCGC
TTCAAGGTCCCCCTCATGTTCACCCTTCTCTTCCTGGCCCTGTCGGGGATTCTGTACCTT
TCACTGTTGGCCCGGGCGGACCGGGTCCTCTACCAGCTCGAGCTGTCCAACGGGATCAAG
GGGGGCGAGGCGGTCAGGAACTTCCTGGACCACCAGGCCCGGCAGCTGGACCTCCTCTGT
CTGGACTGGGCCAGCTGGGATCAGATGTACCACTTCGTTGAGGGGCGGATGGAGTCCTTC
GAGAGGCAGATCCTGAGCTACGAGAGCCTGGAGAACGCGGAGGTCTCCCACGGGGCGTTG
TTCCGCAGGGACCTCACGGTGGTCTGGCACGGGAAGCCCGAGGGGAGGATCAGGTCCGTG
GTGCCCTGCGCCCCGGAGGAGCTCACCAAGCTGCGGGAGACGGTGATCCGGGCGGAGAGG
TACGGGTACGCCCGGGGGCTCACGGTCATGTCGGACCGGATACTGATGGTATCCGCCAGG
CAGGTCAAGGACACCGCCATGGGGCTCCCGTACAATGGATGGCTTGTCCTGGCCAGGCCC
CTGGCCTCCGCCGGGGAGCTCCGGAGGGTGTTGCCCAGCCTGGTTGACGTCCGTCCCTCC
CAGGTGGCCATGGAGCCCAAGGTGGTCACCGTGCCGGTGAGCCCCTCGGTGATGGAGACC
CGGGTCCCCCTGATGGACATAACCGGCCGCTGGTCCATGGAGGCCCGGCTCCGGGTGGAT
CGGTGGATCGCCATGGGGCTTCGGGAGCTCATGGGGATGGTGCTGGTGGGGATCGCCATG
CTGGGGGTGAGCCTAGGCACCGCCATATTCCTATGGCACAGGACCAGCATATCCCAGCCG
TTGGAGGAGATGCGGCGCCGGATATCCCGAATGATCTCCGAGGGGGGCTTGAGGCCCCTG
GAGGCTCCATCGGAGGAGCTGGAGTCGGTGGTGGGGCTCGCCAACCTGCTGATAAGGCAC
TGCACCACCATGGAGCGGCGGCTCATAGGGGAGAACCGGCGGATCCGGGAGATCCTGGAC
GGGATGGACCTGTTGGTGGCCCTTTGCACCACCTCCGGGGACGTGACCTTCGCCAACCGG
GCCATGAGGGACCTCTTCGACTCCCTGGAGCCCCTGGTGGGCCGGAAGATATGGGAGATC
GGGGACGTGGAGGACCGGGAGAGGATCAGGGACCGGTTCCTGGCCACCGCCGAGGGGGCC
CCTCAGCTGATAAGGGTCCACCTGAGGGAGAAGCGCACGTCCATGGTGATGAACATGTCC
CACGTTCACCGGGTGGGAGGCGACGGGGAGGTGCTGGTCCTATGCAGGAGGTCCAGCGCT
CACATCCCCCCCGGCATCTTCGCCTAACATTGGAAGTGGATAATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Not_Tested
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in MCSG-APC101351
Recommended expression in: E.coli BL21 (DE3)
       DyNA Vector Map

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Vector Name: pMCSG7

pMCSG7 in advanced viewed

Synonyms: pMCSG-7
Sequencing Primer: Forward:  T7short
Reverse:  T7 terminator
Description: Bacterial expresson vector with T7 promoter, adds N-terminal His tag and TEV protease site; amp resistance; ligation independent cloning (LIC).
Comments: pET21 derivative; for routine HTP purification
Size (bp): 5286
Parent Vector: None
Empty Vector: EvNO00084271
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 12071693
Title: A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC ligation-independent cloning (LIC) region 204 235
bacterial origin ori ColE1 pBR322-type bacterial origin of replication 3280 3280
promoter T7 T7 promoter 360 376
protease cleavage site TEV TEV cleavage site cds 223 243
repressor binding site lac operator LacO fragment 335 357
repressor protein gene LacI LacI coding region 890 1849
selectable marker AmpR ampicillin resistance gene 4038 4898
tag His 2 N-terminal 6xHis tag 268 285
tag His C-terminal 6xHis tag 140 157
trxn termination sequence T7 term T7 terminator sequence 26 72

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : Taci_0264
GENEID : 8630074
Locus Tag : Taci_0264
GI : 269099514
Target GenBank: None

 

Protein

SMART domain : PAS : PAS domain
Structural Annotation Wiki : MCSG-APC101351
Structural Annotation Wiki : TOPSAN

Clone

Cloning Information : 584580
HIP Master Clone ID : 487509
Original Clone ID : APC101351.102

Experimental

TargetTrack - Experimental data : MCSG-APC101351

Organism


TAX_ID : 525903
Species Specific ID: 8630074

Reagents

Labome : Taci_0264-cDNA