Dde_2494 (D. alaskensis G20 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Dde_2494
Gene Name:  Fic family protein
Original Clone ID:
31252
Keyword:
full-length cds
Species:
Desulfovibrio alaskensis G20
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
DdCD00531343
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1029nts
Open reading frame: 51 to 1079

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGATCAAGC
CCTCCAACTACCAGCCACCCTACACCATTACCCCGGCGATAGTGAATCTGGTCGCCGAGA
TAGGCGAGATCATCGGCCGCTACACCGTGCTGGCCGAACAGAACCTGACGCCGCGCCTGC
GCCGGGAGAACCGCATCCGTACCATCCAGGCCTCGCTGGCCATTGAGAACAATACGCTCA
CCCTTGAACAGGTGACCGCCGTGATCGATGGCAAACGGGTGTTGGGTCACCCCCGCGAGA
TTCAGGAGGTGCGTAACGCCTTTGCGACCTACGAGGCCATGGAGGACTGGGACGCCAGTG
TCGAGGGGGATTTGCTGGCGGCACACGAGTTGTTGATGCGTGGGCTGGTGGATGAAACGG
GTCGATATCGATCCGGCGGGGTCGGCATCTTCCGGGGAGAGCAGTTAGTTCACATGGCTC
CTCCTGCTGATCGTGTGCCCAAGCTTATGGCGGATCTCCTGGATTGGCTGGAAAACACCA
ACGAGCATCCCTTGGTGGCCAGTTGCATTTTTCACTACGAGTTCGAATTCATCCACCCCT
TTGCCGACGGTAACGGCCGCATGGGTCGGTTGTGGCAAACCCTGATTCTACGAAACTGGA
AGCCGCTGCTGGCCTACCTGCCGGTGGAGACAGTGATCCGTGATCGCCAGGAAGATTATT
ACCGGGTACTCGCGGTCGCCGACAGCCAAGCGGATGCCACTCCTTTTGTCGAGTTCATGT
TGGGGGCGTTGCGCGATGCCGTGCGCGAGGCTGTGTCGACCGACCATGTAGGCGACCAAG
TAACCGACCAAGTAGCGGCGTTGATCCGTGCGATTGGGGGCGGTGAGCTGAGCAGCAACG
ATTTGATGCAGGCTTTAGGTTTGTCACACCGGCCCACGTTCCGTAACAACTACCTCAACC
CGGCCATGGAAGACGAATGGATCGAACGTACCCAACCCGATTCCCCGCGCAGCCCGACTC
AGCGCTATCGGTTGACCGGTAAAGGCCAGCGTTGGCTGCAACCCCATGCCGACGAGTAA

Protein sequence predicted based on nucleotide sequence

MIKPSNYQPPYTITPAIVNLVAEIGEIIGRYTVLAEQNLTPRLRRENRIRTIQASLAIEN
NTLTLEQVTAVIDGKRVLGHPREIQEVRNAFATYEAMEDWDASVEGDLLAAHELLMRGLV
DETGRYRSGGVGIFRGEQLVHMAPPADRVPKLMADLLDWLENTNEHPLVASCIFHYEFEF
IHPFADGNGRMGRLWQTLILRNWKPLLAYLPVETVIRDRQEDYYRVLAVADSQADATPFV
EFMLGALRDAVREAVSTDHVGDQVTDQVAALIRAIGGGELSSNDLMQALGLSHRPTFRNN
YLNPAMEDEWIERTQPDSPRSPTQRYRLTGKGQRWLQPHADE*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a1013c,H338P
                                                    

5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGC
3' Linker Sequence:
CGCGACTTAATTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAG

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 374857
Recommended expression in:
Not Applicable