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MGC33182 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CSNK1A1L
Gene Name:  casein kinase 1, alpha 1-like
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH159253.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 15928075
Title: Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Authors: HIP
HsCD00005282

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1014nts         Open reading frame : 1 to 1014
Reference Sequence Alignment

ATGACAAACAACAGCGGCTCCAAAGCCGAACTCGTTGTGGGAGGGAAATACAAACTGGTG
CGGAAGATCGGGTCTGGCTCCTTTGGAGACGTTTATCTGGGCATCACCACCACCAACGGC
GAGGAAGTAGCAGTGAAGCTGGAATCTCAGAAGGTCAAGCACCCCCAGTTGCTGTATGAG
AGCAAACTCTACACGATTCTTCAAGGTGGGGTTGGCATCCCCCACATGCACTGGTATGGT
CAGGAAAAAGACAACAATGTGCTAGTCATGGACCTTCTGGGACCCAGCCTCGAAGACCTC
TTTAATTTCTGTTCAAGAAGGTTCACCATGAAAACTGTACTTATGTTAGCCGACCAGATG
ATCAGCAGAATTGAATACGTGCATACAAAGAATTTTCTACACCGAGACATTAAACCAGAT
AACTTCCTGATGGGTACTGGGCGTCACTGTAATAAGTTGTTCCTTATTGATTTTGGTTTG
GCCAAAAAGTACAGAGACAACAGGACCAGGCAACACATACCGTACAGAGAAGATAAACAC
CTCATTGGCACTGTCCGATATGCCAGCATCAATGCACATCTTGGTATTGAGCAGAGCCGC
CGAGATGACATGGAATCCTTAGGCTACGTTTTCATGTATTTTAATAGAACCAGCCTGCCG
TGGCAAGGACTAAAGGCTATGACAAAAAAACAAAAATATGAAAAGATTAGTGAGAAGAAG
ATGTCCACCCCTGTTGAAGTTTTATGTAAGGGGTTTCCTGCAGAATTCGCCATGTACTTG
AACTACTGTCGTGGGCTGCGCTTTGAGGAAGTCCCAGATTACATGTATCTGAGGCAGCTA
TTCCGCATTCTTTTCAGGACCCTGAACCACCAATATGACTACACATTTGATTGGACGATG
TTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCAGGGTCAGCAGGCCCAA
ACCCAGACAGGCAAGCAAACTGAAAAAAACAAGAATAATGTGAAAGATAACTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1429
Start on reference sequence 416
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CSNK1A1L
Symbol Nomenclature : CSNK1A1L
SYNONYM : CK1
Designation : CKI-alpha-like|casein kinase I alpha S-like|casein kinase I isoform alpha-like
Full Nomenclature : casein kinase 1, alpha 1-like
GENEID : 122011
GI : 61354486
GenBank Accession : AY888060
HGNC : 20289
Vega : OTTHUMG00000016748
Target GenBank: BC028723

 

Reference Sequence Alignment

  

HsCD00005282      ATGACAAACAACAGCGGCTCCAAAGCCGAACTCGTTGTGGGAGGGAAATACAAACTGGTG
NM_145203.5       ATGACAAACAACAGCGGCTCCAAAGCCGAACTCGTTGTGGGAGGGAAATACAAACTGGTG
                  ************************************************************

HsCD00005282      CGGAAGATCGGGTCTGGCTCCTTTGGAGACGTTTATCTGGGCATCACCACCACCAACGGC
NM_145203.5       CGGAAGATCGGGTCTGGCTCCTTTGGAGACGTTTATCTGGGCATCACCACCACCAACGGC
                  ************************************************************

HsCD00005282      GAGGAAGTAGCAGTGAAGCTGGAATCTCAGAAGGTCAAGCACCCCCAGTTGCTGTATGAG
NM_145203.5       GAGGACGTAGCAGTGAAGCTGGAATCTCAGAAGGTCAAGCACCCCCAGTTGCTGTATGAG
                  *****.******************************************************

HsCD00005282      AGCAAACTCTACACGATTCTTCAAGGTGGGGTTGGCATCCCCCACATGCACTGGTATGGT
NM_145203.5       AGCAAACTCTACACGATTCTTCAAGGTGGGGTTGGCATCCCCCACATGCACTGGTATGGT
                  ************************************************************

HsCD00005282      CAGGAAAAAGACAACAATGTGCTAGTCATGGACCTTCTGGGACCCAGCCTCGAAGACCTC
NM_145203.5       CAGGAAAAAGACAACAATGTGCTAGTCATGGACCTTCTGGGACCCAGCCTCGAAGACCTC
                  ************************************************************

