BVU_0362 (P. vulgatus ATCC 8482 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BVU_0362
Gene Name:  hypothetical protein
Original Clone ID:
72357
Keyword:
SP or TM removed
Species:
Phocaeicola vulgatus ATCC 8482
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
BvCD00531859
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1539nts
Open reading frame: 51 to 1589

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCCAAGACAAAG
TGGAATGTTGGGACCGGTTTGAGTTGAGTTTTAAACAGGTGACCAAAGGGAATCCTTTTG
ATATACGTCTTTCCGCTACTTTCGTTTGTGGAAAGGAAAAGAAAACGGTGGAAGGATTTT
ATGATGGTGAGAATACTTATAGGATACGGTTTATGCCTGCTGTCGCCGGGGAATGGAGGT
ATGTTACATCTTCCAGTATAGGGGCTATGAATGGTCGGAAAGGTACATTCACAGTGATAC
CTGCCGGAAAAGATAATCATGGCATGGTATTGGTGGACGGAGAACATAATTTTAAATATG
CGGACGGTACCCGTTATTATCCGATGGGTACGACGGCCTATGCATGGACACATATGAAGG
AAACGACTCAGGAGGCTACTTTGAAATCGTTTGGTGAAGCCGGTTTTAATAAGGTAAGGA
TGTGTGTGTTTCCTAAAAACTATTCTTTAGTGAAAGACGAGCCTGCGCTTTATCCTTTTG
AAATAGAGAAGACGATAAAAGATAAGGAAGGAAATGAAAGGAAAGAATGGGACTTTGACA
GATTTGACCCGGCTTTTTTTCAGCATTTGGAGAAACGGATAGACCAACTGAACCGTTTGG
GGATTGAGGCGGATTTGATCCTTTTCCATCCTTATGATAAGGGAAGATGGGGCTTTGATG
CTATGTCGAATGAGGTGAATGTACGTTATATTAAATATATTACTGCTCGTTTGGCTTCTT
TTAGAAATGTATGGTGGTCTATGGCAAACGAGTGGGATTATGTAAAAGCGAAAACTGTGG
ACGATTGGAAGCTGTTGACGAAAACTGTGGTTGAGAATGATCCTTATCGCCATTTATGTT
CTATTCATGGAGCGACGGCTACTTATTTTGATTATTGGATGCCTGAATTTACCCATGTCA
GCATACAGGATGAGGCACCGGTACTGAGTTCTACCGCTTCTGCTACTTTAAGAAAAATTT
ATCGCAAACCGGTTATATGTGATGAGGTTGGGTATGAAGGGAATCTACCTTATCGTTGGG
GAAGGTTAAGTCCGCAGCAGATGACTTGCTTTATTTTGAATGGTTTGTTGGGTGGAATTT
ATGTCACTCACGGTGAATGTTATCAGCAGGGGAACGAACCTATCTTTTGGGCGCAAGGGG
GATCTTTAAAAGGTGAAAGCTGGAAACGTGTGAAATTTTTAAGAACGATTATTGAAGCTG
CTCCCCATCCGTTGGAGATGGCAGATATCAGTCGTGACTTGGTTACTTCTACTGCTGGAC
CAGATTATTATCTGGTCAATATGGGAAAAGATGTGAAAGGTTTCTGGACTTTTAATCTGC
CGGTAAAAAATGCGGATTATAACAAATTGCAAAAGAATAAACGGTTTAAAGTGGAGATTA
TTGATGTGTGGGCCATGACTGTCACGGAATATCCTGTGATATTTGAAACAACGGAGGAAT
TGGATTATCGTGTTTTTGATATACATCATCGGGGAGTAAGAATTCCGGATGCTCCTTATA
TTGTTCTTAGAATAACAGAAGTGAAATAACGCGACTTAATTAATTTGGCGGATGAGAGAA
GATTTTC

Protein sequence predicted based on nucleotide sequence

QDKVECWDRFELSFKQVTKGNPFDIRLSATFVCGKEKKTVEGFYDGENTYRIRFMPAVAG
EWRYVTSSSIGAMNGRKGTFTVIPAGKDNHGMVLVDGEHNFKYADGTRYYPMGTTAYAWT
HMKETTQEATLKSFGEAGFNKVRMCVFPKNYSLVKDEPALYPFEIEKTIKDKEGNERKEW
DFDRFDPAFFQHLEKRIDQLNRLGIEADLILFHPYDKGRWGFDAMSNEVNVRYIKYITAR
LASFRNVWWSMANEWDYVKAKTVDDWKLLTKTVVENDPYRHLCSIHGATATYFDYWMPEF
THVSIQDEAPVLSSTASATLRKIYRKPVICDEVGYEGNLPYRWGRLSPQQMTCFILNGLL
GGIYVTHGECYQQGNEPIFWAQGGSLKGESWKRVKFLRTIIEAAPHPLEMADISRDLVTS
TAGPDYYLVNMGKDVKGFWTFNLPVKNADYNKLQKNKRFKVEIIDVWAMTVTEYPVIFET
TEELDYRVFDIHHRGVRIPDAPYIVLRITEVK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 418078
Recommended expression in:
Not Applicable