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FRAT1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  FRAT1
Gene Name:  frequently rearranged in advanced T-cell lymphomas
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH135178.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00005253

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 840nts        
Open reading frame : 1 to 840
Reference Sequence Alignment

ATGCCGTGCCGGAGGGAGGAGGAAGAGGAAGCCGGCGAGGAGGCGGAGGGGGAGGAAGAG
GAGGAGGACAGCTTCCTCCTACTGCAGCAGTCAGTGGCGCTGGGCAGCTCGGGCGAGGTG
GACCGGCTGGTGGCCCAGATCGGCGAGACGCTGCAGCTGGACGCGGCGCAGCACAGCCCG
GCCTCGCCGTGCGGGCCCCCGGGGGCGCCGCTGCGGGCCCCGGGGCCCCTGGCTGCGGCG
GTGCCGGCGGACAAGGCCAGGTCCCCGGCGGTGCCGCTGCTGCTGCCGCCCGCGTTGGCG
GAGACTGTGGGCCCGGCGCCGCCTGGGGTCCTGCGCTGCGCCCTGGGGGACCGCGGCCGC
GTGCGGGGCCGCGCTGCGCCCTACTGCGTGGCCGAGCTCGCCACAGGCCCCAGCGCGCTG
TCCCCACTGCCCCCTCAGGCCGACCTTGATGGGCCTCCGGGAGCTGGCAAGCAGGGCATC
CCGCAGCCGCTGTCGGGTCCGTGCCGGCGAGGATGGCTCCGGGGCGCCGCCGCCCCCCGC
CGCCTGCAGCAGCGACGCGGGTCCCAACCAGAAACCCGCACAGGCGACGACGACCCGCAC
CGGCTTCTGCAGCAGCTAGTGCTCTCTGGAAACCTCATCAAGGAGGCCGTGCGAAGGCTT
CATTCGCGACGGCTGCAGTTACGTGCAAAGCTTCCCCAACGCCCGCTCCTGGGACCTCTG
TCGGCCCCGGTGCATGAACCCCCTTCGCCTCGCAGCCCTCGCGCGGCCTGCAGTGACCCT
GGCGCCTCCGGGAGGGCGCAGCTCAGAACTGGCGACGGCGTTCTTGTGCCTGGCAGCTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1008
Start on reference sequence 169
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 9 42
recombination site loxP site loxP recombination site 1015 1048
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : FRAT1
Symbol Nomenclature : FRAT1
Designation : FRAT-1|frequently rearranged in advanced T-cell lymphomas 1|proto-oncogene FRAT1
Full Nomenclature : frequently rearranged in advanced T-cell lymphomas
GENEID : 10023
Locus Tag : RP11-452K12.1
GI : 60654376
GenBank Accession : AY892962
HGNC : 3944
MIM : 602503
Vega : OTTHUMG00000018848
Target GenBank: BC034476

 

Reference Sequence Alignment

  

HsCD00005253      ATGCCGTGCCGGAGGGAGGAGGAAGAGGAAGCCGGCGAGGAGGCGGAGGGGGAGGAAGAG
NM_005479.3       ATGCCGTGCCGGAGGGAGGAGGAAGAGGAAGCCGGCGAGGAGGCGGAGGGGGAGGAAGAG
                  ************************************************************

HsCD00005253      GAGGAGGACAGCTTCCTCCTACTGCAGCAGTCAGTGGCGCTGGGCAGCTCGGGCGAGGTG
NM_005479.3       GAGGAGGACAGCTTCCTCCTACTGCAGCAGTCAGTGGCGCTGGGCAGCTCGGGCGAGGTG
                  ************************************************************

HsCD00005253      GACCGGCTGGTGGCCCAGATCGGCGAGACGCTGCAGCTGGACGCGGCGCAGCACAGCCCG
NM_005479.3       GACCGGCTGGTGGCCCAGATCGGCGAGACGCTGCAGCTGGACGCGGCGCAGCACAGCCCG
                  ************************************************************

HsCD00005253      GCCTCGCCGTGCGGGCCCCCGGGGGCGCCGCTGCGGGCCCCGGGGCCCCTGGCTGCGGCG
NM_005479.3       GCCTCGCCGTGCGGGCCCCCGGGGGCGCCGCTGCGGGCCCCGGGGCCCCTGGCTGCGGCG
                  ************************************************************

