TM0010 (T. maritima )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  TM0010
Gene Name:  NADP-reducing hydrogenase, subunit C
Original Clone ID:
VR94-21.5
Keyword:
None
Species:
Thermotoga maritima
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
TmCD00531177
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1821nts
Open reading frame: 1 to 1821

ATGCCGCTCACAACGAACACGATCCTTATCTGTGCAGGTGGAGCGTGTATTTCCGCCGGC
GAAAAGAGTGTCAAAGATGCCTTCGAAGAAGAATTGAGAAAGTACGGCCTCGATGAAGTT
GTCAGGGTGATAGAGACCGGTTGTATGGGAGCGTGTACATTGGGACCGATTGCCGTGATC
TACCCGGAAAGTGTGTTTTACCAGAAACTCACACCAGACGCAGCAAGAGAGATCGTCGAA
GAACACATTTTGAAGGGAAGGATCGTGGAGAAGTACCTTTACAAGGCTTCCGAAGGAAAA
CCTGTTCCAAGAGTTCATGAGGAAGTGCCTTTCTTCAAGAAACAGGTTAGGATCGTCACA
AGGAATCTCGGTGTCATAGATCCCACGAAGATAGAGGAGTACATCGCAAGAGACGGATAT
TTTGCCCTTGCGAAGGCTCTTCAGATGAAACCGGAAGACGTGATAAAGGAGATAAAGGAC
AGCGGACTCAGAGGAAGAGGAGGAGCGGGTTTTCCAACAGGGCTCAAGTGGGAACTCGCG
GCAAGGCAAAAGGCCGAGAAGAAATTCGTTGTCTGTAACGCGGACGAAGGAGACCCGGGT
GCTTTCATGGACAGATCGGTTCTTGAAGGAGATCCACACGCCGTCATAGAGGGGATGGCA
ATAGCCGCCTACGCGATAGGTGCTCAGAAGGGGTTCGTTTATGTCAGGGCGGAGTATCCT
CTCGCCATTGAAAGGCTCAACATAGCGCTGAATCAGGCAAGAGAGTACGGTTTCCTTGGA
GGAAACATAATGGGAACGGATTTTTCCTTCGATATAGAGATCAGAATCGGTGCGGGTGCG
TTCGTTTGTGGTGAGGAAACCGCTTTGATGGCCTCCATAGAGGGTGAGAGAGGCCAACCG
AAGGTGAAGCCTCCCTATCCCGTTGAGAAGGGACTCTGGGGATACCCCACAGTGATCAAC
AACGTTGAAACACTTGCGAACGTACCCTGGATCATCAGAAACGGTGCGAAAGAGTTCAGA
AAATACGGGACGGAGAACTCTCCGGGAACGAAAGTATTCGCTCTCGCCGGTAAAGTGAAG
AACACAGGTCTTGTGGAAGTTCCCATGGGAATCACACTCAGAGAGCTCATATACGAGATT
GGAGGAGGAATAGCCGGAGACAAAAAGCTGAAAGCCATCCAGACTGGGGGACCGAGCGGA
GGGTGTATCCCCGCTGAGTACGTAGACACACCTGTTGATTTCGAATCCCTCCAGAAACTC
GGTGCAATCATGGGTTCTGGTGGAATGATAGTGATGGACGAAGACGACTGTATGGTTGAC
GTCGCAAGGTTCTTCCTCGAGTTCACCGTGGAAGAATCCTGTGGAAAATGTACGCCCTGT
AGAGAAGGAACGAAGAAGATGCTCGAAATCCTGGAGAAGATCACTTCCGGAGAGGGAACA
GAGGAGGACATAGAAGAGCTCGAAAAGCTGGCGCATGTGGTAAAGGACACTTCCCTCTGT
GGACTTGGTCAAACCGCACCGAATCCTGTTCTTTCCACACTCAGATACTTCAGAGACGAG
TATCTGGCGCATGTCAAAGAGAAGAGGTGTCCATCCAAGAAGTGTAAAGCGTTGATCAGC
TACGTGATTGATCCTGAAAAATGTGTTGGATGTACCGCGTGTGCGAGGGTCTGTCCGGTT
CAGTGTATAAGCGGTCAGGTGAGACAGCCTCACGTGATAGATCAGGCGGAGTGTGTCAGG
TGTGGTAGCTGTATCGAAGTCTGTAGATTTGGAGCTATAAGCAAGGTTACTCCAGCTCTG
CCGGTGGAGGAGGCGGTAGAA

Protein sequence predicted based on nucleotide sequence

MPLTTNTILICAGGACISAGEKSVKDAFEEELRKYGLDEVVRVIETGCMGACTLGPIAVI
YPESVFYQKLTPDAAREIVEEHILKGRIVEKYLYKASEGKPVPRVHEEVPFFKKQVRIVT
RNLGVIDPTKIEEYIARDGYFALAKALQMKPEDVIKEIKDSGLRGRGGAGFPTGLKWELA
ARQKAEKKFVVCNADEGDPGAFMDRSVLEGDPHAVIEGMAIAAYAIGAQKGFVYVRAEYP
LAIERLNIALNQAREYGFLGGNIMGTDFSFDIEIRIGAGAFVCGEETALMASIEGERGQP
KVKPPYPVEKGLWGYPTVINNVETLANVPWIIRNGAKEFRKYGTENSPGTKVFALAGKVK
NTGLVEVPMGITLRELIYEIGGGIAGDKKLKAIQTGGPSGGCIPAEYVDTPVDFESLQKL
GAIMGSGGMIVMDEDDCMVDVARFFLEFTVEESCGKCTPCREGTKKMLEILEKITSGEGT
EEDIEELEKLAHVVKDTSLCGLGQTAPNPVLSTLRYFRDEYLAHVKEKRCPSKKCKALIS
YVIDPEKCVGCTACARVCPVQCISGQVRQPHVIDQAECVRCGSCIEVCRFGAISKVTPAL
PVEEAVE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in VR94
Recommended expression in:
Not Applicable