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cdoA (Bacillus subtilis) in pET15_NESG (His-tagged bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  cdoA
Gene Name:  cysteine dioxygenase
Sequence             
Original Clone ID: SR112-14.1
Keyword: targeted domain
Species: Bacillus subtilis
Type: cDNA
Vector Name: pET15_NESG               Format:  CLOSED
Source: Northeast Structural Genomics Consortium
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Northeast Structural Genomics Consortium
BsCD00530862

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AVAILABLE
No restriction
Special MTA: PSI/NESG

 

Insert sequence: 486nts         Open reading frame : 1 to 486

ATGGAACTGTATGAGTGTATCCAAGACATTTTTGGCGGCTTGAAAAATCCATCGGTTAAA
GATTTAGCAACGTCTTTAAAACAAATTCCAAACGCAGCAAAATTGAGTCAACCGTATATT
AAGGAACCAGACCAGTACGCTTACGGCCGAAATGCCATCTATCGAAATAATGAATTGGAA
ATTATCGTGATTAACATTCCGCCAAACAAGGAGACAACAGTACACGATCATGGTCAATCC
ATTGGTTGTGCAATGGTGTTAGAAGGAAAGCTTCTTAATTCTATTTATCGTTCAACCGGC
GAACACGCAGAACTCTCCAATTCATACTTTGTCCACGAAGGAGAATGCCTTATTTCAACC
AAAGGTTTAATTCACAAAATGTCCAATCCAACATCTGAACGAATGGTGTCTCTTCATGTC
TACTCCCCTCCTTTGGAAGACATGACGGTCTTTGAGGAACAAAAGGAGGTATTGGAAAAT
TCATAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGCCATCACCATCACCATCACAGCCAT

3' Linker Sequence:
CTCGAGATCCGGCTGC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in SR112
Recommended expression in: E. coli BL21
       DyNA Vector Map

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Vector Name: pET15_NESG

pET15_NESG in advanced viewed

Synonyms: pET15, pET15-modified, pET15Cc, pET15_NESG
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.
Comments: Modified Novagen pET vector-common MCS
Size (bp): 5723
Parent Vector: None
Empty Vector: EvNO00336943
Properties: bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name: Northeast Structural Genomics Consortium
Gaetano T. Montleione
Jessica Bearden
Publications: PMID: 15808222
Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI) 327 452
bacterial origin ori ColE1 origin of replication 3881 4563
gene fragment ROP repressor of primer ROP sequence 3281 3457
primer T7 forward T7 forward sequencing primer 481 496
primer T7 reverse T7 reverse sequencing primer 257 275
promoter T7 T7 promoter 478 497
repressor binding site lacO lacO regulatory sequence 456 478
selectable marker AmpR ampicillin resistance gene 4661 5230
tag His N-terminal 6x His tag 387 404
trxn termination sequence T7 term T7 termination sequence 257 275

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : cdoA
GENEID : 938837
Locus Tag : BSU31140
GI : 16080166
Target GenBank: None

 

Protein

PDB - Structural Annotation : 3EQE
Structural Annotation Wiki : SR112
UniProt : O32085_BACSU
Protein Structure Viewer

FirstGlance in Jmol
PDB ID

Style



Color





Clone

Cloning Information : 539734
HIP Master Clone ID : 450054
Original Clone ID : SR112-14.1

Experimental

Structural Biology Knowledgebase : SBKB (3EQE)
TargetTrack - Experimental data : SR112

Organism


TAX_ID : 224308
Species Specific ID: 938837

Reagents

Labome : cdoA-cDNA