HsCD00005282      TTTAATTTCTGTTCAAGAAGGTTCACCATGAAAACTGTACTTATGTTAGCCGACCAGATG
NM_145203.5       TTTAATTTCTGTTCAAGAAGGTTCACCATGAAAACTGTACTTATGTTAGCCGACCAGATG
                  ************************************************************

HsCD00005282      ATCAGCAGAATTGAATACGTGCATACAAAGAATTTTCTACACCGAGACATTAAACCAGAT
NM_145203.5       ATCAGCAGAATTGAATACGTGCATACAAAGAATTTTCTACACCGAGACATTAAACCAGAT
                  ************************************************************

HsCD00005282      AACTTCCTGATGGGTACTGGGCGTCACTGTAATAAGTTGTTCCTTATTGATTTTGGTTTG
NM_145203.5       AACTTCCTGATGGGTACTGGGCGTCACTGTAATAAGTTGTTCCTTATTGATTTTGGTTTG
                  ************************************************************

HsCD00005282      GCCAAAAAGTACAGAGACAACAGGACCAGGCAACACATACCGTACAGAGAAGATAAACAC
NM_145203.5       GCCAAAAAGTACAGAGACAACAGGACCAGGCAACACATACCGTACAGAGAAGATAAACAC
                  ************************************************************

HsCD00005282      CTCATTGGCACTGTCCGATATGCCAGCATCAATGCACATCTTGGTATTGAGCAGAGCCGC
NM_145203.5       CTCATTGGCACTGTCCGATATGCCAGCATCAATGCACATCTTGGTATTGAGCAGAGCCGC
                  ************************************************************

HsCD00005282      CGAGATGACATGGAATCCTTAGGCTACGTTTTCATGTATTTTAATAGAACCAGCCTGCCG
NM_145203.5       CGAGATGACATGGAATCCTTAGGCTACGTTTTCATGTATTTTAATAGAACCAGCCTGCCG
                  ************************************************************

HsCD00005282      TGGCAAGGACTAAAGGCTATGACAAAAAAACAAAAATATGAAAAGATTAGTGAGAAGAAG
NM_145203.5       TGGCAAGGACTAAGGGCTATGACAAAAAAACAAAAATATGAAAAGATTAGTGAGAAGAAG
                  *************.**********************************************

HsCD00005282      ATGTCCACCCCTGTTGAAGTTTTATGTAAGGGGTTTCCTGCAGAATTCGCCATGTACTTG
NM_145203.5       ATGTCCACCCCTGTTGAAGTTTTATGTAAGGGGTTTCCTGCAGAATTCGCCATGTACTTG
                  ************************************************************

HsCD00005282      AACTACTGTCGTGGGCTGCGCTTTGAGGAAGTCCCAGATTACATGTATCTGAGGCAGCTA
NM_145203.5       AACTACTGTCGTGGGCTGCGCTTTGAGGAAGTCCCAGATTACATGTATCTGAGGCAGCTA
                  ************************************************************

HsCD00005282      TTCCGCATTCTTTTCAGGACCCTGAACCACCAATATGACTACACATTTGATTGGACGATG
NM_145203.5       TTCCGCATTCTTTTCAGGACCCTGAACCACCAATATGACTACACATTTGATTGGACGATG
                  ************************************************************

HsCD00005282      TTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCAGGGTCAGCAGGCCCAA
NM_145203.5       TTAAAGCAGAAAGCAGCACAGCAGGCAGCCTCTTCCAGTGGGCAGGGTCAGCAGGCCCAA
                  ************************************************************

HsCD00005282      ACCCAGACAGGCAAGCAAACTGAAAAAAACAAGAATAATGTGAAAGATAACTTG
NM_145203.5       ACCCAGACAGGCAAGCAAACTGAAAAAAACAAGAATAATGTGAAAGATAACTAA
                  ****************************************************:.

Pathways

Panther : Hedgehog signaling pathway
Panther : Parkinson disease
Panther : Wnt signaling pathway

Clone

Cloning Information : 159253
HIP Master Clone ID : 134788
Original Clone ID : FLH159253.01L

Organism


TAX_ID : 9606
Species Specific ID: 122011

Genome

Chromosome : 13
Map Location : 13q13.3
Ensembl : ENSG00000180138

Reagents

Labome : CSNK1A1L-antibody