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RAB1A (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

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Explanation of Terms


Gene: Gene Symbol:  RAB1A
Gene Name:  RAB1A, member RAS oncogene family
Sequence              Map: pANT7_cGST.doc
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  CLOSED
Source: Center for Personalized Diagnostics
Description: None
Comments: wrong FLEX/vector info
Mutation/
Discrepancy :
No / No
Publications: PMID: 24582959
Title: Selective Protection of an ARF1-GTP Signaling Axis by a Bacterial Scaffold Induces Bidirectional Trafficking Arrest
Authors: Center for Personalized Diagnostics
HsCD00520592

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UNAVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 618nts         Open reading frame : 179 to 796
Reference Sequence Alignment

ATGTCCAGCATGAATCCCGAATATGATTATTTATTCAAGTTACTTCTGATTGGCGACTCA
GGGGTTGGAAAGTCTTGCCTTCTTCTTAGGTTTGCAGATGATACATATACAGAAAGCTAC
ATCAGCACAATTGGTGTGGATTTCAAAATAAGAACTATAGAGTTAGACGGGAAAACAATC
AAGCTTCAAATATGGGACACAGCAGGCCAGGAAAGATTTCGAACAATCACCTCCAGTTAT
TACAGAGGAGCCCATGGCATCATAGTTGTGTATGATGTGACAGATCAGGAGTCCTTCAAT
AATGTTAAACAGTGGCTGCAGGAAATAGATCGTTATGCCAGTGAAAATGTCAACAAATTG
TTGGTAGGGAACAAATGTGATCTGACCACAAAGAAAGTAGTAGACTACACAACAGCGAAG
GAATTTGCTGATTCCCTTGGAATTCCGTTTTTGGAAACCAGTGCTAAGAATGCAACGAAT
GTAGAACAGTCTTTCATGACGATGGCAGCTGAGATTAAAAAGCGAATGGGTCCCGGAGCA
ACAGCTGGTGGTGCTGAGAAGTCCAATGTTAAAATTCAGAGCACTCCAGTCAAGCAGTCA
GGTGGAGGTTGCTGCTAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : RAB1A
Symbol Nomenclature : RAB1A
SYNONYM : RAB1; YPT1
Designation : GTP binding protein Rab1a|RAB1, member RAS oncogene family|Rab GTPase YPT1 homolog|YPT1-related protein|ras-related protein Rab-1A
Full Nomenclature : RAB1A, member RAS oncogene family
GENEID : 5861
GenBank Accession : BC000905
HGNC : 9758
MIM : 179508
Vega : OTTHUMG00000152725
Target GenBank: BC000905

 

Reference Sequence Alignment

  

HsCD00520592      ATGTCCAGCATGAATCCCGAATATGATTATTTATTCAAGTTACTTCTGATTGGCGACTCA
NM_004161.4       ATGTCCAGCATGAATCCCGAATATGATTATTTATTCAAGTTACTTCTGATTGGCGACTCA
NM_030981.2       ---------ATGAACCCCGAATATGACTACCTGTTTAAGCTGCTTTTGATTGGCGACTCA
                           ***** *********** **  *.** *** *.*** **************

HsCD00520592      GGGGTTGGAAAGTCTTGCCTTCTTCTTAGGTTTGCAGATGATACATATACAGAAAGCTAC
NM_004161.4       GGGGTTGGAAAGTCTTGCCTTCTTCTTAGGTTTGCAGATGATACATATACAGAAAGCTAC
NM_030981.2       GGCGTGGGCAAGTCATGCCTGCTCCTGCGGTTTGCTGATGACACGTACACAGAGAGCTAC
                  ** ** **.*****:***** ** ** .*******:***** **.** *****.******

HsCD00520592      ATCAGCACAATTGGTGTGGATTTCAAAATAAGAACTATAGAGTTAGACGGGAAAACAATC
NM_004161.4       ATCAGCACAATTGGTGTGGATTTCAAAATAAGAACTATAGAGTTAGACGGGAAAACAATC
NM_030981.2       ATCAGCACCATCGGGGTGGACTTCAAGATCCGAACCATCGAGCTGGATGGCAAAACTATC
                  ********.** ** ***** *****.**..**** **.*** *.** ** *****:***

