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PSPTO_3170 (P. syringae pv. tomato str. DC3000 ) in pMCSG7

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Explanation of Terms


Gene: Gene Symbol:  PSPTO_3170
Gene Name:  glycosyl transferase family protein
Sequence             
Original Clone ID: APC63609.1_SG314_pMCSG7
Keyword: partial cds
Species: Pseudomonas syringae pv. tomato str. DC3000
Type: cDNA
Vector Name: pMCSG7               Format:  CLOSED
Source: Midwest Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Midwest Center for Structural Genomics
Shonda Clancy
PsCD00531255

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1275nts         Open reading frame : 38 to 1312

TCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCCCGAGTACGGCATGCGCGCAGC
GTTGAATACCGCGCGTGAGTCCATCGTTCTGCTGCGTAACGAAAACACTGCGGCGGGCAA
ACCGCTGCTGCCACTGGCGCGCTCGGCGAAGATCGCCGTGATCGGCAACTGGGCACATGA
AACACCTGCGTCACCATTCGGCACGGCCAACTCGCCGCCCAACAGCTACGTCACCGAACT
GAGCGGCCTGCAGCAACTGGCGTCCAACAGCACCGACGTCACCTACCTCCCGGAAATGAG
CCTGAACCCGGCGAATTCGACCTGGTATCAGCCAGCCACGGGTAACAACAGCATCAGCAA
TTCCGGGGTTAAAGCCGAGTATTTCTCCAACACCAGCTTTTCCGGCGATCCGGCACTGAC
GCGCGTCGAGCCAGGCGTAAACCTGAACTGGGCCACCGGCACCAACGTCACCAATGCGGG
CAGCACGGCCGTGTCGGGCTTTAGCCCTTCAGCCGGTGCCTTCTCGGCCCGCTTCACGGC
GACCATCAAGCCGACTGTCTCGGGCGCACAGGTGTTCAAGGTTCGCGCCGATGGCCCTTA
CAAGCTGTGGGTCAACGATGAGCTGGTACTGCAGAGCGATGGCGTGCCTTACTCAGGCGA
CGTCGTGAACGCGCTGACAACGTCGGGGAAAACTGCTGAATTGAGTGCTGGCAAGACCTA
CACCGTAAAACTTGAATACCAGCGCATTCAGGGCAATTTCATTCCTGTGCTGGGCGGCCT
GACCGGTGTGCAGATGAGCTGGGCATCGTTGCGTCCGCCGAAGGATCTGTCGAGCTACGA
TGCGGTCGTGGTTGCCACCGGCACTACGTCTGAAAACGAAGGCGAAGGTTCTGACCACGG
TTTCGATCTGCCTGACCAGCAAGCAGAGCTGATCAGCTACGTCACCAAGGCCAACCCCAA
TACCATCGTGGTCATGCATGGCGGTGGTGTGGCCAACATGCAACCGTGGGCGAACAAGGT
CGGCGCGACGCTGCAAGCCTGGTTCCCGGGTCAGCAAGGCGGGCAGGCTCTGGCCGAGAT
TCTGTATGGCAAGGTCAACCCGTCGGGCAAACTGCCGATCACCATCGACAAGAAAATCGA
GGATAACCCAAGCTACGCGTCCTACCCGGACCCGGCGGCCTATCGTGGCGACAATGCTCT
GACCGAAATGACCTACAGCGAAGGCCTGTACATGGGTTATCGCGGTTATGACAAGAAGCA
CGCCAAGCCGCTGTACCCGTTCGGTTACGGGCTGTCCTACACCACGTTCTAACATTGGAA
GTGGATAACGGATCCGAAT


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
c25t,L9L
                                

5' Linker Sequence:
GCC

3' Linker Sequence:
TAAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC63609.1
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pMCSG7

pMCSG7 in advanced viewed

Synonyms: pMCSG-7
Sequencing Primer: Forward:  T7short
Reverse:  T7 terminator
Description: Bacterial expresson vector with T7 promoter, adds N-terminal His tag and TEV protease site; amp resistance; ligation independent cloning (LIC).
Comments: pET21 derivative; for routine HTP purification
Size (bp): 5286
Parent Vector: None
Empty Vector: EvNO00084271
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: Midwest Center for Structural Genomics
Publications: PMID: 12071693
Title: A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site

PMID: 18988021
Title: A family of LIC vectors for high-throughput cloning and purification of proteins.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS LIC ligation-independent cloning (LIC) region 204 235
bacterial origin ori ColE1 pBR322-type bacterial origin of replication 3280 3280
promoter T7 T7 promoter 360 376
protease cleavage site TEV TEV cleavage site cds 223 243
repressor binding site lac operator LacO fragment 335 357
repressor protein gene LacI LacI coding region 890 1849
selectable marker AmpR ampicillin resistance gene 4038 4898
tag His 2 N-terminal 6xHis tag 268 285
tag His C-terminal 6xHis tag 140 157
trxn termination sequence T7 term T7 terminator sequence 26 72

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : PSPTO_3170
SYNONYM : PSPTO3170
GENEID : 1184827
Locus Tag : PSPTO_3170
GI : 28853588
Target GenBank: None

 

Protein

SMART domain : PA14 :
Structural Annotation Wiki : APC63609.1

Clone

Cloning Information : 576078
HIP Master Clone ID : 481677
Original Clone ID : APC63609.1_SG314_pMCSG7

Experimental

TargetTrack - Experimental data : APC63609.1

Organism


TAX_ID : 223283
Species Specific ID: 1184827

Reagents

Labome : PSPTO_3170-cDNA