HsCD00005253      GTGCCGGCGGACAAGGCCAGGTCCCCGGCGGTGCCGCTGCTGCTGCCGCCCGCGTTGGCG
NM_005479.3       GTGCCGGCGGACAAGGCCAGGTCCCCGGCGGTGCCGCTGCTGCTGCCGCCCGCGTTGGCG
                  ************************************************************

HsCD00005253      GAGACTGTGGGCCCGGCGCCGCCTGGGGTCCTGCGCTGCGCCCTGGGGGACCGCGGCCGC
NM_005479.3       GAGACTGTGGGCCCGGCGCCCCCTGGGGTCCTGCGCTGCGCCCTGGGGGACCGCGGCCGC
                  ******************** ***************************************

HsCD00005253      GTGCGGGGCCGCGCTGCGCCCTACTGCGTGGCCGAGCTCGCCACAGGCCCCAGCGCGCTG
NM_005479.3       GTGCGGGGCCGCGCTGCGCCCTACTGCGTGGCCGAGCTCGCCACAGGCCCCAGCGCGCTG
                  ************************************************************

HsCD00005253      TCCCCACTGCCCCCTCAGGCCGACCTTGATGGGCCTCCGGGAGCTGGCAAGCAGGGCATC
NM_005479.3       TCCCCACTGCCCCCTCAGGCCGACCTTGATGGGCCTCCGGGAGCTGGCAAGCAGGGCATC
                  ************************************************************

HsCD00005253      CCGCAGCCGCTGTCGGGTCCGTGCCGGCGAGGATGGCTCCGGGGCGCCGCCGCCCCCCGC
NM_005479.3       CCGCAGCCGCTGTCGGGTCCGTGCCGGCGAGGATGGCTCCGGGGCGCCGCCGCCTCCCGC
                  ****************************************************** *****

HsCD00005253      CGCCTGCAGCAGCGACGCGGGTCCCAACCAGAAACCCGCACAGGCGACGACGACCCGCAC
NM_005479.3       CGCCTGCAGCAGCGACGCGGGTCCCAACCAGAAACCCGCACAGGCGACGACGACCCGCAC
                  ************************************************************

HsCD00005253      CGGCTTCTGCAGCAGCTAGTGCTCTCTGGAAACCTCATCAAGGAGGCCGTGCGAAGGCTT
NM_005479.3       CGGCTTCTGCAGCAGCTAGTGCTCTCTGGAAACCTCATCAAGGAGGCCGTGCGAAGGCTT
                  ************************************************************

HsCD00005253      CATTCGCGACGGCTGCAGTTACGTGCAAAGCTTCCCCAACGCCCGCTCCTGGGACCTCTG
NM_005479.3       CATTCGCGACGGCTGCAGTTACGTGCAAAGCTTCCCCAACGCCCGCTCCTGGGACCTCTG
                  ************************************************************

HsCD00005253      TCGGCCCCGGTGCATGAACCCCCTTCGCCTCGCAGCCCTCGCGCGGCCTGCAGTGACCCT
NM_005479.3       TCGGCCCCGGTGCATGAACCCCCTTCGCCTCGCAGCCCTCGCGCGGCCTGCAGTGACCCT
                  ************************************************************

HsCD00005253      GGCGCCTCCGGGAGGGCGCAGCTCAGAACTGGCGACGGCGTTCTTGTGCCTGGCAGCTTG
NM_005479.3       GGCGCCTCCGGGAGGGCGCAGCTCAGAACTGGCGACGGCGTTCTTGTGCCTGGCAGCTAA
                  **********************************************************:.

Pathways

NCI : Presenilin action in Notch and Wnt signaling
Reactome : Beta-catenin phosphorylation cascade
Reactome : Degradation of beta-catenin by the destruction complex
Reactome : disassembly of the destruction complex and recruitment of AXIN to the membrane
Reactome : Signal Transduction
Reactome : Signaling by Wnt
Reactome : TCF dependent signaling in response to WNT
WikiPathway : DNA damage response (only ATM dependent)
WikiPathway : Wnt Signaling Pathway
WikiPathway : Wnt Signaling Pathway and Pluripotency

Protein

UniProt : Q92837
HPRD : 03935

Clone

Cloning Information : 135178
HIP Master Clone ID : 111678
Original Clone ID : FLH135178.01L

Organism


TAX_ID : 9606
Species Specific ID: 10023

Genome

Chromosome : 10
Map Location : 10q24.1
Ensembl : ENSG00000165879

Reagents

Labome : FRAT1-antibody