HsCD00520592      AAGCTTCAAATATGGGACACAGCAGGCCAGGAAAGATTTCGAACAATCACCTCCAGTTAT
NM_004161.4       AAGCTTCAAATATGGGACACAGCAGGCCAGGAAAGATTTCGAACAATCACCTCCAGTTAT
NM_030981.2       AAACTTCAGATCTGGGACACAGCGGGCCAGGAACGGTTCCGGACCATCACTTCCAGCTAC
                  **.*****.**.***********.*********.*.** **.**.***** ***** ** 

HsCD00520592      TACAGAGGAGCCCATGGCATCATAGTTGTGTATGATGTGACAGATCAGGAGTCCTTCAAT
NM_004161.4       TACAGAGGAGCCCATGGCATCATAGTTGTGTATGATGTGACAGATCAGGAGTCCTTCAAT
NM_030981.2       TACCGGGGGGCTCATGGCATCATCGTGGTGTATGACGTCACTGACCAGGAATCCTACGCC
                  ***.*.**.** ***********.** ******** ** **:** *****.****:*.. 

HsCD00520592      AATGTTAAACAGTGGCTGCAGGAAATAGATCGTTATGCCAGTGAAAATGTCAACAAATTG
NM_004161.4       AATGTTAAACAGTGGCTGCAGGAAATAGATCGTTATGCCAGTGAAAATGTCAACAAATTG
NM_030981.2       AACGTGAAGCAGTGGCTGCAGGAGATTGACCGCTATGCCAGCGAGAACGTCAATAAGCTC
                  ** ** **.**************.**:** ** ******** **.** ***** **. * 

HsCD00520592      TTGGTAGGGAACAAATGTGATCTGACCACAAAGAAAGTAGTAGACTACACAACAGCGAAG
NM_004161.4       TTGGTAGGGAACAAATGTGATCTGACCACAAAGAAAGTAGTAGACTACACAACAGCGAAG
NM_030981.2       CTGGTGGGCAACAAGAGCGACCTCACCACCAAGAAGGTGGTGGACAACACCACAGCCAAG
                   ****.** *****.:* ** ** *****.*****.**.**.***:****.***** ***

HsCD00520592      GAATTTGCTGATTCCCTTGGAATTCCGTTTTTGGAAACCAGTGCTAAGAATGCAACGAAT
NM_004161.4       GAATTTGCTGATTCCCTTGGAATTCCGTTTTTGGAAACCAGTGCTAAGAATGCAACGAAT
NM_030981.2       GAGTTTGCAGACTCTCTGGGCATCCCCTTCTTGGAGACGAGCGCCAAGAATGCCACCAAT
                  **.*****:** ** ** **.** ** ** *****.** ** ** ********.** ***

HsCD00520592      GTAGAACAGTCTTTCATGACGATGGCAGCTGAGATTAAAAAGCGAATGGGTCCCGGAGCA
NM_004161.4       GTAGAACAGTCTTTCATGACGATGGCAGCTGAGATTAAAAAGCGAATGGGTCCCGGAGCA
NM_030981.2       GTCGAGCAGGCGTTCATGACCATGGCTGCTGAAATCAAAAAGCGGATGGGGCCTGGAGCA
                  **.**.*** * ******** *****:*****.** ********.***** ** ******

HsCD00520592      ACAGCTGGTGGTGCTGAGAAGTCCAATGTTAAAATTCAGAGCACTCCAGTCAAGCAGTCA
NM_004161.4       ACAGCTGGTGGTGCTGAGAAGTCCAATGTTAAAATTCAGAGCACTCCAGTCAAGCAGTCA
NM_030981.2       GCCTCTGGGGGCGAG---CGGCCCAATCTCAAGATCGACAGCACCCCTGTAAAGCCGGCT
                  .*. **** ** *.    ..* ***** * **.**  * ***** **:**.****.* *:

HsCD00520592      GGTGGAGGTTGCTGCTAA
NM_004161.4       GGTGGAGGTTGCTGCTAA
NM_030981.2       GGCGGTGGCTGTTGCTAG
                  ** **:** ** *****.

Pathways

NCI : PLK1 signaling events
Reactome : Cell Cycle
Reactome : Cell Cycle, Mitotic
Reactome : Golgi Cisternae Pericentriolar Stack Reorganization
Reactome : M Phase
Reactome : Mitotic M-M/G1 phases
Reactome : Mitotic Prophase

Clone

Cloning Information : 167548

Organism


TAX_ID : 9606
Species Specific ID: 5861

Genome

Chromosome : 2
Map Location : 2p14
Ensembl : ENSG00000138069

Reagents

Labome : RAB1A-antibody