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Mfla_0188 (Methylobacillus flagellatus KT) in pSpeedET (bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  Mfla_0188
Gene Name:  peptidoglycan-binding LysM
Sequence             
Original Clone ID: 26139
Keyword: full-length cds
Species: Methylobacillus flagellatus KT
Type: cDNA
Vector Name: pSpeedET               Format:  CLOSED
Source: Joint Center for Structural Genomics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: PSI
Joint Center for Structural Genomics
MfCD00531414

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AVAILABLE
No restriction
Special MTA: PSI

 

Insert sequence: 1053nts        
Open reading frame : 1 to 1053

ATGTATAAATACATTATAACGCTGCTCATGCTTTGTTGCCCGATTTTGCCTGTCCTGGCG
GATGAGGTAAAGCTGCAACCTCATCATCCTGATCGGTATGTCGTGGTAAAAGGGGATACG
TTATGGGGAATCGCTGGGCGCTTTCTCAAAGATCCCTGGCAGTGGCCGCAGGTATGGAAA
ATGAACCGCGAGCAGATCAAGAACCCTCATTTGATCTATCCTGGCGATGTCGTGATGCTC
GATATGAGTAGCGGTTCTCCGCAATTGCGCCTGCTGCGCGAAACGGTGGCCAATCCCGAG
GTAACCTTAACTCCTGGCATTCGTACTGAACAGCTGGATGTCGAAGCAATCCCGACGATT
TCTCCCCGTATCATCGCTCCTTTCCTGAGCCAGCCGCTGATCATCGAAAATGACGGCCTC
AAAGACGCGCCAAGGATTATCGCAGGACCGGAAAACCGCGTGGTACTGAGTCCGGGCTCC
CGCATCTATGTCGACAAGATTGACGAAGGGGAGGGAACCTTGTGGAATATCTACCGTACG
GGCAAGACGTATATCGACCCCGAGACCAAGGCAGTACTCGGTACGGAGGCGATTTACCTG
GGAGATGCAAGGATTAGCAAGTATGGTGAGCCTGCCAGTGGCGATATCGTCAGCGCCAAG
GAAGAGATATTCGCCAACGATAAACTGGTGGCAGCGCCCGAGAGCCTGCAAACCAATTTT
GTGCCGCAGGCGCCGGAGGACGATGTCCAGGGGCGCATCCTCTCCATCTATGGCGGGGTG
GCTGAAGCCGGGCGGAATACCATCATTGCCATCAGCAAGGGAAGTGACGACGGGCTGGAG
CAAGGACATGTGCTTGCTATCAACCGTAATGGCCGGGTCATTAAGAACCCTGGTTACGAC
AAGAAGAACAAGAAAGGCTCAGAGAACATCAAGCTCCCCAACGAGCGTATCGGGCTCGCC
ATGGTGTTCCGCACTTTCGACAAGATATCCTACGCGCTGATCATGCAGGCCACCGAACCA
GTCAATGTGCTGGATGTGGTGGAGAAGCCATAA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
ATGGGATCTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGC

3' Linker Sequence:
CGCGACTTAATTAAACGGTCTCCAGCTTGGCTGTTTTGGCGGATGAG

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 368635
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pSpeedET

pSpeedET in advanced viewed

Synonyms: pSpeed-ET
Sequencing Primer: Forward:  pBAD forward
Reverse:  alt_bad_rev
Description: Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.
Comments: If this vector contains an insert, the ccdB death cassette will not be present.
Size (bp): 5464
Parent Vector: None
Empty Vector: EvNO00285791
Properties: PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name: Joint Center for Structural Genomics
Publications: PMID: 18004753
Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ori ColE1-type origin of replication 3306 3988
inducible trxn activator protein gene araC araC coding sequence 4563 5438
negative selection marker ccdB ccdB death cassette gene (lost in with-insert form) 1596 1805
promoter T7 T7 promoter 290 310
promoter araC araC promoter 256 285
protease cleavage site TEV TEV protease cleavage site 382 402
selectable marker CmR chloramphenicol resistance gene (CmR) (lost in with-insert form) 502 1158
selectable marker KanR kanamycin resistance gene 2396 3208
tag His N-terminal 6xHis tag 364 381
trxn regulatory element arabinose O1 operator arabinose O1 operator 161 182
trxn regulatory element arabinose O2 operator arabinose O2 operator 5 23
trxn termination sequence rrnB rrnB T2 transcription terminator 2258 2284

 

  • Gene
  • Protein
  • Clone
  • Experimental
  • Organism
  • Reagents

Gene

 

Gene Symbol : Mfla_0188
GENEID : 3999488
Locus Tag : Mfla_0188
GI : 91774544
GenBank Accession : YP_544300
Target GenBank: None

 

Protein

SMART domain : LysM : Lysin motif
Structural Annotation Wiki : 368635

Clone

Cloning Information : 290299
HIP Master Clone ID : 219785
Original Clone ID : 26139

Experimental

TargetTrack - Experimental data : 368635

Organism


TAX_ID : 265072
Species Specific ID: 3999488

Reagents

Labome : Mfla_0188-cDNA